• 농업생명과학연구
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• 제43권3호
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• pp.35-44
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• 2009

알로에 껍질과 육질 추출물이 Collectrichum gloeosporioides, Fusarium oxysporum, and F. solani의 세 가지 식물병원균에 대한 알로에 추출물의 용매 및 농도별 항균활성을 분석하였다. 용매의 종류에 따른 알로에 추출물의 항진균 활성은 C. gloeosporioides, F. solani에 대하여 물>메탄올>에탄올의 순서로 항균활성이 높았다. 알로에 껍질과 육질추출물의 항균활성에 있어서 F. oxysporum은 껍질 추출물에서, C. gloeosporioides와 F.sloani는 육질 추출물이 활성이 높아 껍질 보다는 육질에서 우수한 항균활성을 보였다. 알로에 육질의 물 추출물 농도에 따른 항균활성은 F. oxysporum는 $100{\mu}l/L$의 농도에서는 항균활성이 나타나지 않았으나 $100,000{\mu}l/L$의 농도에서 9.5%의 항균율을 보인 반면, C. gloeosporioides $100{\mu}l/L$의 농도에서 활성반응을 시작하여 $100,00{\mu}l/L$의 농도에서 최고 29.3%의 항균율을 나타내었다. F. oxysporum에 대한 알로에 육질 추출물 엽면살포 처리의 방제효과는 육질 추출물 농도가 증가할수록 방제효과가 증가하는 경향이었으며, 10g/L 농도 엽면살포 처리는 대조구에 비하여 62%의 방제효과를 나타내어 추출물의 농도가 증가함에 따라 In vivo에서도 F. oxysporum에 대한 항균활성이 강하게 나타내어 예방차원에서의 알로에 육질 추출물 엽면살포 처리는 F. oxysporum에 의한 시들음병 방제에 효과적인 수단이 될 수 있다고 사료된다.

• Environmental Analysis Health and Toxicology
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• 제18권2호
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• pp.131-136
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• 2003

Anti-fungal materials producing bacteria were isolated from soil by bennett's agar and actinomycete isola-tion agar medium. The bacterla were identified as synonym of Actinomycetes. Based on the data obtained from its morphological and colony characteristics. The medium for production of anti-fungal materials was YEME (yeast extract 4 g, malt extract l0g, glucose 4 g, D.W 1ι, pH 7.0${\pm}$0.2). The culture conditions were 30$^{\circ}C$, 7 days and 200 rpm in shaking incubator. No. 13, No. 15 and No.28 strains were produced anti-fungal materials against fungal plant pathogens. Specially, The No. 28 strain showed a powerful biopesticide activity and broad spectrum effects of anti -fungal materials on Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

• 한국미생물·생명공학회지
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• 제28권6호
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• pp.334-340
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• 2000

In order to select multifunctional powerful antagonistic biocontrol agent against red-pepper rotting fungi Phytophthora capsici, we isolated an indigenous antagonistic bacterium which produces antifungal substances and siderophores from a local soil of Kyongju, Korea. The isolated strain was identified as Pseudomonas fluorescens biotype F. The antibiotic produced from P. fluorescens 2112 inhibited hyphae growth and the zoospore germination of Phytophthora capsici. The favorable carbon, nitrogen source and salts for the production of antibiotic from P. fluorescens 2112 were glycerol, beef extract and LiCi at 1.0%, 0.5% and 5 mM, respectively. And antagonistic activity of P. fluorescens 2112 was confirmed against P. capsici in vivo.

• 한국미생물·생명공학회지
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• 제27권3호
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• pp.191-196
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• 1999

A multifunctional antagonistic bacterium, producing both antifungal antibiotic and chitinase that could be used as biocontrol agents against fungal plant pathogens was isolated from the plant-disease suppressive soil. The isolate was identified as Promicromonospora sp. KH-28 from various morphological, biochemical and physiological tests. The antifungal antibiotic produced by Promicromonospora sp. KH-28 was soluble in n-butanol, methanol, toluene, n-hexane, ethanol but insoluble in H2O, acetone, chloroform, ethylacetate and ethylether. It inhibited the growth of fungal plant pathogens of Fusarium solani, F. oxysporum, Alternaria mali and Phytophthora capsici. The antagonistic Promicromonospora sp. KH-28 produced optimally the antifungal antibiotic when it was cultivated at pH 7, 3$0^{\circ}C$ for 5 days.

• 한국미생물·생명공학회지
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• 제25권3호
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• pp.335-337
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• 1997

In this study, we show a convenient MTT assay for detect the susceptibility of yeast-like form of Trichosporon beigelii against antifungal agents. This assay was developed based on mitocondrial respiration by determining reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to formazan. Cells of T beigelii are seeded into 96-well microtiter plates, and antifungal agents, amphotericin B, magainin and CA-ME hybrid peptide were added with various concentrations. After 24 hr incubation, MTT was added, then incubations were continued for 4 hr. Formazan formation was quantified photometrically after extraction of the formazan with acid sodium dodesyl sulfate (SDS). From this assay, we could obtained MICs of antifungal agents against T. beigelii. The presented method can easily be used as an effective methods to assess the antiftingal action of various agents on yeasts with minimal amounts of antifungal agents.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.664-670
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• 1996

The aim of the present research program was to develop a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among over 440 isolates, a strain (NA-4803) producing antifungal substance against Trichophyton spp. Nannizzia otae and Pyricularia oryzae was selected. The strain NA-4803 was identified as strain similar to Streptoverticillium blastmyceticum with respect to morphological and physiological characteristics, lecithinase and lipolytic activity, degradation of organic compounds, resistance to antibiotics and utilization of carbon and nitrogen sources. But it showed some differences such as positive reaction of nitrate reduction, negative reaction of L-tyrosine degradation, resistance to cephaloridine, and utilization of I -rhamnose and inulin. The strain NA-4803 was named as Streptoverticillium sp. NA-4803.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.349-353
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• 1999

Antagonistic Promicromonospora sp. KH-28 isolated from a suppressive soil could produced a chitinase and a antifungal antibiotic for the biocontrol ability. The chitinase and the antibiotic appeared to inhibit plant pathogens of Fusarium oxysporum. Phytophthora capsici, Alternaria kiki, fusarium solani, Stemphylium sp., and Psudomonas fluorescens. the antibiotic produced from the strain was identified as a antifungal substance of 503 dalton having a pyrimidine skeleton with an aliphatic side chain. The Promicromonospora sp. KH-28 was able to suppress effectively F. oxysporum derived-fusarium wilt of red-pepper plant in the pot in vivo test.

• 환경위생공학
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• 제13권2호
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• pp.40-46
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• 1998

For a biological control on a plant pathogen, Pryicularia Oryzae, and a mosquito, Aedes aegypti, Bacillus thuringiensis strain AF6 which produces parasporal inclusion, delta-endotoxin, was isolated. The B. thuringiensis strain AF6 was produced not only an antifungal substance(AFS) against P. oryzae, but also a mosquitocidal delta-endotoxin. The AFS of the strain AF6 in more stable at pH 4.0 than pH 10.0. At the mode of action, the AFS of the strain AF6 was inhibited hypha growth on potato agar plate(pH 5.0), and degraded cell walls of P. oryzae.

• Biomolecules & Therapeutics
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• 제3권3호
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• pp.182-187
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• 1995

The clastogenecity and mutagenicity of antifungal 6-[(N-halophenyl)amino]-7-chloro-5, 8-quinolinedione (RCK 3, 7, 13, 14, and 15) had been evaluated. Salmonella typhimurium reversion assay (Ames test) was used to test the mutagenicity of RCKs. RCK14 was mutagenic in S. typhimurium(TA98 and TA100) with and without rat liver microsomal activation. Whereas RCK3, 7, 13 and 15 were negative in Ames test with Salmonella typhimurium(TA98 and TA100), The clastogenecity was tested on the RCKs with in vivo mouse micronucleus assay. All of RCKs tested did not show any clastogenic effect in mouse peripheral blood. Thus RCKs were not supposed to cause any chromosomal damage termed micronuclei. These results indicate that RCK 3, 7, 13 and 15 have no genotoxic potential under these experimental condition.

• 한국미생물·생명공학회지
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• 제30권1호
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• pp.21-25
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• 2002

토양으로부터 분리한 Bacillus sp. YJ-63으로부터 항진균성 항생물질을 분리하여 그 구조를 분석한 결과는 다음과 같다. 항생물질은 7개의 $\alpha$-amino acid와 1개의 $\beta$-amino acid가 결합한 환상구조를 가지는 iturin E 계에 속하며 특히. $\beta$-amino acid의 methylene 수가 기존의 iturin E 보다 더 많은 것으로 나타났다.