• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.67-67
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• 2002

아열대성 식물 4종 (문주란, Crinum asiaticum var. japonicum; 박달목서, Osmanthus insularis; 죽절초, Chloranthus glaber; 파초일엽, Asplenium antiquum)을 대상으로 자연 환경요인의 변화에 의한 항산화 효소 (superoxide dismutase, peroxidase, catalase, ascorbate peroxidase)의 활성과 isoenzyme 패턴의 변화를 전기영동으로 조사하였다. 그 결과, peroxidase의 활성과 isoenzyme 패턴이 식물종이나 환경조건에 따라 가장 다양하게 나타났다. Peroxidase는 4종 모두에서 여름철보다 겨울철에 활성이 높았고 문주란, 박달목서, 파초일엽에서는 겨울철에 특이적으로 발현되는 isoenzyme들도 관찰할 수 있었다. Catalase는 문주란, 박달목서, 파초일엽에서 검출되었다. 문주란 잎에서는 겨울철에 비해 여름철에 다소 높은 활성을 보였으며, 박달목서와 파초일엽에서는 겨울철에 높은 활성을 나타내었다. 그리고 문주란과 박달목서에서는 겨울철에 새벽이나 밤보다 낮시간에 높은 활성을 보였는데 파초일엽에서는 낮시간의 catalase 활성이 낮았다. Superoxide dismutase는 문주란, 박달목서, 파초일엽에서 검출되었으며, 특히 박달목서에서는 겨울철에 높은 활성을 보였다. Ascorbate peroxidase는 문주란과 파초일엽에서 관찰되었으나 계절적으로 큰 차이가 없었으며, 겨울철에는 isoenzyme 패턴의 일주기적 변화가 관찰되었다. 이상의 결과, 종별로는 문주란, 파초일엽에서 4종의 항산화효소가 모두 검출되었고, 박달목서에서는 ascorbate peroxidase가, 죽절초에서는 peroxidase를 제외한 모든 항산화 효소가 검출되지 않았다. 식물종에 따라 또는 환경요인의 변화에 따라 항산화효소의 활성 또는 isoenzyme 패턴의 차이를 보이고 있지만 항산화효소의 계절적 그리고 일주기적 변화가 관찰되어, 본 연구에서 조사된 4종의 아열대성 식물이 자연환경 조건 하에서도 산화적 스트레스에 처하고 있는 것으로 보인다.

• Journal of Life Science
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• v.12 no.3
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• pp.349-356
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• 2002

In order to observe the bioactivity of ovariectomized rats, nonovariertoized (sham) group, ovariectomized (Ovx) group, ovariectomized ginseng total saponin (GTS)-treated (Ovx+ GTS) group and ovariectomized ginseng water extract (GW)-treated (Ovx+CW) group were made. We measured AST (L-aspartate aminotransferase) and ALT (L-alanin aminotransferase) in sera, and MDA (malondialdehyde:lipid peroxidation), SOD (superoxide dismutase), catalase, total-glutathione (GSH + GSSG) and GPx (glutathione peroxidase) in liver tissue total homogenates of rat. AST activity of serum in Ovx group was 2.11 times increased, but ALT activity was not changed compared to Sham group. In AST activity, they tend to decrease significantly in each substance such as GTS and GW administered group. Lipidperoxides of each fraction in Ovx group were highly increased compared to Sham group. Extracts of ginseng-treated group markedly inhibited lipid peroxidation by 62% ∼72%. And as the result of the measurements of SOD, catalase, total-glutathione and GPx which are antioxidant enzyme, antioxidant enzymes in Ovx group much lower than in Sham group. But they were significantly increased in each substance such as GTS and GW, administered group. Based on the results, it is supposed that more produced free radicals decreased antioxidant enzyme. And it is also thought that extracts of ginseng can inhibit aging by reducing antioxidant enzyme.

• Korean Journal of Environment and Ecology
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• v.16 no.1
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• pp.46-54
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• 2002

This study was conducted to diagnose the health of street trees with physiological characters, and to figure out the relation of physiological characters and cambial electrical resistances. Ginkgo biloba, Prunes serrulata and Salix koreensis were chosen in the Cheonan City. Soils under trees were collected to analyze dehydrogenase activities, and chlorophyll content, nitrate reductase and superoxide dismutase activities were analyzed from leaves sampled at the edge of crown in July. Cambial electrical resistances were measured in May, July and September, Soils with low dehydrogenase activity reflected the level of pollution. Chlorophyll content was the lowest in the leaves of P. serrulata at the Dongseo-street. Nitrate reductase activity of Ginkgo biloba was higher than P. serruluta and Satix koreensis. Nitrate reductase activity showed higher activity in the city than control(Independence Hall and Yonam College), but superoxide dismutase activity in the city lower than control. P. serruzatu in the Dongseo-street that cambial electrical resistance increase continuously during the growing season, showed the loss of vitality Cambial electrical resistance was negatively or positively correlated with nitrate reductase($r^2$=-0.566) and superoxide dismutase activity($r^2$=0.579). It was concluded that cambial electrical resistance might be suitable for diagnosing the tree health.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.75-81
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• 2015

Purpose: The present study was conducted to investigate whether the changes in structure and activity of antioxidative enzymes, superoxide dismutase(SOD) and catalase(CAT) present in the eyes appeared when they were repeatedly exposed to UV-A, and reveal the correlation of these changes. Methods: Each enzyme solution was prepared from the standardized SOD and CAT, and repeatedly exposed to UV-A of 365 min under the condition of 30 minutes, 1 hour and 2 hours a day over 1, 2, 3, 4 and 5 days. Structural denaturation of SOD and CAT induced by repeat UV-A irradiation was confirmed by the electrophoretic analysis, and their enzyme activity was determined by the colorimetric assay using the proper assay kit. Results: SOD exposed repeatedly to UV-A showed the polymerization pattern through the electrophoretic analysis when it was repeatedly exposed under the condition of at least 1 hour a day however, the change of its activity was found to be less than 12%. On the other hand, CAT repeatedly exposed to UV-A showed reduced size of the electrophoretic band which indicated a structure denaturation and its activity was significantly decreased. In the case of that the repeat exposure time was longer, CAT activity was completely lost even though some enzyme band was shown in the electrphoretic analysis. Conclusions: From these results, it was revealed that the degree and pattern in structural denaturation of antioxidative enzymes differently appeared according to the type of enzyme, and the degree of structural denaturation was not always consistent with the reduction in enzyme activity.

• Proceedings of the Korean Nutrition Society Conference
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• 1995.11b
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• pp.55-55
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• 1995

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2003.05a
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• pp.105-105
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• 2003

본 연구는 쇠고기 분쇄육에 천연항산화제인 pycnogenol, catechin 및 $\alpha$-tocopherol과 합성항산화제인 BHT를 처리한 후 3, 5, 7, 10일 동안 냉장저장하면서 색도 및 pH, TBA 값 및 지방산 조성의 변화와 항산화효소인 SOD 및 catalase 활성의 변화를 검토하였다. 명도는 catechin 처리군이 저장에 따른 색의 변화가 가장 작았으며, pH는 처리 당일 5.83~5.87의 비슷한 수준이었으나 저장함에 따라 저하되어 5일째에는 대조군이 약 0.45 수준으로 크게 감소한 것에 비하여 항산화제처리군은 약 0.2 수준의 감소로 대조군과 큰 차이를 나타냈다. (중략)

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.71-76
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• 1995

The effects of light on the activities of several antioxidative enzymes, catalase (CAT), superoxide dismutase(SOD), ascorbate oxidase(AO), and peroxidase(POD) were examined in the hairy root cultures of Phytolacca esculenta van Houtte induced by Agrobacterium tumefaciens $A_4$T. Activities of CAT, SOD, and AO were significantly decreased with incresing light intensity (500-2,000 lx). The activity of AO under high light condition (2,000 lx)was decreased by 92% compared to the dark condition. The activities of glutathoine peroxidase (GPO), ascorbate peroxidase (APO) and general POD were increased under lower light intensify below 500 lx. The activity of GPO under 2,000 lx was decreased by 85% compared to the dark condition. The activities of antioxidative enzymes were more decreased in blue light (400-500nm). The activities of antioxidative enzymes in blue light intensity were increased in lower light intensity below 30 lx, but decreased 21-70% under 200 lx. The activity of AO was decreased by 70% under 200 lx with increasing blue light intensity. Our results suggest that the activities of antioxidative enzymes in hairy roots might be inhibited by endogenous oxidants generated under the high blue light conditions.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.431-438
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• 1997

The influence of deficient(7${\mu}M$) and suffcient(1000${\mu}M$) magnesium(Mg) supply on the content of mg, chlorophyll, protein and the activity of superoxide dismutase(SOD), scavengers of superoxide radical($O_2$), and ascorbate peroxidase(AP), $H_2O_2$scavenging enzyme, and glutathione redutase(GR) were studied in pumpkin(Cudurbita moscata $D_{UCHESNE}$) plants over a 11-day period. Over the 11 days Period of growth in nutrient solution with sufficient and deficient Mg supply, the contents of Mg, chlorophyll protein and the activities of the antioxidative enzymes remained more or less constant in Mg-sufficient leaves. In Mg-deficient leaves, the contents of Mg, chlorophyll and protein was seriously decreased with time, however the activities of SOD, AP and GR highly enhanced compared to those of Mg-sufficient. The results indicated the stimulative effect of Mg deficiency on toxic oxygen species and scavenging enzymes in plants.

• Korean Journal of Environmental Biology
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• v.24 no.2 s.62
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• pp.119-125
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• 2006

Present study aims to study antioxidant enzyme activity due to turbidity change in various tissues of Carassius auratus. As for the changes of antioxidant enzyme activity in tissues of C. auratus pursuant to the raising period under 50, 100, and 150 NTU with turbid water levels, there was no great difference between 50 NTU and 100 NTU compared to a control (0 NTU), however, it demonstrated a relatively noticeable difference at 150 NTU high turbid water level. When considering the antioxidant capacity in tissues of C. auratus in terms of DPPH free radical scavenging activity, there was shown a high activity in gill and liver tissues, therefore, it is thought that there appears a non-enzymatic antioxidant reaction when C. auratus is reared under the condition of highly turbid water. As for the enzymatic antioxidant reaction of antioxidant enzyme activity got increased as turbid water level went higher in order of 50, 100, 150 NTU, and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transperase (GST), increased in all tissues except for an integument, up to 20th day when it was started to be reared, and they showed a gradual decrease as time passed by. However, since the activity of glutathione reductase (GR) and glutathione peroxidase (GPX) is very low in almost all tissues, it is thought that the role of those enzymes would be quite ignorable in the course of antioxidant process.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.331-339
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• 2001

목적 : 본 연구는 식풍해경(熄風解痙), 소산풍열(疏散風熱) 효능이 있는 백강잠이 치매에 미치는 영향을 알아보기 위하여 실험을 행하였다. 방법 : 치매 유발물질인 $amyloid{\beta}(A{\beta})$ 25-35를 흰쥐의 뇌 astrocyte에 처리한 후 대표적인 항산화 효소인 catalase, superoxide dismutase,glutathione peroxidase 및 glutathione-S-transferase의 활성 변화와 NO 생성 변화를 관찰하였다. 결과 $A{\beta}$ 25-35 처리로 catalase와 superoxide dismutase 활성이 현저히 감소하였으나 백강잠을 처리한 경우는 이들 효소 활성이 크게 증가하였다. 그리고, $A{\beta}$ 25-35의 농도에 의존적으로 증가된 NO 생성은 백강잠의 농도에 의존적으로 유의성 있게 억제되는 것으로 나타났다. 결론 : 백강잠은 항산화계 효소의 활성화 및 $A{\beta}$처리와 같은 치매유발 물질의 독성에 대한 적응능력 향상을 통하여 astrocyte를 보호하는 효능을 가지는 것으로 사료되며, 아울러 노인성 치매 등 임상적 응용에 그 효과가 기대된다.