• Title/Summary/Keyword: 항산화효소

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Preparation and Characteristics of Agarooligosaccharide (한천 올리고당의 제조 및 특성)

  • Cho, Suk-Hyung;Cho, Jea-Chul;Kim, Young-Jun
    • Proceedings of the KAIS Fall Conference
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    • 2010.11b
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    • pp.1010-1013
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    • 2010
  • 한천 올리고당은 효소인 아가라제에 의하여 제조하였으며 제조에 있어서 효소의 농도, 반응 온도 등에 따른 최적 제조조건을 규명하였으며 한천 올리고당을 GPC로 분자량을 측정하였을 때 분자량이 약 4,200 Da이었다. 또한 한천올리고당의 항산화 작용 및 아질산 소거능을 측정한 결과 항산화 효과가 비타민 C보다는 약간 낮지만 68%정도의 비교적 높은 항산화 활성을 나타내었으며 한천 올리고당이 이산화질소의 과잉생성을 억제하여 정상레벨로 유지하는 것을 알 수 있었다.

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이달의 과학자-강원대 축산가공학과 이성기 교수

  • Korean Federation of Science and Technology Societies
    • The Science & Technology
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    • v.31 no.7 s.350
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    • pp.80-81
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    • 1998
  • 축산물의 가공과 이용에 관한 연구를 진행 하고 있는 강원대 축산가공학과 이성기 교수는 '냉동 세절돈육에서 소금이 지방산화 촉진과 항산화효소 역가에 미치는 영향'이라는 논문을 발표해 세계적인 관심을 모으고 있다. 이교수는 이러한 연구를 통해 돈육에 첨가한 소금이 냉동저장중에 지방산화를 급증시키는 반변 돈육 속에 함유된 항산화 효소들의 역가는 감소시켰다고 밝히고 있다.

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Antioxidant Potential of Enzymatic Extracts from Blueberry (Vaccinium corymbosum L.) (블루베리 (Vaccinium corymbosum L.) 유래 효소 추출물의 항산화성)

  • Senevirathne Mahinda;Jeon You-Jin;Ha Jin-Hwan;Cho Somi K.;Kim Soo-Hyun
    • Journal of Life Science
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    • v.16 no.1
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    • pp.49-57
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    • 2006
  • Enzymatic extracts were prepared from the blueberry (Vaccinium corymbosum L.) collected in Jeju, Korea. Five carbohydrases namely AMG, Celluclast, Termamyl, Ultraflo and Viscozyme, and five proteases namely Alcalase, Flavourzyme, Kojizyme, Neutrase and Protamex were used to prepare the enzymatic extracts. Antioxidant properties of each extracts were studied using stable 1,1-diphenyl 2-picrylhydrazyl (DPPH), reactive oxygen species (ROS), nitric oxide (NO) scavenging, metal chelating assays and lipid peroxidation inhibitory activity in hemoglobin-induced linoleic acid system. The phenolic content of all enzymatic extracts was in the range of 517.85-597.96 mg/100 g dried sample. DPPH and NO${\cdot}$scavenging, and metal chelating assays exhibited prominent activities. Viscozyme showed the highest DPPH activity $(0.046{\pm}0.002\;mg/mL)$ while AMG Showed the highest activity in NO${\cdot}$scavenging $(0.339{\pm}0.011\;mg/mL)$. All the extracts exhibited strong metal chelating activities. Blueberry enzymatic extracts also showed relatively good activity in hydrogen peroxide scavenging. AMG showed the highest lipid peroxidation inhibitory activity $(0.28{\pm}0.01\;mg/mL)$ in hemoglobin-induced linoleic acid system. In this results, the blueberry, which has potential antioxidant components, may be a good candidate as a natural antioxidant source.

Activities of Antioxidant Enzymes in Serum of Rats Feeding Soybean Extract (콩 추출물 투여가 흰쥐 혈청의 항산화효소 활성에 미치는 영향)

  • Yun Hong-Tae;Moon Jung-Kyung;Park Keum-Yong;Kim Yang-Ho;Shin Mi-Kyung;Kim Yeung-Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.spc1
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    • pp.136-140
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    • 2005
  • This study was conducted to determine antioxidative enzyme activity in serum of rats as affected by soybean cultivar, extract feeding concentration and fat dietary. In all cultivars, activities of antioxidant enzymes in treatment of various fat compositions and cholesterol followed by feeding soy-extracts were higher than in nonfeeding control, however, no significant differences existed in statistically. The activities of SOD and GSHpx in serum of rats when fed with various fat dietary were higher in Geomjeongkong 1 than in Tawonkong and Hwangkeumkong. Enzyme activities of SOD and GSHpx with each fat compositions in treatment soy-extracts was no significant differences statistically. There was significant differences among cultivars and concentrations of soy-extracts in interaction effect. Also, the activities of GSHpx was significant differences among cultivars and concentrations of soy-extracts and fat composition in interaction effect.

Antioxidant Enzyme Activities and Soil Properties of Healthy and Declining Abies koreana (Wils.) in Mt. Halla (한라산 구상나무 건전개체와 쇠약개체의 항산화효소활성 및 토양특성)

  • Lim, Jong-Hwan;Woo, Su-Young;Kwon, Mi Jeong;Kim, Young Kul
    • Journal of Korean Society of Forest Science
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    • v.96 no.1
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    • pp.14-20
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    • 2007
  • In order to examine the differences in antioxidant enzyme activities which represent defence mechanism to stressful environments, and soil properties between healthy and declining (or unhealthy) trees, we selected three sites, Witseorum, Youngsil and Sungpanak (Jindallebat). Antioxidant enzymes including Ascorbate peroxidase (APX) and Glutathione Reductase (GR), forest soil properties including soil texture, soil pH, organic matter, total nitrogen, available phosphate, cation exchange capacity, exchangeable cation content and nutrient contents in leaves of Abies koreana (Korean fir) trees were analyzed. There were no significant differences between healthy and declining trees in GR activity. However, seasonal difference in antioxidant enzyme activity was observed. GR activity was lower in June and August than that of September. Soil chemical and physical properties of each site showed a tendency that organic content, total nitrogen content, available phosphorus, cation exchange capacity and cation content were lower at the site of declining trees than the site of healthy trees.

Air Pollution Effects on the Photosynthesis and Chlorophyll Contents of Street Trees in Seoul (대기오염 피해를 받은 서울시내 가로수의 엽록소함량과 광합성 특성)

  • 우수영;이성한;이동섭
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.6 no.1
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    • pp.24-29
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    • 2004
  • The purpose of this study was to identify the effects of air pollution on the street trees in Seoul. Ten Gingko biloba street trees were selected in both Jongno(polluted area as a control) and Yangjae(non-polluted area) to investigate chlorophyll contents, photosynthesis and antioxidant enzymes such as APX(Ascorbate peroxidase) and GR(Glutathione reductase). The sampling, measurements and analysis were conducted in August. Chlorophyll contents and photosynthesis of Gingko biloba grown in Yangjae were higher than those of Jongno in the August measurement. In contrast, APX and GR activities of Gingko biloba grown in Yangjae were lower than those of Jongno. The observed tree responses are considered to be atypical compensatory response to polluted locations.

The Effects of Pulsed Electromagnetic Fields on Blood components, Antioxidant enzymes and Reactive Oxygen in Hyperlipidemic Rats (맥동전자장이 고지혈증 흰쥐의 혈액 성분, 항산화 효소 및 활성 산소에 미치는 영향)

  • Bang, Hyun-Soo;Jeong, In-Ho;Lee, Sang-Deok
    • Journal of Digital Convergence
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    • v.12 no.7
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    • pp.349-356
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    • 2014
  • The present study was aimed to investigate the effects of the application of pulsed electromagnetic fields on the blood components associated hyperlipidemia, antioxidant enzymes and reactive oxygen. The subjects were divided into three groups: General Diet, High fat Diet, High fat diet and pulsed electromagnetic fields. Pulsed electromagnetic field was applied with pulsed electromagnetic energy therapy equipment. The Glucose, free fatty acids, triglycerides, cholesterol and insulin was used to measure hyperlipidemia-related blood components, and the GSH, GRD, XO and MDA was used to measure antioxidant enzymes and reactive oxygen. The applied pulsed electromagnetic field has improved the concentrations of blood components and increasing GSH and GRD, that were decreased due to a high-fat diet and by reducing the increased MDA and XO to a level of general diet group. Therefore, the applied pulsed electromagnetic field can be the effective treatment for changes in the blood composition of hyperlipidemic rats, enhancement of antioxidative activity and the inhibition of reactive oxygen.