• Journal of the korean academy of Pediatric Dentistry
• /
• v.38 no.3
• /
• pp.296-302
• /
• 2011

Idiopathic gingival fibromatosisrarely occurs, but frequently recurred after surgical removal. It usually occurs in generalized symmetrical pattern but sometimes in localized unilateral pattern. The localized pattern usually affects the maxillary molar and tuberosity area. This disease usually causes tooth migration, malocclusion, and problems in eating, speech, and esthetics. A boy showed dense gingival fibromatosis localized at primary maxillary right lateral incisor area at the age of 5 years, and his maxillary right lateral incisor become severely displaced at the age of 9 years. He had no medical and hereditary factors relevant to the gingival fibromatosis. However, the dense fibrous tissue was dominant in his labial gingiva of maxillary right incisors. In order to realign the displaced incisors by orthodontic treatment, the dense fibrous tissue covered the defect space between the central incisor and the displaced lateral incisor was surgically removed. The removed specimen was examined by simple immunohistochemical(IHC) array method. IHC array showed increased expression of CTGF, HSP-70, MMP-1, PCNA, CMG2, and TNF-${\alpha}$ in keratinocytes, fibroblasts, endothelial cells, and macrophages of gingival fibromatosis tissue. Therefore, it was suggested that the gingival fibromatosis be caused by the concomitant overexpression of CTGF, HSP-70, MMP-1, PCNA, CMG2, and TNF-${\alpha}$, and resulted in the fibroepithelial proliferation and the inflammatory reaction of gingival tissue.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.35 no.1
• /
• pp.30-38
• /
• 2008

Dental pulp cells are assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. The purpose of this study is to examine the effects of mineral trioxide aggregate (MTA) on various gene expression regarding dentinogenesis and cell viability assay in cultured primary human dental pulp cells. The author also examined the effects of this material on cellular alkaline phosphatase activity as a potential indicator of dentinogenesis. For gene expression on MTA, reverse transcriptase polymerase chain reaction was performed using primer sets of glyceraldehyde-3-phosphate dehydrogenase, type I collagen, alkaline phosphatase(ALP), osteonectin, and dentin sialoprotein after 2 and 4 days. Cell viability assay showed that the proportion of MTA-treated pulp cells which had been exposed for 5 days to MTA was higher than that of the control cells. Among the genes investigated in this study, ALP and osteonectin(SPARC) were increased in MTA treated group than in control. These findings suggest that this dental pulp culture system may be useful in the future as a model for studying the mechanisms underlying dentin regeneration after the treatment with MTA. Exposure to MTA material would not induce cytotoxic response in the dental pulp cells. In addition, MTA could influence the behavior of human pulp cells by increasing the ALP activity and SPARC synthesis.

• The korean journal of orthodontics
• /
• v.35 no.2 s.109
• /
• pp.106-115
• /
• 2005

This study was aimed to investigate the effects of indomethancin on physiologic root resorption and to examine the dental pulp and tissue changes around the resorbing teeth 13-14 week old six mongrel dogs were divided into 3 groups, two experimental groups administered indomethacin 2mg/kg/day and 8mg/kg/day orally two times a day for 14 days respectively. and control group administered a placebo The deciduous incisors showing root resorption were selected. fixed for 24 hrs in $10\%$ formalin solution. demineralized in $10\%$ EDTA solution. Invested in paraffin and sectioned in $5{\mu}m$ thick sections. The preparations were stained with H&E staining and Masson's trichrome staining and examined under the light microscope Observation revealed that deciduous root resorbing tissue resembles inflammatory tissue and accompanies bore remodelling. The dental pulp was formal except the area near root resorption. well organized columnar odontoblasts layer under the predentin, anud the odontoblasts near root resorption were cuboidal or flat cells in the disrupted layer under the predentin. Indomethacin administered group showed a partial decrease in the number of odontoclasts and nucleus But there was no sign of pulp change by indomethacin. These results suggest that indomethacin inhibits recruitment of odontoclasts partially and that of osteoclasts more. and so when it is administered for long periods deciduous root resorption can be delayed and eruption of the successor can be delayed for a short period.

• The korean journal of orthodontics
• /
• v.36 no.4
• /
• pp.242-250
• /
• 2006

Objective: Periodontal ligament fibroblasts have an ectomesenchymal origin and are thought to play a crucial role for not only homeostasis of periodontal tissues but also bone remodeling, wound healing and regeneration of tissues. Recently, it has been reported that UNC-50 is not expressed in gingival fibroblasts but in PDL fibroblasts. The purpose of this study was to examine the expression of UNC-50 and osteocalcin in the periodontium after application of intermittent force. Methods: Twelve rats had 40 grams of mesially-directed force applied at the upper molar for 1 hour/day. Four rats were sacrificed at 1, 3 and 5 days. Immunohistochemical localization of UNC-50 and osteocalcin antibody was carried out. The results showed apposition of new cellular cementum and a slight increase in periodontal space at the tension side. Results: Strong UNC-50 expression was observed in the differentiating cementoblasts close to PDL fibroblasts in the tension side whereas it was barely expressed at the compression side. Expression was strong at day 3, and decreased at day 5. Osteocalcin immunoreactivity expression was strong in differentiating cementoblasts at the tension side. Conclusion: It can be suggested that UNC-50 is related to the differentiation of cementoblasts, and may be responsible for the molecular event in PDL cells under mechanical stress.

• Restorative Dentistry and Endodontics
• /
• v.33 no.4
• /
• pp.332-340
• /
• 2008

The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4$^{\circ}C$ for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

• The Journal of Korean Academy of Prosthodontics
• /
• v.8 no.1
• /
• pp.48-55
• /
• 1968

1958년(年) Fullmer와 $Lillie^9$에 의(依)하여 최초(最初)로 보고(報告)된 Oxytalan 섬유(纖維)는 산(酸)에 내성(耐性)이 강(强)한 섬유(纖維)로 치근막(齒根膜)에 많이 출현(出現)하고 기계적(機械的) 자극(刺戟)에 의(依)하여 배열(配列), 주행(走行) 및 수적(數的)인 변화(變化)를 가져온다. 저자(著者)는 교정력(矯正力)을 이용(利用)하여 치아(齒牙)를 이동(移動) 시킨후(後) 치근막내(齒根膜內) Oxytalan섬유(纖維)의 수(數), 주행(走行) 및 형태(形態)의 변화(變化)를 실험적(實驗的)으로 관찰(觀察)한바 있어 이를 보고(報告)하는 바이다. 본연구(本硏究)에 사용(使用)된 실험동물(實驗動物)로는 체중(體重) 60gram내외(內外)의 자성백서(雌性白鼠) 15마리를 택(澤)하였다. 각동물(各動物)은 Ether마취후(麻醉後) 교정용(矯正用) 고무줄편(片)을 상악우측(上顎右側) 제1구치(第一臼齒)와 제2구치(第二臼齒) 사이에 삽입(揷入)하여 24, 48, 72시간(時間) 간격(間隔)으로 관찰(觀察)하였다. 동물(動物)을 도살후(屠殺後) 상악골(上顎骨)을 적출(摘出)하여 10%중성(中性) 호루마린에 고정후(固定後) 3%의산(蟻酸)으로 탈회(脫灰)하였다. 파라핀 포매후(包埋後) 근원심적(近遠心的)으로 $4{\sim}6{\mu}$의 절편(切片)을 만들어 Hematoxylin-eosin 및 Aldehyde fuchsin염색(染色)을 시행(施行)하여 경험(鏡險)한 결과(結果)는 하기(下記)와 같다. 1. 치아이동후(齒牙移動後) 교원성섬유(膠原性纖維) 및 Oxytalan섬유(纖維)들의 배열(配列)에 있어 뚜렷한 차이(差異)를 보였다. 2. 치아이동후(齒牙移動後) 사주섬유(斜走纖維)는 염박측(壓迫側)에서는 치아장축(齒牙長軸)에 수직(垂直)되게, 견인측(牽引側)에서는 평행(平行)되게 주행(走行)하고 있었다. 3. 48시간군(時間群)에서 세포증식(細胞增殖)이 심(甚)하였다. 4. Oxytalan섬유(纖維)는 치아(齒牙)들에 교정력(矯正力)을 가(加)한후(後) 견인(牽引) 염박(壓迫) 양측(兩側) 공(共)히 수(數)가 증가(增加)하였다. 5. 염박측(壓迫側)에서 Oxytalan섬유(纖維)는 속(束)을 형성(形成)하며 치아장축(齒牙長軸)에 평행(平行)되게 주행(走行)하였다. 6.견인측(牽引側)에서는 하나 또는 두세개의 섬유(纖維)들이 산발적(散發的)으로 치아장축(齒牙長軸)에 평행(平行)되게 하였다. 7. Oxytalan섬유(纖維)의 수(數)는 염박측(壓迫側)에서 보다 견인측(牽引側)에서 더 변화(變化)가 많았다.

• Proceedings of the KAIS Fall Conference
• /
• 2010.05b
• /
• pp.1221-1223
• /
• 2010

본 논문에서는 한국인 아동의 우식치아로부터 S. mutans를 분리하고, acid stress하에서 분리한 S. mutans의 유전자의 발현의 변화를 분석하고자 하였다. 치아우식증의 주요한 요소로 작용하는 치태형성에 기여하는 glucan 및 fructan 합성에 관여하는 세포내 효소인 glucosyltransferase, glucosyltransferase, glucosyltransferase 및 fructosyltransferase의 발현량의 변화를 확인한 결과, lactic acid를 처리하지 않은 control의 경우보다 16배에서 3배까지 감소한 것을 확인할 수 있었다. Amino acid ABC transporter, adenylate kinase, fructokinase, 40k cell wall protein precursor에서는 모두 유전자의 발현량이 현저히 증가한 것을 볼 수 있었다. 이들 유전자는 acid stress에 관여하는 특이적 유전자로 추정된다.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2017.05a
• /
• pp.108-108
• /
• 2017

다양한 생체 재료들을 이용한 마이크로 및 나노 크기의 표면 구조 모사는 조직공학에서 세포의 성장 및 분화에 영향을 미치는 것으로 알려져 있다. 특히, 마이크로-나노 구조가 공존하는 계층적 표면 구조는 골 아세포의 증식과 분화에 탁월하여 뼈 조직 재생에 응용되어 왔다. 기존에는 화학적 처리 기법을 이용하여 마이크로 표면 구조가 제작 되었으나 미세 거칠기 및 계층적 표면 구조의 제어가 어려웠다. 현재 이러한 문제점들을 극복하기 위해 플라즈마를 이용한 애칭 기법이 주로 이용되고 있으나 높은 온도 공정 환경에 의한 재료 선택의 한계점 및 오랜 공정 시간에 의한 플라즈마 처리 효율이 감소되어 원하는 표면구조 및 거칠기를 얻을 수 없다는 단점이 있다. 본 연구에서는 이러한 문제점들을 극복하기 위해 마이크로/나노 주조 기법 이용하여 생체적합성 합성고분자 poly(${\varepsilon}$-caprolactone) (PCL) 위에 연꽃잎 구조를 모사한 후 플라즈마 애칭 기법을 이용하여 마이크로-($3.01-3.07{\mu}m$)와 나노크기 ($97{\pm}16nm$)를 동시에 갖는 계층적 구조를 제작하였다. 제작된 구조의 효능을 관찰하기 위해 조골세포를 배양한 결과 평평한 PCL 구조보다 제작된 계층적 구조가 높은 세포성장률 (>2.9배)및 세포 분화도(>2.1배)를 보였다. 이러한 결과는 새로운 표면 공학적 모델로서 손상된 뼈 및 치아조직 재생을 위한 적합한 거칠기 및 표면적인 환경을 제공해 빠른 재생 능력과 더불어 치료기간의 단축을 가져 올 수 있을 것으로 사료된다.

• Restorative Dentistry and Endodontics
• /
• v.30 no.5
• /
• pp.423-430
• /
• 2005

Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identified from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation. In this study, to better understand the biological function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry. The results were as follows : 1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended. 2. OD314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells. 3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth-ended and ruffle-ended ameloblast. These results suggest that OD314 may play important roles in the ameloblast differentiation and maturation.

• Restorative Dentistry and Endodontics
• /
• v.31 no.3
• /
• pp.192-202
• /
• 2006

The purpose of this study was to examine the viability of PDL cells in rat molars by using in vivo MTT assay, which was used to compare fast cryopreservation group by liquid nitrogen $(-196^{\circ}C)\;with\;4^{\circ}C$ cold preservation group. A total of 74 Sprague-Dawley white female rats of 4 week-old with a body weight of 100 grams were used. The maxillary left and right, first and second molars were extracted as atraumatically as possible under ketamine anesthesia. Ten teeth of each group were divided as six experimental groups depending upon the preservation. Cryopreservation groups were Group 1 (5% DMSO 6% HES in F medium) Group 2 (10% DMSO in F medium), Group 3 (5% DMSO 6% HES in $Viaspan^(R)$). Group 4 (10% DMSO in $Viaspan^(R)$) which were cryopreserved for 1 week and cold preservation groups were Group 5 (F medium) , Group 6 ($Viaspan^(R)$) at $4^{\circ}C$ for 1 week. Immediate extraction group was used as a control. After preservation and thawing, the in vivo MTT assay was processed. Two way ANOVA and Duncan's Multiple Range Test was performed at the 95 % level of confidence, Another 2 teeth of each group were treated as the same manner and frozen sections $10{\mu}m$ thick for microscopic observation. The value of optical density obtained after in vivo MTT analysis was divided by the value of eosin staining for tissue volume standardization. Group 1, 2 had significantly higher optical density than Group 3 and 4 which had the lowest OD value. Group 6 had higher OD value than in Group 5 (P<0.05). Histological findings of periodontal ligament cell, after being stained with MTT solution were consistent with the in vivo MTT assay results. In this study, the groups which were frozen with DMSO as a cryoprotectant and the groups with F medium showed the best results.