• Title/Summary/Keyword: 폴리유산

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Total Polyphenol Contents, Flavonoid Contents, and Antioxidant Activity of Roasted-flaxseed Extracts Based on Lactic-acid Bacteria Fermentation (유산균 발효에 따른 볶은 아마씨 추출물의 폴리페놀, 플라보노이드 함량 및 항산화 활성)

  • Park, Ye-Eun;Kim, Byung-Hyuk;Yoon, Yeo-Cho;Kim, Jung-Kyu;Lee, Jun-Hyeong;Kwon, Gi-Seok;Hwang, Hak Soo;Lee, Jung-Bok
    • Journal of Life Science
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    • v.28 no.5
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    • pp.547-554
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    • 2018
  • Flaxseed (Linum usitatissimum L.), also called linseed and one of the raw materials for making linen, is rich in omega-3 fatty acids, vegetable estrogen, ${\alpha}$-linolenic acid, and dietary fiber. Studies on flaxseed have reported various additional effects, such as the inhibition of cholesterol, blood clotting, and tumor growth. In this study, we investigated the functional components of flaxseed fermented with lactic-acid bacteria. Lactic-acid bacteria was inoculated into heat-treated (roasted) flaxseed and fermented at $37^{\circ}C$ for 72 hr. The fermented flaxseed was extracted with 70% ethanol and the antioxidant effect of the fermented extracts according to the lactic-acid bacteria was analyzed. It was confirmed that the total polyphenol contents had expanded by about 1.5-8 times, and the total flavonoid contents had increased around 1.2 times in the case of fermented flaxseed with lactic-acid bacteria compared to non-fermented flaxseed (NFFS). DPPH radical scavenging and superoxide dismutase-like activities had increased around 5.6 and 2.3 times, respectively, in the fermented flaxseed compared to the NFFS at 100 ppm concentration. The study concluded that fermentation of flaxseed with lactic-acid bacteria is possible and that it is effective to increase the antioxidant effects of flaxseed. These results can be applied to the development of improved foods and cosmetic materials.

Antioxidative Effects of Mushroom Extract and Fermented Milk Containing Its Extract on in vivo and in vitro Lipid Peroxidation (버섯 추출물과 이를 함유한 유산균 발효유가 in vivo 및 vitro 과산화지질에 미치는 영향)

  • 차재영;전병삼;박정원;신갑균;김범규;배동원;유지현;전방실;조영수
    • Journal of Life Science
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    • v.14 no.3
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    • pp.514-520
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    • 2004
  • The antioxidative effects of fermented milk, mushroom extract and fermented milk containing its extract (Lentinus edodes, Ganoderna lucidum, and Pleurotus ostreatus) on the lipid peroxidation in the tissues of female Sprague-Dawley rats and on the DPPH ($\alpha,\alpha$' -diphenyl-$\beta$-picrylhydrazyl) radical donating ability were studied. The total concentrations of polyphenolic compound in Lentinus edodes, Ganoderma lucidum and Pleurotus ostreatus were 0.34, 0.20 and 0.34%, respectively. The DPPH donating abilities of mushroom extract, fermented milk, fermented milk containing its extract and BHT (butylated hydorxytoluene) as standard were 33.9, 34.9, 51.9 and 95.6%, respectively. Experimental diet groups were divided into five groups: the normal diet (ND), the cholesterol diet (CD), and cholesterol + fermented milk diet (CDFM), cholesterol + mushroom extract diet (CDME) and cholesterol + fermented milk containing mushroom extract diet (CDFMME). The concentrations of lipid peroxide in liver and its microsome were significantly lower in both CDFM and CDFMME groups than in the other groups. The kidney concentration of lipid peroxide was significantly higher in the CD group than in the ND group, but this rise were significantly decreased in the CDFM and CDFMME groups. Meanwhile, the concentrations of heart and spleen and their fractions were not significantly different among dietary groups. This study was suggested that the fermented milk diet containing mushroom extract effectively reduced the lipid peroxidation in liver and kidney of cholesterol-fed female rats.

A Study on the Effect of CNT on Crystallization Kinetics and Hydrolytic Degradation of PKA/CNT Composite (PLA/CNT 복합재료의 결정화 특성 및 가수분해에 미치는 CNT 영향에 대한 연구)

  • Li, Mei-Xian;Kim, Sung-Ha;Kim, Si-Hwan;Park, Jong-Kyoo;Lee, Woo-Il
    • Composites Research
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    • v.24 no.4
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    • pp.5-10
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    • 2011
  • As environmental pollution getting worse, biodegradable materials have been drawn more attention than ever. In this study, polylactic acid (PLA)/carbon nanotubc (CNT) nanocomposites were manufactured via extrusion molding and injection molding, In order to change the crystallinity, annealing treatment was done for different time span, Crystallization kinetics of PLA was analyzed by differential scanning calorimeter (DSC), and it was confirmed that a proper amount of CNT can increase the crystallization rate of PLA. In addition, the presence of CNT significantly accelerates the hydrolytic degradation rate of PLA, however, it decreases with the increase of crystallinity. The reason is that degradation may occur in the PLA/CNT interface easily, and the molecular structure of the composite becomes dense with the increase of crystallinity.

Three-dimensional Digital Restoration and Surface Depth Modeling for Shape Analysis of Stone Cultural Heritage: Haeundae Stone Inscription (석조문화유산의 형상분석을 위한 3차원 디지털복원과 표면심도 모델링:해운대 석각을 중심으로)

  • Jo, Young-Hoon;Lee, Chan-Hee
    • Journal of Conservation Science
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    • v.28 no.1
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    • pp.87-94
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    • 2012
  • This study was focused on digital restoration and surface depth modeling applying the three-dimensional laser scanning system of the Haeundae Stone Inscription. Firstly, the three-dimensional digital restoration carried out acquiring of point cloud using wide range and precision scanner, thereafter registering, merging, filtering, polygon mesh and surveyed map drawing. In particular, stroke of letters, inscribed depth and definition appearing the precision scanning polygon was outstanding compared with ones of the wide range polygon. The surface depth modeling completed through separation from polygon, establishment of datum axis, selection of datum point, contour mapping and polygon merging. Also, relative inscribed depth (5~17mm) and outline by the depth modeling was well-defined compared with photograph and polygon image of the inscription stone. The digital restoration technology merging wide range and precision scanning restored the total and detailed shape of the Stone Inscription quickly and accurately. In addition, the surface depth modeling visibly showed unclear parts from naked eye and photograph. In the future, various deteriorations and surrounding environment change of the Stone Inscription will be numerically analyze by periodic monitoring.

Effect of Melt-mixing Conditions on Fracture Properties of Bioabsorbable HA/PLLA Composite Materials (생체흡수성 HA/PLLA 복합재료의 용융혼련조건이 파괴특성에 미치는 영향)

  • Park, Sang-Dae;Lee, Deok-Bo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.31 no.7 s.262
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    • pp.732-738
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    • 2007
  • Effects of melt-mixing conditions on fracture properties of hydroxyapatite filled bioabsorbable poly(L-lactic acid)(HA/PLLA) composites was investigated by measuring the firacture toughness value of HA/PLLA composites prepared under different mixing time and rotor speed. The fracture surface morphology was also examined by profile measurement and scanning electron microscopies. It was found that the fracture toughness of HA/PLLA composites decreases due to decrease of ductile deformation of PLLA matrix and debonding of interfaces with increase of the rotor speed and mixing time. Effect of mixing process on neat PLLA was also assessed, and it was found that the fracture toughness of PLLA decreases due to disappearance of multiple craze formation and increase of defects. Such thermal and shear-stress degradation were found to be the primary mechanisms of the degradation of HA/PLLA composites during melt-mixing process.

Biological Activities of Solid-fermentation Garlic with Lactic Acid Bacteria (Lactic acid bacteria를 이용한 마늘 고체 발효에 따른 생리활성)

  • Lee, Jung-Bok;Joo, Woo-Hong;Kwon, Gi-Seok
    • Journal of Life Science
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    • v.26 no.4
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    • pp.446-452
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    • 2016
  • Garlic (Allium sativum L.) and its extracts have been used in a wide range of applications, including as folk medicines in many Asian countries. This traditional herb has several functional properties and strong biological activities, making it useful as a functional food material. This study investigated the biological activities of solid-garlic after fermentation by lactic acid bacteria. Several solid-garlic fermentation strains of lactic acid bacteria were isolated from Korean traditional fermented food or obtained from the Korean Collection for Type Cultures. Lactic acid bacteria showed selective growth in garlic extracts on MRS media. Fermentation of solid garlic (heated 121℃, 15 min or not heated) by lactic acid bacteria gave total polyphenol and flavonoid contents of 731.0-845.2 g/g and 92.68-413.58 g/g, respectively. The DPPH scavenging activities and SOD like activities were measured as 7,584% and 9499%, respectively. These activities were relatively higher than a positive control, vitamin C. Measurement of antidiabetic activity using α-glucosidase inhibition assay showed that solid garlic fermented with lactic acid bacteria gave a higher activity than the control, acarbose. Fermentation of solid garlic with lactic acid bacteria may therefore help to alleviate adverse biological activities, as well as provide functional food materials.

Improvement of Antioxidative Activity by Enzyme Treatment and Lactic Acid Bacteria Cultivation in Black Garlic (효소 처리와 유산균 배양에 의한 흑마늘의 항산화 활성 향상)

  • Chae, Hee-Jeong;Park, Dong-Il;Lee, Sung-Chul;Oh, Chul-Hwan;Oh, Nam-Soon;Kim, Dong-Chung;Won, Sun-Im;In, Man-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.660-664
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    • 2011
  • We investigated the improvement of the antioxidative activity of black garlic with enzymatic treatment and lactic acid bacteria cultivation conditions. Celluclast, a commercially-available polysaccharide hydrolyase, was selected to obtain high total polyphenol content in a black garlic suspension. A lactic acid bacterial strain showing fast growth and high acid production in a black garlic suspension was isolated from Kimchi. This strain was identified as Lactobacillus pentosus 310-7. Enzymatically hydrolyzed black garlic was fermented using the L. pentosus 310-7 strain at $30^{\circ}C$ for 15 hr. The pH and titratable acidity achieved were 4.24 and 0.35%, respectively, after 15 hr fermentation. The viable cell population of L. pentosus 310-7 slowly increased to 7.54 log CFU/g. The polyphenolic compound content, known antioxidants, in black garlic was enhanced with Celluclast treatment and L. pentosus 310-7 cultivation. Total polyphenolic compounds were increased to approximately 60% of the initial concentration, and electron donating ability was also improved, from 39.8 to 65.9%.

Antioxidant Activities of Protaetia brevitarsis Larvae Fermented by Lactobacillus acidophilus (유산균 발효가 흰점박이꽃무지(Protaetia brevitarsis) 유충의 항산화활성에 미치는 영향)

  • Min Jeong, Park;Soo Jeong, Cho
    • Journal of Life Science
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    • v.32 no.11
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    • pp.890-898
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    • 2022
  • This study was carried out to evaluate the effect of fermentation by Lactobacillus acidophilus on the antioxidant activity of Protaetia brevitarsis larvae fed with mushroom substrates (king oyster mushroom). The total polyphenol content of the P. brevitarsis larvae extracts (PLEs) (93.33±0.98 mg GAEs/extract g) was higher than that of the fermented P. brevitarsis larvae extracts (FPLEs) (65.02±1.32 mg GAEs/extract g). The flavonoid contents of the PLEs and FPLEs were 18.3±1.57 QEs mg/extract g and 17.69±0.95 QEs mg/extract g, respectively. The DPPH radical scavenging activity showed no significant difference between the PLEs and FPLEs at a concentration of 2-4 mg/ml. However, at a concentration of 8 mg/ml or more, the DPPH radical scavenging activity of the FPLEs was higher than that of the PLEs. The reducing power of the FPLEs was also higher than that of the PLEs, and more than twice as high at a concentration of 1.6 mg/ml or more. The ORAC value of the FPLEs (79.22±0.72 μM TEs/extract g) was higher than that of the PLEs (74.34±0.37 μMTEs/extract g). A WST-1 assay of the RAW 264.7 cells indicated that the PLEs and FPLEs showed no cytotoxicity.

Effects of Sclerophyllous Plant Leaves Addition on Fermentative and Sensory Characteristics of Kimchi (경엽식물 잎의 첨가가 김치의 발효 및 관능특성에 미치는 효과)

  • Park, Dong-Ill;Choi, A-Reum;Woo, Hye-Jin;Rhee, Seong-Kap;Chae, Hee-Jeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.4
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    • pp.580-586
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    • 2010
  • The effects of persimmon, mulberry and bamboo leaves addition on the fermentative and sensory characteristics of kimchi were investigated. Total polyphenol content, DPPH radical scavenging activity and lactic acid bacteria growth inhibition of EtOH extract from persimmon leaves were significantly higher than those from mulberry and bamboo leaves. From the sensory evaluation of kimchi added with three chopped plant leaves, kimchi added with chopped persimmon leaves gave the highest point in color, flavor, texture and overall preference significantly (p<0.05). When the chopped persimmon leaves were added at a ratio of 0.3% based on cabbage weight, the changes of pH and total acidity (TA) during the storage for 14 days were significantly lowered, compared to general kimchi (p<0.05). Total polyphenol content and DPPH radical scavenging activity of kimchi supplemented with chopped persimmon leaves at 0.3% also significantly increased after storage for 14 days (p<0.05), and this kimchi provided higher total polyphenol content and DPPH radical scavenging activity than general kimchi. These results suggest that the addition of perisimmon leaves have significant influences on the fermentation and sensory characteristics of kimchi.