• Journal of KIISE:Computing Practices and Letters
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• v.15 no.5
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• pp.345-349
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• 2009

Social bookmarking systems are a typical web 2.0 service based on folksonomy, providing the platform for storing and sharing bookmarking information. Spammers in social bookmarking systems denote the users who abuse the system for their own interests in an improper way. They can make the entire resources in social bookmarking systems useless by posting lots of wrong information. Hence, it is important to detect spammers as early as possible and protect social bookmarking systems from their attack. In this paper, we applied a diverse set of machine learning approaches, i.e., decision tables, decision trees (ID3), $na{\ddot{i}}ve$ Bayes classifiers, TAN (tree-augment $na{\ddot{i}}ve$ Bayes) classifiers, and artificial neural networks to this task. In our experiments, $na{\ddot{i}}ve$ Bayes classifiers performed significantly better than other methods with respect to the AUC (area under the ROC curve) score as veil as the model building time. Plausible explanations for this result are as follows. First, $na{\ddot{i}}ve$> Bayes classifiers art known to usually perform better than decision trees in terms of the AUC score. Second, the spammer detection problem in our experiments is likely to be linearly separable.

• Journal of the korean academy of Pediatric Dentistry
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• v.28 no.4
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• pp.566-574
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• 2001

For decades it has been a clinically accepted requirement, in case of salivary contamination, to re-etch conditioned enamel and dentin to proceed with the adhesive technique. Only a few reports have been so far dealing with the potential of one-bottle adhesive system to bond even when applied after salivary contamination and without re-etching. The purpose of this study was to evaluate the influence of the salivary contamination on the shear bond strength of dentin. The results were as follows: 1. In group II, in which saliva contamination and washing occurred before applying of Prime & Bond NT showed high shear bond strength as in control group. In Group III and IV, in which the cured adhesive was contaminated with saliva, showed significantly lower mean bond strength, 2. Relating long resin tags of $70\sim120{\mu}m$ were observed in samples of all groups under SEM. We could observed hybrid layer, resin tag and many lateral branches in every group. And there were no differences between groups.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.6
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• pp.542-549
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• 2021

The purpose of this study was to provide an economical and easy preparation method for recombinant human epidermal growth factor (rhEGF) without the need for an expensive enzyme to cleave the fusion part. However, the N-terminal fusion part is still useful for affinity chromatography. The hEGF is an important hormone in cell growth and proliferation in humans, and many studies on the expression and purification of this protein have been reported. In the present study, the hEGF gene was designed to be optimized with the E. coli codon usage preference and to contain Asn-Gly at the N-terminus of the protein. The gene was inserted into pRSET_A, an E. coli expression vector, and transformed into E. coli BL21 (DE3). The recombinant fusion protein was successfully co-expressed with pG-Tf2, a chaperone vector, in E. coli and purified by Ni-NTA column chromatography. The rhEGF was then released by hydroxylamine treatment and confirmed by SDS-PAGE. ELISA analysis showed that the activity of the free rhEGF was more than 92% similar to that of commercial EGF. The biological activity of the rhEGF was confirmed by a cell proliferation test with human skin fibroblasts.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.49 no.1
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• pp.31-46
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• 2012

The most critical technology to implement ubiquitous health care is Ubiquitous Sensor Network (USN) technology which makes use of various sensor technologies, processor integration technology, and wireless network technology-Radio Frequency Identification (RFID) and Wireless Sensor Network (WSN)-to easily gather and monitor actual physical environment information from a remote site. With the feature, the USN technology can make the information technology of the existing virtual space expanded to actual environments. However, although the RFID and the WSN have technical similarities and mutual effects, they have been recognized to be studied separately, and sufficient studies have not been conducted on the technical integration of the RFID and the WSN. Therefore, EPCglobal which realized the issue proposed the EPC Sensor Network to efficiently integrate and interoperate the RFID and WSN technologies based on the international standard EPCglobal network. The proposed EPC Sensor Network technology uses the Complex Event Processing method in the middleware to integrate data occurring through the RFID and the WSN in a single environment and to interoperate the events based on the EPCglobal network. However, as the EPC Sensor Network technology continuously performs its operation even in the case that the minimum conditions are not to be met to find complex events in the middleware, its operation cost rises. Moreover, since the technology is based on the EPCglobal network, it can neither perform its operation only for the sake of sensor data, nor connect or interoperate with each information system in which the most important information in the ubiquitous computing environment is saved. Therefore, to address the problems of the existing system, we proposed the design and implementation of USN integration management system. For this, we first proposed an integration system that manages RFID and WSN data based on Session Initiation Protocol (SIP). Secondly, we defined the minimum conditions of the complex events to detect unnecessary complex events in the middleware, and proposed an algorithm that can extract complex events only when the minimum conditions are to be met. To evaluate the performance of the proposed methods we implemented SIP-based integration management system.

• Nuclear Medicine and Molecular Imaging
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• v.43 no.5
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• pp.487-494
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• 2009

Purpose: Small size of recombinant scFv antibody has many advantages such as rapid blood clearances and improved targeting antibodies to tumor region. On the other hand owing to small size, number of amino group is insufficient in conjugation with chelator and fluorescence labeling. This study is to introduce poly lysine tag to the C-terminal end of scFv lym-1 sequence for fluorescence chelator conjugation. Materials and Methods: Poly lysine scFv lym-1 gene, cloned into pET-22b (+) vector, was expressed in E. coli BL21 (DE3) strain. Antibody purification was performed with Ni-NTA column and then size exclusion column chromatography. Expression and purification levels of poly lysine tagged scFv lym-1 antibody were confirmed by western blot analysis. I-124, I-125, I-131 and Tc-99m were used for radiolabeling of purified poly lysine scFv lym-1. Flow cytometry analysis of FIT( conjugated poly lysine scFv lym-1 was performed for confirmation of immunoreactivity of human Burkitt's lymphoma cells. Results: Poly lysine scFv lym-1 antibody was purified through two steps and identified as molecular weight of 48 KDa. Radiolabeling yields of I-124, I-125, I-131 and Tc-99m into poly lysine scFv lym-1 were >99%, >99%, >95% and >99%, respectively. Flow cytometry analysis of poly lysine scFv and scFv lym-1 was showed similar immunoreactivity to human Burkitt's lymphoma cells. Conclusion: Poly lysine tag was useful for the sufficient number of amino groups to scFv lym-1 antibody for chelator conjugation with minimizing loss of immunoreactivity.

• Journal of the Society of Disaster Information
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• v.17 no.1
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• pp.10-24
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• 2021

Purpose: The purpose of this study is to determine the dangers of biodiesel and general diesel mixtures currently used as alternative fuels by equipment (tag method and penski Marten method) and to determine the difference between flash point and combustion point (closed, open) according to test methods. It is intended to be used as a reference material for identification and evaluation of firecausing substances by confirming the risk of mixtures by comparative analysis and measurement, and establishing a risk assessment method for chemical substances. Method: Flash point test method and result treatment were tested based on ASTM and KS M mode, which are tag sealing and pen schematense test methods used as flash point and combustion point test methods for crude oil and petroleum products. The manufacturer of the equipment used in this experiment was a test equipment that satisfies the test standards of KS M 2010 with equipment produced by TANAKA of Japan. The flash point and combustion point were measured, and the flash point according to the test method of biodiesel and general diesel mixture ( Closed, open), and the ignition point of a mixture of biodiesel and general diesel was compared and analyzed for ignition risk compared with conventional diesel. Results: Looking at the experimental results, first, as an analysis of the risk of flammability of the mixture, the flash point of a substance containing 70% biodiesel was found to be about 92℃ based on general diesel with a flash point of 64.5℃, and gasoline and biodiesel or When the biodiesel mixture was synthesized, it was confirmed that the flash point tends to decrease. In addition, the difference between the flash point and the combustion point was analyzed as about 20 ~ 30℃, and when a small amount of gasoline or methanol was mixed, the flash point was lowered, but it was confirmed that the combustion point was similar to that of the existing mixture. Conclusion: In this study, in order to secure the effectiveness of the details of the criteria for judging dangerous materials in the existing Dangerous Materials Safety Management Act, and to secure the reliability and reproducibility of the judgment of dangerous materials, we confirm the criteria for judging the risk of the mixture through an experimental study on flammable mixtures. It will be able to provide reference data for experimental criteria for flammable liquids that are regulated in the field. In addition, if this study accumulates know-how on experiment by test method, it is expected that it can be used as a basis for research on risk assessment and research on dangerous goods.