• Korean Journal of Ichthyology
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• v.34 no.3
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• pp.208-217
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• 2022

We wanted to develop a real-time PCR assay capable of detecting Liobagrus obesus in environmental DNA (eDNA) extracted from freshwater samples using a pair of species-specific primers and probe for the endangered fish, L. obesus. The species-specific primers and probe were designed in consideration of single nucleotide polymorphisms between 65 species of freshwater fish living in the Republic of Korea within the cytochrome b (cytb) gene of mitochondrial DNA. The species-specific primers and probe, in the real-time PCR assay, showed high specificity as only the L. obesus genomic DNA (gDNA) was found to be positive in the specificity verification using 65 species gDNA of freshwater fish in the Republic of Korea. In addition, in the detection limit analysis using the serial dilution concentrations of L. obesus gDNA, it was found that it was possible to detect up to 0.2 pg, showing high sensitivity. Afterwards, using the species-specific primers and probe, real-time PCR assay was performed on freshwater samples obtained from 8 stations in the mid-upper basin of Geum River. As a result, the cytb gene of L. obesus was detected in total 5 stations including all 3 stations where this species was collected at the time of field survey. Therefore, the species-specific primers and probe developed in present study, and the real-time PCR assay using them, can accurately detect the cytb gene of L. obesus from eDNA samples, which can be utilized to monitor the existing habitats of this species and to discover potential new habitats.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.745-749
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• 2012

국내 주요하천은 지속적인 산업화와 난개발로 인해 생태계파괴 및 수변생물의 서식처 환경 악화로 하천 생태계에 많은 악영향을 받고 있다. 그러나 1990년대 이후 국내에서는 하천의 기능과 생물의 다양성, 하천환경 및 생태학적인 가치와 중요성에 대한 인식이 매우 커짐에 따라 하천의 생태적 기능을 매우 중요시 하고 있으며, 하천의 난개발로 인한 피해를 복원하기 위해 활발한 연구가 진행되고 있다. 대표적인 연구로 생태계 건전화 개념을 도입하여 담수성 어류 서식처 제공 및 이동에 필요한 유량을 산정한 한국 수자원공사(1995)의 연구가 있으며, 김규호 등(1996a, 1996b)은 물고기 서식처 환경 중 특히 수심과 유속 등 수리조건을 유지하기 위한 최소유량 산정 방법을 제시하였다. 또한 노경범(2011)은 섬진강의 하천환경 회복을 위한 연구대상 구간에서의 대표어종을 선정하여 서식처 특성 파악을 위해 하천 특성을 연구하였다. 이와 같이 현재 국내에서도 하천의 기능과 생태학적 기능을 위한 노력과 연구가 진행되고 있다. 그러나 생태학적 서식처 연구에서는 아직까진 미진한 부분이 많이 있다. 그 예로 보호종이나 멸종위기어종과 같은 생물의 서식처 확보를 위한 연구가 아직까지는 많은 노력과 연구가 필요한 상태이다. 본 연구에서는 환경부에서 지정한 멸종위기 1급 어종인 퉁사리를 대상으로 서식환경 특성 연구를 진행하였으며, 퉁사리의 생태학적 서식특성과 현재 지석천 상류 유역에서 관찰되고 있는 서식구간을 중심으로 MD-SWMS (Multi-Dimensional, Surface Water Modeling System) 모형을 이용하여 수리특성 분석을 수행하였다. 이를 위해 하상재료 입도분석을 실시하였으며, 그 결과 입도가 큰 굵은모래 및 잔자갈, 굵은자갈로 구성되어 있음을 알 수 있었다. 또한, 수리학적 특성분석결과 서식구간 내에서는 비교적 빠른 유속이 나타났다. 이러한 퉁사리의 서식환경의 특성 분석으로 서식구간 내 입도가 큰 하상재료로 구성되어 있으며, 퇴적구간이 생성되지 않을 정도의 유사이송량과 유속이 필요할 것으로 분석되었다. 향후 퉁사리의 장기적인 서식환경 조성과 보존을 위해 대상구간의 유사이송의 연구와 실측 및 수변환경 모니터링을 통한 다양한 연구가 진행되어야 할 것으로 판단된다.

• Korean Journal of Ichthyology
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• v.18 no.3
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• pp.209-215
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• 2006

A closely associated set of characteristics was analyzed using both classical and truss dimensions to discriminate the morphometric traits of three species of torrent catfish (Amblycipitidae) in Korea. After arcsine square root transformation, 18 measurements relative to the standard length and three measurements relative to the head length of each species of torrent catfish were compared statistically. Classical and truss dimensions of each of four morphometric traits showed higher values in the bullhead torrent catfish, Liobagrus obesus, than the Korean and south torrent catfish (L. andersoni and L. mediadiposalisa, respectively; P<0.05). Truss dimensions of three morphometric traits were higher in the bullhead and Korean torrent catfish than in the south torrent catfish (P<0.05). There were no significant differences in six morphometric traits among these Amblycipitidae. The dimensions used in this study may be useful as taxonomic indicators to discriminate species of Liobagrus in Korea.

• Korean Journal of Ichthyology
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• v.29 no.2
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• pp.89-93
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• 2017

For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84 FN (fundamental number), with having no polyploidy and sexual dimorphism. The chromosome number was the same as other closely-related species, L. mediadiposalis and L. somjinensis, but their karyotypes showed a clear difference by species. Such result may be related to chromosomal rearrangements by Robertsonian rearrangement with geographical isolation.

• Korean Journal of Ichthyology
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• v.19 no.2
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• pp.112-119
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• 2007

A histological study on development and transformation of the oocyte' follicle cell for Korean four sillurid fishes, Liobagrus obesus, L. mediadiposalis, Pseudobagrus koreanus, and P. brevicorpus was performed by light and electron microscopes. The follicular layer surrounding the oocyte consisted of an outer theca cell and an inner follicle cell (granulosa cell). The follicle cells of the oocyte were flatten cells at early oocyte but during vitellogenesis they were transformed it to a single layer of cuboidal cell, then to a single columnar cell layer, and finally to a layer covered with a substance secreted by themselves. Although the development and transformation of the follicle cells was similar to four species, the secreted materials, called an adhesive membrane, were divided into two types in its appearance and nature. Firstly, a jelly coat-like type was found in L. obesus and L. mediadiposalis, which they are presumed to be polysaccharides and mucoproteins in its nature and secondly, a granular type in P. koreanus and P. brevicopus, being mucoprotein. A zona radiata with about $0.6{\sim}3.1{\mu}m$ thin was present below the adhesive material secreted by the transformed-follicle cell's activity. The zona radiata was composed of two layers, a thin externa and a thick interna.

• Korean Journal of Ichthyology
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• v.24 no.3
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• pp.183-190
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• 2012

The spawning site characters in the natural environment of bull-head torrent catfish, Liobagrus obesus, were investigated at the part of the Gosan stream in Korea from April to October 2010 and June 2011. The sex ratio of female to male was 1 : 1.02. Spawning season was from June to July with water temperature in approximately $23^{\circ}C$. The spawning sites were covered by the boulder used upper plate and were composed of gravel and sand. One male lies with the egg mass and guards the developing embryos in the hollow below the boulder. The environmental conditions of the spawning sites were $61.4{\pm}11.97$ (50~85) cm in depth, 0.58${\pm}0.067$ (0.48~0.72) m/sec in surface water velocity, $0.46{\pm}0.098$ (0.27~0.61) m/sec in middle water velocity, $0.27{\pm}0.083$ (0.14~0.41) m/sec in bottom water velocity. The boulder width as spawning sites was $26.2{\pm}5.32$ (20~38) cm in long axis, $20.5{\pm}2.97$ (16~25) cm in short axis and $11.1{\pm}4.02$ (5~19) cm in height. The hollow underneath the boulder was $9.8{\pm}2.32$ (6~14) cm in diameter and $2.8{\pm}1.10$ (1.5~5) cm in depth. The average number of eggs in ovary was $124{\pm}27.7$ (92~180). The matured egg size was $3.40{\pm}0.078$ (3.21~3.56) mm. The average number of spawning eggs in the spawning site was $99{\pm}12.9$ (81~122).

• Development and Reproduction
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• v.10 no.1
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• pp.41-45
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• 2006

The spawning behavior of Liobagrus obesus was observed at Kumgang river, Yeongdong-gun, Chungcheongbuk-do from Apirl to July 2004. The fertilized eggs collected by dip net and skimming net were carried to the laboratory of Chonnam National University, and then egg, larvae and juvenils development were studied. Hatching of the embryo began about at 225 hrs 15 mins after morula stage in water temperature of $19.5{\sim}24.9^{\circ}C$(mean $22.8^{\circ}C$). The newly-hatched larvae were $7.30{\sim}7.90mm$(mean 7.66mm) in total length (TL), their mouth and anus were already opened with 14+28=42 myotomes. Sixteen days after hatching, the postlarvae were $13.00{\sim}14.05mm$(mean 13.48mm) TL, the yolk sac was completely absorbed. The juvenile stage was reached when all fin-rays were formed at 24 days after hatching, and $15.31{\sim}17.20mm$(mean 16.31mm) TL.

• Korean Journal of Environmental Biology
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• v.17 no.1
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• pp.79-88
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• 1999

The fish fauna, notified species, and fish community was studied at the six stations in a 18km section of the Ungchon stream, a mountain stream in Korea, where a new dam is planned from 1994 to 1996. Twentynine species (11 families, 25 genera) were found. Nine species were endemic to Korea. The Dominant specie in the studied area were Zacco temmincki, Z. platypus and Squalidus gracilis majimae. Some of the korean endemic species (Coreoperca herzi) including the Korean endangered species such as Pseudopungtudgia nigra and Liobagrus obesus were not found in this stream anymore. The average of area density was 0.69 individuals per square meter and the volume density was 1.45 individuals per cubic meter. Species diversity was highest in the middle region. According to community correlation coefficients of six stations, similality coefficients of station one, four, five and six were higher than others, but no significant difference in the composition of fish fauna was found between six study staions.

• Korean Journal of Environment and Ecology
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• v.19 no.1
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• pp.46-54
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• 2005

The change of fish community in the Wonju-stream was investigated from May to November, 2004. During the period,24 species belonging to 6 families were collected. There were 10 Korean endemic species$(41.7\%)$, including Pseudopungtungia tenuicorpa, Coreoleuciscus spiendidus, Squalidus gracitis majimae, Squalidus japonicus coreanus, Microphysogobio yaluensis, Microphysogobio longidorsalis, Iksookimia koreensis, Koreocobitis rotundicaudata, Liobagus andersoni and Odontobutis interrupts. The dominant species was Zacco Platypus$(54.7\%)$, and the subdominant species was Rhynchocypris oxycephalus$(16.7\%)$. Also, Carassius auratus$(5.4\%)$, I. koreensis$(3.4\%)$, Orthrias toni$(3.3\%)$, and Pungtungia herzi$(3.0\%)$ appeared. According to the fish species compositions, the fish community was divided into 2 groups by an unweighted pair group method using arithmetic averages(UPGMA). The comparison of the fish community showed that benthic fish species were decreased, while, the number of water-column species, resistant 1,o substrate changes and water pollution were increased.

• Korean Journal of Ecology and Environment
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• v.54 no.2
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• pp.130-141
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• 2021

By reviewing previous literature, we analyzed the food sources of 20 out of 29 endangered fish species from freshwater ecosystems in South Korea. A total of 19 studies reported that food sources of 20 endangered fish species included 20 phyla, 31 classes, 58 orders, 116 families, and 154 genera. Arthropod, insecta, diptera, and chironomidae were the most fed animal food sources according to different resolution of taxa index on phylum, class, order and family. Similarity, bacillariophyta, bacillariophyceae, naviculales, and cymbellaceae were the most fed abundant plant sources. A larger number of fish species were reliant on animal food sources than plant food sources. 18 of the endangered fish preyed on arthropods, whereas only 6 species consumed bacillariophyta. To characterize the feeding groups of the 20 fish species, a hierarchical clustering analysis and non-metric multidimensional scaling analysis were conducted. The fish species were divided into two groups: 1) insectivores and 2) planktivores. A network analysis, which associated the link between endangered fishes and food sources, also revealed the same two groups. The highest hub score of food sources was for macroinvertebrates, including diptera (0.47), ephemeroptera (0.42), and trichoptera (0.38), based on the network analysis. Niche breadth was used to calculate the diversity of the food sources. Phoxinus phoxinus (0.57) showed thehighest food source diversity among the fish species, whereas Iksookimia pacifica (0.01) showed the lowest. This study will be utilized for the conservation and restoration of the endangered fish species.