• Title/Summary/Keyword: 천연식물

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Antifungal Activity of Rheum undulatum on Candida albicans by the Changes in Membrane Permeability (막투과성 변화로 인한 대황의 Candida albicans에 대한 항진균 활성)

  • Lee, Heung-Shick;Kim, Younhee
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.360-367
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    • 2014
  • Candida albicans is an opportunistic and the most prevalent fungal pathogen that can cause superficial and systemic infections in immunocompromised patients. C. albicans can promote the transition from budding yeast to filamentous form, generating biofilms. Infections associated with C. albicans biofilms are frequently resistant to conventional antifungal therapy. Therefore, the development of more effective antifungal drugs related with biofilm formation is required urgently. The roots of Rheum undulatum have been used for medicinal purposes in Korea and China traditionally. The aim of present study was to evaluate the effect of R. undulatum extract upon preformed biofilms of 12 clinical C. albicans isolates and the antifungal activities. Its effect on preformed biofilms was evaluated using XTT reduction assay, and metabolic activity of all tested strains was reduced significantly ($49.4{\pm}6.0%$) at 0.098 mg/ml R. undulatum. The R. undulatum extract blocked the adhesion of C. albicans biofilms to polystyrene surfaces, and damaged the cell membrane integrity of C. albicans which was analyzed by CFDA, AM, and propidium iodide double staining. It caused cell lysis which was observed by Confocal laser scanning and phase contrast microscope after propidium iodide and neutral red staining, respectively. Membrane permeability was changed as evidenced by crystal violet uptake. The data suggest that R. undulatum inhibits biofilm formation by C. albicans, which can be associated with the damage of the cell membrane integrity, the changes in the membrane permeability and the cell lysis of C. albicans.

Flora and Life Form Spectrum of Hallasan Natural Reserve, Korea (한라산 천연보호구역의 식물상과 생활형)

  • Kim, Chan-Soo;Koh, Jung-Goon;Moon, Myong-Ok;Song, Gwan-Pil;Hyun, Hwa-Ja;Song, Kook-Man;Kim, Moon-Hong
    • Journal of Environmental Science International
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    • v.16 no.11
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    • pp.1257-1269
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    • 2007
  • This study was performed to get more knowledge on the flora and their life form spectrum in the Hallasan Natural Reserve. The vascular plants include 83 species 35 genera 12 families of pteridophytes, 5 varieties 5 species 3 families of gymnosperm, 100 taxa in 2 varieties 98 species 58 genera 8 families of monocotyledon and 362 taxa in 3 forma 10 varieties 349 species 186 genera 65 families of dicotyledon in Hallasan Natural Reserve. The total of vascular plants is 550 taxa in 3 forma 11 varieties 536 species 284 genera 88 families. Among these, 204 species in total including 19 species of pteridophytes, 2 species of conifers, 45 species of monocotyledon and 138 species of dicotyledon are found only in the Hallasan Natural Reserve. The pteridophyte-quotient is 4.52 and floristic richness is 1.94. In the analysis of life form spectrum, there are 190 species (34.5%) of hemicryptophytes and 122 species (22.2%) of geophytes so that two life forms take large portions, 312 species (56.7%) of the vegetation in Hallasan Natural Reserve. Megaphanerophytes and microphanerophytes take 14.5% including 80 species followed by 83 species (15.1%) of nanophanerophytes, 42 species (7.6%) of chamaephytes, 29 species (5.3%) of therophytes, 24 species (4.4%) of hydrophytes and 18 species (3.3%) of epiphytes. According to the life form spectrum hemicryptophytes dominated high portions, which suggests Hallasan Natural Reserve has hemicryptophytic climate and similar to middle parts in the Korean Peninsular. The differences, however, were noticed by that it has more chamaephytes and less therophytes than that of the Peninsular and partially shows the characteristics of artic or subalpine climate.

Genetic Variation of Natural Populations of Schisandra nigra in Mt. Halla (격리된 입지환경에서 형성된 한라산 흑오미자 자생군락의 유전변이)

  • 이갑연;이석우;김세현;김판기;정동준;한상섭
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.3 no.1
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    • pp.30-36
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    • 2001
  • Schisandra nigra Max. has been cultivated far a medical use as well as food. It is an endemic species which has a unique habitat at the altitude of 600-1,400 m in Cheju island. In this study, three natural populations of S. nigra were investigated by using of starch-gel electrophoresis to determine the extent and distribution of genetic diversity. Except 2 monomorphic locus (Mdh-2 and Pgi-1), 4 of the 6 isozyme locus (Idh, Mdh-2, Mnr, and Pgi-2), verified from 4 isozymes, revealed polymorphism in the three populations of S. nigra. The mean number of allele per locus was 1.7 and the percentages of polymorphism loci were 38.9% at 95% level and 50% at 99% level respectively. The observed and estimated heterozygosities were 0.141 and 0.147 respectively. Although plants which were in the face of crisis and distributed in the restricted area, have been known to the very low degree of genetic variation, S. nigra showed higher genetic variation than others. Genetic variation was mostly allocated within population and individuals than that among populations. The result of Wright's F analysis estimates of $F_{IT}$ and $F_{IT}$ showed that S. nigra population revealed Hardy-Weinberg steady state.

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상이한 삼림생태계에서의 토층분화 특성과 변화에 따른 수분 이동 특성

  • 정덕영;오종민;진연호;손요한;주영특
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1998.06a
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    • pp.69-73
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    • 1998
  • 경기도 퇴촌에 위치한 경희대학교 연습림내의 경사도와 수종을 달리하는 3개의 서로 다른 임반에서 등고선을 따라 지표면의 토층분화를 조사하였고 이에 따른 수분침투특성을 조사하였다. 3개의 임반은 낙엽송, 잣나무 및 굴참나무의 천연활엽수로 구성되었고 이에 구성 수종에 따라 지표면에 퇴적되는 O, A, B층의 깊이를 달리한다. 토층분화 길이는 3개 임반 모두 경사도가 낮은 하부사면에서 양호하게 발달하였으며 경사도가 높은 산사면이나 수간우가 많은 일부 지역에서는 유거수에 의해 유기물이나 표층토가 유실되어 바로 암반층 또는 풍화층이 나타나는 경향을 보이고 있다. 그러나 잣나무 임반의 경우 밀식 수간에 의해 유기물퇴적층과 A층의 발달이 다른 낙엽송이나 굴참나무 임반보다 토층 발달 깊이가 약 2배 이상으로 깊게 발달되었다. 그리고 조사된 임반의 하부사면의 경우 A층의 발달이 약 35cm부터 약 60cm에 이르고 있으나 토성을 구성하는 요소 중 직경 2mm 이상의 자갈에 토양입자들이 전체 토양의 40%정도를 차지하고 있다. 이러한 토층분화 특성을 달리하는 임반에서의 수분 이동특성은 퇴적된 유기물의 두께가 깊으면 깊을수록 수분침투율은 낮아지는 반면 포면 유거수량은 증가하는 경향을 보여주고 있다. 그리고 지표면, 유기물 퇴적층 아래, 지표면으로부터 30cm 아래에 설치된 Lysimeter를 이용하여 조사된 침출수의 분포는 전체를 100으로 환산시 지표면은 약 55%, 유기물퇴적층 아래는 30%, 그라고 30cm의 위치에서는 나머지 15%정도의 침출수가 포집되었다. 따라서 본 실험의 결과를 살펴보았을 때 지표 층에 존재하는 유기물이 전체 수분이동에 영향을 미치는 것으로 조사되었다.양 실험 결과, 서식지에서 조사된 결과인 잎과 줄기에서 Pb$\alpha$ 추정시에는 SeaWiFS 위성과 관련된 global algorithms 중에서 490nm와 555nm의 복합밴드를 포함하는 OC2 알고리즘(ocean color chlorophyll 2 algorithm)을 사용하는 것이 OC2 series 및 OC4 알고리즘보다 좋은 추정 값을 도출할 수 있을 것으로 기대된다.환경에서는 5일에서 7월에 주로 이 충체의 유충이 발육되고 전파되는 것으로 추측되었다.러 가지 방법들을 적극 적용하여 금후 검토해볼 필요가 있을 것이다.잡은 전혀 삭과가 형성되지 않았다. 이 결과는 종간 교잡종을 자방친으로 하고 그 자방친의 화분친을 사용할 때만 교잡이 이루어지고 있음을 나타내고 있다. 따라서 여교잡을 통한 종간잡종 품종육성 활용방안을 금후 적극 확대 검토해야 할 것이다하였다.함을 보이고 있다.X> , ZnCl$_{3}$$^{-}$같은

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Antioxidant and Alcohol Dehydrogenase Activity of Water Extracts From Abalone Containing Medicinal Plants (전복과 천연 식물류 복합물의 항산화 및 알코올대사 효소 활성)

  • Shin, Jung-Hye;Lee, Soo-Jung;Choi, Duk-Ju;Kang, Min-Jung;Sung, Nak-Ju
    • Korean journal of food and cookery science
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    • v.24 no.2
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    • pp.182-187
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    • 2008
  • This study was performed to evaluate the possible production of a functional beverage derived from abalone and with several plants (AP). Five types of AP composites were prepared having different medicinal plant compositions (AP- I : abalone, garlic, jujube, Chinese matrimony fruit, dried orange peel, licorice root, zinger, honey; AP- II: abalone, garlic, black bean, Chinese matrimony fruit, cornus fruit, licorice root, zinger, honey; AP-III : abalone, jujube, arrow root, milk vetch, dried orange peel, licorice root, zinger, honey; AP-N: abalone, black bean, arrow root, milk vetch, cornus fruit, liquorice root, zinger, honey; AP-V : abalone, garlic, Chinese matrimony fruit, milk vetch, licorice root, zinger, honey). In vitro analysis were performed to examine the antioxidant contents and alcohol dehydrogenase activities of the composites. AP- II had the highest total phenol contents ($28.55{\pm}1.56\;mg/l00\;g$), and AP- V the highest level of flavonoids ($47.61{\pm}1.58\;mg/l00\;g$). At $78.89{\pm}0.16%$, AP- V displayed the strongest electron donating ability followed by AP-II($57.99{\pm}0.21%$) and API ($37.66{\pm}0.20%$). Reducing power was also significantly higher in AP- V. The Hydroxyl radical scavenging and SOD-like activities of all composites were less than 15% and 20%, respectively. At 12.5% alcohol concentration, ADH activity ranged from $114.47{\pm}2.18{\sim}121.39{\pm}4.36%$ and ALDH activity ranged from $100.04{\pm}2.90{\sim}129.54{\pm}4.80%$; AP- I , AP- II, and AP- Vin 12.5% of alcoholic concentration. The composites of AP- I , AP- II, and AP- V, all containing garlic and Chinese matrimony fruit, were significantly stronger than AP-III and AP-N. Finally, also at 12.5% alcoholic concentration, the ALDH activity of AP- V was higher than its ADH activity.

Rhizome Growth and Flowering of White Lotus (Nelumbo nucifera) at Muan Hoesan-Lake (무안 회산지(回山池) 백련꽃의 근경생장과 개화특성)

  • Im, Myung Hee;Park, Yong Seo;Hou, Won Noung;Park, Yun Jum;Heo, Buk Gu
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.1
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    • pp.15-22
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    • 2009
  • This study was conducted to examine characteristics of rhizome growth and flowering of white lotus (Nelumbo nucifera) plant in Muan Hoesan-lake for collecting the basic data on the cultivation and the flowering which is growing in Muan Hoesan-lake of Jeonnam province in Korea. We have planted from the seeds and the enlarged rhizomes of the white lotus which were planted in the plastic containers on April 20 and May 4, 2008, and cultivated under rain shelter condition. Rhizome growth pattern and the flowering characteristics of the white lotus were examined on September 4, 2008. Main rhizomes of the white lotus propagated by the seeds and the enlarged rhizomes were branched by 10% and 15%, respectively. Numbers of nodes in the bloomed and non-bloomed rhizomes of the white lotus were over 14 and below 11, respectively. Flower was not observed in the white lotus when propagated with seeds, however, flowers came out up to 80% by the enlarged rhizomes. First flowers were bloomed from the late July and to the early August, and the number of flowers were 4.0~4.4. The first flower stalks without branching of the main rhizome were emerged in 8.8th node, however, that with branching were 8.0th node. Time to flowering from the emergence of flower stalk on the above-ground parts were required 16 days. White flowers from the lotus were 15.3 cm in height and 28.2 cm in width, their longevity was 3.3 days and the averaged flower fresh weight was 29.2 g. The results conclude that optimum number of nodes on the main rhizomes for blooming the white lotus flowers should be over 8 nodes before the rhizome enlargement.

Effect of Gamma Ray Irradiation on Seed Germination, Growth and Variant Induction in Hosta plantaginea and Farfugium japonicum (감마선 조사가 옥잠화와 털머위 종자의 발아, 생육 및 변이 유발에 미치는 영향)

  • Park, Jae Ok;Cho, Hye Sung;Park, Moon Young;Jo, Youn Seop;Kang, Si Yong;Kwack, Soo Nyeon;Heo, Buk Gu
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.2
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    • pp.128-133
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    • 2008
  • This study was conducted to select new variants with different characteristics in plant height, leaf color, and petal color of plantain lily (Hosta plantaginea) and Japanese farfugium (Farfugium japonicum). After 10-30Gy ${\gamma}-ray$ irradiation to seeds of both species, seed germination, variant induction, growth of variants, and survival ratios were estimated. Days required for seed germination were lengthened and % seed germination decreased as the dosage of ${\gamma}-ray$ increased over 20Gy. Lethal dose ($LD_{50}$) for plantain lily and Japanese farfugium were 30Gy and 25Gy, respectively. Plantain lily and Japanese farfugium treated by more than 20Gy ${\gamma}-ray$ showed dwarf characteristics in growth, and variations in leaf and petal colors. Total 166 variants in plantain lily were induced by ${\gamma}-ray$ irradiation in a $M_1$ generation, among which 12 promising variants were selected in a $M_2$ generation. Total 57 variants of Japanese farfugium were induced by ${\gamma}-ray$ irradiation in a $M_1$ generation, among which nine promising variants were selected in a $M_2$ generation.

Antioxidant Activities of Chrysanthemum frutescens, Coreopsis lanceolata, Matricaria recutica and Hieracium pilosella Flower Extracts by Extraction Solvent (추출용매에 따른 마가렛트, 큰금계국, 저먼캐모마일, 알프스민들레 꽃 추출물의 항산화 효과)

  • Woo, Jeong Hyang;Shin, So Lim;Chang, Young Deug;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.158-164
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    • 2009
  • Present studies were conducted to investigate the effects of water and 80% ethanol solvent on antioxidant activities of flower extracts of Chrysanthemum frutescens, Coreopsis lanceolata, Matricaria recutica, and Hieracium pilosella. Extraction yields of water extracts ranged 27.15~40.25% and 80% ethanol extracts 24.92~42.84%, respectively. In all species polyphenol and flavonoid contents were higher with 80% ethanol extraction, especially in C. lanceolata. Same results were obtained with scavenging effects on DPPH and ABTS radicals and ferrous ion chelating effects. Scavenging activity of flower extracts on DPPH radicals was highest in H pilosella - higher than that of synthetic antioxidant DHT. Higher scavenging activity on ABTS radicals was observed with M. recutica. Generally Compositae flower extracts exhibited higher scavenging activity on ABTS radicals than DPPH. Scavenging activity on ABTS radicals of M. recutica and P pilosella were superior to those of ascorbic acid and BHT. Ferrous ion chelating effects were much higher with H. pilosella flower extracts by 80% ethanol. Chelating effects of 4 species were much lower than those of EDTA. In conclusion, to develop natural antioxidant from above 4 Compositae species, 80% ethanol is recommended for efficient solvent to obtain maximum antioxidant isolation and activity.

Characteristics of Flowering and Fruiting in Nelumbo pentapetala 'Yellow Bird' (연 '옐로우버드'의 개화와 결실 특성)

  • Heo, Buk Gu;Park, Jae Ok;Park, Yong Seo;Im, Myung Hee;Park, Yun Jum;Kang, Jong Goo;Park, Suk Keun;Lee, Dong Han
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.81-87
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    • 2009
  • This study was conducted to examine the characteristics of flowering and fruiting in Nelumbo pentapetala 'Yellow Bird'. Lotus rhizomes were planted in pot (70 cm in length and 71 cm in width) on April 18, 2008, and grown under rain shelter condition at a private farm in Muan-gun, Jeonnam. Ninety percent of the lotus plants were bloomed as two flowers per a plant, and the others were one flower. The first flowers were bloomed in the middle or late July, and the second flowers in the early August. Flower stalks were formed between eighth and tenth internodes in the main rhizome. Nelumbo pentapetala 'Yellow Bird' had light yellow flowers, 12 cm in length and 13 cm in width. Lotus plants of 21.4% were not bloomed, resulting from the insufficient securing of internodes in the main rhizome and the blind. We, therefore, concluded that Nelumbo pentapetala 'Yellow Bird' as a ornamental plant should be grown up to 8 in the node number of main rhizome until mid-July.

Study on the Bioactive Characteristics of Morinda citrifolia as a Cosmetic Raw Material (화장품 소재로서의 노니 추출물에 관한 특성연구)

  • Kim, Seung-Heui;JANG, HYE-JIN
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.42 no.2
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    • pp.183-193
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    • 2016
  • This study attempted to investigate the possibility of the use of Morinda citrifolia (MC) as a cosmetic ingredient from its physiological activities such as antioxidant activity, cytotoxicity and anti-aging effect. MC is a tropical plant that has been used as traditional polynesian foods and medicines for over two thousand years. It has been reported that this shrub can improve antimicrobial, anti-cancer and anti-inflammatory effects and strengthen an immune system. The in vitro antioxidant activity of MC was performed to see the DPPH scavenging activity by measuring total polyphenol content and total flavonoid content. As a result, a lack of any cytotoxicity was confirmed in human dermal fibroblasts (HDF) cell. When MC extract at a concentration of over $50{\sim}100{\mu}g/mL$ was added, MMP-1 expression considerably diminished. In an in vivo test, in addition, cream containing MC extract was prepared and applied to a total of 22 women in their 30 ~ 50s in ages in the morning and in the evening for four weeks. Changes in keratin, melanin index, pore, skin color and wrinkles under the naked eyes were then comparatively measured. Keratin levels slightly increased in the control group but decreased in the experimental group. In addition, wrinkles diminished in the experimental group. This study found that MC extract controls many MMP-1 related mechanisms with great potential for use as a natural ingredient of anti-aging cosmetics.