• Microbiology and Biotechnology Letters
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• v.17 no.3
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• pp.207-212
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• 1989

Cultural conditions for $\alpha$-D-Glucosidase Inhibitor production from Streptomyces sp. YS-221-B isolated from soil arid identified as Streptomyces flauovirens or a subspecies of it were investigated. When the strain was cultured in a flask containing 2% glucose, 0.3% asparagine, 0.0002% riboflavin, 0.05% $K_2$HPO$_4$, 0.1% MgSO$_4$.7$H_2O$, 0.05% NaCl, pH 8.0 at 3$0^{\circ}C$, maximum production of the inhibitor was obtained after 8-9 days of cultivation. Sugar alcohols such as mannitol, i-inositol, erythritol as carbon sources, asparagine and beef extract as nitrogen sources were favorable for inhibitor production. Among vitamins, riboflavin, p-aminobenzoic acid, pyridoxamine and folic acid promoted the production of inhibitor, but depressed by the addition of hesperidine, and also depressed by cobalt, lithium and ferrous salts.

• Korean Journal of Environmental Agriculture
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• v.38 no.1
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• pp.17-22
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• 2019

BACKGROUND: Soil contamination of As is a very sensitive environmental issue due to its adverse impact on human health and different characteristics with other heavy metals. With public awareness of As poisoning, there has been growing interest in developing guideline and remediation technologies for As-contaminated soil. The objective of this research was to evaluate the effectiveness of stabilizing amendments and soil dressing methods on the mobility of As in the contaminated rice paddy soils nearby mining area. METHODS AND RESULTS: Different amendments were mixed with surface and subsurface contaminated soils at a ratio of 3% (w/w) and monitored for five months. Three different extractants including 0.01M $CaCl_2$, TCLP, and PBET were used to examine As bioavailability in the soil and the concentration of As in rice grain was also measured with an inductively coupled plasma (ICP) spectroscopy. The results showed that all amendment treatments decreased As concentration compared to the control. Especially, coal mine drainage sludge (CMDS) treatment showed the highest efficiency of decreasing As concentration in the soil and rice grain. The values of Pearson correlation (r) between As concentrations in the soil and rice grain were 0.782, 0.753, and 0.678 for $CaCl_2$, TCLP, and PBET methods, respectively. Especially, $CaCl_2$ method was highly correlated between As concentrations of the soil and soil solution (r=0.719), followed by TCLP (r=0.706), PBET (r=0.561) methods. CONCLUSION: Stabilizing amendments can effectively reduce available As concentration in the soils as well as soil solution, and thereby potentially mitigating risks of crop contamination by As.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.1
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• pp.127-134
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• 2018

This study evaluated the effects of high air temperature and waterlogging duration on growth and yield of Kimchi cabbage. Air temperature treatments were applied with ventilation; optimal (set $20^{\circ}C$) and delayed ($30^{\circ}C$) in the greenhouses. The waterlogging treatment levels were implicated 0, 12, 24, 48, and 72 hours, respectively. The growth of Kimchi cabbage was significantly affected by waterlogging duration. The head weight decreased by combining severe waterlogging and high air temperature. Net photosynthetic rate under the combination of non-waterlogging and optimal air temperature was $22.6{\mu}mol\;CO_2{\cdot}m^{-2}{\cdot}s^{-1}$, which was the greatest, while that of 72 hours-waterlogging was rapidly decreased. The percentage of formality with 0, 12, and 24 hours-waterlogging was over 88%, however, those of 72 hours-waterlogging with optimal and delayed ventilation were 64 and 68%, respectively, which were dramatically reduced. The yields were more affected by waterlogging duration than air temperature treatment because of deducting as increased waterlogging periods. These results indicate that waterlogging treatment reduced the yield and quality of Kimchi cabbage, thus it will be feasible to enhance the harvest time when severe waterlogging in the field.

• Journal of Environmental Science International
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• v.27 no.4
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• pp.251-259
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• 2018

This study was conducted to determine the effects of combination of air temperature and soil water content on the growth, physiological disorder rate, and yield of hot peppers. The study was carried out in a typical plastic house (open on one side and with ventilation fans on the other side), which was maintained with gradient air temperature (maximum difference in air temperature: $6^{\circ}C$). The deficit irrigation (DI) treatment commenced 65 days after transplanting. The height of plant and fresh and dry weights of the stem increased at high air temperature (ambient + $6^{\circ}C$, extreme high temperature; EHT). Furthermore, the leaf area decreased significantly with the DI treatment. There were no significant differences in the stem diameter, number of branches, and fresh and dry weights of the leaves among all the treatments. The net photosynthesis rate of the full irrigation (FI) treatment was higher than that of the DI treatment. The photosynthesis rate at ambient air temperature was $19.7{\mu}mol\;CO_2m^{-2}{\cdot}s^{-1}$, the highest among all the treatments; however, the photosynthesis rate of the EHT treatment decreased by 60% ($12.3{\mu}mol\;CO_2m^{-2}{\cdot}s^{-1}$). Additionally, the formation of guard cells in the leaf was abnormal with the EHT treatment, and there was a decrease in translocation efficiency. The effects of air temperature treatment were more pronounced on the physiological disorder rate and yield. The physiological disorder rate of the EHT treatment was the highest under the DI treatment condition. The yield of the AFI (ambient air temperature with full irrigation) treatment was 3,771 kg/10a, the highest among all the treatments; however, the yield of the EHT treatment with DI and FI was 1,282 and 1,327 kg/10a, respectively. These results indicate that growth and physiological disorder rate improved with the EHT treatment; however, there was a decrease in yield. Furthermore, the formation of guard cells was abnormal and malfunctional.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.260-267
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• 2016

Amino acids in the plant were intermediate metabolites which produced by uptake and assimilation of nitrogen and these extracts which gained by bio-chemical digestion from protein of plant or animal were a source of functional fertilizer. This study was conducted to evaluate effects of keratin amino acid fertilizer (KAF) gained from animal hair or hoof on changes of turfgrass quality and growth by investigating turf color index, chlorophyll index, shoot number, clipping yield, and nutrient content in the turfgrass tissue. Treatments were designed as follows; non-fertilizer (NF), compound fertilizer (CF), keratin amino acid fertilizer treatments [CF + KAF $0.26ml\;m^{-2}$ (CKF), CF + KAF $0.52ml\;m^{-2}$ (2CKF)], and only keratin amino acid fertilizer treatment (KF). Shoot number, clipping yield, and nitrogen uptake of KF were higher than those of CF. As compared with CF, soil chemical properties, turf color index, chlorophyll index and clipping yield of keratin amino acid fertilizer were not significant, but shoot number and uptake of N and K were increased significantly. These results show that the application of keratin amino acid fertilizer increased shoot number and growth by increased uptake of nitrogen and potassium.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.242-248
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• 2007

To find a feasibility of utilization of food waste slurry (FWS) generated during composting, FWS was combined with pig manure slurry (PMS) in various ratios and the change of nutrient contents and offensive odor of the combined slurries before and after fermentation were studied. The initial pH was 7.67 for PMS and 8.45 for FWS. However, during the fermentation, pH increased in the combined slurries with the higher FWS rate among the treatments while decreased in thosewith higher PMS rate. EC of each slurry sample showed that the difference among combined slurry samples has been reduced during fermentation and became stabilized in $21{\sim}23dS\;m^{-1}$ after 180 days. After 180 days fermentation, total nitrogen (T-N) decreased. T-N of mixture with a half and more FWS decreased up to 0.1%, less than the critical level (0.3%). The contents of O.M., T-N, phosphorus, calcium and magnesium decreased with fermentation while those of potash and salinity increased. From initial fermentation until 30 days, a lot of $NH_3$, as an offensive odor, was produced. However, it decreased steadily, except in higher PMS rate. In terms of producing $50{\mu}g\;ml^{-1}$ of $NH_3$, the top layer took 30 days after fertilization with FWS only, 45 days for utilized treatment with F75 (25 % of PMS), 75 days for utilized with F50 (50%) and F25 (75%) and 90 days for PMS only, respectively. $RNH_2$ also had similar trend with $NH_3$ but it was produced continuously as long fermentation proceeded. In terms of $RNH_2$, the decrease in concentration up to $50{\mu}g\;ml^{-1}$ were; 45 days for FWS only(F100), 105 days for F75 utilization, 120 daysfor F50, 165 days for F25, respectively. ethyl mercaptan was produced in PMS until 180 days after fertilization but it was not produced in FWS. Sensory tests as an integrated test of offensive odor were also done. FWS showed lower than 1 after 30 days from initial fermentation, while PMS had still offensive odor even up to 180 days from initial fermentation. It is probably affected by the continuous production of ethyl mercaptan and amines. However, considering in decrease T-N content caused by volatilization while offensive odor intensity according to official standard of fertilizer is lower than 2. Further study on controlling offensive odor needs to be done.

• Economic and Environmental Geology
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• v.36 no.6
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• pp.441-455
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• 2003

The most common water types are found to be Ca-$HCO_3$, Ca-Na-$HCO_3$ and Ca-Na-$HCO_3$-Cl in Gwangju groundwater. Groundwater near the Gwangju stream are characterized Ca-Cl water type, with over 50 mg/L of C1- and 400 ${\mu}$S/cm of EC. The systematic variation of $Cl^-$, $HCO_3^-$,- EC and ${\gamma}^{18}O$ values in groundwater with distance away from drainages is caused by streamwater infiltration. Stable isotope data indicate that ${\gamma}$D and ${\gamma}^{18}O$ values of groundwaters near drainages were enriched by evaporation effect, showing a equation of ${\gamma}$D=7. 1${\times}{\gamma}^{18}O$-1. ${\gamma}^{18}O$ values over -6${\textperthansand}$ are anomalous in the unconfined groundwater zones, which are influenced by the local surface water enriched in $^{18}O$ composition. Groundwater in highland shows remarkably light ${\gamma}^{18}O$ values below -8$\textperthousand$. The infiltration of streamwater is dominant in unconfined alluvium aquifer near drainages. ${\gamma}^{13}$CDIC values (-17.6∼-15.2$\textperthousand$) of groundwaters near drainages revealed that dissolved inorganic carbon (DIC) is predominantly originated from natural soil-derived $CO_2$. ${\gamma}^{15}N$ and ${\gamma}^{18}O$ values of nitrate are 0∼17.0${\textperthansand}$ and 6.6∼17.4${\textperthansand}$, respectively. Relationship between ${\gamma}^{15}N$ and ${\gamma}^{18}O$ shows a systematic isotopic fractionation caused by denitrification of 40∼60%, suggesting that the major source of groundwater nitrate originated from nitrate of soils, and mixing nitrate of soil and sewage or manure.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.160-160
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• 2017

자동화 기술을 통한 한국형 스마트팜의 발전이 비약적으로 이루어지고 있는 가운데 무인화를 위한 지능적인 스마트 시설환경 관찰 및 분석에 대한 요구가 점점 증가 하고 있다. 스마트 시설환경에서 취득 가능한 시계열 데이터는 온도, 습도, 조도, CO2, 토양 수분, 환기량 등 다양하다. 시스템의 경계가 명확함에도 해당 속성의 특성상 타임도메인과 공간도메인 상에서 정확한 추정 또는 예측이 난해하다. 시설 환경에 접목이 증가하고 있는 지능형 관리 기술 구현을 위해선 시계열 공간 데이터에 대한 신속하고 정확한 정량화 기술이 필수적이라 할 수 있다. 이러한 기술적인 요구사항을 해결하고자 시도되는 다양한 방법 중에서 공간 분해능 향상을 위한 다지점 계측 메트릭스를 실험적으로 구성하였다. $50m{\times}100m$의 단면적인 연동 딸기 온실을 대상으로 $3{\times}3{\times}3$의 3차원 환경 인자 계측 매트릭스를 설치하였다. 1 Hz의 주기로 4가지 환경인자(온도, 습도, 조도, CO2)를 계측하였으며, 계측 하는 시점과 동시에 병렬적으로 공간통계법을 이용하여 미지의 지점에 대한 환경 인자들을 실시간으로 추정하였다. 선행적으로 50 cm 공간 분해능에 대응하기 위하여 Kriging interpolation법을 횡단면에 대하여 분석한 후 다시 종단면에 대하여 분석하였다. 3 Ghz에 해당하는 연산 능력을 보유한 컴퓨터에서 1초 동안 획득한 데이터에 대한 분석을 마치는데 소요되는 시간이 15초 내외로 나타났다. 이는 해당 알고리즘의 매우 높은 시간 복잡도(Order of $O=O^3$)에 기인하는 것으로 다양한 시설 환경의 관리 방법론에 적절히 대응하기에 한계가 있다 할 수 있다. 실시간으로 시간 복잡도가 높은 연산을 수행하기 위한 기술적인 과제를 해결하고자, 근래에 관심이 증가하고 있는 NVIDIA 사에서 제공하는 CUDA 엔진과 Apple사의 제안을 시작으로 하여 공개 소프트웨어 개발 컨소시엄인 크로노스 그룹에서 제공하는 OpenCL 엔진을 비교 분석하였다. CUDA 엔진은 GPU(Graphics Processing Unit)에서 정보 분석 프로그램의 연산 집약적인 부분만을 담당하여 신속한 결과를 산출할 수 있는 라이브러리이며 해당 하드웨어를 구비하였을 때 사용이 가능하다. 반면, OpenCL은 CUDA 엔진이 특정 하드웨어에서 구동이 되는 한계를 극복하고자 하드웨어에 비의존적인 라이브러리를 제공하는 것이 다르며 클러스터링 기술과 연계를 통해 낮은 하드웨어 성능으로 인한 단점을 극복하고자 하였다. 본 연구에서는 CUDA 8.0(https://developer.nvidia.com/cuda-downloads)버전과 Pascal Titan X(NVIDIA, CA, USA)를 사용한 방법과 OpenCL 1.2(https://www.khronos.org/opencl/)버전과 Samsung Exynos5422 칩을 장착한 ODROID-XU4(Hardkernel, AnYang, Korea)를 사용한 방법을 비교 분석하였다. 50 cm의 공간 분해능에 대응하기 위한 4차원 행렬($100{\times}200{\times}5{\times}4$)에 대하여 정수 지수화를 위한 Quantization을 거쳐 CUDA 엔진과 OpenCL 엔진을 적용한 비교한 결과, CUDA 엔진은 1초 내외, OpenCL 엔진의 경우 5초 내외의 연산 속도를 보였다. CUDA 엔진의 경우 비용측면에서 약 10배, 전력 소모 측면에서 20배 이상 소요되었다. 따라서 우선적으로 OpenCL 엔진 기반 하드웨어 가속 기술 최적화 연구를 통해 스마트 시설환경 실시간 시뮬레이션 기술 도입을 위한 기술적 과제를 풀어갈 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.200-206
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• 1996

Experiment was conducted to get the information about physico-chemical properties of flue-cured tobacco on the degree of maturity cultivated in paddy-upland rotated field, and compared to upland ones. For the samples of this experiment, 3~4 leaves at each stalk position were harvested from the bottom of plants. Physico-chemical properties of cured leaves were determined from the samples collected at weekly intervals, and of obtained from 4 stalk positions. For the degree of maturity, harvested leaves were separated with visual characters into four classes such as immature, mature, ripe, and mellow. Regardless of stalk position, the order of shrinkage rate with length and width of leaves was mellow> immature> ripe> mature, and ripe leaves from paddy field showed higher shrinkage rate than those of upland. Nicotine and total nitrogen contents were decreased with the degree of maturity while reducing sugar content were showed a reverse tendency. Ripe leaves from paddy field had lower reducing sugar contents, comparing with upland tobacco. Filling capacity of cured leaves from paddy field was decreased with degree of maturity, but there was no difference between upland and paddy tobacco. Shatter index was increased in the oeder of immature > mellow > mature > ripe. Chemical components of cigarette smoke from paddy field tobacco were little higher in $CO_2$ total particulate matter and tar contents, while combustibility was little lower than that of upland tobacco. It was also evaluated that paddy field tobacco was unfavorable for the non-volatile organic and higher fatty acids contents comparing with upland tobacco.

• Journal of Life Science
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• v.20 no.2
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• pp.269-274
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• 2010

An antifungal antibiotic-producing strain, BCNU 2003, was isolated from forest soil in Korea. The morphological and physiological characters, and 16S rRNA sequences analysis of strain BCNU 2003 identified this strain as Bacillus genus. The Bacillus sp. BCNU 2003 showed strong antifungal activities against Aspergillus niger, Trichophyton mentagrophytes and Trichophyton rubrum with inhibition ranging from 62.05 to 63.49% by using dual culture technique. Bacillus sp. BCNU 2003 produced a maximum level of antifungal substances under aerobic incubation at 28oC and pH 6.5-7.2 for 6 days in LB broth. Ethyl acetate extract of the cultured broth showed strong antifungal activity and a broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration (MIC) values for its active extracts ranged between 0.0625 mg/ml and 1 mg/ml. In addition, Bacillus sp. BCNU 2003 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase.