• Korean Journal of Plant Resources
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• v.25 no.2
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• pp.176-183
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• 2012

This study was carried out to develop ferns as the natural antioxidant materials by graduating and extracting fronds of $Dryopteris$ $crassirhizoma$, $Dryopteris$ $nipponensis$, and $Polystichum$ $lepidocaulon$, which belong to Dryopteridaceae, using solvent, and analyzing the antioxidant effect of each fraction. The n-butanol fraction of $D.$ $crassirhizoma$ (550.0 $mg{\cdot}g^{-1}$), the ethyl acetate fraction of $D.$ $nipponensis$ (374.8 $mg{\cdot}g^{-1}$), and the n-butanol fraction of $P.$ $lepidocaulon$ (781.8 $mg{\cdot}g^{-1}$) showed relatively higher total contents of polyphenol. The chloroform fraction of $D.$ $crassirhizoma$ (72.9 $mg{\cdot}g^{-1}$), and the n-hexane fraction of $D.$ $nipponensis$ (72.9 $mg{\cdot}g^{-1}$) and $P.$ $lepidocaulon$ (154.5 $mg{\cdot}g^{-1}$) contained relatively higher total contents of flavonoids. DPPH radical scavenging activity was most excellent in the n-butanol fraction of $D.$ $crassirhizoma$ ($RC_{50}=0.02mg{\cdot}mL^{-1}$) and $P.$ $lepidocaulon$ ($RC_{50}=0.04mg{\cdot}mL^{-1}$), and the water fractions of $D.$ $nipponensis$ ($RC_{50}=0.01mg{\cdot}mL^{-1}$). ABTS radical scavenging activity was potent in the n-hexane and n-butanol fractions of $D.$ $crassirhizoma$ (each $RC_{50}=0.02mg{\cdot}mL^{-1}$), the ethyl acetate fraction of $D.$ $nipponensis$ ($RC_{50}=0.03mg{\cdot}mL^{-1}$), and the n-butanol fraction of $P.$ $lepidocaulon$ ($RC_{50}=0.06mg{\cdot}mL^{-1}$). There was the large amount of total polyphenol content in the n-butanol fraction of $D.$ $crassirhizoma$ and $P.$ $lepidocaulon$, and their radical scavenging activities were potent. Therefore, it was thought that biologically active substances of each fraction layer are required to be analyzed and used.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.16-16
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• 2010

본 연구는 13과 37종 양치식물의 엽상체(지상부)와 근경의 메탄올 추출물을 대상으로 식품의 오염 및 변질의 원인이 되는 Bacillus subtils, 식중독의 원인이 되는 Escherichia coli, 식중독 및 선회병(listeriosis)의 원인이 되는 Listeria monocytogenes, 피부 여드름의 원인이 되는 Propionibacterium acnes에 대한 항균활성을 탐색하고 항균력이 우수한 천연소재를 선발하기 위하여 시행하였다. $5mg{\cdot}mL^{-1}$의 농도의 추출물을 대상으로 각 미생물에 대하여 50% 이상의 항균활성을 나타낸 양치식물 추출물을 선발하였으며, 선발된 추출물을 2mg/disc의 농도로 조절하여 agar diffusion법으로 항균력을 측정하여 15mm 이상의 억제환을 나타내는 추출물을 선발하였다. 상기의 방법으로 선발된 추출물을 $0.125{\sim}2.000mg{\cdot}mL^{-1}$의 농도로 조절하여 72시간 동안 각 미생육의 생육에 미치는 영향을 분석하여 항균활성이 우수한 양치식물의 추출물을 선발하였다. 연구의 결과, B. subtils의 생육억제에는 야산고비의 성엽과 쇠고비의 근경 추출물, E. coli의 생육 억제에는 설설고사리의 성엽과 나도히초미 및 더부살이고사리의 근경 추출물, L. monocytogenes의 생육억제에는 관중의 성엽과 공작고사리, 꿩고비 및 쇠고비의 추출물이 효과적이었다. 한편 대부분의 양치식물 추출물은 P. acnes의 생육억제 효과가 미비하였으나, 관중과 참지네고사리는 성엽과 근경 모두에서 P. acnes의 생육억제 효과가 매우 우수하였으며, 별도의 제균처리를 하지 않은 관중과 참지네고사리의 메탄올 조추출물의 $MIC_{50}$은 성엽의 추출물에서 66.8과 $80.4{\mu}g{\cdot}mL^{-1}$, 근경의 추출물에서는 8.8과 $54.2{\mu}g{\cdot}mL^{-1}$로 나타났다.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.459-476
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• 2008

Taxonomic characteristics of spores of 17 species belonging to 6 genera of the Korean Thelypteridaceae are examined. Description of each species and key to the species are provided. Spores of Korean Thelypteridaceae are monad and monolete with bilateral symmetry. Perispore, the sporoderm of spore, was found in all the species examined. The perispores and exospores of Thelypteris japonica var. japonica, T. japonica var. glabrata and Pseudocyclosorus subochthodes are much similar to each other, but the rest of taxa examined shows diverse patterns of the surface ornamentation of perispores and exopores enough to distinguish genera and sometimes to distinguish species in some genera. Previous study reported that perispore is not easily distinguished from exospore in Cyclosorus and Leptogramma. However, three species of Cyclosorus examined here show different pattern having scabrate or verrucate exospores and cristate or echinate perispores. Leptogramma pozoi ssp. mollisima also have echinate perispore and fossulate exospore. Macrothelypteris oligophlebia var. elegans and M. viridifrons have scabrate exospore which is contrast to previous report of reticulate exospore. Thelypteris glanduligera, T. angustifrons and T. laxa show same patterns of perispore and exospore with reticulate perispore and fossulate exospore and hence cannot be distinguished by spores only.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.349.2-349.2
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• 2007

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.359.2-359.2
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• 2007

• Journal of Bio-Environment Control
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• v.20 no.2
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• pp.109-115
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• 2011

This study was carried out to investigate the plant growth of various ferns affected by the shading and fertilizer application and to recommend the appropriate levels of light intensity. Evergreen ferns like Dryopteris nipponensis Koidz., Cyrtomium falcatum (L. f.) Presl, Onychium japonicum (Thunb.) Kunze and deciduous ferns like Athyrium niponicum (Mett.) Hence, Thelypteris decursive-pinnata Ching, Athyrium brevifrons Kodama ex Nakai were used in this experiment. Evergreen ferns showed the best growth in the shading level of 30~60%. The plant growth and ornamental value (leaf color) of these plants were improved according to the fertilizer application in appropriate shading levels. The survival rate remained constantly in all treatments. Deciduous ferns showed the best growth in different shading levels from 0 to 60%. Some treatments showed improved growth according to fertilizer application, whereas survival rate was in the other treatments. There were differences in plant growth of evergreen and deciduous ferns according to fertilizer application, and additional researches must be executed to explain these reponses.

• Korean Journal of Environment and Ecology
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• v.26 no.6
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• pp.839-867
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• 2012

Vascular plant surveys in Iss. Youngjongdo and Yongyudo, Incheon were conducted 6 times from May to October 2009 and July 2011. The vascular plants inventoried during the surveys were 644 taxa in total; 11 formas, 73 varieties, 4 subspecies, 556 species, 352 genera and 98 families. Among them, 250 taxa were newly found from the survey areas, including Cnidium japonicum Miq., Argusia sibirica (L.) Dandy, Breea setosa (Willd.) Kitam., Dryopteris erythrosora (D. C. Eaton) Kuntze and Dryopteris nipponensis Koidzumi. In the case of dormancy form, hemicryptophytes were most abundant by 37.1%. Six taxa of Korean endemic plants and nine Korean rare plants were also newly recognized along with 45 taxa of notable plants of regional flora announced by the Ministry of Environment. The naturalized plants were 95 taxa, and the Urbanization Index of the survey areas was 29.6%.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.480-489
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• 2017

We investigated a suitable method for in vitro germination of spores, propagation of prothalli, and the formation of sporophytes in the fern Dryopteris nipponensis Koidz. Spore germination rate was relatively high regardless of culture medium. Prothallus development was faster in Knop medium than in Murashige and Skoog (MS) media. Prothalli used in all experiments were obtained from germinated spores, and were cultivated in different concentrations of media components. The active formation of sexual organs such as antheridium made 1MS medium suitable for prothallus propagation, although there was a lower propagation ratio compared to Knop medium. Growth and morphogenesis of prothalli were most effective on 1MS medium containing 2% sucrose, and 60 mM of total nitrogen source with 20:40 mM ratio of $NH_4{^+}:NO_3{^-}$. To select a suitable soil composition for sporophyte formation, ground prothalli were cultivated on single and mixed soils using bed soil, peat moss, perlite, and decomposed granite for 14 weeks. Bed soil promoted sporophyte formation and growth regardless of single or mixed use. In particular, a mixture of bed soil and decomposed granite in a 2:1 ratio (v:v) led to accelerated sporophyte formation ($0.83/cm^2$).