• Journal of Navigation and Port Research
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• v.37 no.2
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• pp.165-171
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• 2013

YoungDo bridge is the first suspension bridge in Busan which experienced several times repair and reinforcement after completion in 1934. The bridge is under demolition for extension and restoration work since 2011. The purpose of this research is to establish data base about modern concrete. The mix design of the concrete is like that cement : fine aggregate : coarse aggregate = 1 : 2 : 4 or 1 : 3 : 6 by the parts. The compressive strength is in the ranges from 50 to 55 MPa and the elastic modulus is in the ranges from 25 to 35 GPa. From the fact that the deviation is less than 10 %, considerably high quality control was conducted in those days. The carbonation depth is 5.92 cm at the bridge post and 14.3 cm at the machine room. That is why ocean environment keeps the high humidity. The diffusion coefficient of chloride ion through the pores in concrete is 1.052e-12 $m^2/s$ from the regression analysis using the experiment data. The water cement ration is estimated at approximately 35 % in case if the concrete using ordinary Portland cement.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.2
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• pp.1-10
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• 2019

Based on the land system of Joseon Dynasty and contemporary perception of land, this study analyzed the reason Yun seon-do entered Bogildo Island and a characteristic of Buyong-dong garden. According to this analysis, this study interpreted the purpose that Yun seon-do had built Buyong-dong garden at a socio-economic perspective. The land such as Sarlimchontack(山林川澤) is recognized as a commonland in the Joseon Dynasty. Except the reserved area(禁山), however, actions to make commonland private through Ib-an(立案) and protecting graves was frequent. Bogildo Island was a place difficult to build a private garden because the island had been appointed a reserved forest to protect pine trees. Right after Byungjahoran, Yun seon-do entered the island on the pretext of protecting forests, and built Buyong-dong garden. The island was easy to move to manage private farms as well as possessed abundant forest resources and resources that have economic value such as fish and salt. It indicates that, unlike his pretext, economic benefits have also been considered.

• Journal of Life Science
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• v.33 no.10
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• pp.808-819
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• 2023

This study was conducted to develop a selection marker for the identification of the Bacillus licheniformis K12 strain in microbial communities. The strain not only demonstrates good growth at moderate temperatures but also contains enzymes that catalyze the decomposition of various polymer materials, such as proteases, amylases, cellulases, lipases, and xylanases. To identify molecular markers appropriate for use in a microbial community, a search was conducted to identify variable gene regions that show considerable genetic mutations, such as recombinase, integration, and transposase sites, as well as phase-related genes. As a result, five areas were identified that have potential as selection markers. The candidate markers were two recombinase sites (BLK1 and BLK2), two integration sites (BLK3 and BLK4), and one phase-related site (BLK5). A PCR analysis performed with different Bacillus species (e.g., B. licheniformis, Bacillus velezensis, Bacillus subtilis, and Bacillus cereus) confirmed that PCR products appeared at specific locations in B. licheniformis: BLK1 in recombinase, BLK2 in recombinase family protein, and BLK3 and BLK4 as site-specific integrations. In addition, BLK1 and BLK3 were identified as good candidate markers via a PCR analysis performed on subspecies of standard B. licheniformis strains. Therefore, the findings suggest that BLK1 can be used as a selection marker for B. licheniformis species and subspecies in the microbiome.