• Korean Journal of Environmental Biology
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• v.23 no.1
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• pp.89-92
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• 2005

The effect of cement on survival, LC/sub 50/, safe concentration (SC) and application factors of pollutant (AF) for pond smelt, Hypomesus olidus were investigated for 96 hours. Mean body length of pond smelt used in this experiment was 8.6±1.2 cm. Cement concentrations established in this experiment were 0 (control group), 10, 25, 50, 100, 250, 500, and 1,000 ppm, respectively. All fishes were dead in concentration more than 500 ppm cement within 4 hours. The LC/sub 50/ to cement toxicity was 123.03 ppm for 48h, 91.20 ppm for 72 h and 58.88 ppm for 96h. The value of SC and AF to cement toxicity for this species were 2.64∼9.14 ppm and 0.045∼0.155, respectively.

• The Korean Journal of Pesticide Science
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• v.19 no.3
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• pp.255-263
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• 2015

Promoting the organic farming, much of the plant extracts used for controlling pests and fungi have been imported from China, India and Myanmar. But, it is so worrisome that aquatic animals such as muddy loach inhabiting in paddy field and common carps in river exposed to the pests and fungi likely be harmed. This study was conducted in order to evaluate the risks of aquatic animals influenced by the three plant extracts, i.e. Sophora flavescens, Azadirachta indica and Derris elliptica. The toxicities of common carp (Cyprinus Carpio), muddy loach (Misgurnus anguillicaudatus) and PEC (Predicted environmental concentration) exposed to the three plant extracts were estimated by the typical spray volume method. Risks were determined by the toxicity value as 48-hr $LC_{50}$ (Lethal concentration, median) or NOEC (No observed effect concentration) into PEC. 48-hr $LC_{50}$ of Common carp and NOEC by Sophora flavescens extracts was 7.9 and 6.2 mg/L, 26.8 and 21.8 mg/L by Azadirachta indica extracts and 47.0 and < 24.0 mg/L by Derris elliptica extracts, respectively. 48-hr $LC_{50}$ of Muddy loach and NOEC by Sophora flavescens extracts was 16.9 and 10.0 mg/L, 35.6 and 30.0 mg/L by Azadirachta indica extracts, and 73.9 and < 40 mg/L by Derris elliptica extracts, respectively. Therefore, acute toxicities of the three plant extracts for aquatic animals were proved to be very low level. PEC of Sophora flavescens extracts in paddy, drainage and river water was 68.0~3.0, 11.33~0.50 and 3.0~0.0018 mg/L, respectively. TER of Sophora flavescens extracts in the three water was 0.2~5.6, 1.5~33.8 and 2.6~4388.9, respectively. PEC of Azadirachta indica extracts in paddy, drainage and river water was 90.9~1.2, 15.2~0.2 and 4.8~0.00075 mg/L, respectively. TER of Azadirachta indica extracts in the three water was 0.4~29.7, 2.3~178.0 and 4.5~35733.3, respectively. PEC of Derris elliptica extracts in river water was 0.0063 mg/L. TER of Derris elliptica extracts in river water was 5222~15667.

• Journal of Marine Life Science
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• v.8 no.2
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• pp.150-159
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• 2023

To determine the toxic effects of the mirror carp, Cyprinus carpio nudus on waterborne nitric acid exposure, they were exposed to waterborne nitric acid at 0, 4, 20, 100, 500, and 2,500 mg NO₃^-/L for 96 h. The semi-lethal concentration (LC₅₀) of the scented fish by 96 hours of acute exposure to nitric acid was 1,433.54 mg NO₃^-/L. The physiological changes of waterborne nitric acid exposure on mirror carp were evaluated through hematologic properties, and RBC count showed a significantly decreased (p < 0.05). The change in the ion control ability of the mirror carp according to exposure to waterborne nitric acid was evaluated through the plasma inorganic component, and calcium and magnesium were significantly decreased (p < 0.05) in the plasma inorganic component. The health and stress status of the mirror carp due to exposure to waterborne nitric acid were evaluated through plasma organic ingredients, and the plasma organic component glucose showed a significantly increase (p < 0.05). Changes in liver damage and enzyme activity due to waterborne nitric acid exposure were evaluated through plasma enzyme components, the enzymatic activities of plasma AST, ALT, and ALP were significantly decreased (p < 0.05) in high levels of waterborne nitric acid exposure. The results of the present study suggest that waterborne nitric acid exposure to C. carpio nudus can toxicly affect survival, hematologic properties, and plasma components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.12 no.2
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• pp.119-123
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• 1979

The cute toxicity test of mercury, cadmium and copper to yellowtail, Seriola quinqueradia, and to rock-bream, Oplegnathus fasciatus, were conducted by static bioassays from 18 July to 27 October, 1978. In the tests, tile least sensitive heavy metals were cadmium to rock bream and copper to yellowtail. The test species were most sensitive to mercury among all the chemicals used. The 24hr-LC 50 value for the yellowtail was 0.11mg/l for mercury, 0.82mg/l for cadmium and 1.03mg/l for copper. While the 48hr-LC 50 value for the rock bream was 1.40mg/l for mercury and 1.73mg/l for copper.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2011.04a
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• pp.441-446
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• 2011

본 연구에서는 건축물에 화재가 발생 시, 건축용 외장재로부터 발생하는 연소가스의 독성을 분석함으로써 소방관을 비롯한 건물 재실자들에게 미치는 영향에 대해서 고찰하였다. 실험 방법은 건물의 외장재로 많이 사용되는 일반스티로폼, 강화스티로폼, 우레탄폼 그리고 유리섬유 재질의 외장재를 대상으로 NES 713 방법을 이용하였다. 실험 결과를 살펴보면, 대부분의 시료들로부터 CO, $CO_2$, HCHO, $CH_2CHCN$, $NO_x$ 등이 검출되었으며, 우레판폼의 경우, 다른 시료들과 달리 HCl과 HCN이 추가적으로 검출되었다. 하지만 대부분의 시료들로부터 검출된 독성가스들은 치사농도에는 훨씬 못미치는 것으로 나타났으며, 독성지수 값들을 살펴보면, 일반스티로폼<강화스티로폼<유리섬유<우레판폼의 순으로 나타났다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.326-326
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• 1994

중금속이 양서류의 발생에 미치는 영향을 알아보기 위해 Xenopus embryo 2 할구기부터 카드뮴, 수은, 납. 구리, 아연 등을 여러 농도로 지속적으로 처리한 후 치사율과 이상 발생율을 조사하였다 그 결과 수은, 카드뮴. 구리, 납. 아연의 순으로 독성이 강함을 알 수 있다. 중금속 중 카드뮴 처리시 나타나는 현상으로는 창자, 눈, 체축. 지느러미, 십장 등의 이상과 수포 등을 들 수 있다. FETAX (frog Embryo Teratogenesis Assay : Xenopus)의 분석 결과 LC$_{100}$ 은 1.5 ppm, EC$_{100}$은 1PPm 이었고 기형 유발지수(TI)가 2. 8 인 점으로 보아 카드뮴을 Xenopus embryo에 있어서 기형유발원으로 분류할 수 있다.

• Journal of fish pathology
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• v.10 no.1
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• pp.65-72
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• 1997

In the present study, acute toxicity data were obtained for hydrogen sulfide to larvae and adults of blue crab Portunus trituberculatus, white shrimp Metapenacus monoceros and prawn Macrobrachium nipponense under continuous flow through system. The larvae 96hr-$LC_{50}$ values of hydrogen sulfide were 7.3, 9.3 and $9.0{\mu}g/\ell$ for P. trituberculatus, M. monoceros and M. nipponense respectively. The larval sensitivity of the three crustaceans studied for the hydrogen sulfide was in the order of P. trituberculatus> M. nipponense> M. monoceros. The adults 96hr-$LC_{50}$ values of hydrogen sulfide were 42.5, 37.8 and $56.6{\mu}g/\ell$ for P. trituberculatus, M. monoceros and M. ripponense, respectively. The order of toxicity of hydrogen sulfide to adults of the three crustaceans was P. tritruberculatus> M. monoceros> M. nipponense. The larval/adult ratios of hydrogen sulfide toxicity were 5.8, 4.1 and 6.3 for P. trituberculatus M. monoceros and M. nipponense respectively, and larvae were found to be more sensitive to hydrogen sulfide than adults in all cases.

• Journal of the Korean Society for Marine Environment & Energy
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• v.8 no.1
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• pp.9-16
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• 2005

A series of experiments was conducted to evaluate the effects of temperature on the survival, growth and sensitivity of the benthic amphipods, Mandibuluphoxus mai and Monocnrophium acherusicum, which have been recently developed as new sediment toxicity testing species in Korea. The biological performance for each amphipod species was determined by the survival and growth rates at different water temperatures. The influence of temperature on the sensitivity to reference toxicant, Cd, was determined by the comparison of survival rates of amphipods exposed to control and Cd-spiked seawater at different temperatures. Temperature significantly influenced on the survival, growth and Cd sensitivity of both amphipods. Tolerable ranges of temperature for the >80％ individuals of both M. mai and M. acherusicum with sediment substrates were mostly overlapped (13 to 22℃). The daily growth rates of M. mai and M. acherkisicum increased proportionally with temperature up to 20℃ and 25℃. respectively. Similarly, the sensitivities of M. mai and M. acheyusicum to Cd increased with temperature up to 20℃ and 15℃, respectively. Overall results showed that temperature is a substantially important factor potentially influencing the results of lethal and sublethal bioassays using the amphipods. Therefore, defining the adequate ranges of experimental temperature for the toxicity testing species is the pre-requisite for the development of standardized bioassay protocols.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.5
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• pp.490-499
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• 2016

In this study, the marine ecological impacts of dephosphorized slag and steel slag on the initial life cycle of the rotifer Brachionus plicatilis and benthic copepod Tigriopus japonicus (in marine trophic structure as a first consumer) exposure to slag extracts have been considered using a marine ecotoxicological assessment. In the results of a screen test on slag extracts, the pH of an undiluted solution was measured to have high alkalinity (pH 8.89-12.16), but a toxic reaction to this undiluted solution before and after aging was divided according to test species. For non-aged slag, the toxic effect ($LC_{50}$) of neonate on B. plicatilis was seen to be severe with dephosphorized slag (20.8 %) than steel slag (63.8 %) with under pH-uncontrolled conditions. The toxic values of dephosphorized and steel slag were estimated to be 35.3 % and 36.0%, respectively, for nauplius with T. japonicus. However, the toxicity of slag extracts before and after aging were different for T. japonicus than for B. plicatilis based on the characteristics of the test materials, with pH-controlled conditions. In conclusion, the results of this study suggest that slag can be relatively stable after aging and may not be likely to influence marine environments, even given repetitive extracting under pH-uncontrolled conditions. This study confirms that a marine ecotoxicological assessment method applied to mechanically activated samples can give an idea of the resistance a marine environment has against the introduction of hazardous materials due to precipitation and weathering.

• Korean Journal of Environmental Agriculture
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• v.6 no.1
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• pp.50-60
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• 1987

Sensitivity, expressed in the $96h-LC_{50}$ determined with continuous flow system, was compared for eight developmental stages of Oryzias latipes with butachlor, diazinon and fenitrothion. Significance of the laboratory determined $96h-LC_{50}$ in real field situation was evaluated for butachlor using outdoor model agrosystem. In the laboratory tests, the most sensitive stage was post-larva stage IV(7 days after hatching) with $96h-LC_{50}$ of 0.14, 1.4, and 1.6ppm for butachlor, diazinon and fenitrothion, respectively, whereas the least sensitive stage was early embryo stage(1 day after fertilization), and then the susceptibility was somewhat stable after 21days after hatching. In the model agrosystem test, the highest concentration of 0.53ppm of butachlor in water was obtained at the 2nd day after application. The stages IV and VIII showed mortality of over 50% at 96 hours after application. It is concluded that the conventional use of butachlor in the field would cause significant adverse effect on O. latipes. It is suggested that sensitivity test at the early life stages with fish is an important part of the ecotoxicological assessment of chemicals.