• KSBB Journal
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• v.10 no.1
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• pp.55-62
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• 1995

The functional relationship between the initial cell concentration and the ethanol productivity was investigated in the repeated batch fermentation of Sacharomyces cerevisiae ATCC 24858. The repeated batch fermentations were performed in the range of 60 to $150g/\ell$ of initial sugar concentration and 17.5g/$\ell$ to $53.1g/\ell$ of initial cell concentration. The time of one batch fermentation was 1 or 2 hours and the batch fermentation was repeated ten times in every repeated formentaction. The functional relationship showed that the productivity increased non-linearly according to the increase of initial cell concentration regardless of initial sugar concentration. When the initial concentration of sugar was $60g/\ell$ and that of biomass was $34.5g/\ell$, the fermentation was completed within one hour and its ethanol productivity was $26.7g/\ell$.hr, the latter including the times of cell separation, pouring the new substrate into a flask and sampling. When the initial sugar concentration was $120g/\ell$ and the initial cell concentration $50.3g/\ell$, the fermentation was also finished within one hour and its productivity was $45.8g/\ell$$.$hr, The maximum ethanol productivity for eight different repealed fermentations in this work was $53g/\ell$.hr.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.641-649
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• 2020

This study was conducted to examine the effects of instruction (explicit, implicit) and stimulus presentation time (300 ms, 500 ms) in ABM training. According to the Social Avoidance and Distress Scale (SADS) and the attention bias score (ABS), 60 undergraduate students were selected. All participants' SADS scores were above 82 and their ABS score was greater than 0. The training group was explicit-300 ms training group (n=10), explicit-500 ms training group (n=10), implicit-300 ms training group (n=10) and implicit-500 ms training group (n=10). In the implicit instruction group, only information about procedure was included. In the explicit instruction group, additional information about training manipulation (the probe always presented at the location of the preceding neutral stimuli) was provided. The control group were placebo-300 ms control group(n=10) and placebo-500 ms control group(n=10). After 8 sessions of training, the effect was only found in instruction conditions. The explicit training group showed significantly decreased SADS after training. ABS changed significantly in all three conditions but the treatment effect was most effective in the explicit instruction condition. No significant effect was found in the stimulus presentation time. These results suggest that explicit instruction might promote the effect of ABM training compared to implicit instruction.

• Korean Journal of Microbiology
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• v.43 no.2
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• pp.91-99
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• 2007

For the detection of Human Immunodeficiency Virus (HIV), multiple and ultra-rapid real-time PCR methods were developed. The target DNA sequences were deduced from HIV-1 specific 495bp partial env gene (gi_1184090) and from HIV-2 specific 294 bp partial env gene (gi_1332355), and were synthesized by using PCR-based gene synthesis on the reason of safety. Ultra-rapid real-time PCR was performed by $Genspector^{TM}$ using microchip-based, $1\;{\mu}l$ of reaction volume with extremely short time in each 3 step in PCR. The detection including DNA-amplification and melting temperature analysis was completed inner 15 minutes. The HIV-1 specific 117 bp-long and HIV-2 specific 119 bp-long PCR products were successfully amplified from minimum of template,2.3 molecules of each env gene. This kind of real-time PCR was designated as ultra-rapid real-time PCR in this study and it could be applied not only an alternative detection method against HIV, but also other pathogens using PCR-based detection.

• Journal of Food Hygiene and Safety
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• v.31 no.1
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• pp.21-27
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• 2016

The objectives of this study are to produce monoclonal antibodies (MAbs) against Vibrio parahaemolyticus and to develop an immuno-selective filtration (ISF) method for the rapid and sensitive detection of V. parahaemolyticus. The characterization of the MAb produced from HKVP 4H9-9 hybridoma cell was validated by enzyme-linked immunosorbent assay (ELISA) and western blot. The produced MAb was specific to V. parahaemolyticus and showed weak cross-reaction to V. alginolyticus, V. vulnificus and Staphylococcus aureus. After optimization of the method, $5{\times}10^1cell/mL$ of V. parahaemolyticus in a pure culture could be detectable. Although weak cross-reactivity to V. vulnificus, V. alginolyticus and Staphylococcus aureus was observed, the ISF was confirmed to be highly specific to V. parahaemolyticus. Especially, the ISF showed the most sensitivity compared to the immunoassays currently reported is easier to perform and quicker than ID-ELISA.

• Journal of Aerospace System Engineering
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• v.16 no.2
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• pp.63-72
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• 2022

As interest in microsatellites increases, research has been actively conducted recently on the performance and use, as well as the orbital design and deployment techniques, for the microsatellite constellations. The purpose of this study was to investigate orbital deployment techniques using thrust and differential atmospheric drag control (DADC) for the Walker-delta constellation. When using thrust, the time and thrust required for orbital deployment vary, depending on the separation speed and direction of the satellite with respect to the launch vehicle. A control strategy to complete the orbital deployment with limited performance of the propulsion system is suggested and it was analyzed. As a result, the relationship between the deployment period and the total thrust consumption was derived. It takes a relatively longer deployment time using differential air drag rather than consuming thrusts. It was verified that the satellites can be deployed only with differential air drag at a general orbit of a microsatellite constellation. The conclusion of this study suggests that the deployment strategy in this paper can be used for the microsatellite constellation.

• Journal of Aquaculture
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• v.8 no.3
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• pp.195-207
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• 1995

In order to study the embryonic development and hatching of wild long shanny, Stichaeus grigorjewi, were caught with the gill nets in the East Sea of Korea, and stocked at indoor tanks to induce natural spawning in February 25, 1994 and February 16 to 24, 1995. They were already matured when stocked, and average body length (50.66 cm) and body weight (1,192.74 g) of 57 females and average body length (48.62 cm) and body weight (612.58g) of 43 males were recorded. Before stocking, they were inserted with identification tags(ID tags) in the dorsal muscle, and spawning was traced by the portable reader (Destron/lDl Ltd.) Forty females among 57 spawned successfully in the average of 4 days after stocking. Females spawned almost all eggs contained in the ovaries at one time in the form of an egg mass and averaging 227,200 eggs Per egg mass. The egg mass was oval in shape, translucent milky in color, 20.32cm long axis and 14.57cm short axis in size, and 803.7g in weight. Male parents guarded their egg masses and circulated water with the tail part of the body. Fertilized egg was spherical in shape, and their average diameter was 1.54 mm. Each egg had a containing single oil globule, and it's average diameter was 0.37 mm. The average water temperature was $13.2^{\circ}C$ and incubation times after fertilization were 5 hours 25 minutes up to 2-cell stage, 13 hours up to morula stage, and 66 hours 35 minutes up to embryo formation stage. Hatching rate was approximately 10 percent in 368 hours 50 minutes after fertilization, and approxionateoly 90 percent of eggs were hatched in 425 hours 30 minutes after fertilization.

• Journal of Chest Surgery
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• v.32 no.12
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• pp.1093-1099
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• 1999

배경 : 류마티스성 승모판막 폐쇄부전증과 퇴행성 승모판막 폐쇄부전증에서 승모판막 성형술의 결과와 비교하여 류마티스성 승모판막 폐쇄부전증에서도 승모팍막성형술이 적합한 치료방법이 될 수 있는지를 알아보았다. 대상 및 방법 : 95년 1월부터 98년 12월 까지 승모판막 성형술을 시행받은 184명의 환자중에서 류마티스성 승모판막 폐쇄부전증 49례(1군)의 퇴행성 승모판막 폐쇄부전증 78례(2군)를 대상으로 하였다. 평균연령은 1군이 36.3$\pm$14.6(16-74세) 2군은 52.5$\pm$13.4(14-77)세였다 총 추적 관찰기간은 1군이 72.2인년 2군이77.2인년이었다 두군에서 수술후와 수술후 6개월 1년 및 이후 1년 단위로 주기적인 심초음파를 시행하였고 이를 통계적 검정하였다. 결과 : 두 군간에 수술전 혈류역학적인 차이를 보이지 않았고 수술전 평균 승모판막 폐쇄 부전의 정도는 1군이 3.0$\pm$0.4, 2군이 3.9$\pm$0.3였으나 수술후 추적 관찰에서 각각 0.9$\pm$0.9와 0.8$\pm$0.7정도의 양호한 판막 성형술의 결과를 보였고 승모판막 면적의 변화나 승모판막에서의 평균압력차이 등 혈류역학적인 결과에도 차이를 보이고 있지 않았다 수술조기 사망과 후기 사망은 없었으며 재수술율은 1군이 인년대비 1.4% 2군이 인년대비 2.6%였고 색전발생율은 1군이 인년대비 2.8% 2군이 1.3%였다. 심내막염발생은 1군에서만 1례있었으며 상기 결과들에서 두군간에 의미있는 차이를 보이고 있지는 않았다 결론 : 향후 장기적인 추적 관찰이 필요하나 중기 성적에서 승모판막 성형술이 류마티스성 승모판막 폐쇄 부전증에서도 효과적인 치료방법임을 알수 있었다.다 출생후 폐포막의 FN의 활성은 출생후 5일 및 7일에 최고주에 달했다. 출생직후 1-2일경에 혈관의 조직내 FN의 활성이 양성을 나타내지만 3일이후 활성이감소되었다. 폐포대식세포내 FN의 활성은 출생후 증가되었다. 폐조직내 소기관지의 FN의 활성은 출생후 완만하게 상승되었다. 큰 폐포세포는 출생 1-3일에 일정량의 FN 반응이 세포질과 미세융모내에 관찰되었다. 결론 : 이상과 같은 결과로 흰쥐의 폐포의 분화과정이 계속되는 출생후 폐에서 FN의 분비는 7일이내에 성숙흰쥐의 폐포내 반응과 비슷한 반응으르 보이며 이때 폐의 실질조직은 분화가 거의 완료되었을 것으로 사료되었고 큰 폐포세포에서도 FN이 분비되는 것으로 결론지울수 있다.X>에서 $1,332.75{\mu}g/mL$으로 최 대값을 나타내었으며, 추출시간 4.24시간 및 시료에 대한 용매비 9.71 mL/g에서 가장 높게 나타났다. 추출온도가 높고, 추출시간이 증가할수록 총 polyphenol 함량이 증가하는 경향을 나타내었다. Gallic acid 함량은 $65.84^{\circ}C$에서 $30.51{\mu}g/mL$으로 최대값을 나타내 었으며, 추출시간 1.65시간 및 시료에 대한 용매비 17.17mL/g에서 가장 높은 추출율을 보였다. Gallic acid 함량에 대한 추출조건의 영향은 추출시간과 용매비에 영향을 받는 것으로 나타났으며, 설정된 범위 내에서 온도에 대한 영향은 거의 나타나지 않는 것으로 나타났다. 실험연구가 더 필요하리라 본다. 혈액학적 변화를 유도하고 환자의 연령, 혈소판 수, 대동맥 차단 시간, 체외 순환 시간, 술후 PT 및 aPTT와 같은 다인적 상황들이 술후 출혈에 영향을 미친다는 점들을 시사하고

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.7
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• pp.176-183
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• 2020

A ship has three types of diving systems (Diver Stage Diving, Wet Bell Diving and Scuba Diving) to carry out a search-and-rescue operation. To reduce the possibility of decompression sickness, any diving systems shall comply with the decompression procedure according to the decompression table corresponding to the diving depth and diving time. The decompression procedure is largely divided into two methods: underwater decompression and underwater-onboard decompression. In particular, the surface interval shall not exceed 5 minutes, which is the phase from underwater decompression to underwater-onboard decompression, in accordance with the U.S Navy Diving Manual. However, the surface interval is greater than 5 minutes as a result of using Wet Bell Diving. This paper describes the result of cause analysis and measurement with improved Wet Bell Diving. Using improved Wet Bell Diving reduced the surface interval to less than 5 minutes. The result of the research can be used for operation and improving the performance of diving systems.

• Journal of Convergence for Information Technology
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• v.10 no.2
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• pp.21-31
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• 2020

This study tested the effectiveness of commercialized smartphone apps in improving walking activities among college students. 66 participants were randomized into each of four groups that was used different behavior change strategies: monetary rewards app, goal setting and feedback app, gaming app, and self-monitoring control group. 45 participants who completed the experimentation was included in data analysis. Repeated measures ANOVA resulted in statistically significant time by groups interactions in recorded step counts, self-reported completed plans and self-reported walking activities scores. The Goal-setting and feedback group and the gaming group resulted in increase in both step counts and completed plans. The results were discussed in the framework of behaviorism.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.360-360
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• 2017

이상기상현상의 발생횟수가 지속적으로 증가함에 따라 기상 예측은 국가 재난 관리에 중요한 요소로써 부상하고 있다. 계절예측 또한 재난관리의 한 부분으로, 농업, 에너지, 수자원 그리고 공공보건 등 다양한 분야에서 잠재적 위험을 파악하는데 도움이 되는 보조 자료로 활용이 가능하다. 본 연구에서는 ICON(ICOsahedral-Nonhydrostatic) 모델을 이용하여 2015년 여름철(JJA) 강수를 예측하였다. 2015년은 장마기간을 포함한 여름철 동안 평년대비 약 절반수준(54%)에 그치는 비가 내렸으며, 태풍으로 인한 강수량도 적어 연 강수량이 평년대비 72%로 역대 최저 3위를 기록하였다. 지역별로 보면 제주도와 남해안 지방을 제외한 대부분 지방에서 강수량이 적게 나타났으며, 수도권을 중심으로는 60% 미만의 강수량을 보였다. ICON 모델은 독일 기상청(DWD)과 막스플랑크 연구소(MPI-M)에서 공동 개발하여 현업 운영중인 전 지구 모델로 비정역학 코어를 사용한다. 전 지구를 정 20면체의 삼각형으로 격자화 시켜 모든 격자의 크기가 동일하고, 극점은 1개의 꼭짓점으로 구성되어 CFL(Courant-Friderich-Lewy) 문제가 해소될 수 있다. 또한 hybrid의 병렬구조를 사용하여 전산사용 효율성을 극대화 하는 특징이 있다. 강수의 계절 예측 수행 과정은 다음과 같다. 우선, 계절예측 자료 분석 시 활용할 ICON모델의 기후값을 생산하기 위해 30년(1980년~2009년)간의 AMIP기반 규준실험을 수행한다. 다음으로, SST와 Sea ice의 평년대비 현재 변동량을 계산하고, 이 자료는 모델 적분을 수행할 때 경계 자료로서 활용하게 된다. 계절 예측은 시간 지연기법(Time-lagged method)를 이용한 앙상블예측으로 수행하며, 예측하고자 하는 계절이 시작하기 약 1개원 이전부터 1일 간격으로 전 지구 모델의 초기자료를 다르게 선택하여 총 10개의 앙상블 멤버를 구성한다. 모델의 해상도는 수평 40km, 수직 90개 층으로 구성하였으며, 적분이 완료되면 AMIP기반 실험을 통해 모의된 기후값을 토대로 예측된 계절전망 자료의 변동성을 분석한다.