• Journal of Life Science
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• v.13 no.4
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• pp.511-515
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• 2003

White rot fungi, Phanerochaete sp. EJ-31L, was evaluated for its ability to decolorize Remazol Black B, an azo dye that is a widespread pollutant in the wastewater of textile industry. It was observed that extent of decolorization by Phanerochaete sp. EJ-31L was dependent on the concentrations of co-carbon and nitrogen source. Effects of agitation and aeration were studied, and agitated culture at aeration condition resulted in greater extent of decolorization than static culture. Remazol Black B was readily decolorized up to 95% within 64 hr by Phanerochaete sp. EJ-31L.

• Korean Journal of Microbiology
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• v.33 no.1
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• pp.43-48
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• 1997

여러가지 난분해성 물질에 대한 생분해능을 지닌 백색부후균에 의한 매립지 침출수의 탈색을 조사하였다. 국내에서 분리한 Coriolus versicolor KR-11W와 Irpex lacteus KR-39W가 이제까지 주로 연구되어 온 Phanerochaete chrysosporium보다 높은 탈색능을 나타내었는데 I. lacteus KR-39W는 산소공급시 10%의 침출수가 함유된 YMG 배지의 진탕배양에서 85%의 색도제거율을 나타내었으며 최소배지에서도 80%의 탈색율을 보였다. P. chrysosporium에 의한 리그닌 분해능 및 분해효소 생성 보고들과 달리 진탕배양이 정치배양보다 탈색능이 높았으며 산소공급은 색도제거에 증가효과가 있었다. 균체 접종량(10-30%)과 온도(25,37.deg. C)는 탈색에 큰 차이를 보이지 않았으나 탄소원과 질소원의 농도는 상당한 영향을 나타내었다. 리그닌 분해효소군의 여러 가지 inducer와 cofactor를 C. versicolor KR-11W 배양에 첨가한 결과 많은 경우 균접종 대조군보다 2배 이상의 탈색율과 lignin peroxidase 활성의 증가를 보였으며 FeS $O_{5}$ 첨가시에는 최대 2.9배의 증가를 나타내었다. 탈색에 관여하는 효소군은 접종물에 이미 어느 정도 존재할 수 있으며 배지 및 배양조건에 따라 그 생성이 변화할 수 있으므로 이런 조건들을 잘 맞출 경우 보다 높은 탈색능이 기대된다.

• The Korean Journal of Mycology
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• v.42 no.3
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• pp.213-218
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• 2014

Water extract from spent mushroom substrates (SMS) of Pleurotus eryngii was utilized in decolorization of eight synthetic dyes and wastewater from a textile factory. High laccase activity was detected in the extract of P. eryngii (SMSE). The SMSE showed that decolorization rate was 34~93% after 24 h incubation without any mediator on eight dyes including Rit-blue and Rit-red used in fiber dyeing. Dye decolorization rate more than 90% was observed on bromophenol blue and remazol brilliant blue R (RBBR). Dye in textile wastewater was decolorized at room temperature after three days by addition of P. eryngii SMSE. The results suggest that biological decolorization of dyes using the P. eryngii SMSE can be used as environmental friendly materials.

• Applied Chemistry for Engineering
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• v.32 no.1
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• pp.61-67
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• 2021

In this study, the adsorption decolorization process of sea buckthorn oil was carried out to verify the possibility of the sea buckthorn oil as a natural UV absorber. The optimization was carried out by using the central composite design model-response surface methodology (CCD-RSM). The response values of CCD-RSM were selected as the decolorization effect through the process, acid value after decolorization, and UV absorbance of the decolored oil at 290nm. The amount of adsorbent, temperature and time were selected as the process variables for the experiments. According to the results of CCD-RSM, the results of optimization were all consistent. The optimal conditions, which satisfy CCD-RSM statically and mathematically, were 4.32 wt.%, 134.90 ℃, and 19.8 min for the amount of adsorbent, temperature and time, respectively. The estimated response values expected under these optimal conditions values were 94.78%, 2.08 mg/g KOH, and 2.91 for the decolorization effect, acid value and UV absorbance at 290 nm, respectively. Also the average error from actual experiment for verifying the conclusions was smaller than 2%. Therefore, it was confirmed that the application of CCD-RSM to the adsorption decolorization process of sea buckthorn oil showed a very high level of acceptable results and that the sea buckthorn oil has high possibility to be used as a natural UV absorber.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.777-784
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• 1993

The bleaching effect of potato lipoxygenase Isoenzymes on ${\beta}-carotene$ was studied. Two lipoxygenase Isoenzymes(LOX-1, LOX-2) from potato tuber were purified by CM-cellulose, DEAE-cellulose ion exchange chromatography. LOX-1 and LOX-2 seemed to have bleaching effect on ${\beta}-carotene$ in the presence of linoleic acid, which the decrease in the formation of conjugated dienes. LOX-2 was founded to have a greater pigment bleaching activity than that of LOX-1.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.1
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• pp.47-52
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• 1989

Aspergillus awamori, B-2 which has a high ability to decolorize melanoidin was selected among various fungi. Aspergillus awamori, B-2 showed the highest decolorization activity when it was cultivated in a melanoidin medium containing 3.0% glucose, 0.5% yeast extract, 0.1 % $KH_2PO_4\;and\;0.05%\;MgSO_4{\cdot}7H_2O$ at an initial pH 7.0 at $37^{\circ}C$ for 5 days. Mycelia immobilized system with. Ca-alginate was more effective on melanoidin decolorization activity showed approximately 70% in 10 days under the optimal conditions. Continuous decolorization of melanoidin using, reuse of immobilized mycelia showed an almost constant decolorization of abort 60-70% for 15 days.

• Korean Journal of Microbiology
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• v.33 no.4
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• pp.252-256
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• 1997

The white rot fungus Coriolus hiysutus decolorized several recalcitrant dyes. Four different types of dyes, including azo, triphenyl methane, heterocyclic, and polymeric dye, were treated by the mycelial preparation. Triphenyl methane dye, bromophenol blue lost over 95% of its color. Congo red and Poly R-478 were decolorized less than bromophenol blue, 57 and 55%, respectively. However, heterocyclic dye, methylene blue was not decolorized significantly and only red shift was observed. Extracellular laccase and peroxidase activities were appeared maximally in high level of dye decolorization media. In electrophoretic experiments, common active bands of laccase and peroxidase were found in all dye decolorized medium. These results indicated that the culture conditions which yield high levels of laccase and peroxidase activity lead to high levels of dye decolorization, and these two enzymes might be play an important roles in dye decolorization.

• Microbiology and Biotechnology Letters
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• v.26 no.3
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• pp.269-274
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• 1998

Decolorizing characteristics of crystal violet by Enterobacter cloace MG82, which can decolorize rapidly triphenylmethane dyes, were investigated. The higher growth and decolorization activity was shown at big ratio of dissolved oxygen in the medium. The decolorization activity of crystal violet revealed highest at the middle of lag phase. As the concentration of crystal violet was higher, the growth of E. cloacae MG82 and decolorizing activity of crystal violet by this strain were worse. The maximum concentration of crystal violet at which E. cloacae MG82 be able to grow was 375 ${\mu}$M. E. cloacae MG82 was not able to use the crystal violet itself as a sole carbon source. So, it was shown that growth of E. cloacae MG82 and decolorization activity of crystal violet by this strain needed addition of another energy sources except this dye.

• The Journal of Natural Sciences
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• v.9 no.1
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• pp.9-17
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• 1997

We studied on the decoloring efficiency of a dying and finishing industrial wastewater containing reactive dyes in the ozone treatment system. The wastewater containing reactive dyes improved the decolorization by the ozone oxidation. In order to determine the optical conditions for ozonation, various operation factors such as initial wastewater concentrations, pH, temperature, and ozone dose were evaluated. Ozone utilization rate was improved in general with the increase of initial concentration of wastewater. And the decolorization speed or reactive dyes was more decreased in deep color of blue series than in light color such as yellow series. The colorization of wastewater containing reactive dyes was changed step through midle color depending upon increasing of ozone treated time. The efficiency of decolorization for wastewater was improved with the pH increased and the temperature of ozone treatment system decreased.

• Journal of Life Science
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• v.14 no.4
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• pp.577-581
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• 2004

Comamonas sp. AEBL-85 was isolated from microbial granules in an activated sludge process of long-term operated for the treatment of reactive azo dye, and characterized its capability to decolorize Reactive Black 5. The effects of adding carbon source and nitrogen source on the extent of decol-orization were analyzed to develop an optimal medium. The optimum initial pH and temperature wire 6.0 and 35$^{\circ}C$, respectively. Reactive Black 5 of 50 mg/l was readily decolorized up to 95% within 40 hr by Comamonas sp. AEBL-85.