• Title/Summary/Keyword: 질소고정

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Effects of herbicide butachlor on Rhodospirillum rubrum KS-301 (Rhodospirillum rubrum KS-301에 미치는 제초제 butachlor의 영향)

  • 이경미;이현순
    • Korean Journal of Microbiology
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    • v.27 no.3
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    • pp.285-290
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    • 1989
  • The biololgical effect of the preemergence rice field herbicide, butachlor(commercial name, Machete) on purple nonsulfur photosynthetic bacterium Rhodospirillum rubrum KS-301 has been studied under cultural conditions. Bacterial growth showed a tendency to decline according to the degree of the concentration of butachlor until $10^{-3}$ M and slmost stopped at $10^{-2}$M. The growth inhibitory action at $10^{-3}$M of butachlor was evident (4.2-18.7% inhibition of growth rate) but had little effect in nitrogen fixation. Conversely, there was a little enhancement effect(1%) in pyruvate, dinitrogen gas growing cultures. At concentration of $10^{-3}$ M, instead of spiral form, rod shapes were observed through phase contrast microscope and instead of vesicular intracytoplasmic membrane, irregular tubular forms were observed through electron microscope. Alkaline pH value slightly reversed tha inhibitory action of butachlor.

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Associated Nitrogen Fixation in the Rhizosphere of Rice in Saline and Reclaimed Saline Paddy Soil -1. Enumeration of aerobic heterotrophic bacteria associated in histosphere of grasses and rice (간척지토양(干拓地土壤)의 수도근권(水稻根圈)에서 협생질소고정(協生窒素固定)에 관(關)한 연구(硏究) -제(第)1보(報) 수도(水稻) 및 자연생잡초(自然生雜草) 근조직내(根組織內) 협생질소고정균(協生窒素固定菌)의 분리동정(分離同定))

  • Lee, Sang Kyu;Suh, Jang Sun;Ko, Jae Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.1
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    • pp.69-76
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    • 1987
  • The aerobic heterotrophic bacteria in the histosphere associated with grasses (Gramineae, Caryphyllaceae, Crucifereae) and rice cultivars in saline and reclaimed saline paddy soils were varied with species and rice cultivars. The fraction of aerobic heterotrophic $N_2$-fixing bacteria to the total aerobic heterotrophic bacteria were averaged to eighteen percent in the histosphere of grasses and rice. Acetylene reducing activity of these bacteria were ranged from 1 to 24 n mole/tube/hr. Most of the bacteria strains were predominated of hydrogen utilizing bacteria. The majority of these bacteria were closed to Pseudomonas, Azospirillum, Klebsiella and Agrobacter.

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Changes of Nitrogen-Fixation Activity and Environmental Factors of Growth in Lespedeza bicolor Turcz (싸리(Lespedeza bicolor Turcz.)의 공생 질소고정활성과 생육환경요인의 변화)

  • 송승달
    • Journal of Plant Biology
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    • v.35 no.4
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    • pp.317-322
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    • 1992
  • The nitrogen fixation activity and environmental factors of Lespedeza bicli!oy Turcz, forming annual root nodules by symbiotic Rhizobium sp. were analyzed in the field conditions during the growing period. Seasunal changes of $N_2-fixation$ activity showed the maximum value of $120\;\mu\textrm{M}\;C_2_H4{\cdot}noduie\;g\;fw^{-1}{\cdot}hr^{-1}$ during the active growing period (June) and varied significantly depending on the growth phase and environmental factors. The maximum activities were attained at the conditions of pH 7, $30^{\circ}C$ of temperature, 18 Kpa of oxygen partial pressure and inhibited by water stress and nitrogen sources. The habitat soil was weak acidic and poor in nitrogen, phosphorus and organic matter contents. The leaf area ratios and chlorophyll contents were ranged from 442 to $48;\textrm{cm}^2{\cdot}g\;dw^{-1}$ and from 33 to $38\;\mu\textrm{g}\;chI{\cdot}\textrm{cm}^2$. Nitrogen contents in each organ showed the maximum of 46, 19 and $11\;mg{\cdot}g\;dw^{-1}, respectively for leaf, rool and stem in the early period. The highest phosphorus contents were 4.2, 1.2 and $0.6\;mg{\cdot}g\;dw^{-1}$, respectively for leaf. root and stem in early growing period. The allocation ratios of nitrogen quantity showed 60% for leaves and 73% for roots during the active and late growth period, and 22% [or stems in average. The allocalion ralios o[ phosphorus quantity showed 58% for leaves during the most productive period, 70% for roots in the pre-growth stage and 26% for stems in average.verage.

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PEG에 고정화된 Pseudomonas aeruginosa를 이용한 NH$_4$-N, NO$_3$-N 동시제거에 관한 연구

  • Park, Gyeong-Hun;Jeong, Gyeong-Hun;Choe, Hyeong-Il;Song, Won-Jong;Gang, Yeong-Ju
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2008.11a
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    • pp.503-506
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    • 2008
  • 질소제거 능력이 있는 Pseudomonas aeruginosa을 고분자물질인 PEG 에 포괄고정화하였으며 제조된 고정화 미생물을 이용하여 질소제거에 미치는 C/N비, 농도, 충진율, 탄소별 제거율을 검토한 결과 다음과 같은 결론을 얻었다. 1) C/N비 10이상이면 NH$_4$-N 와 NO$_3$-N의 동시 제거가 가능하였으며 2) 탄소 원으로는 glucose를 사용하였을 때 NH$_4$-N 와 NO$_3$-N의 동시 제거가 가능하였으나 methanol은 탄소원으로 사용할 수 없었다. 3) 저농도의 NO$_3$-N(50 mg/L)는 완전히 제거 가능하였으나 NH$_5$-N인 경우에는 초기 NH$_4$-N 100 mg/L에서 60%정도만 제거되었다. 4) 연속처리 결과 NH$_4$-N는 HRT 변동에도 불구하고 유출수 농도 변화가 거의 없었으나, 오히려 C/N비를 증가시키면 NH$_4$-N 제거 효율이 높았고, NO$_3$-N인 경우도 마찬가지로 C/N비를 증가시키면 NO$_3$-N 제거 효율이 높았다.

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Effect of Nitrogen, Phosphate and Cell Immobilization on Taxol Production from Cell Cultures of Taxus cuspidata (주목 (Taxus cuspidata) 세포배양에서 질소원, 인산, 세포고정화가 Taxol 생산에 미치는 영향)

  • Park, Jong-Hwa;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.38 no.4
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    • pp.308-312
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    • 1995
  • The effects of nitrogen, phosphate in modified B5 medium and cell immobilization on cell growth and taxol production were investigated using cell cultures of Taxus cuspidata. The ratio of nitrate to ammonium was found to be an important parameter. The ratio of 1 increased taxol production 10-fold, compared to the original ratio of 20 in modified B5 medium. Reducing phosphate concentration inhibited cell growth, but increased taxol production noticeably. Immobilized cells produced a taxol concentration of ${\sim}120\;g/l$

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Nitrogen fixation and componental changes in root nodules and plant organs during reproductive stages in soybeans. II. Nitrogenase activity and concentrations of total nitrogen, amino acid and allantoin-N in root nodules and plant organs (콩에 있어서 등숙기간중 질소도정작용과 근류 및 식물체 각 기관 체내성분 농도와의 관계. II. 식물체 각기관 총질소 Amino 산 및 Allantoin농도와 질소 고정능과의 관계)

  • Kim Seok Dong;Hong Eun Hi;Junji Ishizuka
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1986.06a
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    • pp.64-65
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    • 1986
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Genes Involved in Symbiotic Nitrogen Fixation (질소고정 공생관계 관련 유전자)

  • 안정선
    • Proceedings of the Botanical Society of Korea Conference
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    • 1987.07a
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    • pp.81-101
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    • 1987
  • In an attempt to revies the informations about genes involved in symbiotic nitrogen fixation, developmental processes in which host plant interact with microbe during nodule formation were introduced first. The structure, function and regulation of the genes discussed were mainly about microbial genes; those involved in the process of nodule formation (nod-genes) and of nitrogen fixation (nif-genes). Informations about the host genes involved in the symbiosis were discussed briefly.

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