• Korean Journal of Medicinal Crop Science
• /
• v.8 no.1
• /
• pp.9-13
• /
• 2000

To determine the proper carbon and nitrogen sources and their proper levels for mass micro propagation of Scrophularia buergeriana Miquel, tonic and curing cough experiment were applied and a method for mass cultivation by using bioreactors (2.5 L) was expinined. Proper ratio of $NH_4NO_3\;:\;$KNO_3$ was 413 mg/L : 1900 mg/L for multiple shoot production. Sucrose was more effective than glucose or fractose as carbon source and 3% concentration was good for shoot formation. Total nitrogen was not detected after six weeks both in 500 ml flask and bioreactor culture. Sucrose was decreased sharply after two weeks and there was no sucrose left after three weeks both in 500 ml flask and bioreactor culture. The stirrer in bioreactor caused shear stress to shoots severely. The sphere type bioreactor was better than the cylinder type and removal of inner loop in sphere type was more effective to avoid shear stress.

• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.2
• /
• pp.83-90
• /
• 2004

A pot experiment was conducted to find out the effects of nitrogen content of irrigation water and soil EC on lettuce growth under plastic film house conditions. The square-pots with 42 x 54.5 x 22 cm in length, width and height, were filled with two different soils of different EC. Lettuce was grown with different nitrogen fertilizer application levels including non fertilization (Non-F), decrement of 50% of nitrogen fertilizer recommended by soil testing (DNFRST-50) and fertilization recommended by soil testing (FRST). Two kinds of irrigation water of different nitrogen contents, $6.6mg\;L^{-1}$ and $21.0mg\;L^{-1}$, were used for the experiment. In the low EC soil irrigated with low nitrogen water, fresh weights of lettuce were 6,733, 11,933 and $12,733kg\;ha^{-1}$ for the treatments of Non-F, DNFRST-50 and FRST, respectively. While with high nitrogen water, the yields were 9,733, 13,400 and $12,800kg\;ha^{-1}$, respectively. In the high EC soil irrigated with low nitrogen water, lettuce yields of the Non-F, DNFRST-50 and FRST treatments were 12,400, 12,867 and $10,400kg\;ha^{-1}$, respectively, and with high nitrogen irrigated water lettuce yields of the Non-F, DNFRST-50 and FRST treatments were 13,600, 14,067 and $10,733kg\;ha^{-1}$, respectively. Nitrogen uptake of lettuce from ferilizer in DNFRST-50 was higher than of FRST. Nitrogen uptake of lettuce from irrgation water was found in soils of low EC, but it was not found in soils of high EC. These results suggest that both soil EC and nitrogen content of irrigation water should be considered when we recommend the level of fertilizer application for lettuce.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.12 no.3
• /
• pp.70-73
• /
• 1992

사료작물 저온발아성(低溫發芽性)은 수수 $\times$ 수단그라스잡종들이 좋았고 옥수수와 수단그라스는 비교적 저조하였고 PGE에 종자침지처리가 이탈리안라이그라스의 저온발아에 효과적이었다. 간척지의 수수파종시 염해 방지에는 모래를 파종후 피복하여 제염(除鹽) 효과를 얻을수 있었다. 수수류의 토양수분조건에 대한 광합성 연구는 포장용수량의 $60\sim80%$의 조건에서 광합성이 가장 왕성하였고 건물수량도 동일한 결과를 나타내었다. 옥수수의 광이용과 초형과의 관계연구는 이삭상부 엽각의 직립화는 밀식 조건에서 광이용성이 증가되었고 건물 및 종실수량에서 종수가 있었다. 또 밀식화에 따라 직립화 경향이 현저하였으며 옥수수의 다수성초형의 육성이 요청되었다. 질소시비에 대한 청예류의 생장 반응 연구들은 수단그라스에서 10a당 40Kg까지는 건물수량생산을 증가시켰으나 80Kg/10a 사용시는 질산태질소함량이 중독위험치를 초과하였다. 이러한 질산태질소의 중독위험은 6엽기까지 존재하였고 청산중독의 위험농도는 4엽기 이전까지, 또 18/$8^{\circ}C$의 저온조건에서 나타났다. 옥수수의 적정재식 밀도는 10a당 16.600본까지 밀식할수록 증가되었으나 가소화수량과 종실수량은 5.500본과 10Kg/10a 에서 최고치를 나타내었고 질소 추비 효과는 대관령 같은 고산지대에서는 나타나지 않았다. 맥류의 총체(總體)사료 이용성은 호밀, 밀, 보리 순(順)으로 저하하였고 파종시기가 빠를수록 월동성이 향상되었으며 수확적기는 출수후 24일경이었다. 호밀의 청예이용에는 월동후 2회예취와 12cm높이가 건물수량과 TDN수량을 높힐 수 있었다. 수수$\times$ 수단잡종의 출수기 이전 예취는 고사 경비율을 줄일 수 있었고 년 2회 예취와 예취높이 10cm가 가장 좋았다. 유채는 생육기간이 길어질수록 수량은 증가하였고 소화율의 감소도 현저하지 않았다.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.6
• /
• pp.1112-1117
• /
• 2011

This study was conducted to recommend nitrogen (N) top dressing based on soil nitrate content for leaf perilla under forcing culture in Gumsan-gun and Milyang-si. Experimental design was the randomized complete block design for five N fertilization levels and conventional fertilization. Dry weight, nitrogen uptake, and the node number of leaf perilla were measured and soil nitrate contents were analyzed monthly. The amount of nitrogen uptake for growth of a node with two leaves was $2.2kg\;10a^{-1}$ for Gumsan site and $3.5kg\;10a^{-1}$ for Milyang site. Lower level of soil nitrate N concentration for standard N fertilization was determined as $10mg\;kg^{-1}$ for both sites. Soil depth, bulk density, utilization rate of soil nitrate N, and the amount of N uptake for growth of a node with two leaves were considered for calculation of upper level of soil nitrate N concentration. The upper levels of soil nitrate N concentration for no N fertilization were determined as $30mg\;kg^{-1}$ for Gumsan site and as $40mg\;kg^{-1}$ for Milyang site. Consequently the recommendation equations for the N top dressing were Y=-0.157X+4.71 for Gumsan site and Y=-0.1667X+6.6667 for Milyang site.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.4
• /
• pp.392-399
• /
• 1998

Nitrogen fertilizers such as urea are readily hydrolyzed in soils to produce ammonium ions which pass through nitrification and denitrification processes. These serial processes have drawn attention due to nitrogen losses, eutrophication, blue baby syndrome, and ozone depletion problems. The purpose of this study was to test the inhibitory effects of hot-water extract and organic solvent fractions of Artemisia asiatica leaves on soil urea hydrolysis and nitrification. In addition, the effects of organic solvent fractions on urease activity and ureolytic bacterial population were also investigated. First, hot-water extract of Artemisia asiatica leaves inhibited soil nitrification substantially with a marginal stimulatory effect on soil urea hydrolysis. Soils treated with hot-water extract of Artemisia asiatica leaves showed significant decreases in the accumulation of soil $NO_3-N$ (~68% decrease) compared with the control soil without the treatment of hot-water extract. In contrast, $CHCl_3$/MeOH fraction and basic aqueous layer of Artemisia asiatica leaves inhibited soil urea hydrolysis very strongly, causing 5.8 and 4.3-fold higher accumulation in amounts of remaining urea-N compared with the non-treated soil. Meanwhile, non of the organic solvent fractions showed any significant effects on soil nitrification inhibition. The inhibition of ureolytic bacterial activity by $CHCl_3$/MeOH fraction and aqueous basic layer of Artemisia asiatica leaves without any effects on urease activity itself led us to conclude that the inhibitions of soil urea hydrolysis were caused by the antagonistic effects on ureolytic bacterial activity.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.19 no.3
• /
• pp.203-210
• /
• 1999

Of all the nutrients in liquid manure, N has the greatest potential both for the environment and for increasing forage yields. This experiment was carried out to determine the effect of different types and N rates of liquid manure on mineral content and nitrate-N of oats(Avena sativa L.), soil chemical characteristics in Suweon. Seven treatments consisting of chemical fertilizer $120kg\;N\;ha^{-1}$, liquid cattle manure 120, 240 and $360kg\;N\;ha^{-1}$, liquid swine manure 120, 240 and $360kg\;N\;ha^{-1}$ were arranged in a randomized complete block design with three replications. Compared with the plot of chemical fertilizer, increasing liquid manure N rates increased mineral contents of oats. Nitrate-N contents of oats were ranged from 1,881 to $2,605mg\;kg^{-1}$ in all treatments, which was orderly ranked as chemical fertilizer>liquid cattle manure $360kg\;N\;ha^{-1}$> liquid swine manure $240kg\;N\;ha^{-1}$. Contents of exchangeable cation of the soil were appeared to be higher with increasing liquid manure N rates. Amount of total-N and inorganic-N in soil affected by increasing liquid manure N rates, and there was the highest at liquid swine manure $360kg\;N\;ha^{-1}$ among the treatments. Nitrate-N concentration in infiltration water was not remarkably variable during the experimental period. Based on the results of this experiment, it is suggested that the amount of nitrogen in soil was orderly ranked as liquid swine manure $360kg\;N\;ha^{-1}$, followed by liquid swine manure $240kg\;N\;ha^{-1}$.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.2
• /
• pp.187-192
• /
• 1997

We carried out a survey on tributary streams in some agricultural areas along Nakdong River to evaluate the effects of agricultural practices on pollution of stream water and groundwater. General properties, nutrient materials and heavy metals in water samples were measured. General physicochemical properties of tributary stream waters were at levels favorable for agricultural water. Heavy metals, except Zn, were mostly not detected. Total-N contents were much higher than the criteria of agricultural water, and nitrate-N accounts for more than a half of total-N. Phosphorus contents were higher than the lower level of P for algae growth and the contents were high especially in summer. In ground waters which are used for irrigation, P were mostly at same levels as those in streams, and nitrate contents were higher than 10mg/L in some samples. In these results only those N and P contents in stream and ground waters higher than pollution criteria are problematic and they are traceable to agricultural nonpoint sources-fertilizers, livestock farms and sewage. Further researches are needed to evaluate contributions of each nonpoint source on stream water pollutions.

• Korean Journal of Environmental Agriculture
• /
• v.21 no.3
• /
• pp.197-201
• /
• 2002

This study was conducted in Jeju Island to find the effects of livestock manure application on the changes in soil salt concentration and $NO_3-N$ contents. Soil samples were collected from Goojua-Tong (volcanic ash soil) and Aewol-Tong(non-volcanic ash soil) to 50 cm depth and were mixed with livestock manure to 20 cm depth in PVC container(30 cm diameter, 1 m height). Animal manures of cattle, pig, and fowl were adjusted to 0, 50, 100,150 ton/ha. Animal manure applications increased the salt concentrations in soil. The salt concentration was increased as the fowl manure amount was increased The effects were larger in order of fowl manure > cattle manure $\fallingdotseq$ pig manure. $NO_3-N$ contents in soil showed a sharp increase by applications of fowl manure, but the increase was slow when the cattle and pig manures were applied. In volcanic ash soil, there was no change in phosphate contents by application of animal manures, but the phosphate contents increased in non-volcanic ash soil with the application of animal manure, especially by fowl manure.

• Korean Journal of Medicinal Crop Science
• /
• v.1 no.1
• /
• pp.58-62
• /
• 1993

The effects of carbohydrate sources(sucrose, glucose) and ratio of ammonia to nitrate on essential oil production and its com- positions in the callus culture of Mentha piperita L. were studied. An ammonia : nitrate ratio of 1 :2 was more effective for essential oil production regardless of the media used ; Lin-Staba(LS) and modify Murashige-Skoog(MS) medium. Menthol biosynthesis was enhanced when ratio of ammonia to nitrate was 1 :3 in the LS medium while the ratio was 1 :2 in the MS medium. Lower sucrose concentration(20g /1 ) was much better than higher sucrose concentration(30g /1) for both oil and menthol biosynthesis in the LS medium but higher sucrose concentration(30g /1) was more effective for those in the MS medium. When sucrose was replaced with glucose, menthol biosynthesis was sharply decreased or absent regardless of media used.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.2
• /
• pp.189-196
• /
• 1998

Swage sludge cakes produced from domestic wastewater treatment plants were collected from 21 different sites throughout Korea, and chemical properties of the sludge samples were determined. Inorganic nutrient contents did not indicate great differences among swage sludges from each sites, whereas the toxic heavy metal contents differed greatly. T-N, $NH_4{^+}-N$ and $NO_3{^-}-N$ contents from 21 sites sludges ranged 2.3-6.0, 291-4284, $1.4-58.8mg\;kg^{-1}$, respectively. Heavy metal (Cd, Cu, Pb and Zn) contents ranged 2.86-58.22, 144.0-5417.3, N.D.-943.5, and $N.D.-8,083mg\;kg^{-1}$, respectively. One of the sludges was treated to soils at rates of 12.5, 25, 50. and 100, $Mg\;ha^{-1}$ and incubated for 12 weeks to determine nitrogen materialization rate. Ammoniun nitrogen content decreased sharply at higher rates of sludge treatment up to 8 weeks after treatment and did not change much, while $NO_3{^-}$ increased at all treatment levels. The net amount of mineralized N of sludge treatment rates (12.5, 25, 50, and $100Mg\;ha^{-1}$) during 12weeks incubation were 189.0, 277.2, 303.8 and $376.6mg\;kg^{-1}$.