• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.74-81
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• 2022

Brassica napus, an oil crop that produces rapeseed oil, is an allotetraploid (AACC, 2n = 38) produced by natural hybridization between B. rapa and B. oleracea. In this study, microspore was cultured using the F₁ developed from a cross between 'EMS26' line with high oleic acid content and 'J8634-B-30' lines. The flower bud size showing the nuclear development at the late uninucleate and binucleate stage with high embryogenesis rate was 2.6 ~ 3.5 mm. Microspores were cultured using only this size and after then most microspore embryo developed into secondary embryos and then regeneration plants obtained from the developed multilobe. The analysis of the ploidy of the plants revealed that 66.7% and 27.8% of the total lines were tetraploids and octoploids, respectively. The sizes of stomatal cells in tetraploids, octoploids, and diploids were 25.5, 35.6, and 19.9 ㎛, respectively, indicating that ploidy level was positively correlated with cell size. Furthermore, 62 tetraploid doubled haploid (DH) lines were selected. The average oleic acid (C18:1) and linolenic acid (C18:3) concentrations of DH were 72.3% and 6.2%, respectively. Oleic acid and linolenic acid concentrations exceeded the two parental values in 5 and 14 DH lines, respectively, suggesting that these two fatty acids had transgressive segregation. Therefore, the DH population can be utilized for the biosynthesis of unsaturated fatty acids in rapeseed and related genes. It can also be used as a breeding material for varieties with high oleic acid concentrations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.2
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• pp.95-110
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• 2022

This study was conducted to determine the extent to which climate change is expanding areas in which barley can be successfully cultivated. In 2019 and 2020, we collected data on areas that had requested certified seeds from the Korea Seed and Variety Service to determine potential cultivation areas. In addition, we surveyed the growth and yield of different types of barley in fields. Certified seeds of hulled and dehulled barley were requested by farmers across Korea from the Korea Seed and Variety Service in both years. Areas that were provided with certified seeds were considered potential barley cultivation areas. The varieties and use rates of certified seeds varied based on the barley type and region. For example, certified seeds of dehulled barley in 2019 and 2020 were not used in some areas, whereas in others, these seeds constituted 100% of the seeds sown for barley crops. In 2019 and 2020, the average sowing days in Korea were from October 17 to November 9 for dehulled barley, October 26 to November 13 for hulled barley, October 19 to November 5 for malting barley, and October 3 to November 1 for naked oats. Thus, the sowing days of the barley types varied depending on the area and year they were used. For example, in the case of hulled barley in Jeonnam, some farmers sowed until December 12. The yield per 10 a of barley cultivation was typically higher in the main production areas than in the cultivation limit areas. In extreme cases, harvest was impossible in some cultivation limited areas, such as Gangwon-do. Based on the current 20-year January minimum average temperature (JMAT) in Korea (2002-2021), climate change scenarios suggest that barley cultivation is feasible, provided that the minimum temperature in January is no lower than -10℃, -8℃, and -4℃ for hulled barley, dehulled barley, and for malting barley and naked oats, respectively. Additionally, cultivation of barley across South Korea seems feasible based on data on certified barley seeds by area. Although both JMAT and certified seed data suggest that barley cultivation across Korea is feasible, our survey results of barley growth and yield showed that harvest was impossible in certain cultivation areas, such as Gangwon-do. Therefore, climate change scenarios related to the cultivation limits of different barley types need to be re-estimated by factoring in survey data on the growth and yield of crops within those cultivation areas.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.2
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• pp.111-120
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• 2022

Improving flour quality is one of the major targets of wheat breeding programs. This study determined the optimum high-molecular-weight glutenin subunits (HMW-GS) to improve flour quality, and analyzed the correlation between agronomic and quality traits in Korea. A total of 180 wheat varieties, including 55 Korean and 125 foreign cultivars, carrying various Glu-1 alleles, were evaluated for their quality and agronomic traits. Results indicated that Glu-A1b, Glu-B1b, and Glu-D1f were the most prevailing alleles for each Glu-1 locus for Korean wheat cultivars. Korean wheat cultivars recorded shorter days to heading (DTH) and longer days to maturity (DTM) compared to foreign cultivars. In addition, an interaction effect was found between Glu-A1 and Glu-B1 alleles on several quality parameters. The combination of Glu-A1c and Glu-B1i showed a higher protein content, dry gluten content, and higher sodium dodecyl sulfate (SDS) sedimentation value than other Glu-A1×Glu-B1 combinations. Cultivars carrying Glu-A1a or Glu-A1b, Glu-B1i or Glu-B1al, and Glu-D1d for each Glu-1 locus exhibited a longer mixing time and stronger mixing tolerance. The DTM positively correlated with the protein content, gluten index and SDS sedimentation value. However, a negative correlation was observed between DTH and quality traits. Owing to the above results, this study suggests that an increase in the frequency of Glu-B1i or Glu-B1al, Glu-D1d coupled with a short DTH and long DTM could significantly improve wheat quality properties.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.3
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• pp.164-178
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• 2022

Uncertainties in weather forecasts would affect the reliability of yield prediction using crop models. The objective of this study was to compare uncertainty in crop yield prediction caused by the use of the weather forecast data. Daily weather data were produced at 10 km spatial resolution using W eather Research and Forecasting (W RF) model. The nearest neighbor method was used to downscale these data at the resolution of 5 km (W RF5K). Parameter-elevation Regressions on Independent Slopes Model (PRISM) was also applied to the WRF data to produce the weather data at the same resolution. W RF5K and PRISM data were used as inputs to the CROPGRO-SOYBEAN model to predict crop yield. The uncertainties of the gridded data were analyzed using cumulative growing degree days (CGDD) and cumulative solar radiation (CSRAD) during the soybean growing seasons for the crop of interest. The degree of agreement (DOA) statistics including structural similarity index were determined for the crop model outputs. Our results indicated that the DOA statistics for CGDD were correlated with that for the maturity dates predicted using WRF5K and PRISM data. Yield forecasts had small values of the DOA statistics when large spatial disagreement occured between maturity dates predicted using WRF5K and PRISM. These results suggest that the spatial uncertainties in temperature data would affect the reliability of the phenology and, as a result, yield predictions at a greater degree than those in solar radiation data. This merits further studies to assess the uncertainties of crop yield forecasts using a wide range of crop calendars.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.641-651
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• 2022

The biodiversity research of barley, a functional food, is proceeding to conserve germplasms and develop new cultivar of barley to improve its functional effects. In this study, with 25,104 barley germplasms in the National Agrobiodiversity Center, South Korea, the biodiversity index of species was much lower (1.17) than the origins (24.73) because of the presence of a biased species, Hordeum vulgare subsp. vulgare, but the species and origin of germplasms were significantly different with regard to genetic traits. In the clustering analysis based on genetic traits, we found that 97% barley germplasms could mostly be distributed between 1~7 clusters out of a total of 15 clusters; 'normal and uzu type', 'lodging', and 'loose smut' were commonly represented in the 1~7 clusters and some clusters showed specific differences in five genetic traits including 'growth habit'. In correlation of each genetic trait, the infection of 'barley yellow mosaic virus' was highly correlated to 'number of grains per spike'. '1000 grain weight' was weakly correlated with seven genetic traits including 'number of grains per spike'. Our analysis for barley's biodiversity can provide a useful guide to the species' phenotypes that need to be collected to conserve biodiversity and to breed new barley varieties.

• Korean Journal of Environment and Ecology
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• v.36 no.5
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• pp.445-459
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• 2022

In this study, we investigated current vegetation and assessed the health of vegetation through the KERVI (Korea Estuary Riparian Vegetation Index) to monitor vegetation changes near estuaries due to the opening of the Nakdong River estuary. As the first investigation of the long-term monitoring, six areas near the Nakdong River estuary were surveyed twice in July and October 2021, and vegetation monitoring and a survey of species composition and distribution density of aquatic, riparian, and land plants were carried out. The survey identified 262 taxa, 82 families, 192 genera, 196 species, 3 subspecies, 26 varieties, and 1 form of vascular plants in the surveyed area. The results of the vegetation health assessment through KREVI showed that sites 1 and 6 were rated "Very good" in both surveys, sites 2 and 4 were rated "Very good" in the first survey and then "Good"in the second survey, and site 3 and 5 were rated one grade higher in the second survey than the first survey. The assessment showed that the health grades of most species in the survey area were generally high. Most of the potential natural vegetation after the opening of estuary gates to create a brackish water area is expected to consist of reed (Phragmites communisTrin.) communities. The area of the willow (Salix koreensisAndersson) community adjacent to the water area may be somewhat narrower, but the community will be maintained. In the case of freshwater areas in inland areas with very low salinity, reeds (Phragmites communisTrin.) are expected to occupy most of them, and some communities such as amur silver-grass (Miscanthus sacchariflorusBenth.) and cattails (Typha orientalisC. Presl) are expected to be distributed. We suggest establishing measures such as estuary gate operation to create healthy brackish water regions through long-term monitoring.

• Korean Journal of Agricultural and Forest Meteorology
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• v.24 no.4
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• pp.256-266
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• 2022

Empirical models including the Angstrom-Prescott (AP) model have been used to estimate solar radiation at sites, which would support a wide use of crop models. The objective of this study was to estimate two sets of solar radiation estimates using the AP coefficients derived for climate zone (AP_Frere) and specific site (AP_Choi), respectively. The daily solar radiation was estimated at 18 sites in Korea where long-term measurements of solar radiation were available. In the present study, daily solar radiation and sunshine duration were collected for the period from 2012 to 2021. Daily weather data including maximum and minimum temperatures and rainfall were also obtained to prepare input data to a process-based crop model, CERES-Rice model included in Decision Support System for Agrotechnology Transfer (DSSAT). It was found that the daily estimates of solar radiation using the climate zone specific coefficient, SFrere, had significantly less error than those using site-specific coefficients S_Choi (p<0.05). The cumulative values of S_Frere for the period from march to September also had less error at 55% of study sites than those of S_Choi. Still, the use of S_Frere and S_Choi as inputs to the CERES-Rice model resulted in slight differences between the outcomes of crop growth simulations, which had no significant difference between these outputs. These results suggested that the AP coefficients for the temperate climate zone would be preferable for the estimation of solar radiation. This merits further evaluation studies to compare the AP model with other sophisticated approaches such as models based on satellite data.

• Korean Journal of Environment and Ecology
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• v.36 no.3
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• pp.220-256
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• 2022

The most important thing for conserving plant diversity in an area is to make an overall inventory of the plant species inhabiting the area. In particular, limestone areas are known for their high plant diversity and distribution of specific plants. Despite that, only a few have been designated as protected areas. This study investigated the vascular plants distributed in Goyangsan Mtn. and Munraesan Mtn., located in limestone areas of the central part of the Korean Peninsula. A field survey was conducted eight times from April to October 2021. As a result, we identified a total of 654 taxa comprising 113 families, 357 genera, 592 species, 15 subspecies, 44 varieties, and 3 formulas. They included four endangered wild plant species: Astilboides tabularis, Eleutherococcus senticosus, Cypripedium macranthos, and Epilobium hirsutum. Endemic plants in Korea were identified as 32 taxa. Floristic target plants were identified as 168 taxa, specifically 5 taxa of grade V, 41 taxa of grade IV, and 36 taxa of grade III. The red data plants included 2 taxa as "Endangered (EN)", 7 taxa as "Vulnerable (VU)", and 7 taxa as "Near threatened (NT)". A total of 41 taxa of naturalized plants were identified, and 4 of them were invasive alien plants. The surveyed vicinity of Goyangsan Mtn. and Munraesan Mtn. showed high plant diversity and contained core habitats for distribution of an endangered wild plant, Astilboides tabularis,in the limestone area. Moreover, both mountains contained a small population of Cotoneaster integerrimus. These findings confirm that the area has conservation values. Therefore, we propose to identify areas with high plant diversity and designate them as special protected areas.

• Journal of Environmental Science International
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• v.31 no.5
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• pp.413-422
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• 2022

To identify rice (Oryza sativa L.) cultivars suitable for Italian ryegrass (Lolium multiflorum Lam.)-rice double cropping systems, we investigated the yield and grain quality of four different midseason maturing rice cultivars ('Daebo', 'Haepum', 'Haiami', and 'Samdeog') and four midseason-to-late maturing rice cultivars ('Hyunpoom', 'Saeilmi', 'Saenuri', and 'Samkwang') in single rice cropping and Italian ryegrass-rice double cropping systems in paddy fields of Miryang, South Korea. We found that organic matter and available P₂O₅ content slightly decreased, whereas Na content increased, in the soil where Italian ryegrass was cultivated during winter compared to that in the soil that remained fallow during winter. The pH, electrical conductivity, organic matter, and contents of K⁺, Ca²⁺, Mg²⁺, and Na⁺ decreased, whereas the available P₂O₅ content slightly increased, in the soil where rice was harvested in both single and double cropping systems. However, compared to the optimum soil conditions for rice cultivation, available P₂O₅ and K⁺ content were high and Mg²⁺ content was low in both single and double cropping systems. At the heading stage, the culm length and leaf color slightly increased in most of the rice cultivars, whereas the panicle length and number slightly decreased, in the double cropped system. After harvesting, spikelet number and milled rice yield did not show a significant difference between single and double cropping systems. However, the ripened grain rate and weight per thousand grains increased slightly in the 'Saeilmi' and 'Samkwang' cultivars but remained either stable or slightly low in other cultivars in the double cropping system. The milled rice yield was high (> 500 kg/10a) in 'Daebo' and 'Haepum' among midseason maturing rice cultivars, and in 'Saeilmi' and 'Saenuri' among midseason-to-late maturing rice cultivars, in both single and double cropping systems. The head rice rate was high in midseason maturing rice cultivars in the double cropping systems, reaching > 70% in 'Haepum' and 'Haiami' cultivars, whereas it decreased in most midseason-to-late maturing rice cultivars (excluding 'Samkwang' cultivar), in double cropping systems. Particularly, it exceeded > 70% in the 'Saenuri' cultivar in both single and double cropping systems. The protein content in milled rice increased, whereas the amylose content either remained stable or slightly increased, in double cropping systems. The Toyo taste value decreased in all midseason-to-late maturing rice cultivars and slightly increased in the 'Daebo' and 'Haiami' cultivars among midseason maturing rice cultivars in double cropping systems. However, Toyo taste values in the 'Haepum', 'Haiami', and 'Saenuri' cultivars exceeding > 80% in both single and double cropping systems. Therefore, we recommend 'Haepum', 'Haiami', and 'Saenuri' cultivars as candidates for Italian ryegrass-rice double cropping systems due to high yield, head rice rate, and Toyo taste value.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.476-484
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• 2022

This study was conducted to investigate the effect of closed cultivation and open cultivation method and substrate type on the nutrient ion change pattern and growth of sweet pepper (Capsicum annuum L.) 'Scirocco' according to the reuse of drainage in hydroponics. The sowing, transplanting, and application of the closed and open cultivation method were carried out on August 19 and September 16, and October 21, 2021, respectively. As a result of the analysis of nutrients in the drainage, Na⁺ and Cl^- are representative ions that crops do not absorb properly, and as the growth progresses, they are accumulated in the closed method. In addition, since the content of NH₄-N in the drainage is significantly lower than that of NO₃-N, it is thought that NH₄-N is preferentially absorbed rather than NO₃-N due to the ion selectivity of sweet pepper. The growth and fruit characteristics of sweet pepper did not differ significantly between treatments according to the drainage reuse and the type of substrate. In conclusion, if you take care of poor fruiting due to the weakening of power after the middle period in hydroponic cultivation of sweet pepper according to the cultivation method of closed and open, and the substrate type of coir and rock wool, the difference between treatments is not large, so the sweet pepper can be produced by selecting the cultivation methods and substrate types suitable for the conditions of grower. However, as interest in environmental pollution has recently increased, it is judged that there is no need to worry about a decrease in quantity or quality, even if a closed cultivation method is adopted under the assumption that pathogen infection due to drainage reuse is well managed. It is expected that if coir is applied instead of rock wool, which causes a problem of disposal, it will further contribute to the reduction of environmental pollution.