• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.249-257
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• 2017

Agastache rugosa, is a perennial medicinal plant commonly used in Chinese herbalism, and may have anti-atherogenic and antibacterial properties. Here in this study, we investigated the growth and variations in antioxidant contents of A. rugosa in response to nutrient solution and photosynthetic photon flux density (PPFD) with artificial lighting for a hydroponics culture. Fluorescent light at 150, and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with a 16/8 (light/dark) photoperiod, combined with four different nutrient solutions [developed by Horticulture experiment station in Japan (HES), University of Seoul (UOS), Europe vegetable research center (EVR), Otsuka-house 1A (OTS)], were used in a hydroponics culture system for 6 weeks. The shoot and root dry weights of A. rugosa grown with the OTS were significantly higher than those of other nutrient solutions. The amount of tilianin was the highest grown with the OTS, followed by EVR, HES, and UOS. Total acacetin content was the highest in A. rugosa grown under EVR which was statistically similar with OTS. The A. rugosa grown under $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD produced higher fresh weight and both acacetin and tilianin contents than that grown under $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD. The present results suggested that OTS along with $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD could be an optimum growing condition for better growth and higher accumulation of tilianin and acacetin contents in A. rugosa with hydroponic culture systems in a plant factory.

• Journal of Bio-Environment Control
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• v.29 no.2
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• pp.202-208
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• 2020

The objective of this study was to select the right species among Agastache in the family Lamiaceae as the result of growth analysis and functional substance analysis. Among 22 species of Agastache, five species (Agastache cana, Agastache foeniculum, Agastache rugosa 'Spike Blue', Agastache rugosa 'Spike Snow', Agastache rupestris) were selected by referring to United States Department of Agriculture (USDA)'s plant hardiness zone area and Korea's one based on USDAs. These plants were cultured at 24 ± 1℃ and 18 ± 1℃ (day and night temperatures, respectively) and 65 ~ 75% relative humidity in a hydroponic culture system for 4 weeks. In terms of growth analysis experiment, stem length, root length, leaf width, leaf area, leaf number, SPAD value, and fresh and dry weights of shoots and roots were measured. The results showed that A. rugosa SS and A. rugosa SB have a higher overall balance value than other species. When compared functional substance value (rosmarinic acid, tilianin, and acacetin) of five species, A. rugosa SS and A. rugosa SB are significantly higher than other species in Agastache. It seems to be directly proportional to the growth analysis results. When considering the growth and functional substance part comprehensively, A. rugosa SS and A. rugosa SB are the most optimal high-value species among Agastache.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.319-324
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• 2022

The objective of this study was to determine the proper night interruption of photoperiods and dark periods for accumulating bioactive compounds of Agastache rugosa without decreasing plant growth. Five-week-old seedlings were transplanted in a DFT system with white LEDs. A. rugosa was treated with night interruption time treatments of 18:1:2:3, 18:2:2:2, 18:3:2:1 (light:dark:light:dark), and 20:4 (control) for 4 weeks. There were no significant differences except for leaf length, leaf width, and the number of flowers. The content of antioxidants in the shoot of A. rugosa was high in tilianin and acacetin, and the content of rosmarinic acid (RA) was significantly higher in the underground part. The RA content per dry weight of A. rugosa was 47.92 and 51.46% higher than that of the control in 18:1:2:3 and 18:2:2:2, and tilianin and acactin per dry weight were significantly higher in 18:3:2:1. There was no significant difference in growth due to the same day light integral, but 18:2:2:2 showed high total polyphenol contents. Therefore, it is thought that the effect of increasing secondary metabolites of A. rugosa without degradation of growth can be expected through night interruption treatment in plant factory cultivation systems using artificial light.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.260-268
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• 2018

The aim of this study was to evaluate the effect of light quality using either monochromatic or combined LEDs on the growth and antioxidant accumulation of Agastache rugosa cultivated under hydroponics for 4 weeks. This experiment was performed in a controlled-environment room at $22{\pm}1^{\circ}C$ and $18{\pm}1^{\circ}C$ (day and night temperatures, respectively) and 50-70% relative humidity, with a provided photosynthetic photon flux density (PPFD) of $180{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and irradiated with either monochromatic (W10 and R10) or mixed LEDs (W2B1G1, R3B1, R2B1G1, and W2B1G1) with a differing ratio of each LED's PPFD and fluorescent lighting (FL: control) with a 16/8 h photoperiod. Fresh and dry weights were highest for plants grown under the W2B1G1 treatment. A. rugosa grown with R10 had the greatest plant height but the lowest SPAD among all treatments. The concentration of rosmarinic acid in plants grown under W2B1G1 was significantly higher than that of plants grown under other treatments. Tilianin content was significantly higher in R3B1 than in the other treatments. However, whole-plant rosmarinic acid and tilianin content was the highest under the W2B1G1 condition. To cultivate A. rugosa in a plant factory, mixed-LED light conditions with W2B1G1 is considered to be more advantageous for the growth and antioxidant accumulation of A. rugosa. It is though that the total whole-plant antioxidant content is more crucial for commercial use; the present study demonstrates the potential to achieve higher content of functional materials in plants through the selection of light quality.