• Clinical and Experimental Reproductive Medicine
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• v.35 no.2
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• pp.99-110
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• 2008

Objectives: Preimplantation genetic diagnosis (PGD) has become an assisted reproductive technique for couples carrying genetic conditions that may affect their offspring. Osteogenesis imperfecta (OI) is an autosomal dominant disorder of connective tissue characterized by bone fragility and low bone mass. At least 95% of cases are caused by dominant mutations in the COL1A1 or COL1A2. In this study, we report on our experience clinical outcomes with 5 PGD cycles for OI in two couples. Methods: Before clinical PGD, we assessed the amplification rate and allele drop-out (ADO) rate of alkaline lysis and nested PCR protocol using heterozygous patient's single lymphocytes in the pre-clinical diagnostic tests for OI. We performed 5 cycles of PGD for OI by nested PCR for the causative mutation loci, COL1A1 c.2452G>A and c.3226G>A, in case 1 and case 2, respectively. The PCR products were analyzed by agarose gel electrophoresis, restriction fragment length polymorphism (RFLP) analysis with HaeIII restriction enzyme in the case 1 and direct DNA sequencing. Results: We confirmed the causative mutation loci, COL1A1 c.2452G>A in case 1 and c.3226G>A in case 2. In the pre-clinical tests, the amplification rate was 94.2% and ADO rate was 22.5% in case 1, while 98.1% and 1.9% in case 2, respectively. In case 1, a total of 34 embryos were analyzed and 31 embryos (91.2%) were successfully diagnosed in 3 PGD cycles. Eight out of 19 embryos diagnosed as unaffected embryos were transferred in all 3 cycles, and in the third cycle, pregnancy was achieved and a healthy baby was delivered without any complications in July, 2005. In case 2, all 19 embryos (100.0%) were successfully diagnosed and 4 out of 11 unaffected embryos were transferred in 2 cycles. Pregnancy was achieved in the second cycle and the healthy baby was delivered in March, 2008. The causative locus was confirmed as a normal by amniocentesis and postnatal diagnosis. Conclusions: To our knowledge, these two cases are the first successful PGD for OI in Korea. Our experience provides a further demonstration that PGD is a reliable and effective clinical techniques and a useful option for many couples with a high risk of transmitting a genetic disease.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.251-251
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• 2004

Plasminogen activators (PA)는 다수의 세포 형태에서 분비되는 것으로 알려진 serine protease이다. PA는 섬유소 용해, 배란, 유선 퇴화, 착상 및 수정 등 다양한 생리적인 과정에 관여한다. 본 연구는 난포액이 돼지 난자의 체외성숙에 미치는 영향을 검토하기 위하여, 다양한 조건하에서의 돼지 난자의 성숙과 난구세포-난자 복합체(Cumulus-Oocyte complexes: COCs) 또는 conditioned medium 내의 PA 활성을 검토하였다. 직경 2∼6m 난포로부터 COCs를 회수하여 일부는 난구세포를 제거하였다. (중략)

• Proceedings of the SAREK Conference
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• 2003.07a
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• pp.46-46
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• 2003

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.804-809
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• 2009

The present study measured frost pattern on a cylinder to propose empirical correlation equations for the local and average frost thickness, frost density and frost mass. The key parameters were diameter of the cylinders (7mm, 20mm), cooling surface temperature of the circular tube, absolute humidity of air, air temperature and air velocity. A 50% ethylene glycol aqueous solution was applied as a coolant. The frost thicknesses at both front and rear were larger than those at the other parts, while they were increased as diameter of the cylinder was increased. The local frost thicknesses at high air velocity were more uniform than those at low air velocity. The values predicted by Kim et al. under the freezer condition showed larger by the maximum of $30{\sim}50%$ than the measured data under heat pump condition. The empirical correlations for the local and average frost thickness and frost mass were proposed. The correlation equations for the frost thickness and frost mass under the heat pump condition in the present study might predict more accurate than the other correlation equations. The proposed correlations might be applied for the freezer condition within the maximum 15% deviation from the previous correlations under freezer condition.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.7 no.1
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• pp.161-170
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• 1995

This study is concerned with the numerical analysis of performance on fin-tube heat exchanger under frosting condition. In this work, tube-by-tube method using LMED is employed. The present results are compared with O'Neal's experimental and numerical results. A standard evaporator model with 2rows-2columns is selected to investigate the effects of the various parameters such as fin pitch, air flow velocity, and humidity. The results show that frost thickness and the amount of frost per unit area decrease as fin-pitch becomes narrower. In the meantime, frost thickness and accumulation rate increase with higher inlet air humidity. It is shown that heat transfer rate increases during 30minutes and then it decreases. Heat transfer rate and the amount of frost increase with air velocity, however frost thickness does not increase over a certain velocity.

• Journal of Energy Engineering
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• v.14 no.4 s.44
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• pp.213-218
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• 2005

In order to understand the heat and mass transfer characteristics of humid airflow in frosting conditions, a flat plate of aluminum with cooling modules located in the central part of the plate was used. A microscope system (resolution of 0.05 mm) was used for the measurement of local thickness of frost at seven points along the plate in the flow direction. For the total mass of frost at each test operation, an electronic balance (resolution of 1 mg) was used. The local frost thickness distributions far various test conditions were presented along with the frost mass data measured at the given operating times. The effect of humidity and velocity of humid air on frosting were analyzed.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.31 no.3
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• pp.53-58
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• 2002

대부분의 가정용 냉난방겸용 공기조화기(히트펌프)는 실외온도가 $5^{\circ}C$ 이하이고, 습도가 높은 저온고습조건에서 난방운전을 하면 열교환기 표변에 서리가 발생하고, 이로 인해 시스템의 성능이 떨어지게 된다. 이를 방지하기 위하여 어느 일정 조건이나 시간이 되면 히트펌프는 제상운전을 하게 되는데, 보통의 가정용 히트펌프의 경우 난방운전도중에 사이클을 냉방운전으로 전환하여 실외 열교환기의 온도를 높여 줌으로써 제상을 하는 방식 (Cycle reversing method)을 사용하고 있다. 이 방식은 부가적인 제상회로플 구성하 거나 히터를 첨가하는 등의 재료비 증가 없이 제상을 수행한다는 장점이 있으나, 시스템의 에너지 효율 측면에서 불리할 뿐만 아니라, 소비자의 입장에서 보면 제상운전이 진행되는동안에 따뜻한 바람이 공급되지 않아 쾌적한 공조를 제공받지 못한다는 단점이 있다. 본 자료에서는 먼저 제상방석에 관하여 그 종류와 특정을 알아보고, 최근에 진행되고 있는 착상 지연기술 중 어큐물레이터 내에 히터를 설치하는 방식(Viung C. Mei, et al., 2002, ASHRAE TRANSSACTIONS, V 108, Pt. 1)에 대하여 소개하고자 한다.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.347-353
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• 1997

This study was to test whether in vitro/in vivo survival of vitrified mouse blastocysts was influenced by culture conditions and ET method. Mouse blastocysts were obtained from in vitro fertilization and cultured for 4 days in M16 medium, and they were vitrified in EFS40 which contained 40% ethlyene glycol, 18% Ficoll and 0.5 mol sucrose in PBS. In experiment I, in vitro and in vivo survival rate of these embryos were evaluated in different culture condition after thawing. When thawed embryos were cultured in M16 medium as a control, m-CR1 medium contained 20 amino acids (2% BME amino acis and 1% MEM non-essential amino acids solution) and 4 mg/ml BSA and cumulus monolayer cell co-cultured condition in mCR1 medium (10% FBS), their in vitro survival at 24 hr after thawing was not affected by culture condition (75.6, 83.1, 82.4%). However, in vivo survival rates of implantation in m-CR1 medium (80.4%) were significantly higher than those of M16 medium (51.2%), co-culture (57.1%) condition, although there was no difference in live fetuses rates on day 15 gestation (39.0, 49.0, 38.1%). In experiment II, the in vivo development potential of embryos by ET methods was examined. When blastocysts were transferred to the day 2, 3 pseudopregnant recipient without culture soon after thawing, no pregnant recipient was obtained on the day 2 pseudopregnancy, and 50% of pregnancy rates and 15.4% of live fetus rates were obtained on the day 3 pseudopregnant recipients. These results were significantly lower than those of transferred group (day 3 pseudopregnant recipients) after culture for 16 hr post thawing (73.5, 57.1%) (p<0.05). In experiment III, to elevate usability of delayed embryos in vitro/in vivo survival of vitrified embryos (day 4 early, day 5 early and expanding blastocyst) were examined. in vivo survival rates (live fetus, total implantation) were higher in day 4 early blastocysts (33.3, 66.7%) than in day 5 expanding blastocysts (29.0, 38.7%), although the highest in vitro survival rates were obtained in the day 5 expanding brastocysts (78.3%). Therefore, these results suggest that the in vitro/in vivo survival rates of vitrified embryos could be improve by the culture condition and ET method and that the in vivo development rates of delayed embryos were decreased with longer culture duration in vitro. It means that more effective cryopreservation was obtained in day 4 early blastocysts than in day 5 expanding blastocysts.

• Development and Reproduction
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• v.4 no.1
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• pp.29-35
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• 2000

A phosphatidylinositol 3-kinase (PI3K) is a upstream component of insulin signaling by which protein synthesis can be stimulated in many systems. To elucidate involvement of PI3K and its downstream mammalian target of rapamycin (mTOR) in the insulin signaling in pleimplantation mouse embryos, 8-cell embryos were cultured to blastocysts in the presence or absence of insulin and／or inhibitor drugs. The number of blastomeres per blastocyst, protein synthesis, and protein phosphorylation were examined. There was significant difference in embryonic development to blastocyst stage and hatching was potentiated by the insulin supplementation. The increase in the mean celt numbers per blastocyst was apparent in the insulin culture. Wortmannin, a PI3K inhibitor and rapamycin, an inhibitor of mTOR abolished the stimulatory effect of insulin on morphological development mitosis and protein synthesis. In autoradiography, phosphoproteins pp22 and pp30 which undergo phosphorylation in response to insulin were identified. Taken together, it can be suggested that PI3K and mTOR engaged in insulin signaling in the mouse embryo 8-cell onward and mediate embryotropic offset of insulin.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.25 no.9
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• pp.499-505
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• 2013

R744 has been appropriated for substitute refrigerant, because of its high stability, and environment-friendly nature as a natural refrigerant. To analyze the cooling performance of a refrigeration system in a refrigerator truck using R744 according to the blocking ratio, an analytical model of the refrigeration system was developed under frost conditions, using EES. The performance of the refrigeration system was predicted with the indoor and outdoor air temperature, outdoor air velocity, and compressor speed. As a result, the system performance decreased, with the increase of frost growth. When the blocking ratio was 40.4% in the basic condition, the refrigeration capacity was decreased by 27.1%, compared to the non-frost condition.