• Korean Journal of Food Science and Technology
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• v.45 no.3
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• pp.370-375
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• 2013

Propolis is a resinous mixture found in the tree buds, sap flows, and other botanical sources, which is used by honey bees in the construction of their hives. Antimicrobial effects of propolis were evaluated against Streptococcus mutans KCTC 3065, S. sobrinus KCTC 3308, S. sobrinus KCTC 5134, and Porphyromonas gingivalis KCTC 5352 by an agar diffusion assay. Sensitivity of these microorganisms to propolis was evaluated in broth containing different concentrations of propolis at $37^{\circ}C$, followed by observation using transmission electron microscopy (TEM). Propolis inhibited all oral microorganisms tested at the minimum inhibitory concentration (MIC) of $0.14mg/{\mu}L$ in the agar diffusion assay. Treatment with 0.06 and $0.22mg/{\mu}L$ of propolis had a bactericidal effect in a concentration- and treatment time-dependent manner against the tested microorganisms. TEM of propolis-treated S. mutans KCTC 3065 and P. gingivalis KCTC 5352 revealed structural damage of the cell membrane. The activity of propolis was affected by heat and pH treatment. The results indicate that propolis shows antibacterial activity against oral microorganisms and that it has potential for future applications in the food industry.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.251-260
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• 2023

This study was conducted to investigate the effect of propolis extract on chicken patty. the meat quality characteristics and storage properties of chicken patties without propolis extract were compared to those with 0.1%, 0.2%, and 0.4% propolis ethanol extract. The addition of propolis extract resulted in increased fat and ash content in the chicken patties. There were no differences in pH, water holding capacity, cooking loss, and texture profile analysis, indicating that the propolis extract did not negatively affect emulsification stability. However, sensory evaluation showed that the higher the concentration of propolis extract added, the lower the total preference of the chicken patties. Over a storage period, patties treated with propolis extract exhibited a lower total microbial count, and volatile basic nitrogen (VBN) content compared to those without propolis extract. Therefore, the addition of propolis to chicken patties does not reduce emulsion stability but improves storage properties. However, the unique flavor of propolis decreases the preference for chicken patties, so the amount must be considered when using it.

• Journal of Apiculture
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• v.34 no.3
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• pp.245-254
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• 2019

We investigated the anti-tumor effects and molecular mechanism of Brazil, China and Korean regional propolis on HeLa ovarian cancer cell line. Each propolis extracts was prepared by ethanol extraction method. Cytotoxicity of propolis extracts was determinated by EZ-cytox cell viability assay. To necessity of anti-tumor effect and molecular mechanism of propolis, we must be adjusting propolis concentration. Due to 100 ㎍/mL of propolis extract were reduced cell viability to less than 50%, we adjusted all of propolis concentration to 100 ㎍/mL. By Western blotting analysis, we confirmed that anti-tumor mechanism of Brazil, China and Korea regional propolis has significantly difference. All of propolis was activated apoptosis related molecules such as PARP, caspase-3. However, cell proliferation signaling molecules including Akt1, ERK and Bcl-2 were reduced the protein expression level. Especially, the expression of tumor suppressor protein p53 was significantly increased in propolis-treated group such as Gyeonggi, Chungbuk, Chungnam, Jeonbuk, Gyeongnam and China. The phosphorylation of Bax which as apoptosis indicator was appeared in propolis-treated group such as Gyeonggi, Gangwon, Chungnam, Gyeongbuk, China. In this results showed that the regional propolis has completely different mechanism in anti-tumor. Thus, propolis extracts may be useful source of functional materials on anti-cancer and it will be able to choose the suitable propolis for cancer therapy by analyzing individual characteristics.

• Food Science of Animal Resources
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• v.22 no.1
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• pp.77-80
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• 2002

The studies was conducted to investigate the antioxidation effect of Korean propolis. Oxidation period of soy oil was 17.5 hr, soy oil with propolis(1,000 ppm) was extended the length of oxidation period. The length of oxidation periods of soy oil with Yecheon, Youngwol, Brazilian, Chinese, and Australian propolis were 33.5, 35.4, 32.0, 33.1, and 27.1hours respectively. The length of oxidation period of lard with propolis 1,000ppm of Yecheon, Youngwol, Brazilian, Chinese and Australian were 191, 167, 296, 230, and 207hours, respectively compared to control(22.5hours). When compared to tocopherol as natural antioxidant and BHT as synthetic antioxidant were revealed 16.7 and 20.5hours, respectively. The length of oxidation period was not different when compared to tocopherol and Youngwol propolis with soy oil. Ascorbic acid and citric acid had synergistic effect to propolis with soy oil when 200ppm of propolis was added.

• Journal of Life Science
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• v.31 no.7
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• pp.686-697
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• 2021

Propolis is a resinous substance produced by honeybees, which they use to protect their hives. Honeybees produce propolis by mixing exudates from the various trees and plants with saliva and beeswax. It has been used since around 300 B.C. as a folk medicine to cure wounds. Propolis contains many physiologically active components, such as flavonoids, phenolic compounds, and beeswax. Because of its functional components, propolis has a wide spectrum of biological applications. The compounds in propolis and its biological activity can vary according to the location of nectar source and extraction method. Propolis is most commonly known for its anti-microorganism activity against bacteria, viruses, and fungi. Artepillin C and caffeic acid phenethyl ester (CAPE) have been identified as regulatory compounds that reduce inflammation and exert immunosuppressive reactions on T lymphocytes. Through its anti-inflammatory activity, propolis exhibits anti-tumor activity, including the inhibition of cancer cell proliferation, the blocking of tumor signaling cascades, and antiangiogenesis. However, for the more apply of propolis its analysis of nectar source, identifying of propolis compound, the molecular mechanism of propolis and the investigation of compounds synergistic effects are essential. In this study, we described the physiological activity of propolis isolated from honeybees.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.574-579
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• 2008

Propolis is the generic term for the resinous substance collected by honey bees from a variety of plant sources. In this study, we have assessed the immunomodulatory properties of propolis (P) and fermented-propolis (FP) in BALB/c mice. Mice were subjected to gavage once a day (for 14 days) with 50, 100, 200 mg/kg body weight P, FP, or vehicle. Lymphocytes were isolated from the spleen and mesenteric lymph nodes (MLN) and the immune cell proportions, proliferative activities, and cytokine production were evaluated. The P- and FP-administration induced similar, but differential, alterations in the percentage of immune cell populations and their biological functions, including cytokine production and NK cell cytotoxicity. The proportion of$ CD4^+$ and $CD8^+$ T cells in the spleen was increased slightly in the P- and FP-administered mice as compared to the vehicle-treated mice. In MLN, the percentage of $CD4^+$ T cells was increased significantly in the 200 mg/kg P-treated mice. The mice which were treated with P and FP evidenced significantly increased interferon-$\gamma$ and interleukin-4 production in concanavalin A-stimulated splenocytes, whereas the production of theses cytokines was not shown to be induced by P-treatment. In addition, NK cell activity was also increased dramatically by the administration of P and FP. Collectively, these findings showed that P and FP are wide-spectrum immunomodulators, which may modulate both innate and adaptive immune responses.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.71-71
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• 2003

프로폴리스는 꿀벌들이 수목류의 생장점 보호 물질이나 진액을 수집, 타액과 혼합하여 만드는 암갈색 등 여러 가지 색을 띄는 끈적끈적한 교질성 물질이며, 벌통에 침입한 것들의 잔해를 부패하지 않게 하기 위하여 사용되어지고, 벌통 내의 미생물들의 성장도 억제시키는 것으로 알려지고 있다. 프로폴리스는 동구권에서 민간의약품으로 널리 이용되고 있는 점 등으로 미루어 볼 때, 금후 국내산 프로폴리스에 대하여 충분한 연구 가치가 있을 것으로 기대되어지며, 아울러 국내에서도 점차 활발한 연구 분위기 확산뿐만 아니라 다양한 소재 개발에 관한 관심이 고조되고 있다. (중략)

• Journal of Apiculture
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• v.34 no.1
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• pp.1-6
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• 2019

Honey bees use propolis to defend against invaders and pathogens. Propolis, a resinous mixture, has been widely used for its antibacterial, antifungal and anti-inflammatory properties. This research was carried out to evaluate the propolis productivity in Apis mellifera lines to select superior honeybee lines. For the study, which were collected from three regions in Korea were evaluated for the productivity of propolis. The results showed that, the average propolis per colony was highest in the line P2 (10.1 g/hive), which was inbed line derived from isolated region, compared to other lines. We also measured brood development one the basis of population size of eggs, brood, and adult bees to further probe for relation between population size and propolis yield, in that larger population generally have more foragers. Also, adult population expended more greatly in the line P2 compared to that in the control.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.664-670
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• 2007

The propolis is natural product produced by honeybees and is known to have many biologically useful properties such as anti-microbial, anti-oxidative and anti-tumorigenic activity. However, its radio-protective property has not been well studied. To investigate radio-protective effect of propolis on mouse testis, mice were supplemented with propolis after 5 Gy irradiation. The histological changes of testis were detected by TEM. The results indicate that propolis may protect tissue deformation which is induced by 5 Gy of ionizing radiation. Furthermore, to elucidate the potential molecular mechanisms involved in radio-protective property of propolis, we performed microarray experiments using oligo DNA microarray. We found 65 up-regulated genes and 224 down-regulated genes, whose expression levels were affected more than 2-fold by propolis treatment in mice irradiated at 5 Gy. We confirmed microarray data with reverse transcription-PCR using gene specific primers. The results of RT-PCR are highly correlated with those of microarray. These results may help understanding molecular mechanisms of radioprotective effects by propolis in mouse model.

• Food Science of Animal Resources
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• v.23 no.2
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• pp.168-171
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• 2003

The study was conducted to investigate the antioxidative effect of various extracted propolis. After addition of those extracts to soybean oil at the same level, their antioxidative effects were compared by Rancimat test. The control without added antioxidant showed the shortest Antioxidative Index(AI). The AI of the general Water Extracted Propolis(GWEP) and boiling WEP(BWEP) added to soybean oil were 4.51 and 5.02, respectively. The oxidation period in the BWEP was longer than the ascorbic acid and the GWEP. This result indicated that BWEP had more antioxidative effect than GWEP.