• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.4 no.8
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• pp.5-15
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• 1983

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.570-577
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• 2011

We developed insect-resistant GM rice(Bt transgenic rice) by inserting the mCry1Ac1 a modified gene from the soil bacterium, Bacillus thuringiensis. The Bt transgenic rice expressing the Bttoxin mCry1Ac1 was tested for the effects on survival of Misgurnus anguillicaudatus and Cyprinus carpio, commonly used as a model organism in ecotoxicological studies. M. anguillicaudatus and C. carpio fed 100% ground rice in suspension, using either Bt rice or non-GM counterpart rice(Nakdong). The Bt rice used for the test were confirmed to have the mCry1Ac1 gene expression by the immuno-strip and ELISA analysis. Feeding test showed that no significant differences in cumulative immobility and abnormal response of M. anguillicaudatus and C. carpio fed on between Bt rice and non-GM counterpart rice. The 96hr-LC₅₀ values showed no difference between Bt rice(>1,000mg/L) and non-GM rice(>1,000mg/L). We concluded that there was no significant difference in toxicity for non-target organisms(M. anguillicaudatus and C. carpio) between Bt rice and non-GM counterparts.

• 농업기술회보
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• v.51 no.2
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• pp.28-29
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• 2014

친환경 안전 농산물에 대한 관심이 높아지면서 환경 친화적인 병해충 방제법에 대한 관심이 커지고 있다. 해충의 친환경 방제 수단인 미생물 살충제의 이용에 대한 관심이 높아지면서 국내에서도 이에 대한 연구가 활발히 진행되고 있다. 그 중에서도 화학농약에 대한 약제 저항성 발현으로 방제가 어려운 가루이류의 살충성 곰팡이를 이용한 방제법 개발은 친환경 농산물 생산에 크게 기여할 것으로 기대된다.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.10 no.2 s.89
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• pp.65-70
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• 1989

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.365-373
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• 2005

This study was carried out for looking into the status of susceptibility of pest insects to insecticides. Each insect Brown planthopper(BPH), Green leaf hopper(GLH), Smaller brown plant hopper(SBPH), Rice water weevil(RWW), were captured at various areas where the host crops were being cultivated and the susceptibility level of each pest insect was investigated. The susceptibility of each pest insect varied by insect species and areas where they were caught. BPHs kept higher level of susceptibility comparing to susceptible reference strain except to most of tested insecticides except imidacloprid (Resistance ratio was 68). The susceptibilities of GLH and SBPH to most of insecticides for their control did not developed markedly since 1976 except fipronil and imidacloprid which is widely used for WRR control. The insecticides used for control of WRR were very effective even at the concentration of one fourth of recommending concentration, but in 2000 suwon strain of WRR showed markedly reduction of susceptibility to carbofuran.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.10a
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• pp.267-267
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• 2017

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.10 no.4 s.91
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• pp.68-73
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• 1989

살충제는 그 종류가 많고 또 그 종류에 따라 작용기구도 다르기 때문에 화학적 계열이 서로 다른 각 살충제의 작용기구를 이해한다는 것은 그 약제를 보다 유효하고 합리적으로 사용하는데 있어 큰 지침이 된다. 또한 방제 효과를 저하시키는 원인이 되고있는 해충의 살충제에 대한 저항성 발달은 살충제의 작용 기구와는 어떤 상관이 있으며 그 방제대책은 무엇인지 이번호부터 2회에 걸쳐 소개한다.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.18-26
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• 2013

Genetically modified (GM) rice Agb0101, which expresses the insecticidal toxin modified cry1Ac (mcry1Ac1) gene, was developed by the Rural Development Administration in Korea. To monitor the probable release of Agb0101 in the future, it is necessary to develop a reliable detection method. Here, we developed the PCR detection method for monitoring and tracing of GM rice. The primer pair (RBEgh-1/-2) from a starch branching enzyme (RBE4) gene was designed as an endogenous reference, giving rise to an expected PCR amplicon of 101 bp. For the qualitative PCR detection, construct- and event-specific primers were designed on the basis of integration sequence of T-DNA. Event-specific PCRs amplified specifically 5'- or 3'-junction region spanning the native genome DNA and the integrated gene construct, while none of amplified product was shown on crops, rice varieties, and other insect-resistant transgenic rice lines. The event-specific real-time PCR method was performed using TaqMan probe and plasmid pRBECrR containing both rice endogenous gene RBE4 sequence and 5'-junction sequence as the reference molecule. The absolute limit of quantification (LOQ) of real-time PCR was established with around 10 copies for one plasmid molecule pRBECrR. Thereafter, the different amounts of transgenic rice (1, 3, 5, and 10%, respectively) were quantified by using the established real-time PCR method, with a range below 19.55% of the accuracy expressed as bias, 0.06-0.40 of standard deviation (SD) and 3.80-7.01% of relative standard deviations (RSD), respectively. These results indicate that the qualitative and quantitative PCR methods could be used effectively to detect the event Agb0101 in monitoring and traceability.

• Journal of Life Science
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• v.22 no.12
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• pp.1614-1620
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• 2012

Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens St${\aa}$l) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated. The integration, expression, and inheritance of this gene in the $R_1$ and $R_2$ generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the $R_1$ and $R_2$ transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the $R_2$ transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA into rice plants therefore can help to control insect pests.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.5 no.9
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• pp.14-25
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• 1984