• Title/Summary/Keyword: 초기 기질

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Facies Analysis of the Early Mesozoic Hajo Formation in the Chungnam Basin, Boryeong, Korea (보령지역 충남 분지 중생대 초기 하조층의 퇴적상 분석)

  • Lee, Sin-Woo;Chung, Gong-Soo
    • Journal of the Korean earth science society
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    • v.31 no.1
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    • pp.18-35
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    • 2010
  • Facies analysis of the Late Triassic Hajo Formation, the lowest stratigraphic unit in the Chungnam Basin, shows that the lower part is composed mainly of breccias or conglomerates; the middle part, conglomerates; and the upper part, conglomerates and sandstones. The formation consists of 13 facies, which include horizontally stratified clastsupported conglomerate, clast-supported massive breccia, matrix-supported massive breccia or conglomerate, matrixsupported graded conglomerate, massive pebbly sandstone, horizontally laminated sandstone, massive sandstone, graded sandstone, inversely graded sandstone, planar cross-bedded sandstone, trough cross-bedded sandstone, low angle crossbedded sandstone, and massive mudstone. These are grouped into 4 facies associations (FA). FA I consisted of clastsupported and matrix-supported massive breccias presumably deposited in the talus or upper fan delta environment. FA II consists of matrix-supported massive conglomerate and horizontally stratified clast-supported conglomerate of cobble size and it seems to have been deposited in the upper fan delta environment. FAIII consisted of matrix-supported massive conglomerate of pebble size, horizontally laminated sandstone and massive sandstone may have been deposited in the middle fan delta environment. FAIV consists of massive pebbly sandstone, horizontally laminated sandstone and massive sandstone and presumably was deposited in the lower fan delta environment. In general the Hajo Formation is interpreted to have been deposited at the talus/upper fan delta environment in early stage; it might have been deposited in the alternating environments of upper and middle fan delta in middle stage; and it seems to have been deposited in alternating environments of middle and lower fan delta in late stage.

Biodegradation of Mixture of Benzoate and m-Toluate with Pseudomonas sp. (Pseudomonas sp. 의한 Benzoate와 m-Toluate 혼합물의 생분해)

  • 정준영;김교창;조재민
    • Microbiology and Biotechnology Letters
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    • v.26 no.4
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    • pp.352-357
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    • 1998
  • Biodegradation of benzoate and m-toluate was investigated using a Pseudomonas sp. isolated in a continuous culture for 45 days with a step-wise increase of the subsrates. The optimum mixture ratio of benzoate and m-toluate was 75% and 25%, respectively. During 45-day culture, removal of benzoate and m-toluate, which was replaced 2,000 ppm on the 30th day were 94% and 79%, respectively, when COD removal rate was 80%. The enzymatic activity of catechol 1,2-dioxygenase increased and that of catechol 2,3-dioxygenase decreased as the concentration of m-toluate was increased. These results suggested that m-toluate induced enzyme activity for degradation of benzoate. The shape of isolated strain in the continuous culture was investigated with SEM and the results showed that the cell shape was more damage according to the higher concentration of aromatic hydrocarbons. Therefore, we suggested that the tolerance against aromatic hydrocarbons was related to not only enzymatic activity but also characteristic of cell membrane or cell wall.

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Hemicellulose Recovery from Lignocellulosic Material Hydrolyzed by Water (물로 가수분해된 섬유성 기질로부터 hemicellulose 회수)

  • Kim, Sung-Bae;Kim, Chang, Joon
    • KSBB Journal
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    • v.20 no.4
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    • pp.317-322
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    • 2005
  • Various recovery methods were investigated to maximize hemicellulose recovery from lignocellulosic material hydrolyzed by pure water. The pretreatment conditions of water hydrolysis were $170\~180^{\circ}C$ and 1 hour of reaction time. The percentage of hemicellulose solubilized increased as the temperature increased from 170 to $180^{\circ}C$. However, significant decomposition of sugar was observed at temperature of $180^{\circ}C$. From the results of water hydrolysis, the total amount of glucan in solid residue and liquid hydrolyzate was close to the total glucan in the original biomass. For hemicellulose, however, there was a significant difference between both contents. To prove this difference, various recovery methods were proposed. From the total sugar accountability (sugar in liquid + sugar in solid), it was confirmed that hemicellulose recovery in the hydrolyzate was increased if the product including both hydrolyzate and solid residue was physically stimulated by such as heating and ultrasound irradiation. This indicated that, in commercial scale processes that much bigger substrate sizes are used and a sufficient amount of leaching solvent can not be used after pretreatment, a significant amount of oligomers could be trapped in the solid matrix.

A Study on the Effect of Initial pH and Cultivation Temperature of Substrate on the Biomass Production and COD-reduction in the Yeast Cultivation in Sugar Beet Stillages (사탕무 알콜증류폐액을 기질로 효모균체를 생산할 때 기질의 초기 pH와 배양온도가 균체생산량과 COD감소에 미치는 영향)

  • Lee, Ki Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.13 no.4
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    • pp.100-106
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    • 2005
  • Sugar beet stillages were used as a substrate for the production of single cell protein by the thermotolerant yeasts Candida rugosa, Kluyveromyces marxianus and C. utilis. The biomass production increased in accordance with the increase of pH-value, but protein content decreased. C. rugosa showed the highest crude protein production as 3.68g/l and C. utilis 2.9g/l, Kl. marxianus 2.30g/l, respectively. The rate of COD reduction in stillage versus crude protein production of C. rugosa showed the highest value as 0.35~0.39g/l as a good strain for single cell protein production using sugar beet stillages.

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Consolidation Characteristics of Repeated Loading for Highly Organic Soils (고유기질토의 반복압밀특성)

  • 김재영;주재우;박성희
    • Journal of the Korean Geotechnical Society
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    • v.17 no.2
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    • pp.5-12
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    • 2001
  • 고유기질토의 정규압밀과 과입밀에 대한 압밀특성을 연구하기 위하여 전북지역에서 채취한 2종류의 고유기질토 IS와 YJ를 사용하여 일련의 실내 표준압밀시험과 반복압밀시험을 수행하였다. 전북지역 대표적인 고유기질토의 물리적 특성은 유기물함량이 각각 58%와 72%, 자연함수비 487%와 635%, 초기공극비 7.02와 9.72이었다. 표준압밀시험결과 K, $M_{v}$ , $C_{v}$ 는 압밀하중이 증가함에 따라서 선행하중을 경계로 일반 점토보다 크게 감소하는 특성을 보였다. 또한 2단계, 3단계 반복압밀 시험결과 K, $M_{v}$ 는 압밀하중이 증가함에 따라서 완만하게 감소하였으며 표준압밀시험결과보다는 작게 나타났다. 2단계 반복압밀의 경우 $C_{v}$ 는 정규압밀영역에서 표준압밀시험결과보다 약 10-12배정도 크게 나타났다. $C_{c}$ , $C_{a}$ 는 압밀하중이 증가함에 따라서 각각 선행하중의 약 2.5배, 3배 하중을 정점으로 크게 증감하는 고유기질토 고유의 특성을 나타냈다. 고유기질토의 $C_{c}$ , $C_{a}$ 는 일반 점토보다 압밀압력 의존성이 큰 특성을 나타냈으며 최대값도 각각 2.9-3.8, 0.3-0.4로 일반 점토보다 큰 값을 보였다. 2단계, 3단계 반복압밀시험결과 $C_{c}$ , $C_{a}$ 는 압밀하중이 증가함에 따라서 약간 증가하였다. $C_{a}$ 는 OCR이 증가함에 따라서 감소하였으며 특히 OCR이 1-2 범위에서 감소폭이 크게 나타났다. 고유길토의 제압밀계수는 반복회수 및 하중의 크기 등에 따라서 큰 영향을 받으며 또한 과압밀 크기의 대소는 고유기질토의 $C_{a}$ 에 큰 영향을 미치는 것으로 생각되어 이 분야에 대한 더 많은 연구가 필요하다.

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Valuation of Molecular Weight Distribution Charteristics of Soluble Microbial Products(SMP) Using the Batch Filtration Test (회분여과 방식을 통한 생물대사산물의 분자량 분포 특성 평가)

  • 정태영;차기철;이영무;한상국
    • Membrane Journal
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    • v.12 no.1
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    • pp.21-27
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    • 2002
  • The formation of soluble microbial products(SMP) and molecular weight distribution on loading rate were observed in batch-type culture medium, which phenol was fed as a substrate. The molecular weight destribution was obtained by using 30K, 100K dalton and $0.45{\mu}$ membrane filters. When the phenol concentrationas a substrate was 120, 230 and 440 mg/L , the specific substrate utilization rate(q) showed 0.639, 1.281 and 1.744 mgTOC/mgMLSS/day, respectively. The endogenous biomass decay rate constant($K_d$) at each substrate concentration was 0.00536, 0.0661 and 0.0749($day^1$), respectively. The $SMP_e$ product rate constant($k_{SMP}_ e$) showed 0.006, 0.0058 and 0.0057($day^1$), respectively. The initial influent substrate during the course of time degraded and produced $SMP_s$. The $SMP_s$ was converted to the $SMP_{nd}$ and endogenous phase converted to the $SMP_e$ ingredients. The molecula weight distribution on loading rate was converted to a higher MW during the course of time.

Substrate Specificities of ${\alpha}$-Galactosidase from Mortierella sp. (Mortierella sp. 유래 ${\alpha}$-Galactosidase의 기질특이성)

  • Park, Gwi-Gun
    • Microbiology and Biotechnology Letters
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    • v.39 no.3
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    • pp.245-251
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    • 2011
  • [ ${\alpha}$ ]Galactosidase was purified from a culture filtrate of Mortierella sp. by CM-sephadex C-50, and subsequent Sephadex G-100 column chromatography. The final preparation thus obtained showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be 56 kDa. $Gal^3Man^4$ ($6^3$-mono-O-${\alpha}$-D-galactopyranosyl-4-O-${\beta}$-D-mannotetraose), $Gal^{2,3}Man_5$ ($6^{2,3}$-di-O-${\alpha}$-D-galactopyranosyl-4-O-${\beta}$-D-mannopentaose), $Gal_2Man_3$ ($6^2$-mono-O-${\alpha}$-D-galactopyranosyl-4-O-${\beta}$-D-mannotriose), $Gal^2Man_6$ ($6^2$-mono-O-${\alpha}$-D-galactopyranosyl-4-O-${\beta}$-D-mannohexaose) and $Gal^2Man_5$ ($6^2$-mono-O-${\alpha}$-D-galactopyranosyl-4-O-${\beta}$-D-mannopentaose), prepared from 3 types of microbial ${\beta}$-mannnanase, were used as substrates. $Gal^3Man_4$ and $Gal^2Man_3$ had a stubbed ${\alpha}$-galactosyl residue on the $2^{nd}$ and $3^{rd}$ mannose from the reducing end of mannotetraose and mannotriose, thus ${\alpha}$-galactosidase showed a preference for stubbed ${\alpha}$-galactosyl residue. ${\alpha}$-Galactosidase hydrolyzed $Gal^3Man_4$ more rapidly than $Gal^2Man_3$. However, ${\alpha}$-galactosidase hardly acted on $Gal^{2,3}Man_5$, $Gal^2Man_6$ or $Gal^2Man_5$. The enzyme hydrolyzed melibiose to galactose and glucose, raffinose to galactose and sucrose, and also stachyose to galactose and raffinose.

Production of ${\beta}-Cyclodextrin$ from Starch by Cyclodextrin Glycosyltransferase from Alkalophilic Bacillus sp. (호알카리성 Bacillus sp. 유래의 Cyclodextrin Glycosyltransferase에 의한 ${\beta}-Cyclodextrin$의 생산)

  • Kim, Kee-Hong;Lim, Hyung-Guen;Seo, Jin-Ho
    • Korean Journal of Food Science and Technology
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    • v.25 no.6
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    • pp.608-613
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    • 1993
  • Production of cyclodextrin (CD) by cyclodextrin glycosyltransferase (CGTase) isolated from alkalophilic Bacillus sp. was carried out to determine optimal reaction conditions. The maximum initial rate of CD production from amylose was obtained at dextrose equivalent 10.5. The CD production yield showed inverse proportionality to DE values over the range from 0.5 to 37.7. Even though the deactivation constant of CGTase at $60^{\circ}C$ was higher than those at lower temperatures, the production rate and yield at $60^{\circ}C$ were still higher. These results suggest thermal stabilization of CGTase by binding with starch.

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Petrological and Mineralogical Characteristics of Matrix of Pumice in Ulleung Island (울릉도 부석 기질의 암석.광물학적 특성)

  • Im, Ji-Hyeon;Choo, Chang-Oh;Jang, Yun-Deuk
    • Journal of the Mineralogical Society of Korea
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    • v.24 no.3
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    • pp.151-164
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    • 2011
  • Mineralogical and petrological characteristics were investigated on matrix of dense gray, vesiculate gray, brown and black pumice in Ulleung Island by using XRD, FT-IR, XRF, SEM and thermal analysis. According to the analysis, most of pumice matrix are amorphous and include very small amount of sanidine and anorthoclase. Since the adsorption moistures, which commonly observed as O-H peak in FT-IR spectrum, are not identified in thermal analysis, it seems reasonable to conclude that content of the adsorption moisture has very low level. Although pumice has a large specific surface area, with long time elapsed after eruption, pumice matrix shows very low degree of hydration alteration due to the low level of water content. In SEM images, most surfaces of pumice show morphological characteristics such as various shapes of vesicle with wrinkled and thin walls resulted from ductile coalescence. Dense gray pumice formed in the initial stage includes small vesicles less than $15{\mu}m$ in size with subangular to angular shapes, free of ovoid vesicle. These characteristics are interpreted to have related to the hydrous environment derived from phreato-plinian eruption. Submicron particles observed as amorphous alumina silicate assemblages in vesicle surface are considered as particles sticked to the matrix surface through rapidly cooling process during ascent of alkali phonolitic magma. It indicates that these particles coexisted partly with crystallized alkali feldspar.

토양경작법(Landfarming)을 이용한 유류오염토양의 복원효율 평가

  • Jeon Gwon-Ho;Han Wan-Su;Jeong Hyeon-Seok;Seo Chang-Il;Park Jeong-Gu
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.219-222
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    • 2005
  • 유류로 오염된 토양에 대해 토양경작법(Landfarming)을 적용하여 복원효율을 평가하였다. 복원 초기에는 주로 휘발에 의한 오염물질의 저감이 이루어졌으며, 이 후 미생물성장에 필요한 토양뒤집기와 질소원 영양물질의 공급으로 인해 미생물수가 복원초기에 비해 약 200배 증가하면서 약 30여일 경과 후 초기 20,000ppm에서 복원목표인 TPH의 토양오염우려기준 2,000ppm의 이하인 1,910ppm으로 감소하였다. 시간이 경과할수록 탄소원 섭취기질 부족으로 완만한 오염물질 감소속도를 나타내면서 최종적으로 TPH를 측정한 결과 1,288ppm으로서 제거효율 94%이상을 나타내었다.

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