• Korean Journal of Food Science and Technology
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• v.39 no.5
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• pp.580-583
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• 2007

Little is known about the genetic variability in lutein content for soybeans (Glycine max L.) cultivated in Korea. Therefore, the objective of this study was to screen high quality soybean cultivars for lutein content. The lutein contents of 23 recommended varieties, 56 sauce varieties, 44 sprout varieties, and 40 local varieties were determined by saponification followed by reversed phase high performance liquid chromatography (HPLC). We found that IT No. 153398 and 22789, originating from sauce and local varieties, respectively, contained significantly higher amounts of lutein as compared to the other 161 samples. Moreover, relevant quantitative differences were found among the samples, which had lutein contents ranging from 83 to $402{\mu}g/100g$ in the recommended varieties, from 39 to $778{\mu}g/100g$ in the sauce varieties, from 52 to $589{\mu}g/100g$ in the sprout varieties, and from 106 to $861{\mu}g/100 g$ in the local varieties, respectively. In summary, this study provides reliable lutein data for soybean cultivars that can be used for breeding studies and the development of functional foods.

• Journal of Mushroom
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• v.20 no.3
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• pp.107-112
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• 2022

This study was conducted to reduce the phenomenon of biased cultivation and to pursue the diversification of mushroom items. We focused on developing Sparassis latifolia varieties with a short cultivation period, stable pinheading, and a high yield. We have collected and determined the characteristics of genetic resources domestically and abroad since 2016. We bred the unique domestic variety, "Hanyeoul" and the results of this study are as follows. The optimal temperatures for mycelial growth and fruit body growth were 22~25℃ and 19~21℃, respectively. The color of the pileus was pale-yellowish white, which was similar to the color of the control variety, "Neoul". The number of days required for cultivation was 125 for bottle cultivation and 98~102 for bag cultivation, which was shorter than the number of days required for the control variety. Bottle cultivation took 39 days shorter for the new variety than the control variety. The fruiting body of the new variety was similar in size or larger than that of the control variety. The pinheading rate was more than 90% in bottle cultivation and bag cultivation, which was more stable than pinheading in the control variety. The yield was 79.1 g/bottle (1,100 cc) for bottle cultivation, which was 22.6% higher than the yield of the control variety, and 641.1~689.5 g/bag (2.6 kg) for bag cultivation, which 5.8~10.4% higher than yield of the control variety.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.130-134
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• 2011

Paprika (Capsicum annuum L.), a colored bell-type sweet pepper, is one of the most important money making vegetable crops in Korea. The cultivation area, total production, and exports of paprika are gradually getting increased, but the paprika cultivars used in Korea are all imported. It was well-known that the genic male sterility (GMS) is the main way to produce paprika hybrid seeds. However, it is little known that how many and what kinds of ms genes are used for breeding of paprika $F_1$ varieties. In this study, eight paprika cultivars ('Special', 'Debla', 'Plenty', 'Fiero', 'Boogie', 'Fiesta', 'Derby', and 'Minibell'), popularly cultivated in Korea and three different genic male sterile lines ('GMSP', 'GMS3', and 'GMSK') were used. For allelism test among the $F_1$ cultivars, half diallel crosses were performed. The result demonstrated that the most of the GMS in paprika cultivars except for 'Minibell' were same allele. To identify which GMS gene(s) were used for paprika $F_1$ cultivars, top crosses between previously known GMS lines and the $F_1$ cultivars were performed. As a result, we found that the $ms_k$ and the $ms_p$ genes were alleles for the GMS of 'Minibell' and for the other cultivars, respectively. We also confirmed that the GMS gene identification using GMSK-CAPS marker linked to the $ms_k$ gene and the PmsM1-CAPS marker linked to the $ms_p$ gene in $F_2$ progenies of 'Minibell' and 'Fiesta' and 'Derby' cultivars, respectively. In addition, we developed the PmsM2-CAPS marker for 'Plenty', 'Fiero', and 'Boogie' cultivars. We expect that these markers will be very useful for breeding new maternal (male sterile) line of paprika.

• 농업기술회보
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• v.49 no.4
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• pp.30-31
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• 2012

국내에 재배되는 복숭아의 품종은 다양하다. 경기도 이전지역에서만 보면, 7월 중순부터 조생종인 '용궁백도', '미백조생'이 출하되기 시작하여 본격적으로 8월 중순에 중생종인 '미백도', 8월 하순부터 만생종인 '천중도백도', 9월 초 중순부터 극만생종인 '장호원황도'가 수확된다. 그러나 본격적 복숭아 수확기인 8월 중순부터 미백도~천중도백도 사이의 단경기를 극복할 수 있는 우량품종이 절실히 요구됐다. 또한 대부분의 복숭아 품종들이 보구력이 약해서 수확, 운반 및 저장에 많은 어려움을 느끼고 있었다. 이에 따라 이재권씨는 미백도 나무에서 접목변이지로 미백도와 다른 모양의 복숭아를 발견하고 기술개발에 참여하게 되었다.

• 농업기술회보
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• v.50 no.3
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• pp.31-31
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• 2013

최근 국내에 발생해 큰 문제를 일으키는 토마토황화잎말림바이러스(TYLCV)는 매개충인 담배가루이와 함께 토마토의 안정생산을 위협하는 치명적인 바이러스다. 병이 발생하면 악제나 다른 방법으로 방제가 어려우므로 내병성 풍종, 특히 TYLCV 저항성 유전자 Ty-1,2,3(부분우성)의 한 개체 내 집적을 통해 저항성을 높인 품종의 육성이 시급하다. 이런 목적으로 국립원예특작과학원에서는 TYLCV에 저항성 품종인 '티와이썬'을 개발하였다.

• Proceedings of the Korean Society of Grassland Science Conference
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• 1999.06a
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• pp.96.1-96
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• 1999

본 연구는 주요 목초에서 엔도파이트가 생성하는 물질을 구명하고, 안정수준의 물질을 생산하는 우수종을 분리 접종하여 환경저항성 식물을 개발하려고 수행하였다. 공시재료는 톨 페스큐 5 품종을 선발 공시하였으며, 엔도파이트 비율과 품종의 생육특성, 수량과 사료가치를 비교하였다. 주요 연구결과로는 톨 페스큐 품종 중 엔도파이트 비율이 29%인 Barvetia가 초장과 엽폭이 크고, 녹색도가 우수하였다.(중략)

• Horticultural Science & Technology
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• v.30 no.3
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• pp.278-285
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• 2012

Cymbidium goeringii is one of the most important and popular species in the orchid family in north-east Asia. In the present study, we prepared multiplex PCR system, and used it for the genotyping of eight simple sequence repeats (SSR) markers (CG409, CG415, CG709, CG722, CG787, CG1023, CG1210, and CG1281) in subject with 40 samples of cultivated varieties. All the analyzed samples showed different combined genotypes. The average combined power of discrimination was very high value of $7.14{\times}10^{-10}$, and observed heterozygosity (Ho = 0.466) was similar with two wild populations of C. goeringii, which may indicate no or little occurrence of genetic change after collection from wild habitats. The present study also developed a two-dimensional barcode to express information of genotype results of eight SSR markers (SSR DNA ID). The discrimination power of DNA ID between two individuals will be statistically more than 99.999999%. The SSR DNA ID and two-dimensional barcode may be very usefully applied for the discrimination and maintence of cultivars of C. goeringii.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.159-164
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• 2014

For development of a method for differentiation of Pleurotus eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotyping results. PCR using each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in the range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pairgroup method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into two clusters. Cluster I and II were comprised of four and eight cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivars and strains with 21 alleles and a PIC value of 0.9090. These results might be useful in providing an efficient method for the identification of P. eryngii cultivars with separate PCR reactions.

• Journal of Korean Society of Forest Science
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• v.108 no.3
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• pp.302-310
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• 2019

Precise and fast identification of crop cultivars is essential for efficient breeding and plant breeders' rights. Traditional methods for identification of jujube cultivars are based on the evaluation of morphological characteristics. However, due to time constraints and environmental influences, it is difficult to distinguish cultivars using only morphological traits. In this study, we cloned fragments from improved inter simple sequence repeats (ISSR) analysis, and developed stably diagnostic sequence-characterized amplified region (SCAR) markers. The specific ISSR bands of jujube cultivars from Dalizao and Boeundaechu were purified, cloned, and sequenced. As a result, four clones labeled 827Dalizao550, 827Boeun750, 846Boeun700, and 847Dalizao850 were identified. In order to investigate whether they were specific for the jujube cultivar, four pairs of SCAR primers were then designed and polymerase chain reaction (PCR) amplifications were conducted to analyze 32 samples, including jujube and sour jujube. In the PCR amplification of the 827Dalizao550 SCAR marker, the specific bands with 550 bp were amplified in six samples (Dalizao, Sandonglizao, Dongzao, Yuanlin No. 2, Suanzao 2, Suanzao 4), but unexpected bands (490 bp) were amplified in the others. Moreover, in the PCR amplification of the 847Dalizao850 SCAR marker, the specific bands with 850 bp were found in three samples (Dalizao, Sandonglizao, and Dongzao) and 900 bp unexpected bands were amplified in five samples (Pozao, Suanzao 1, Suanzao 2, Suanzao 3, Suanzao 4). These results showed that newly developed markers could be useful as a fast and reliable tool to identify jujube cultivars. However, further identification of polymorphic information and the development of SCAR markers are required for the identification of more diverse cultivars.

• Landscaping Tree
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• s.127
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• pp.54-57
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• 2012

우리나라도 국제화 시대에 대비한 조경수 생산기술개발이 어떠한 방향으로 이루어질 것인가? 조경수를 연구하고 있는 본인에게도 어려운 질문 중에 하나이다. 무엇보다도 국내의 조경수 생산기술 및 유통체계가 구축되지 않아 총체적인 문제점을 지니고 있기 때문이다. 이에 따라 현시점에서 국제화 시대에 대비하는 것이 우선적으로 국가나 지자체에서 작목반을 구성하여 그 지역에 맞는 새로운 품종육성으로 계속해서 생산할 수 있는 품종과 국제적으로 정보를 통하여 수출입을 할 수 있어야 한다. 그리고 홍보 및 전문가의 컨설팅으로 재배관리 및 수종선택이 필요함과 동시 모든 것이 기계화, 규격화로 이루어지는 시스템이 중요하다.