• Journal of Life Science
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• v.33 no.10
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• pp.835-841
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• 2023

To determine the characteristics of propolis fermented with microorganisms, the optimal conditions for propolis extraction with ethanol were first reviewed, and the physicochemical properties of the extracted propolis were then investigated. The shaking and stationary extraction methods were tested. The flavonoid content was 6.40 mg/g when the stationary extraction method was used and 6.95 mg/g when the shaking extraction method was used. The ethanol concentration was then varied, and the use of 50, 60, 70, and 80% ethanol resulted in the total flavonoid content in the propolis samples reaching 6.75, 8.45, 7.45, and 7.75 mg/g, respectively. This indicated that a 60% (v/v) concentration was optimal. As the extraction time was increased from 1 to 3 hr, the flavonoid content slightly increased; however, extending the extraction step to more than 3 hr did not significantly affect the flavonoid content. In terms of the extraction temperature, the highest concentration of flavonoids (8.63 mg/g) was detected at 50℃. In the propolis samples, 5.80, 2.61, and 1.32 mg/g of fructose, sucrose, and rhamnose were detected, respectively. As for the amino acid content, 17 amino acids, including glutamic acid, were detected, and the total amino acid content was 2.49 mg/g.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.525-528
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• 2001

This study was conducted to estimate the contents of heavy metals in some health foods available on Korean markets. The samples were digested with microwave system, then analyzed using GF-AAS for the contents of lead (Pb), cadmium (Cd) and arsenic (As). The contents of mercury (Hg) were determined using a mercury analyzer. The average values of Hg, Pb, Cd and As in calcium (Ca) health foods were 0.007, 1.08, 0.02 and 0.48 mg/kg respectively. Those values in chitosan health foods were 0.001, 0.36, 0.01 and 0.03 mg/kg respectively. Those values in propolis health foods were 0.013, 4.96, 0.01 and 0.13 mg/kg, respectively. The health foods that contained cow bone powders had the highest lead contents. Based on the variation in lead contents of those products, it could be possible that they might be contaminated through raw materials and/or manufacuring process. Some propolis products were also very high in lead contents. There could be risks for some population, especially the aged who overtake those health foods, to have heavy intake of lead. Therefore, we established the lead standards of 3.0, 2.0 and 5.0mg/kg less than for Ca, chitosan and propolis health foods respectively, based on the Codex method.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.2
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• pp.185-198
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• 2019

Human tissue undergoes aging by the oxidant damage via structural change and its physical properties. The skin aging process is well known and many evaluations have been conducted. However, studies on hair aging were relatively few and thus care for aging hair is difficult. This study aims to fabricate an aging hair and identify anti-aging effect with known ingredient in anti-aging. First of all, physical properties of aging hair of age 60s by physiologically intrinsic factors were compared to those of the hair made by various extrinsic factors such as several chemical reactions and iteration numbers of the treatments. The extrinsic aging hair of this study relates to the less amount of lipid and to the hair of perm treated once accordingly, wherein several physical properties, preferably comprise roughness and tensile strength, present a novel concept of the intrinsic aging hair. The penetration of peptide into the aging hair was leading the extrinsic hair towards more structurally directed a younger hair. In addition to the structural change, the penetration of the peptide enhanced texture and tensile strength of the aging hair. These patterns have been also found in addition of propolis. For the first time, these qualitative studies exhibit that indeed our extrinsic aging hair well describes the anti-aging efficacy as a receptor for a cross-linker and the ingredients of human hair.