• Title/Summary/Keyword: 크로마토그래피

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Studies on Simultaneous Analysis of Organophosphorus Pesticide Residues in Crops by Gas-Liquid Chromatography (I) Extraction and Cleanup (기체-액체 크로마토그래피에 의한 농작물 중 유기인제 잔류 농약의 동시 분석에 관한 연구 (제 1 보). 용매추출 및 방해성분의 분리 제거)

  • Taek-Jae Kim;Yun-Woo Eo;Young Sang Kim
    • Journal of the Korean Chemical Society
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    • v.30 no.5
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    • pp.465-474
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    • 1986
  • The solvent extraction and cleanup processes for the simultaneous gas-liquid chromatographic determination of 11 kinds of organophosphorous pesticide residues in crops were investigated. The extracts dissolved with acetone were partitioned with petroleum ether after adding saturated NaCl solution. Evaporated the partitioning solvent, the residue was dissolved in methylene chloride and eluted through mixed adsorbent (1 : 2 : 4 of activated carbon, magnesia and diatomaceous earth) with methylene chloride as an eluent. The pesticides recovered were 82∼105% and the impurities were effectively removed.

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Synthesis of O-Diethylaminoethyl Chitosan for Chromatography; I. Separation of Protein (크로마토그래피용 O-Diethylaminoethyl Chitosan의 합성; I. 단백질의 분리)

  • Park, Chang-Gu;Lee, Young-Moo;Kim, Jin-Hong;Jeong, Chang-Nam
    • Applied Chemistry for Engineering
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    • v.4 no.3
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    • pp.583-590
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    • 1993
  • O-Diethylaminoethyl chitosan containing weak cation exchange group had been synthesized. It was used to separate albumin and lysozyme as stationary phase in liquid chromatography column. The separation of acidic and basic dyes were preliminarily conducted to investigate the effect of polarity parameter of solvents. Based on this result, we investigated various factors on the separation efficiency of chromatography in column. In the case of using water-methanol as cosolvent, the resolution factor depended on the polarity parameter. The best resolution factor was 1.42 for DEAE-chitosan with the degree of substitution of 0.23.

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Use of HCI Program for Optimization of Operating Conditions in Analytical and Preparative Chromatography (분석 및 분리용 크로마토그래피에서 조업조건의 최적화를 위한 HCI 프로그램의 이용)

  • Lee, Ju-Won;Lee, Min-U;No, Gyeong-Ho
    • KSBB Journal
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    • v.14 no.4
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    • pp.408-413
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    • 1999
  • To separate mixtures analytically and preparatively by LC (Liquid Chormatography), the operating conditions of analytical chromatography should be determined. The HCI program was utilized to find the optimum operating condition accurately and rapidly, and to reduce the number of experiments. In an analytical chromatography, based on the resolution and analysis time, the experimental conditons of deoxyribonucleosides and phopholipids were fixed in terms of taxol was calculated, and the collection time was predicted for the mixture of 5'-IMP and 5'-GMP from the elution profile when and purity wer known.

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