• Title/Summary/Keyword: 콜라겐 합성

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Effect of Vitamin C, Silicon and Iron on Collagen Synthesis and Break-Down Enzyme Expression in the Human Dermal Fibroblast Cell (HS27) (피부 섬유아세포에서 비타민 C, Silicon, 철분 처리가 콜라겐 합성 및 분해 관련 효소의 발현에 미치는 효과 비교)

  • Kim, Jeong-Eun;Lee, Jin-Ah;Kim, Hyun-Ae;Kim, Jung-Min;Cho, Yun-Hi
    • Journal of Nutrition and Health
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    • v.42 no.6
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    • pp.505-515
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    • 2009
  • Collagen is the major matrix protein in dermis and consists of proline and lysine, which are hydroxylated by prolyl hydroxylase (PH) and lysyl hydroxylase (LH) with cofactors such as vitamin C, oxygen, iron (Fe$^{2+}$), ketoglutarate and silicon. The collagen degradation is regulated by matrix metalloproteinase-1 (MMP-1), of which is the major collagen-degrading proteinase whereas tissue inhibitors of metalloproteinase-1 (TIMP-1) bind to MMP-1 thereby inhibiting MMP-1 activity. In this study, we investigated the effects of vitamin C, silicon and iron on mRNA, protein expressions of PH, LH, MMP-1 and TIMP-1. The physiological concentrations of vitamin C (0-100 $\mu$M), silicon (0-50 $\mu$M) and iron (Fe$^{2+}$:0-50 $\mu$M) were treated to human dermal fibroblast cells (HS27 cells) for 3 or 5days. The expression level of mRNA and protein was increased in not only PH but also LH when cells were incubated with vitamin C. A similar increase in LH mRNA or protein expression occurred when cells were incubated with silicon. Our results suggest that treatment of vitamin C and silicon increased mRNA and protein expression of PH and LH in human dermal fibroblast.

Constituents of Collagen Synthesis Activation from the Extracts of Gynostemma pentaphyllum Leaves (돌외 잎 추출물의 콜라겐 합성 증진 성분 규명)

  • Yim, Jun Hwan;Jang, Moon Sik;Jung, Uk Sun;Moon, Mi Yeon;Lee, Ha Youn;Kim, Young Hoon;Lee, Gi Yong;Lee, Nam Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.3
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    • pp.289-295
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    • 2014
  • In order to discover ingredients for wrinkle-care cosmetics, we prepared 70% ethanol extract from Gynostemma pentaphyllum and examined its activity on collagen synthesis using fibroblast HDFn cells. The G. pentaphyllum extract induced the production of type I procollagen in a dose-dependent manner without showing cell toxicity. The active constituents were isolated from the extract by solvent fractionation and chromatographic purification procedures. NMR data and literature studies led to determine the two isolated compounds as the flavonoid glycosides such as ombuine 3-O-rutinoside (1) and quercetin 3-O-rutinoside (2). The activity screening tests showed that the isolates 1 and 2 induced the production of type I procollagen in a dose-dependent manner. These results suggested that G. pentaphyllum extract containing the flavonoids 1 and 2 could be useful as an active ingredient for wrinkle-care cosmetics.

Effect of L-Ascorbic Acid on Collagen Synthesis in 3T6 Fibroblasts and Primary Cultured Cells of Chondrocytes (3T6 세포주 및 연골 초대배양세포의 Collagen 합성에 미치는 비타민 C의 영향)

  • Kim, Mi-Hyang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.1
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    • pp.42-47
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    • 2006
  • L-Ascorbic acid (AsA) is an essential nutrient for prevention of scurvy in humans, primates and guinea pigs that lack $L-gulono-\gamma-lactone$ oxidase which is required for the final step of AsA biosynthesis. AsA participates in various hydroxylation reactions involved in the biosynthesis of collagen. The purpose of this study is to clarify the role of AsA on collagen synthesis in 3T6 fibroblasts and primary cultured cells of chondrocytes. Cells were cultured in medium supplemented with catalase and AsA at various concentration. Supplement of AsA induced collagen synthesis in 3T6 fibroblasts and primary cultured cells of chondrocytes. The most remarkable induction of collagen synthesis by AsA was found in primary cultured chondrocytes. The content of collagen representing the amounts of extracellular matrix significantly increased in the cells of which growth was stimulated by AsA, while it decreased with increasing passage numbers of subculture in cells. It showed that the content of collagen decreased in the medium which contained AsA at the concentration higher than 5.0 mM. However, the contents of collagen to DNA were not different among various AsA concentrations. Supplementing with AsA resulted in enhancement of collagen formation and extracellular matrix. Therefore, there might be a Positive correlation between the activity of catalase and the AsA concentration. Moreover, it can be assumed that AsA stimulates the collagen synthesis by optimizing the cell-culture environment.

Effects of Albizziae Cortex Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity (합환피(合歡皮) 약침액(藥鍼液)의 사람 피부아세포의 콜라게나제 활성 및 프로콜라겐 합성과 티로시나제 활성에 미치는 영향)

  • Leem, Kang-Hyun
    • Journal of Acupuncture Research
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    • v.28 no.2
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    • pp.125-131
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    • 2011
  • 목적 : 본 연구는 합환피약침액(合歡皮藥鍼液)이 사람 피부 섬유아세포의 콜라게나제 활성 및 프로콜라겐 합성에 미치는 영항과 티로시나제 활성에 미치는 효과를 측정하고자 실시하였다. 방법 : HS68 사람 정상 섬유아세포에 UVB 조사 후 합환피(合歡皮) 약침액(藥鍼液)가 type I procollagen 생성과 콜라게나제 효소활성에 미치는 효능과 티로시나제 효소활성에 미치는 효능을 평가하였다. 결과 : 합환피약침액(合歡皮藥鍼液)은 UVB 조사된 세포의 콜라게나제 효소활성을 통계적으로 유의하게 억제하였고, 티로시나제 활성을 통계적으로 유의하게 억제하였다. 그러나 티로시나제 억제활성의 정도는 미백효능으로 활용하기에 약간 약한 경향이 있었다. 결론 : 합환피약침액(合歡皮藥鍼液)의 콜라게나제 억제효능은 주름개선 약침치료에 활용이 가능할 것으로 생각된다.

Changes of Facial Wrinkle after Topical Application of On Emulsion Containing Medimin A (Medimin A를 함유한 O/W 에멀전의 주름 개선 효과)

  • 박선규;장민열;김영득;정봉열;원영호;김진준;강세훈
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.25 no.1
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    • pp.23-36
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    • 1999
  • Medimin A is a derivative of vitamin A which has been developed by coupling retinoic acid with polyethylene glycol(PEG) to enhance skin permeability and stability. We carried out the collagen synthesis and clinical test to examine the reducing effect of wrinkles by Medimin A. In vitro collagen synthesis was evaluated by quantitative assay of ($^3$H)-proline incorporation into collagenase sensitive protein in fibroblast cultures. Clinical test was evaluated by image analysis of skin replica, visual observation and self-estimated response of volunteers for 10 weeks. Medimin A stimulated about 40% in collagen synthesis. The area of main deep wrinkle on the skin replica was reduced 38.4% with topical application of O/W emulsion containing 0.2% Medimin A. The wrinkles on the eye region was also reduced 25.4%-44.1% by the visual observation and 93% of all volunteers responded that topical application of the O/W emulsion was showed some reducing effect of wrinkles after 10 weeks. From these results, we suggest that Medimin A is a potent anti-wrinkle agent by objective evaluation methods(in vitro collagen synthesis and image analysis of skin replica) and subjective evaluation methods(visual observation and self-estimated response of volunteers).

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The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts (제니스테인에 의한 노화된 피부세포 활성화와 콜라겐 생성 효과)

  • Yang, Eun-Soon;Hong, Ran-Hi;Kang, Sang-Mo
    • Microbiology and Biotechnology Letters
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    • v.35 no.4
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    • pp.316-324
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    • 2007
  • We studied the effects of genistein obtained from glycolysis of genistin, a kind of phytoestrogen present in soybeans, on cell proliferation and type I pN collagen synthesis in normal human dermal fibroblasts(NHDF). Cell proliferation was increased significantly with genistein treatment at 54-year aged NHDF. Genistein increased cell proliferation more strongly in cells form old doner than young doner. The senescence-associated ${\beta}$-galatosidase activity was decreased in NHDF from 77-year old doner with genistein treatment. Type I pN collagen synthesis was increased with genistein treatement in UVA treated and non-treated NHDF. The increasement of collagen synthesis was more effective in aged cells than young cells. Type I pN collagen synthesis was also increased with genistein treatment in collagen matrix culture with NHDF from sun-exposed and non-exposed skin from 54-year old doner. Genistein treatment inhibited MMP-1 synthesis in old NHDF but not in young NHDF. In conclusion, genistein may be a useful agent for preventing intrinsic aging as well as photoaging.

Effects of Yukmigeehwang-hwan Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity (육미지황환(六味地黃丸) 약침액(藥鍼液)의 사람 피부아세포의 콜라게나제 활성 및 프로콜라겐 합성과 티로시나제 활성에 미치는 영향)

  • Lee, Se-Na;Kim, Myung-Gyou;Kim, Myoung-Hee;Kim, Hyung-Jun;Jo, Hak-Jun;Kim, Ee-Hwa;Leem, Kang-Hyun
    • Korean Journal of Acupuncture
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    • v.28 no.1
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    • pp.39-46
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    • 2011
  • 본 연구는 육미지황환(六味地黃丸) 약침액(藥鍼液)가 사람 피부 섬유아세포의 콜라게나제 활성 및 프로 콜라겐 합성에 미치는 영항과 티로시나제 활성에 미치는 효과를 측정하고자 실시하였다. 방법 : HS68 사람 정상 섬유아세포에 UVB 조사 후 육미지황환(六味地黃丸) 약침액(藥鍼液)가 type I procollagen 생성과 콜라게나제 효소활성에 미치는 효능과 티로시나제 효소활성에 미치는 효능을 평가하였다. 결과 : 육미지황환(六味地黃丸) 약침액(藥鍼液)은 UVB 조사된 세포의 콜라게나제 효소활성을 통계적으로 유의하게 억제하였고, 티로시나제 활성과 L-DOPA oxidation 활성 또한 통계적으로 유의하게 억제하였다. 그러나 티로시나제와 L-DOPA oxidation 억제활성의 정도는 미약하였다. 결론 : 육미지황환(六味地黃丸) 약침액(藥鍼液)의 콜라게나제 억제효능은 주름개선 약침 치료에 활용이 가능할 것으로 생각된다.

Effect of Pine (Pinus densiflora) Needle Extracts on Synthesis of Collagen in Osteoblastic MC3T3-E1 Cells (적송잎 추출물이 조골세포의 collagen 합성에 미치는 영향)

  • Jeon, Min-Hee;Kim, Young-Kyoung;Park, Yong-Soo;Hwang, Hyun-Jung;Kim, Sung-Gu;Lee, Sang-Hyeon;Choi, In-Soon;Kim, Mi-Hyang
    • Journal of Life Science
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    • v.20 no.4
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    • pp.607-613
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    • 2010
  • Osteoporosis is a disease involving a decrease in bone mineral density and an increased risk of fractures. The MC3T3-E1 pre-osteoblastic cell line is a well-accepted model of osteogenesis in vitro. Pine needles have long been used as a traditional health-promoting medicinal food in Korea. In this study, MTT assay, the alkaline phosphatase (ALP) activity and collagen synthesis of osteoblast cells were investigated to determine the effects of pine needle extracts on cell proliferation and differentiation. Pine needle extracts were prepared using hexane, ethanol and water. The effects of the pine needle extracts were examined by comparing the results with those of commercial agents, such as proanthocyanidin. The MC3T3-E1 cells exposed to proanthocyanidin showed increased proliferation in a concentration-dependent manner. The cells exposed to the hexane extract showed a similar increase in proliferation to that observed with proanthocyanidin. The hexane extract showed the highest ALP activity. Moreover, a supplement of pine needle extracts induced collagen synthesis in MC3T3-E1 cells. The pine needle extract produced the highest level of collagen synthesis at concentrations of $10{\sim}50\;{\mu}g/ml$. These results indicate that pine needle extracts have an anabolic effect on bone by promoting osteoblastic differentiation, and may be used in the treatment of common metabolic bone diseases.

Physiological Activities of Cudrania tricuspidata Extracts on the Skin (꾸지뽕나무 추출물의 피부 생리 활성)

  • Choi, Hak Joo;Kim, Cheong Taek;Do, Min Yeon;Rang, Moon Jeong
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.260-274
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    • 2015
  • This paper has shown the experimental results about the physiological activities of water-, ethanol-, ethyl acetate-soluble fractions from ethanolic extracts of leaves, stems and roots of Cudrania tricuspidata on the skin, which has been used for a long time as a traditional herb medicine in Korea and China. The effects of these fractions on the secretion of nitric oxide and cytokines from macrophage(RAW 264.7 cell) exhibited that the ethyl acetate and water fractions from leaves inhibited the release of nitric oxide, all fractions inhibited thoses of inflammatory cytokine $IL-1{\alpha}$, and the ethyl acetate fractions of leaves, stems and roots inhibited thoses of inflammatory cytokine IL-6. The only ethylacetate fraction of leaves demonstrated significantly the reduction of melanin synthesis in melanoma cells. In order to evaluate the efficacy of collagen synthesis, the treatment with extracts on the human normal fibroblast cell(CCD-986sk cell) resulted in finding that the water fractions of leaves, stems and roots and the ethanol fractions of leaves and stems showed the increased synthesis of collagen.

Physiological Functions of Lactic Acid Bacteria Fermented Broth Containing Fagopyrum esculentum and Saccharina japonica (메밀 및 다시마를 포함하는 유산균 발효액의 생리적 기능)

  • Jeon, Sung-Jong;Kim, Ae-Ryoung;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.25 no.10
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    • pp.1110-1114
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    • 2015
  • In this study, we investigated the potential of Lactobacillus brevis AR1 fermented broth containing various grains (Fagopyrum esculentum, Scotch oat, Sesamum indicum, Glycine max Merr, Castanea crenata, Oryza sativa L., Hordeum vulgare L., Perilla frutescens var. japonica Hara, or Triticum aestivum L.) or Saccharina japonica as a source of collagen synthesis in cosmetic products. The treatment of Lb. brevis AR1 fermented broths containing F. esculentum or S. japonica water extracts was markedly increased the synthesis of collagen in fibroblasts. The collagen synthesis capacity of the S. japonica fermentation product was higher than that of β-glucan, which was used as a positive control. Under controlled conditions in broths containing F. esculentum or the S. japonica extracts with 4% monosodium glutamate (MSG), Lb. brevis AR1 produced γ-aminobutyric acid (GABA) at a concentration of 180 mM, with an 84.5% GABA conversion rate after 72 h. Both the F. esculentum and S. japonica fermentation broths produced by Lb. brevis AR1 reduced inflammatory responses on mouse skin and did not show cell cytotoxicity in fibroblasts. These results suggest that both the F. esculentum and S. japonica fermentation products of Lb. brevis AR1 could be used as functional materials in cosmetic products to combat wrinkles and skin inflammation.