• 대학교육
• /
• s.97
• /
• pp.118-123
• /
• 1999

• KOREAN POULTRY JOURNAL
• /
• v.23 no.1 s.255
• /
• pp.44-45
• /
• 1991

• 사료
• /
• s.11
• /
• pp.58-64
• /
• 2004

• 사료
• /
• s.27
• /
• pp.50-55
• /
• 2007

• 방재와보험
• /
• s.32
• /
• pp.24-26
• /
• 1987

• 건강소식
• /
• v.17 no.5 s.174
• /
• pp.32-34
• /
• 1993

• 사료
• /
• s.14
• /
• pp.52-60
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• 2005

• Korean journal of applied entomology
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• v.30 no.4
• /
• pp.258-264
• /
• 1991

Studies were carried out to investigate effects of temperature and photoperiod on diapause induction and of juvenile hormone III and 20-hydroxyecdysone treatment on diapausing adults of the alder leaf beetle, Agelastica coerulea Baly(Chrγsomelidae: Coleoptera). Its life cycle and ovarian development in adults were also observed. The beetle had one year life cycle with egg, larval, pupal and adult periods being 7-10, 19-21, 14-15 days and about 10 months, r respectively. All adults showed a diapause syndrome when the larvae were reared at $20^{\circ}C$ or $25^{\circ}C$ in combination of photoperiods of 16L/8D, 12L/12D, or 8L/16D. Their ovarioles did not s show any development of vitellogenesis before or during diapause and even when exposed at $15^{\circ}C$ after overwintering. When diapausing adults were treated with JH III they resumed feeding and laid several eggs and broke diapause condition temporally. But diapausing adults treated with 20-hydroxyecdysone did not show any response.

• The Korean Journal of Pesticide Science
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• v.18 no.3
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• pp.202-209
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• 2014

RNA interference (RNAi) is the method which controls phenotypes of gene in live cells. Chitinase is the enzyme helping digestion and absorption of old cuticles during the ecdysis of insects. In order to investigate molting-inhibition effect with the chitinase related gene in Spodoptera litura, RNA was extracted from the $5^{th}$ instars. cDNA was synthesized and then we obtained about 700 bp size chitinase. After PCR products were cloned into a pGEM T-easy vector, colonies were picked. DNA was extracted from the colony cultures. EcoR I enzyme was used to check whether PCR products were inserted or not. And then we confirmed vector band of about 3 kb and insert band of about 700 bp. To synthesize the dsRNA, each DNA was cut with Spe I and Nco I enzymes (Circular DNA became lineared DNA). After synthesis of dsRNA, approximately 5 ul dsRNA was injected into the $3^{rd}$ abdominal segment of S. litura $4^{th}$ larvae. The concentration of dsRNA was about $10{\mu}g/{\mu}l$. We confirmed larval-larval molting : there were phenotypically abnormal individuals - for instance malformation, molting inhibition and change of integument color. Pupaadult molting : there were phenotypically abnormal individuals - for instance molting inhibition, change of wings and malformation. Also we could investigate the pupation, emergence and variation about noninjection, treated with DW and dsRNA. Each pupation was non-injection 83.3%, DW 78.3% and dsRNA 66.7%. Each emergence was non-injection 90.0%, DW 72.3% and dsRNA 65.0%. So we considered that chitinase dsRNA induced molting inhibition effect. But each variation was non-injection 8.9%, DW 2.9% and dsRNA 19.2%. Therefore dsRNA group showed the highest variation value. When 18 hours after injecting dsRNA, we could obtain abnormal individual.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.08a
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• pp.92-92
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• 2010

전북대학교에서는 우리나라 최초로 0.4 및 2.4 MW 급 초음속 열플라즈마 시험 시설 구축사업을 진행하고 있으며, 이를 이용한 응용 분야 별 선행연구를 수행하고 있다. 구축 시험 시설의 핵심장치인 MW 급 대출력 초음속 열플라즈마 발생기로는 양극과 음극 사이에, 전기적으로 절연된 도넛 형태의 간극을 다수 삽입하여 아크 길이를 늘림으로써, 플라즈마 출력을 비례하여 높일 수 있는 Segmented 형 아크 직류 토치를 사용하고자 하며, 제작을 위해 설계 중인 토치는 0.4 및 2.4 MW 출력에 대해, 마하 2 이상의 초음속 유동에서 각각 13 및 20 MJ/kg 이상의 플라즈마 비엔탈피 구현을 목표로 하고 있다. 특히, 이와 같은 고엔탈피 초음속 유동의 달성은 0.4MW 급의 경우엔 공기유량 0.01 kg/s 이상에서, 2.4 MW 급의 경우엔 0.05 kg/s 이상에서 10Torr 이하의 진공과 투입된 MW 규모의 열량을 지속적으로 유지 및 제거할 수 있는 시설이 있어야 구현 가능하므로 이를 위한 건축과 지원시설 구축을 동시에 진행하고 있다. 본 발표에서는 0.4 MW 급 초음속 열플라즈마 시험 시설을 중심으로, 상기 MW 급 Segmented 형 아크 직류토치와 이를 구동하기 위한 대출력 초음속 열플라즈마 시험 시설에 대해 그 동안 전북대학교에서 진행되어 온 개념설계 내용을 소개하고자 한다. 덧붙여, 최근 본 사업단에서 선행 연구 중인 고엔탈피 초음속 열플라즈마 진단 계측 기법과 향후 응용분야 및 핵심 연구개발 과제 등에 대한 간략한 소개도 함께 하고자 한다.