• Title/Summary/Keyword: 치상

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Effective Screening Condition for Low-Temperature Germinability of Rice (벼 저온발아성의 효율적 검정 조건)

  • Kang, Jong-Re;Lim, Sang-Jong;Kim, Soon-Chul;Ko, Mi-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.711-715
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    • 1995
  • This study was carried out to determine the desirable screeing condition for low-temperature germinability of rice seeds. The germination test was performed with fifty rice varieties from three countries, mainly including the recent Korean rice cultivars. The analyzed result of germination test under three temperature conditions(13$^{\circ}C$, 16$^{\circ}C$, $25^{\circ}C$) pointed out that 11 days after seeding at 13$^{\circ}C$ was the most efficient testing condition for low-temperature germinability in terms of mean and variation in germination rate. Highly significant correlations were observed between germination rates of 11 days after seeding at 13$^{\circ}C$ and germination coefficients, average days of germination at 13 for 20 days. Low-temperature germinability were highly positively associated with days to heading or amylose content, but negatively correlated with grain width.

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Effects of Acanthopanax senticosus herb-acupuncture on cell proliferation and nitric oxide synthase expression in dentate gyrus of alcohol-intoxicated rats (오가피(五加皮) 약침(藥針)이 알코올 중독(中毒) 흰쥐의 치상회(齒狀回)에서 신경세포생성(神經細胞生成) 및 NOS 발현(發顯)에 미치는 영향(影響))

  • Kim Jong-Chul;Lee Eun-Yong;Kim Ho-Hyun;Kim Ee-Hwa
    • Korean Journal of Acupuncture
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    • v.20 no.2
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    • pp.67-76
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    • 2003
  • 목적 : 오가피 약침이 알코올에 의해서 중독된 Sprague-Dawley(S-D)계 흰쥐의 해마 치상회에서 새로운 신경세포 생성 및 NOS발현에 미치는 영향을 조사하였다. 방법 : S-D계 흰쥐에 알코올을(2g/kg) 3일간 연속으로 투여한후, 5일간 인체의 중완혈에 상응하는 부위에 오가피 약침(30mg/kg) 치료를 시행하였다. 치료효과를 관찰하기 위해서 BrdU-면역조직화학 염색법 및 NADPH-d-조직화학염색법을 이용하였다. 결과 : 알코올 처치군에서는 BrdU-양성세포수 및 NADPH-양성세포수가 모두 정상군에 비해서 감소한 반면에 알코올 처치후 오가피 약침으로 치료한 군에서는 BrdU-양성세포수 및 NADPH-양성세포수 모두 알코올 처치군에 비해서 증가하였다. 결론 : 오가피 약침치료가 알코올에 의해서 중독된 S-D계 흰쥐 해마 치상회에서 새로운 신경세포의 생성을 증가시키는 것을 확인하였으며, 그 기전으로 산화질소가 관여할 것으로 사려된다.

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Improvement of Seed Germination in Rosa rugosa (해당화의 종자 발아 연구)

  • Lee, Ji-Yeon;Lee, Ja-Hyun;Ki, Gwang-Yeon;Kim, Seung-Tae;Han, Tae-Ho
    • Horticultural Science & Technology
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    • v.29 no.4
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    • pp.352-357
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    • 2011
  • Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at $25^{\circ}C$ room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.

Effects of Explants and Growth Regulators on Direct Somatic Embryogenesis in Liquid Culture of Scrophularia buergeriana (현삼의 액체배양에서 체세포배 형성에 대한 치상조직과 생장조절제의 영향)

  • Song, Ji-Sook;Lim, Wan-Sang;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.294-298
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    • 1998
  • The factors affecting direct somatic embryogenesis from different parts of explant in liquid culture of Scrophularia buergeriana were investigated. Direct somatic embryogenesis was dependent on the explant tissues and stem was the most efficient explant. Rapid shoot development occurred on stem after 3-week culture but roots were not developed yet. Plantlets were not formed through somatic embryogenesis after 3-week culture of petiole. Though direct somatic embryo was not observed from leaf segment culture for 3 weeks, normal plantlets were developed after 8-week culture. BA played the main role for somatic embryogenesis in liquid culture and adding of either IAA or NAA caused rather adverse effects. Culture of stem segments in MS liquid medium with BA at 0.5 mg/ l or 0.1 mg/ l was proved to be the most efficient method for producing plantlets through direct somatic embryos.

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