• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.438-443
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• 1999

Germination and growth of Laminaria japonica microscopic stages were investigated under crossed gradients of temperatures and irradiances, and the results related to the seasonal temperature regime in the southeastern coast of Korea. Germination rates of $70\~86\%$ were observed in the temperature range of $5\~20^{\circ}C$, however, at $25^{\circ}C$ no germination of meiospores was observed. The primary factor affecting germination rates at the temperature range of $5\~20^{\circ}C$ was irradiance: germination was significantly reduced at $150 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. Vegetative cell production of female gametophytes was highest at $20^{\circ}C$, but plants were not fertile at the temperature. In the temperature range of $5\~15^{\circ}C$, higher irradiance caused females to reduce cell production, but increased fertility. Cell production was also low at lower temperatures with increased fertility rates. Optimal growth temperature for microsporophytes was $10^{\circ}C$ and their growth rates were light-saturated at $70 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. We have concluded that meiospores released before July could develope to the young sporophytic stage in the southeastern coast of Korea which is off the southern limit of its geographical distribution. However, limiting factor in the development of natural sporophytic population in this region would be the upper temperature limit for the survival of young sporophytes, as water temperature at this area frequently exceeds $25^{\circ}C$ during the summer period.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.131-141
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• 2017

This study was conducted to investigate in vitro mass propagation methods suitable for each growth stage of A. aristata (G. Forst.) Tindale, from spore germination to sporophyte formation. Among spores germinated in $1/8-1{\times}MS$ medium and Knop medium, Knop medium yielded the highest germination percentage (87.1%). We cultured prothalli obtained from germinating spores for 8 weeks on media with different concentrations of sucrose and active carbon, as well as different concentrations and ratios of nitrogen, to select a suitable growth medium. A. aristata (G. Forst.) Tindale prothalli grew most actively in MS medium with 3% sucrose and 20 : 40 mM of $NH_4Cl$ and $KNO_3$ (total concentration of 60 mM). We investigated sporophyte formation according to soil type, finding that bedding soil mixed with perlite at a 2 : 1(v / v) ratio yielded the highest number of sporophytes per pot ($73.8/7.5{\times}7.5cm\;pot$). By contrast, when peat moss was used alone or mixed with other substrates, prothallus development and sporophyte formation were suppressed. Therefore, the most effective propagation method for A. aristata (G. Forst.) Tindale is to grow prothalli in MS medium and to induce sporophyte formation in a mixture of bedding soil and perlite (v / v = 2 : 1).

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.119-126
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• 2019

This study was conducted to investigate the optimal culture method for gametophyte and sporophyte propagation in Coniogramme japonica (Thunb.) Diels, which can be used in various fields. The propagation of prothallus were cultured in 1/4 - 1 Murashige and Skoog medium and Knop medium for 10 weeks. The results indicated that the fresh weight of prothallus was the highest (14.5 g) in 1MS medium. Subsequently, various concentrations of sucrose, activated charcoal and nitrogen source were also added to 1MS medium and cultured for 8 weeks. The results provided that the sucrose concentration was 3% and the fresh weight of prothallus was the highest 10.8 g. According to the concentration in the range of 8.8 ~ 10.8 g, in the case of activated charcoal, the four treatments showed no significant difference. The nitrogen source was added at a concentration of 30, 60 and 120 mM with the ratio of ${NH_4}^+:{NO_3}^-$ being 1 : 2. As a result, the fresh weight of all treatments increased to similar level but there was no significant difference. We investigated sporophyte formation according to soil type and the highest number of sporophytes at 228.0 was formed in soils mixed with horticultural substrate and decomposed granite at 2 : 1 (v : v). On the other hand, sporophyte was not formed in soils containing peatmoss except for the one with peatmoss and decomposed granite at 2 : 1 (v : v).

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.40-40
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• 2018

비고사리과(Lindsaeaceae) 버들일엽[Laxqgramme saliciblia (Makino) Makino]은 잎이 녹색의 단엽으로 버들처럼 보여 관상가치가 높아 조경소재로 이용되는 착생식물이다. 또한 식물구계학적 특정식물종 V급, 적색자료집 VU급, 국외반출 승인대상종에 수록된 식물로 생태학적 보전이 요구되며, 개체수도 적은 편으로 알려져 있다. 본 연구는 보전이 요구되는 버들일엽의 전엽체 증식 및 포자체 형성을 위한 기내 외 번식조건을 구명하고자 수행되었다. 서귀포시 상효동 일대에서 식물을 획득하였으며, 기내에서 포자를 발아시켜 획득한 전엽체를 8주 간격으로 계대배양하면서 실험재료를 확보하였다. 전엽체 증식에 적합한 배양배지를 선발하고자, 전엽체 300mg을 다진 후 배지종류와 농도를 달리한 배지(Knop, 1/4, 1/2 및 1MS)에 8주간 배양하였다. 배양실은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구의 결과, 1/2MS배지에서 전엽체의 생체중이 5.8g으로 가장 많이 증가하였으며, 형태형성의 발달도 주걱형의 전엽체로 정상적으로 발달하였다. 기외 포자체 형성에 적합한 토양조건을 확인하고자, 인공토양(원예상토, 피트모스, 펄라이트 및 마사토)의 비율을 달리하여 5종류의 혼합토양을 조성하였다. 준비된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하고, 전엽체 1g을 10초간 분쇄한 다음 토양표면에 분주하여 16주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 연구의 결과, 원예상토가 많은 비율로 혼합된 처리에서 포자체의 형성이 확인되었다. 원예상토와 펄라이트를 2:1(v:v)로 혼용한 토양, 원예상토와 마사토를 2:1(v:v)로 혼용한 토양, 원예상토 단용한 토양에서 각 28.5, 23.0, 22.3개로 조사되었다.

• Journal of Ginseng Research
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• v.24 no.2
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• pp.53-57
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• 2000

Chlamydospores were isolated from hyphae of Cylindrocanon destmctans by homogenization and/or ultrasonic treatment. Rate of the isolated chlamydospores by the homogenization with glass tissue grinder were 9.8% of all total chlamydospores formed in the culture of C. destructans. The length of mycelial fragments after the homogenization was about 400㎛ They were, however, formed in clusters of the chlamydospores and the mycelia The rate of the isolated chlamydospores from additional ultrasonic treatment after the homogenization of the mycelia were 74.3%. The length of mycelial fragments with the ultrasonic treatment was about 20 fm and chlamydospores seemed to be isolated from the mycelial mats and dispersed evenly in the culture. The numbers of chlamydospore in a catena were 1 to 8 cells after the homogenization on potato dextrose agar (PDA). Meanwhile the numbers of them after added ultrasonic treatment were 1 to 4 cells. Germination percentages of the isolated chlamydospores from the ultrasonic treatment were 46.8% after incubation of 2 days on PDA at 20。C and 60.7% after incubation of 13 days at 5。C, respectively. Germination rate of chlamydospores to the total chlamydospores produced by the ultrasonic treatment was 55.8%. However, it was increased to 74% when it was measured in the germinated catenae to the total catenae.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.269-270
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• 2000

홍조 지누아리류(Grateloupia spp.)는 독특한 향과 감촉을 가진 유용 해조류로서 강원도 지역에는 옛부터 식용으로 널리 이용되어 왔다. 그러나 산업적 생산량이 자연상태의 생물량에 한정되어 있어서 지역적 한계를 벗어나 그 우수한 풍미를 일반화시키지 못하고 있다. 본 연구에서는 강원도 연안에서 채집한 지누아리사촌(Grateloupia acuminata)의 사분포자체를 조직배양하여 재생체를 유도하고, 이로부터 사분포자를 얻어 배양조건에 따른 포자의 발아와 생장 경향을 파악하였다. 양식에 사용된 종묘는 사분포자로부터 얻은 배우체의 유엽으로서 이의 바다양성을 통하여 대량 양식의 가능성을 파악하고자 하였다. (중략)

• Journal of Life Science
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• v.13 no.2
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• pp.184-190
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• 2003

Myxococcus xanthus is a Gram negative, rod-shaped, soil bacterium that displays a social behaviors, and multicellular development upon nutrient deprivation. The csgA gene encoding a cell surface protein is essential for developmental behaviors including rippling, aggregation, fruiting body formation and sporulation. csgA mutants show normal vegetative growth, but lack all these developmental phenotypes. Expression of the CsgA (C-signal) specific genes are eliminated or dramatically reduced in csgA mutants. In order to identify components of C-signal transduction pathway, second site mutations were introduced into csgA mutants and were identified which can fully or partially restore development of csgA mutants (Rhie, H. G. et. al. 1989. J. Bacteriol. 171, 3268-3276). One of such csgA suppressor mutations, socD500 restores only sporulation to csgA mutants at 15$^{\circ}C$. The socD500 mutaion however eliminates the three basic developmental requirements, starvation, high cell density and a solid surface. Only sporulation, not accompanied with fruiting body formation is induced simply by shifting the temperature of vegetatively growing cells from $32^{\circ}C$ to $15^{\circ}C$. Spores induced by socD500 mutation is not as thick as that of wild-type fruiting body. In socD500 genetic background, two of ten C-signal dependent genes, $\Omega$DK4506 and $\Omega$DK4406 are more highly expressed in growing cells at $15^{\circ}C$. These results indicate that the socD500 mutation may be partly involved in the regulation of expression of two C-signal dependent genes and genes for sporulation in this transduction pathway.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.242-246
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• 2002

Growth and tetraspore release pattern of lithophyllum yessoense (Rhodophyta, Corallinaceae) were investigated from March 2000 to July 2001. Pinkish tetraspores were 40.2 $\pm$ 0.4 $\mu$m in diameter. After release, tetraspores attached on substrate shortly. Culture conditions were five temperatures (5, 10, 15, 20, 25^{\circ}C), four irradiances (0, 20, 50, 100 $\mu$mol${\cdot}m^{-2}$${\cdot}s^{-1}$) and six salinities (0, 9, 17, 25, 34, 43 ppt). Maximum growth of gametophyte was occurred at $20^{\circ}C$, $20{\mu}mol\;m^{-2}$${\cdot}s^{-1}$, 16: 8h (L:D) and 34 put. Maximum relative growth rate was 0.1232 at $20^{\circ}C$. The amount of tetraspore release showed maximum at September as 266 cells per crust area ($cm^2$), and tetraspores did not release from January to March.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.38-40
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• 2005

• The Korean Journal of Mycology
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• v.10 no.4
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• pp.187-192
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• 1982

The effects of amino acids on mycerial growth and sporulation of the decreased and increased pathogenicity variants of potato dry rot Fusarium roseum ‘Sambucinum' were as following. The increased pathogenicity variants Ic52 and Ic116 showed the trend of greater mycerial growth, marking up to 67mg on leucine, than the decreased pathogenicity variants Dc14 and Dc91, and produced markedly low conidia below $25{\times}10^{4}/ml$ or no sporulation. On methionine all the variants and the wild type showed the lowest growth recording 14mg to 20mg with no sporulation. Cystine similarly as glysine supported the poor growth 30mg to 19mg of the variants Dc14 and Dc91, respectively, and did not support the sporulation. The former amino acid like asparagine supported the growth of the variants Ic52 and Ic1l6 marking 47mg and 36mg, respectively. On all the eleven amino acids the variant Dc14 showed the trend of more mycerial growth and sporulation than the variant Dc91 which marked the exceptionally high sporulation $195{\times}10^{4}/ml$ on glutamic acid.