• Journal of Bio-Environment Control
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• v.15 no.2
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• pp.190-195
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• 2006

The purpose of this study was to figure out the best condition in growth and sod quality of Mentha spp. according to cutting methods and soil depth. Applemint (M. suaveolens), Peppermint (M. piperita), and Spearmint (M. spicata) were used. Regardless of cultivar and soil depth condition, the growth rate in top cutting was higher than layering method. The difference between layering and layering without apical meristem was not significant. Optimal condition for growth was 5cm depth of soil. However, the shallower the depth of the soil, the better quality of sod. Among three Mentha species, M suavelens showed plant height and node number and M. piperita had shoot number were higher than other variety. The best condition of sod was top cutting and 1cm depth of soil regardless of cultivar.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.104-104
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• 2020

꼭두서니과 커피속 식물의주요 2차대사산물인 카페인은 커피나무, 카카오, 차나무 등에서 관찰되는 알칼로이드 화합물로 다른 식물의 발아 억제, 살충 기능이 알려져 있다. 인체에 있어 카페인은 각성효과가 있어 수면 장애나 불안을 유발할 수 있으며 섭취를 중단 시 졸음, 두통 등 금단 증상을 나타나기도 한다. 최근 다양한 식물 종과 부위를 이용한 차 문화가 확산되고 있는 바, 본 연구는 시중에서 판매되고 있는 커피 2종류과 녹차 2종류를 비롯하여 꽃차 37종류, 잎차 15종류, 씨앗차 2종류, 열매차 1종류 및 뿌리차 2종류를 대상으로 메탄올 추출 후 gas chromatography-mass spectrometry를 이용하여 카페인 함량에 대한 정성, 정량 분석을 수행하였다. 대조로 사용된 시판 커피 2개사 제품은 각각 1.18 mg/g, 35.94 mg/g의 caffeine 함량을 나타냈으며 시판 녹차 티백 제품 2종은 1.3 mg/g, 3.42 mg/g의 함량을 나타내었다. 조사된 37종의 꽃차 중에는 차나무 꽃차에서만 1.50 mg/g 수준의 caffeine이 검출되었을 뿐 국내 소비가 확대되고 있는 구절초, 국화, 금계국, 금목서, 금어초, 금잔화, 노랑코스모스, 당아욱, 도라지, 동백나무, 뚱딴지, 라벤다, 마리골드(노랑), 마리골드(주황), 매화, 맨드라미, 무궁화, 백목련, 벚나무, 복사나무, 비단향꽃무, 생강나무, 수레국화, 아까시나무, 연꽃, 유채, 작약, 장미, 진달래, 천일홍, 캐모마일, 패랭이꽃, 팬지, 해당화, 홍화, 히비스커스 등 36종에서는 카페인이 검출되지 않았다. 잎차의 경우 레몬그라스, 레몬밤, 로즈마리, 뽕나무, 세인트존스워트, 스테비아, 쑥, 애플민트, 연잎, 적양배추, 조릿대, 차수국, 티트리, 파인애플민트, 페퍼민트 등 평가된 15종류 모두에서 카페인이 검출되지 않았으며, 씨앗차인 펜넬 및 작두콩, 열매차인 진피차, 뿌리차인 자색당근과 비트차에서 역시 카페인이 검출되지 않았다. 이상의 결과에 기초할 때, 시판되는 다양한 식물 종과 부위를 이용한 차의 경우 대부분 caffeine을 함유하지 않음을 알 수 있었다.

• KSBB Journal
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• v.8 no.5
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• pp.495-503
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• 1993

To investigate the characteristics of growth and oil production of peppermint cells during a batch culture, cells derived from peppermint callus was cultivated in an air bubble reactor. During the batch culture, effects of inoculum size, abiotic stress, yeast elicitor, and two stage culture on the cell growth, the productivity of oleolesin, and the formation of flavor components were determined and also the sugar concentrations and kinetics of cell growth were analyzed. Among the various sizes of inoculum, the culture with 2.0% packed cell volume inoculum showed the optimum condition for cell growth in the proposed bioreactor, and the cell yield and essential oil production reached to 5.7g/1 and 0.109g/1, respectively. When the abiotic stress of daily 8hr dark and $10^{\circ}C$ cold treatments were given to the culture cell growth decreased but essential oil production increased to 0.546g/l. In a modified Lin-Staba medium in which 100mg/l yeast extract as an elicitor was added to the culture, the cell growth and oil production increased, and menthol content was 22.5% of oil. In the two stage culture, in which the basic culture conditions of 27$^{\circ}C$, light, and without elicitor were employed during the first six days followed by the second stage with daily 8hr treatment of cold and dark condition, and also with yeast extract as an elicitor, cell growth decreased after eight days, essential oil production was not increased, and menthol was not detected. Dry cell yield was 0.38g dry cell/g sugar and specific growth rate was 0.25 day-1. The major terpenoid in the oil was not the menthol but pulegone and piperitone, precursors of menthol were accumulated. However, when yeast elicitor was added, menthol was produced to the level of 22.5% which was the highest value in the peppermint cell culture reported so far.