• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.99-102
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• 2001

Recombinant Saccharomyces cerevisiae strains harboring various copy numbers of hirudin gene were developed to study dependency of hirudin expression level on its gene copy number. A linear relationship between the copy number of hirudin expression cassette and hirudin expression level was observed up to 10 copies. A double <5-integration vector truncated wi 디 1 the unnecessary bacterial genes before yeast transformation showed a four-fold increase in transformation efficiency and a 1.3-fold enhancement in hirudin expression level compared with a single <5 system. Gratuitous hirudin expression strain was developed by disrupting the GALl gene of S. cerevisiae. Glucose that was fed in a limited manner effectively supported cell growth and hi겨din expression by the gratuitous strain. Effects of methanol concentrations on hirudin production in recombinant Hansenula polymorpha were investigated in continuous and fed-batch cultures. At a steady-state of continuous culture, an optimum methanol concentration of 1.7 g/L was determined at a dilution rate of 0.18 $h^{-1}$ with 1.8 mg/L ${\cdot}$ h hirudin productivity.

• Spatial Information Research
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• v.11 no.3
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• pp.203-212
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• 2003

An integrated 3D GIS-based approach for understanding underground environment is proposed and applied to a land subsidence in densely populated region. Bedrock and geological discontinues were treated as main factors in this study. Because land subsidence in this study area was caused by cavity owing to dissolved limestone in percolating ground water. Ground was classified according to bedrock types using a clustering method and geological information, N value, and RQD value of boreholes were visualized and integrated by 3D-GIS. Therefore it was possible to recognize underground space easily and analyze the ground information effectively.

• Explosives and Blasting
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• v.23 no.2
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• pp.15-21
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• 2005

Already, VARI-STEM, CAS-BAG, POWER DECK etc. are used by various blasting methods to take the advantage of air decking technology in abroad. This study analyzed the application and the effect of Air ball blasting method in domestic blasting area. It is shown that blasting vibration was showed decrease about $30\~40\%$ and quantities of explosives was shown decrease about $20\~25\%$. Also, in the case of using air ball blasting method, it is found for airblast to be lower than common blasting method.

• The KSFM Journal of Fluid Machinery
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• v.17 no.4
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• pp.53-58
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• 2014

Vacuum pump transfers waste that is pulverized by integrated macerator. For this reason, unlike ordinary pump systems, there is a rotating macerator ahead of impeller for pulverizing. It is hard to predict numerical solution because area of Inlet flow path changes according to the rotation angle of the integrated macerator. So, in this study, the verification of performance evaluation method of Marine vacuum pump were numerically studied by commercial ANSYS CFX 13.0 software. We select a model of performance evaluation for study, and we analyze change of inlet flow path of integrated macerator according to rotation angle. We generate 5 model sets according to rotation angle of the integrated macerator. And we evaluate their performance by numerical analysis. Then, we analyze internal flow field and performance according to rotation angle of the integrated macerator based on numerical analysis result. In addition, we compared with experimental data for validity of numerical result by using steady state analysis.

• Proceedings of the KSEE Conference
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• 2008.10a
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• pp.17-28
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• 2008

국책사업이나 SOC의 확충을 위한 도로 및 철도의 건설에서 적용되는 터널의 단면크기를 보면, $50m^2$에서부터 $100m^2$이상의 중 대단면 터널이 주를 이루고 있으나, 전력구, 통신구, 소규모로 운영되는 광산의 채광용 터널, 용수를 위한 도수로터널 등 특수한 용도로 설계, 시공되고 있는 터널에서는 $20m^2$이하의 단면크기를 갖는 경우가 있다. 이러한 소단면 터널의 경우에는 협소한 작업공간으로 인하여 적용공법 뿐만 아니라 장비의 사용 또한 제약을 받게 되어 작업효율이 저하되고 공사기간이 늘어나게 되는 등 여러 가지 문제점을 안고 있다. 특히, 에멀젼 폭약을 사용하는 발파에서 먼저 기폭된 발파공의 충격압력에 의해 인접공의 폭약이 예비압축(Precompression)되어 사압현상을 일으키고 잔류약을 발생시키는 사례가 종종 발생하고 있다. 사압현상은 당해 발파의 실패와 함께 2차적인 사고의 위험요인이 될 수 있으므로 이를 방지하기 위한 대책을 수립하여야 한다. 이를 위해 기존 문헌을 통하여 사압현상의 원인과 발생 가능성을 검토하였고, 국내에서 주로 사용되는 에멀전폭약의 수중 내충격성시험과 충격압력 전달시험을 실시하여 사압현상의 발생정도를 측정하였으며, 사압현상이 발생한 소단면 터널현장을 대상으로 대책을 수립하여 적용하였다. 심발방법을 변경하여 전단의 충격압력을 견딜 수 있는 공간격을 확보하고 뇌관의 초시간격을 적절하게 배치한 발파패턴을 적용한결과, 사압현상을 억제하고 잔류약의 발생을 감소시켜 계획 굴진장을 확보하고 파쇄석의 크기를 감소시키는 등 양호한 결과를 얻을 수 있었다.

• Journal of the Korean Geotechnical Society
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• v.19 no.2
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• pp.217-225
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• 2003

The ground reinforcement effect of pressure grouting depends on grout penetration into ground. It is not, however, easy to predict the grout penetration in the design process because of the heterogeneity of ground conditions. This study investigates the proper grouting pressure and grouting method through laboratory model tests for pressure grouting using loose to medium dense crushed rock and sandy ground using specially designed and fabricated device. The optimum injection pressure, grout quantity and injection time are investigated through performing pressure grouting under changing conditions of injection in this test. From the test results, it was found that optimum injection pressure covers the range of 3 to 4kg/cm$^2$.

• Explosives and Blasting
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• v.39 no.3
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• pp.15-23
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• 2021

Ventilation shafts are pathways in mines and tunnels for the removal of dust or smoke during underground space construction and operation. In mines, blasting with long blast holes is preferred for the excavation of a ventilation shaft in the 10~20m long crown pillar section. In this case, the bottom part of the blast hole is completely drilled in order to determine the drilling error, and this causes a problem of lowering the explosive charge and blasting efficiency. It is possible to solve the problem of explosive loading and to increase the blast efficiency by covering the curb of the blasthole by using stemming material. In this study, simulations for the blasting of a ventilation shaft were performed with various stemming lengths and the blasthole diameters(45, 76mm) using AUTODYN 2D SPH(Smooth particle hydrodynamics) analysis technique. Also the optimal bottom stemming column was derived by checking the size of the boulder and burden line according to blasting. Analysis result, blasting efficiency is lessened in case of stemming length less than 30cm and the optimal length of the stemming material should be 30cm or higher to achieve high efficiency of blasting.

• Explosives and Blasting
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• v.29 no.2
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• pp.13-31
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• 2011

Air deck charge blast methods have been applied to improve the fragmentation in open cut bench blastings for mining developments. However, during large scale bench blasting operations, there exist some problems such as boulder productions due to explosive charge concentration. Especially, in case of lime stone mining, when air deck method is applied, there has been unintentionally concentrated on charging because the inside holes are often broken by erosion and decomposition. In this study, compared with general blasting, air deck blasting has been focused in lime stone mining. In other to maximize its efficiency, inside hole was examined by endoscope in advance and deck charge using air tube was applied to the section in which concentration might be taken place. Blasting efficiency, fragmentation, charging reduction rate, and total working hours (from charging to blasting) were the main object for comparing, and as a result, air deck was more efficient then the general blasting in all aspects except total working hours.

• Journal of Korean Tunnelling and Underground Space Association
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• v.17 no.5
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• pp.533-551
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• 2015

The rock fracturing during waterjet cutting is very complicated because rock is inhomogeneous and anisotropic, compared with artificial materials (e.g., metal or glass). Thus, it is very important to verify the effects of rock properties on waterjet rock cutting performance. Properties affecting the rock cutting efficiency have been variously described in the literature, depending on the experimental conditions (e.g., water pressure, abrasive feed rate, or standoff distance) and rock-types studied. In this study, a rock-property-related literature review was performed to determine the key properties important for waterjet rock cutting. Porosity, uniaxial compressive strength, and hardness of the rock were determined to be the key properties affecting waterjet rock cutting. The results of this analysis can provide the basic knowledge to determine the cutting efficiency of waterjet rock cutting technology for rock excavation-related construction.

• Journal of the Korean Applied Science and Technology
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• v.32 no.4
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• pp.616-622
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• 2015

Current ballast water treatment technologies are applying chemical or electrical treatment technology which are not free from secondary environmental pollution. The purpose of this study is to treat the ballast water by shear stress without an additional environmental pollution and to find out the optimal treatment apparatus. We tried to treat ballast water by applying shear stress with two different type of combination of inner and outer cylinder, such as non-pattern type and groove type. In the case of non-pattern type of inner and outer cylinder, sterilization effect was comparatively low because of a slip between inner and outer cylinder. But in the case of groove type of inner and outer cylinder, sterilization effect was superior to the non-pattern type. With a same revolutional speed of 8000rpm, an extinction effect was acquired in the gap of 1 mm of inner and outer cylinder at non-pattern type, but 3mm of that of groove type.