• Journal of Aquaculture
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• v.16 no.4
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• pp.257-261
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• 2003

The effects of different stocking densities on the growth of juvenile abalone, Haliotis discus hannai and water quality in the recirculating system with rotating biological contactor, were assessed. The trials were conducted using total weight 32 g juveniles abalone for 60 days at stocking densities of 5, 10, 15 kg/$m^2$. The animals were fed sufficient amounts of the pellet diet of 30.4% protein during the experimental period. Daily feeding rate (DFR) and survival rate (SR) of 5 kg/$m^2$ showed 0.76% and 85.5% those were not significantly differed 10 kg/$m^2$ showing 0.75% and 96.0% (P>0.05). DFR and SR of 15 kg/$m^2$ showing 0.38% and 31.2% were significantly lower than 5 and 10 kg/m2 (P<0.05). Daily growth rate (DGR) and feed efficiency (FE) showed the highest as 0.19% and 24.2% in 5 kg/$m^2$ the lowest as 0.05% and 14.3% in 15 kg/$m^2$ (P<0.05). According to, these results was an inverse relationship between growth and stocking density. The optimum density of juvenile abalone from this experiment is 5 to 10 kg/$m^2$ in the recirculating system.

• Journal of Marine Life Science
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• v.2 no.2
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• pp.39-48
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• 2017

This study was carried out to investigate the stress response of pacific abalone exposed to various water temperatures (4, 6, 8, and 10℃) and salinities (26, 30, and 34 psu) for 7 days, with the aim of finding optimum conditions for long-distance ocean transport of pacific abalone. At the end of the experiments, the survival rate was ranged from 98.7~100% at 8 and 10℃ but dropped to 25~55% at 4℃ in all salinity levels. The levels of SOD and glutathione in hemolymph were significantly higher at 4 and 6℃ than the control in all salinity groups, indicating that these temperatures induce severe stress in pacific abalone. It was found that THC was lowest at 6℃ in the 26 psu groups. The study showed that the hemocyte of pacific abalone populations mostly consisted of blast-like cells and hyalinocytes with the ratio of hyalinocytes being significantly lower at 4 and 6℃ than the other temperatures in the 26 psu groups. Percentages of apoptotic cells and necrotic cells were higher in the 26 psu group and 4 and 6℃ temperature groups. These results explicit that pacific abalone was exposed to greater stress at 26 psu and at 4 and 6℃ but experienced no significant higher stress at 30 and 34 psu and 8 and 10℃. It was therefore concluded that the optimum temperature and salinity for the long distance transport of pacific abalone range from 8~10℃ and 30~34 psu, respectively.

• Journal of Marine Life Science
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• v.1 no.1
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• pp.50-55
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• 2016

Glucose was continuously increased as time was elapsed under the temperature at 6℃ and 4℃. NH₃ was significantly increased at 4℃. AST was increased as temperature and salinity were decreased which indicated that it was affected by temperature, salinity and interaction of temperature×salinity. ALT was significantly increased as temperature was decreased. Free amino acid contents, excluding aspartic acid, threonine, serine and glycine were increased as temperature was decreased. This decreasing tendency was also observed when salinity was decreased. Taurine, aspartic acid, glutamic acid and arginine were significantly increased as salinity was decreased, while glycine, alanine, methionine, tyrosine, and histidine were decreased as salinity was decreased. The result of this study suggested that transport condition to minimize the stress is considered in the range of 30~34 psu, 8~10℃.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.81-87
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• 2003

The digestive system of the abalone, Haliotis discus hannai consists of radula sac, esophagus, crop, stomach, intestine (anterior, mid and posterior intestine) and hepatopancreas. The epithelial layer was composed of ciliated columnar cells, mucous cells and granular cells. And epithelium thickness of the crop was thicker (90.80 ${\mu}$m) than those of other regions. Mucous cells of PAS positive in the esophagus were more advanced than those of other regions. The contents of mucous cell were neutral and acid mucosubstance in the esophagus and the anterior, mid and posterior intestine. And it seem to be lipid in the crop and stomach.

• Journal of Aquaculture
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• v.11 no.2
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• pp.159-166
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• 1998

This study was carrie out to develop practical feed formulation for juvenile abalone (Haliotis discus hannai). Three replicate groups of abalone averaging 120mg were fed one of 7 isoproteic (34%) and isolipidic (5.3%) dlets containing various practical ingredients such as casein, fish meal, soybean meal, cottonseed meal, Undaria powder, wheat flour, spirulina, starch, and/or wheat germ meal for 4 months. The prices (5,000~800 won/kg diet) of these experimental diets were adjusted by adding different levels and ratios of practival ingredients. In addition, these experimental diets were compared with commercial feed or natural food (Undaria). Weight gain, soft body weight and shell growth of abalone fed experimental diets with low prices (2,500~800 won/kg diet) and commercial diet were significantly higher than those of abalone fed experimental diet with high price (5,000 won/kg diet)and natural food (P<0.05). The results indicate that our economic formulation (about 1,000 won/kg diet) can be useful for juvenile abalone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.391-394
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• 1999

A feeding experiment was conducted using juvenile abalone (Haliotis discus hannai) to investigate the optimum level of supplemental vitamin and/or mineral premixes in a formulated diet for practical feed formulation. Three replicate groups of abalone averaging 100 mg were fed the diets containing different levels of vitamin premix ($0\%,\;0.5\%,\;1.0\%$ and $2\%$) and /or mineral premix($0\%,\;2\%,\;4\%$ and $6\%$) for 4 months. Survival rates of abalone showed no distinctive trend by the levels of dietary vitamin premixes. Weight gain, shell growth and soft body weight of abalone were not significantly affected by the different dietary vitamin and/or mineral premixes (P>0,05). Moisture, protein and lipid contents of soft body were influenced by experimental diets (P<0.05) but these differences also had no definite trends by levels of vitamin and/or mineral premixes. These data indicate that supplemental vitamin or mineral premix is not needed in this formulated diet, therefore price of diet for juvenile abalone could be reduced.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.3
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• pp.290-294
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• 1999

An 18-week growth trial was conducted in flow-through aquarium system to investigate the effects of several additives in formulated diets on the growth and body composition of juvenile abalone (Haliotis discus hannai). Four replicate groups of abalone averaging 150 mg were fed one of four isonitrogenous ($33.8\%$) and isolipidic ($5.8\%$) diets containing $5\%$ squid meal, $0.5\%$ attractant, $1\%$ herb or $0.5\%$ lecithin. In addition, these formulated diets were compared to commercial diet. Survival rate and shell growth of abalone were not significantly affected by the different dietary additives and commercial diet (P>0.1). Body weight gain of abalone fed the diet containing herb was higher than those of abalone fed the diets containing squid meal or lecithin and commercial diet (P<0.1). Moisture, protein, lipid and ash contents of soft body were not influenced by dietary additives and commercial diet (P>0.1). The results suggest that herb as additive in this formulated diet can improve weight gain of abalone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.869-874
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• 1998

A feeding trial was carried out to investigate the optimum density of juvenile abalone (Haliotis discus hannai) fed the formulated diet or macroalgae ( Undaria). Abalone with initial average shell length of 8.41 mm were stocked into three replicate tanks at four different densities of 1430, 2860, 4290, and 5720 individual/$m^2$ for 15 weeks. The formulated diet produced higher body weight and shell growth, soft body weight and survival rate than Undaria in abalone. Abalone fed the formulated diet have significantly (P<0,05) higher lipid and ash, and lower moisture and protein in soft body. Higher density resulted in lower growth and survival (P<0.05). Whereas shell growth, survival rate and weight gain were not significantly (P>0.05) different within the abalone stocked at stocking densities of 1430 and 2860 individual/$m^2$ in each diet group. Body composition of abalone was not affected by increasing density. These study indicate that the number of abalone (shell length ranges of between 8 mm and 17 mm) below 2860 individual/$m^2$ were predicted to effective density in the indoor tank culture system.

• Journal of fish pathology
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• v.11 no.1
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• pp.35-41
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• 1998

We investigated the optimal concentration of lidocaine and MS-222 (tricaine methanesulfonate) for the exfoliation and recovery of abalone, Haliotis discus hannai in different shell lengths, for the purpose of preventing the damage of shell and muscle. However, most anaesthetics applied at present have a strong toxic effect on abalone. MS-222 is the only anaesthetic which is approved for use in food fish by FDA, and lidocaine belongs to a group of anaesthetic which are used in human medicine. These chemicals were evaluated as anaesthetic for different shell size of abalone. The response varied for different shell size groups (shell length 1, 2 and 3 cm). In this study, we suggested the result that the exfoliation and recovery time by lidocaine and MS-222 in shell length 1 cm group were more shorter than in 3 cm group. In shell length 1 cm group, the optimal concentrations of lidocaine and MS-222 for anaesthetic were 200 ppm and 100 ppm, respectively. Lidocaine and MS-222 are preferable to other conventional abalone anaesthetics since these are cheap, safe and convenient to use.

• Journal of Aquaculture
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• v.10 no.4
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• pp.417-424
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• 1997

This study was carried out to investigate the effects of experimental formulated diets, commercial diets and natural diet(dried Undaria) on growth and body composition of Haliotis discus hannai. Experimental diets was formulated to provide 34.1% protein and 7.5% lipid, and prepared by different $CaCl_2$ concentration (5, 10 and 15%) and dipping time (10, 20 and 60 second) in solution. The juvenile abalone average weighing 0.3g were distributed in a flow-through aquarium system using a cmpletely randomized desing with 13 diets and 3 replictes per diet (50 abalone/tank), and fed diets for 17 weeks. The abalone fed the time nine experimental formulated diets and two commercial diets showed better growth performance than that of natural diets. No significant differences were found among abalone fed the experimental diets and commercial diets in survival rate, weight gain, soft body weight and soft body composition (P>0.05).