• Journal of Korean Home Economics Education Association
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• v.18 no.4 s.42
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• pp.67-84
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• 2006

This study was carried out to investigate nutrient intake, dietary self-efficacy, and physical activity self-efficacy of elementary school students by obesity index in Gyeongbuk area. The survey was conducted from June 7 to June 18 in 2005. The study was peformed by analyzing a questionnaire answered by 523 elementary school student in 5th and 6th grade. The obesity index was gained by height and weight data. Nutrient intakes was evaluated by 24hr-dietary record. Statistical data analysis were completed using the SPSS 12.0 program. The results were summarized as follows; The result of the body measurement was that 31.7% of underweight group, 45.1% of normal group, 12.8% of overweight group, 10.3% of obese group. 13.9% of underweight group experienced weight control, and 17.5% of the subject respond they will try the weight control in the future. Male students showed significantly higher exercise frequency than female students(p<0.001). Male underweight group and female obese group show the highest exercise frequency. Daily energy intake of the subject showed 80.1% in male students and 87.5% in female students revealed under the recommended dietary allowance(RDA) for Korean. The intake ratio of energy nutrients was identical in male and female of underweight group and obese group, so this showed that intake style was similar in two groups. By investigating through MAR(mean adequacy ratio), underweight group of male students and both normal and overweight group of female students were lowest, while overweight group of male students and underweight group of female students were the highest. Physical activity self-efficacy of male students was higher than female students, and physical activity self-efficacy by obesity index was higher in underweight group of male students and obese group in female.

• Parasites, Hosts and Diseases
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• v.27 no.4
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• pp.231-248
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• 1989

The growth and development of the metacercariae of F. seoulensis cultivated in vitro or on the chick chorioallantois were assessed by comparison with the optimum process of maturation in albino rats and new born chickens. The process of maturation was divided for convenience into six stages: Stage 1 ; cell multiplication, Stage 2; body shaping, Stage 3; separation of genital anlagen, Stage :1 organogeny, Stage 5; gametogony, and Stage 6: oviposition. In Hank's and Tyrode's .solutions, the metacercariae were alive up to 200 days or more at $4^{\circ}C$ without any development. The in vivo maturation process in rats or chicks was as follows: stage 1 from 6 hours; stage 2 from 24 hours; stage 3 from 48 to 72 hours; stage 4 from 3 to 4 days; stage 5 from 4 to 5 days; and stage 6 from 5 to 8 days. Despite unsuccessful infection of the metacercariae to 12 day old chicks, fully mature worms of stage 5 or 6 were recovered from new born chicks (1 to 2 days old), The metacercariae of F. seoulensis grown in vitro were up to stage 3 and no further maturation was observed. Of various media employed, the medium NCTC 109 (Gibco) or NCTC 135(Gibco) supplemented with 20% egg yolk or 20% whole egg macerate or 0.5% yeast was basically required for the earlier development of the fluke. It took 16.1 days(in average) to reach the stage 3 after cultivation. The metacercariae cultivated on the chorioallantoic membranes of 6∼13 day old chick embryo at 37∼38℃ showed their full development up to stage 5 or 6. However, the worms were in general remarkably retarded, compared with those grown in rats or chickens. In the experiments of worm transplant, although the transfer was failed from in vitro culture to in vivo of rats(Per os), the transplants from in vitro culture to the chorioallantois and from the choriollantois to in vivo of rat host were successful with or without development of the transferred worms. In the present study, it was observed that the metacercariae of F, seoulensis can be maintained in vitro media with poor development as well as fully matured in 1 to 2 day-old chicks or on the chorioallantois at a very low rate.