• The Korean Journal of Mycology
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• v.14 no.1
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• pp.71-78
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• 1986

Of the 1,200 single spore isolates of Alternaria mali causing apple leaf spot and fruit decay, the mycelial colonies showed 76, 96 and 15% growth at 100,000 ${\mu}g/ml$ of captafol, chlorothalonil and folpet, respectively, and five and three percent growth at 10,000 ${\mu}g/ml$ of iprodione and polyoxin. These isolates showed cross-tolerance of mycelial growth, spore formation, or spore germination to the above five fungicides plus captan, garbenda, and thiophanate methyl. Captafol, mancozeb, polydong, and propineb did not show any cross-tolerance of spore germination since no spore was germinated at 100 ${\mu}g/ml$ which was much lower than the concentration used by apple growers. Benomyl, fenarimol, oxidong, and triademefon showed medium degree of cross-tolerance of the fungal growth, sporulation, and spore germination.

• The Korean Journal of Mycology
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• v.16 no.1
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• pp.41-48
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• 1988

To investigate germination characteristics of soils on Fusarium species under different soil conditions, this study was carried out to test spore germination of 4 Fusarium species. Among 102 soil samples, spore germinations of Fusarium solani f. sp. pisi, F. oxysporum f. sp. cucumerium, F. oxysporum f. sp. raphani and F. coccophilum were under 50% in 98, 85, 82 and 83 soil samples, respectively. The highest spore germination of F. solani f. sp. pisi, F. oxysporum f. sp. cucumerium and F. coccophilum was obtained in 21 soil samples of pH 5.1-6.0, whereas F. oxysporum f. sp. raphani was in 33 soil samples of pH 4.1-5.0. The highest spore germination of F. solani f. sp. pisi, F. coccophilum and F. oxysporum f. sp. cucumerium was obtained in 4 soil samples holding soil moisture content of 41-60%, while F. oxysporum f. sp. raphani was in 22 soil samples of 21-30%. F. oxysporum f. sp. raphani only showed a direct correlation(r=0.29) between spore germination and soil moisture content. On the basis of each spore germination in cultivated and non-cultivated soils, there was significant difference(p=0.01) in 4 Fusarium species. F. solani f. sp. pisi only indicated significant difference(p=0.0l) between two contrasting-soils, cultivated and non-cultivated soils. The numbers of microbial population were higher in soils suppressive to spore germination than soils conducive to spore germination.

• Korean Journal Plant Pathology
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• v.12 no.2
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• pp.245-250
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• 1996

식물병원균 Botrytis cinerea와 Fusarium oxysporum에 대한 생물학적 방제의 기초자료를 얻기 위하여 살충성 진균 Beauveria bassiana의 식물병원균에 대한 균사성장저해, 포자발아 억제, 균사와 포자의 형태변화 등의 효과를 살펴보았다. 평판배지 상에서 두 식물병원균의 균사생장이 저해되었으며, 저해효과는 배지종류에 따라 달랐는데 B. cinerea의 경우 PDA배지에서 가장 크게 저해되었으며 F. oxysporum의 경우에는 TSA배지에서 가장 크게 저해되었다. B. bassiana는 $25^{\circ}C$에서 6일간 배양했을 때 F. oxysporum에 대한 항균력이 가장 높았으며 동시에 최대의 균체량을 생산하였다. 또한 B. bassiana의 배양여액을 식물병원균에 30% 농도로 첨가하여 배양했을 때 식물병원균의 포자 발아율은 B. cinerea, F. oxysporum에서 각각 30%(control: 88.2%), 10.0%(control: 78.6%)로 낮아졌으며 발아 개시 시간도 4~8시간 지연되었다. 현미경을 통한 미세구조관찰에서는 10%의 B. bassiana배양여액을 첨가했을 때 F. oxysporum의 포자 크기가 1/2~1/3으로 줄어들었으며 균사와 격막의 형태도 비정상적으로 변하여 균사외막과 격막-격막 사이의 구분이 불명확해졌다.

• Journal of Bio-Environment Control
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• v.21 no.2
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• pp.134-139
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• 2012

Inhibition effects on spore germination and mycelia growth for gray mold (Botrytis cinerea) were investigated in vitro using environment-friendly agricultural materials as well as environment-friendly pesticides. The inhibition effect on mycelia growth of gray mold is the highest when the gray mold mycelia were treated with a pesticide (commercial name: Koreayeok, Jihabudea KM, Sootingtan, Sootingstar) that contains a mixture of Bacillus subtilis, resulting in 100% inhibition of the mycelia growth. Meanwhile, the range of less than 20% inhibition effects on the growth of gray mold mycelia was observed with other commercial agricultural materials. The significant inhibition effects on spore germination of gray mold fungus were shown in vitro with two water dispersible pesticides containing sulfur [BTB (97.7%) and SulfurStar (92.3%)], respectively. These in vitro results of inhibiting of the spore germination and mycelia growth together cannot found. It remains to be determined whether the selected environment-friendly agricultural materials in effective control of gray mold in vitro can be used to control gray mold in field.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.46-58
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• 2017

This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation and seedling growth in Microlepia strigosa (Thunb.) C. Presl. Spore germination and prothallus development were promoted by low concentrations of Knop medium nutrient solution. The optimal medium for prothallus propagation and antheridium formation was 2X MS medium with 3% sucrose. The activated charcoal content of the medium did not affect prothallus proliferation. Among the various combinations of culture soil (bedding soil, peat moss, perlite and decomposed granite), a mixture of bedding soil, peat moss and decomposed granite at a ratio of 1 : 1 : 1 (v : v : v) had a positive effect on sporophyte formation. The most efficient conditions for promoting the growth of whole plants (sporophyte seedlings) were 50 - cell plug trays filled with a mixture of bedding soil and decomposed granite at a 2 : 1 (v : v) ratio.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.210-214
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• 1997

The experiment was conducted to obtain basic informations on the effects of key environments and fungicides on the sporulation, conidial germination and hitial growth of the hyphae of grape powdery mildew fungus Uncinula necator. Maximum sporulation occurred at RH 75~96% in vitro adiusted with sulfuric acid solution. Conidial germination and initial growth of the hyphae were better at 5% water agar than distilled water, and best at $26^{\circ}C$ and pH 5.0 of the substrates. Germination occurred equally well at light intensity of below 100 lux or dark condition, which was better at water agar supplemented with leaf extract 30% than pure water agar. The water agar supplemented with triazole fungicides reduced conidial germinations and initial growth of the hyphae signifcantly, in proportion to the increase in concentration of the fungicides, but the maguitude of reduction depended on the fungicides. Particularly in myclobutanil, reduction rate was very low as increased in concentration.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.11a
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• pp.161-162
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• 2004

주암호와 상사호의 담수와편모조 P. bipes 휴면포자의 환경조건에 따른 발아특성을 밝하기 위하여, 매월 채집된 휴면포자를 다양한 수온, 광조건, 영양염, pH 등에서 발아실험을 실시하였다. 실험결과, P. bipes 휴면포자는 수온 $20^{\circ}C$, 광도 $20{\mu}E/m^2/s$, 풍부한 영양원, pH 6-8 등이 최적발아조건으로 밝혀졌다. 특히, 환경요인 중 수온과 광조건은 휴면포자의 발아에 상대적으로 좀더 큰 영향을 주는 것으로 판단된다.

• The Korean Journal of Mycology
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• v.21 no.3
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• pp.224-229
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• 1993

The optimum temperature for germination of conidia and germ tube elongation were between $20\;and\;30^{\circ}C$ in C. dematium and C. gloeosporioides. Appressoria were fairly formed well at $20^{\circ}C$ despite the delay of conidial germination. At $30^{\circ}C$, both the germination and germ tube elongation are favored, but appressoria were poorly detected to be formed. In C. acutatum, the optimum temperature for germination of conidia was from $20\;to\;30^{\circ}C$, but at $25^{\circ}C$, germ tube elongation are accelerated. The conidia become septate and one or both doughter cells become conidiogenous instead of producing germ tubes and a secondary conidia produced, resulting in an arborescent type of connected conidia. Appressoria are infrequently formed by germinating conida. At $20\;to\;25^{\circ}C$ was the optimum for appressorium formation. But conidia that germinated at $30^{\circ}C$ seemed to lose the ability to form appressoria. The relation of temperature to germination of conidia and appressorium formation in Colletotrichum acutatum, C. dematium and C. gloeosporioides are discussed.

• The Korean Journal of Mycology
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• v.41 no.4
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• pp.295-300
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• 2013

This study was conducted to test effect of fungicides on the spore germination and mycelial growth of BotaniGard$^{(R)}$(Beauveria bassiana GHA). Eight fungicides, which are using for plant disease control in cucumber cultivation, with different concentration were mixed with conidia of GHA isolate for 0.5, 2, and 4 hours. The fungicides trifluminazol, fenarimol, ethaboxam, copper hydroxide and tetraconazol did significantly not inhibit on spore germination and mycelial growth. The trifluminazol, propineb and tetraconazol on high concentrations inhibited the mycelial growth of GHA. However, mixing time of fungicide with conidia of GHA did not affect in spore germination and mycelial growth. These results suggest that fungicide effect needed be checked before mycopesticide is applied in the field.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.41-46
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• 1986

Exogenous factor and nutrients affecting for conidial germination of Colletetrichum dematium f. sp. capsicum causing red pepper anthracnose were studied by slide germination test. Optimum temperature of conidial germination was at $28^{\circ}C$, ranging 15 to $35^{\circ}C$. Optimum pH was at 5.5, ranging 4.5 to 8.0, and more than 90% of relative humidity (RH) was optimum. Poor conidial germination of the fungus was observed on sterile distilled water, but potato sucrose broth (PSB), red pepper fruit broth (RPFB), green pepper fruit broth (GPFB) and pepper leaf broth (PLB) furnished a satisfactory nutrients for conidial germination. Exogenous supply of carbon and nitrogen sources were essential for conidial germination, while potassium, phosphorous and sulfur were not evident as that for carbon and nitrogen. Soluble starch was the most suitable as a carbon source for conidial germination and followed by D-glucose, D-galactose and lactose in that order. Maximum germination was attained in the $1{\times}10^4$ conidia per ml. Germination was decreased with increment of conidial concentration, and in the density of $5{\times}10^4$ conida per ml, germination was nearly supipressed. It suggested existing a self-inhibitor. Non-washed conidia germinated more than washed conidia, and conidial germination was also gradually decreased by increasing conidial density.