• Applied Biological Chemistry
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• v.37 no.6
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• pp.498-502
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• 1994

For glucosyltransferase(GTase) inhibitors, two flavan-3-ols were isolated from Theobroma cacao beam husk. They showed positive reaction with $anisaldehyde-H_2SO_4$ solution, $FeCl_3$ to be confirmed as dimeric flavan-3-ols on TLC and were identified as procyanidin B-1 [(-)-epicatechin-$(4{\beta}{\rightarrow}8)$-catechin] and procyanidin B-3 [(+)-catechin-$(4{\beta}{\rightarrow}8)$-catechin by spectroscopic analysis. Their inhibitory effect on glucosyltranaferase activity was also investigated and procyanidin B-1 showed 50% inhibition at 0.3 mM. They inhibited on the glucosyltransferasa noncompetitively and dimeric flavan-3-ol containing (-)-epicatechin had higer inhibitory activity.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.393-399
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• 1998

Enterobacter sp.B54 which shows antagonistic activity to Phytophthora capsici on potato dextrose agar was selected among 112 strains isolated from Korean soil. After Tn5 lac-induced mutants were obtained through Pl :: Tn5 lac mutagenesis, 2 mutants for loss of antibiosis and 1 mutant for increased antibiosis were screened by using in vitro fungal inhibition assay. When the 3 mutants affected in antibiosis were analyzed by southern hybridization with pRZ102 (ColEl :: Tn5) as a probe, its results suggest that Tn5 lac was randomly inserted into different chromosomal sites in these mutants.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.695-701
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• 1995

An alkaline lipase producing bacteria was isolated from soil and identified as Serratia liquefaciens AL-11. from the results of analysis of its morphological, biochemical and physiological properties. This strain showed the highest productivity of alkaline lipase when grown at pH 9.0 and 30$\circ$C for 42 hours in the medium of 1% peptone, 0.5% tryptone, 0.9% yeast extract, 1% starch, 1% tween 80, 0.05% CaCl$_{2}$ and 0.05% NaCl. The enzyme was purified by ammonium sulfate treatment, Sephadex G-100 gel filtration and DEAE-Sephadex A-50 column chromatography. The specific activity of the purified enzyme was 27 unit/mg protein and the yield of enzyme activity was 61.3%. The homogeneity of the purified enzyme was verified by polyacrylamide gel disc electrophoresis. Molecular weight of the purified enzyme was estimated about 53,000 by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. This enzyme is composed of 17 amino acids of which glycine, proline and glutamic acid were three miajor acids.

• Journal of Ginseng Research
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• v.14 no.3
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• pp.404-415
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• 1990

This study is conducted to evalute the effect of ginseng fraction component (ginseng extract solution, GES; ginseng protein, GP; ginseng saponin, GSA; ginseng residue, GR) upon hyperlipidemia and fatty liver induced by high fat administration. In doing so, the serum, liver and epididymal adpoid tissue have been examined for lipid components level and lipoprotein fraction. Feces bile acid and neutral sterol excretion have been also measured. 1'he results obtained from this study are as follows. 1. Serum, liver, epididymal lipid components of GP and GSA group were significantly lower than the controlgroup. 2. During the feeding experiment, VLDL and LDL increase while HDL decrease in all group. However the degree of VLDL and LDL increase and HDL decrease were signficantly small in GP and GSA group compared with control group. 3. In the excretion of bile acid and neutral sterol, all experiment group showed increased excretion in the comparison of control group.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.11
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• pp.1542-1551
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• 2002

The present study investigates the convective heat/mass transfer characteristics and pressure drop inside the rib-roughened cooling passage of gas turbine blades. The internal cooling passage is simulated using a square duct with h- and V-shaped rectangular ribs which have a 60。attack angle. A naphthalene sublimation technique is employed to determine the detailed local heat/mass transfer coefficients using the heat and mass transfer analogy. The ribs disturb the main flow resulting in the recirculation and secondary flows near the ribbed wail. The secondary flow patterns and the local heat transfer in the duct are changed significantly according to the rib orientation. A square duct with ∧ - and V-shaped ribs have two pairs of secondary flow due to the rib arrangement. Therefore, the average heat/mass transfer coefficients and pressure drop of ∧ - and V-shaped ribs are higher than those of the continuous ribs with 90$^{\circ}$ and 60$^{\circ}$attack angles. The ∧-shaped ribs have higher heat/mass transfer coefficients than the V-shaped ribs, and the uniformity of heat/mass transfer coefficient are increased with the discrete ribs due to the flow leakage and acceleration near the surface.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.280-287
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• 2001

The present study investigates convective heat/mass transfer and flow characteristics inside the rib-roughened cooling passage of the gas turbine blades. A square duct with rectangular ribs is used and $\wedge-$ and V-shape ribs with $60^{\circ}$ attack angle are installed on the test plate surfaces. Naphthalene sublimation technique is employed to determine the detailed local heat transfer coefficients using the heat and mass transfer analogy. The ribs disturb the main flow resulting in the recirculation and secondary flows near the ribbed wall and the vortices near the side-wall. The local heat transfer and the secondary flow in the duct are changed largely according to the rib orientation. A square duct with $\wedge$ and V-shape ribs has two pairs of secondary flow because of the rib arrangement. So, the duct has complex heat/mass transfer distribution. The average heat/mass transfer coefficient and pressure drop of $\wedge-$ and V-shape ribs are higher than those with $90^{\circ}$ and $60^{\circ}$ attack angles. The average heat/mass transfer coefficient on the $\wedge-shape$ ribs is higher than that on the V-shape ribs. Also, the uniformity of heat/mass transfer coefficient on discrete ribs is higher than that on continuous rib.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.196-201
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• 1997

Assuming that Asp225 is the substrate specificity determinant of Achromobacter pretense I (APl) which is lysine-specific serine protease, the 225th residue was substituted for other amino acids with a hope that the substrate specificity of a mutant API is altered. Furthermore, to maturate preform of mutant API autocatalytically, Lys(-1) was also replaced by Met, Asp, or Glu. However, all the mutants were not expressed, or accumulated as inactive precursor proteins. This result implicats that Asp225 plays a critical rol in restricted substrate specificity as a lysylendopeptidase but the substrate specificity of API is not determined only by the nature of residue 225.

• Applied Biological Chemistry
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• v.34 no.4
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• pp.334-338
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• 1991

Synthesis of $(D-Phe^7\;-Leu^8)$ bradykinin and bradykinin by solid phase method using a new reaction vessel was carried out. Coupling was performed by dicyclohexylcarbodiimide. After cleavage with dried HBr the peptides were purified by high pressure liquied chromatography. Their purify was assayed by paper and thin layer chromatography, melting point and amino acid analysis. $(D-Phe^7\;-Leu^8)$ bradykinin and bradykinin were incubater in vitro endopeptidase $({\alpha}-chymotrysis)$ and exopeptidase(carboxypeptidase A, leucine aminopeptidase) in order to study the degradation pattern of peptides. $(D-Phe^7\;-Leu^8)$ bradykinin and bradykinin were rapidly degradated by ${\alpha}-chymotrypsin$ and carboxypeptidase A $(D-Phe^7\;-Leu^8)$ bradykinin and bradykinin coution$(D-Phe^7\;-Leu^8)$ bradykinin and bradykinin contain imino peptide bound from proline at N-terminal and therefore they were not attacted by leucine aminopeptidase.

• Korean Journal of Environmental Biology
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• v.20 no.1
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• pp.66-72
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• 2002

The intraspecific variability, habitats, and morphological characteristics of eelgrass (Zostera marina L.) along the eastern coast of Korea were examined during June to August 1998. Morphological characteristics including shoot height, leaf length, leaf width, number of leaf veins, and shape of leaf apex were measured, and eelgrass habitats were analyzed using character correlation, principal components and cluster analyses. The morphological characteristics varied with the habitat types and water depth. Eelgrass beds distributed mostly in lagoons, ports and bays along the east coast of the Korean peninsula. The quantitative morphological features that enabled recognition of the two phonetic groups were short- narrow and long-broad leaf types. Leaf apex in particular varied with the habitat characteristics.

• Korean Journal of Food Science and Technology
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• v.27 no.1
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• pp.92-96
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• 1995

In the course of our studies, novel flavan-3-ol structure isolated from Theobroma cacao L. husk was established by the thiolysis, desulfurization and spectroscopic method. The structure was identified for cinnamtannin A-2 containing the tetrameric epicatechin and molecular weight was [1153] by FAB-MS negative ion. The inhibitory effect on the glucosyltransferase activity was investigated. Cinnamtannin A-2 showed complete inhibition at 0.03 mM and inhibited on the glucosyltransferase noncompetitively. The hydroxyl group of flavan-3-ol was supposed to be the essential element for inhibition on the glucosyltransferase.