• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.124-130
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• 2021

Nitrate is an important nutrient and signaling molecule in plants that modulates the expression of many genes and regulates plant growth. In this study, we cover the research status of transcription factors related to the control of gene expression by nitrate signaling in higher plants. Nitrate reductase is a key enzyme in nitrogen assimilation, as it catalyzes the nitrate-to-nitrite reduction process in plants. A variety of factors, including nitrate, light, metabolites, phytohormones, low temperature, and drought, modulate the expression levels of nitrate reductase genes and nitrate reductase activity, which is consistent with the physiological role if. Recently, several transcription factors controlling the expression of nitrate reductase genes have been identified in higher plants. NODULE-INCEPTION-Like Proteins (NLPs) are transcription factors responsible for the nitrate-inducible expression of nitrate reductase genes. Since NLPs also control the nitrate-inducible expression of genes encoding the nitrate transporter, nitrite transporter, and nitrite reductase, the expression levels of nitrate reduction pathway-associated genes are coordinately modulated by NLPs in response to nitrate. Understanding the function of nitrate in plants will be useful to create crops with low nitrogen use.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.339-339
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• 1998

• Journal of the Korean Society of Food Science and Nutrition
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• v.10 no.1
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• pp.47-52
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• 1981

Changes of nitrate and nitrite contents in various parts (cotyledon, hypocotyl, root) of germinating soybean were studied in relation to activities of nitrate reductase (NR). The optimal pH and temperature of the NR were around pH 6.5 and $30^{\circ}C$, respectively. The stable pH range of the enzyme was between pH 4.0 to 6.5. The enzyme was stable at $40^{\circ}C$. Activities of NR in each part of germinating soybean increased in order of root, hypocotyl and cotyledon. Hypocotyl and root were maximum activity around 10 days after germination, and cotyledon around 9 days. Relationship between nitrate contents and NR activities was observed except the middle period of germination, and that of nitrite contents and NR activities was partially observed.

• Applied Biological Chemistry
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• v.45 no.3
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• pp.129-133
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• 2002

Suppression of nitrate accumulation in spinach and lettuce through foliar application of chitosan formula containing micronutrients is related with the increase of the nitrate reductase (NR) activity. If NR in spinach were highly expressed to increase the assimilatory activity, nitrate content could be reduced. For this, NR cDNA was cloned from the isolated mRNAs of spinach using reverse transcriptase-PCR. Nucleotide sequence of cloned spinach NR cDNA showed highly deduced amino acid sequence identity ($71{\sim}82%$) with other known plant NR genes. Only two nucleotide-base differences were observed in the cloned NR cDNA compared with that of the published spinach NR cDNA.

• Journal of the Korean Society of Food Science and Nutrition
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• v.10 no.1
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• pp.39-45
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• 1981

The content of nitrate and nitrite in germinating soybean was studied in relation to ascorbic acid contents. Nitrate content during soybean germination gradually increased, and root was higher than cotyledon and hypocotyl. Nitrite content was relatively low and maintained under the 1 ppm during germination period. Ascorbic acid content was maximum around 4 days after germination.

• Journal of Plant Biology
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• v.34 no.4
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• pp.283-288
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• 1991

This work was carried out to determined the effect of 2,4-dinitrophenol(DNP) on in vivo nitrate reductase activity in the root of 6 day old rye (Secale cereale L.) seedlings. The nitrate reductase activity in the roots of 6 day old rye seedlings pretreated with 0.5 mM DNP was higher than that of the control group in all the experimental conditions. The optimal concentration of KNO3 for maximum nitrate reductase activity was 10 mM in both control and treated group. The nitrate reductase activity in the treatment of 10 mM KNO3 gradually increased for 4 h in both groups, and then maintained constantly. The nitrate reductase activity occurred per hour was highest at 1 h in both groups, while it was declined by large degrees as time goes on. The daily pattern of nitrate reductase activity was gradually decreased in both groups with the passage of day. The optimal pH for this experiment and a previous paper (Kwon et al., 1991), it was determined that the nitrate reductase activity in both roots and shoots of rye seedlings was increased by the treatment of 0.5 mM DNP, and particulary in both groups, the nitrate reductase activity in the roots of rye seedlings was higher than that in shoots of them.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.69-73
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• 2019

During screening of microbes for compounds having cosmetic benefits, we isolated Marinobacter salarius HL2708#2 from lava seawater on Jeju Island, Republic of Korea. The complete genome sequence was determined. Strain HL27080#2 features a circular chromosome of 4,304,603 bp with 57.21% G+C content and a 244,163 bp plasmid with 53.14% G+C. There were 4,180 protein coding sequences identified, along with 49 transfer RNA and 18 ribosomal RNA noncoding genes. The genome harbored genes for the utilization of alcohol, maltose/starch, and monosaccharide as sole carbon sources. Genes responsible for halophilic characteristics and heavy metal resistance could be annotated, as well as aromatic and alkane hydrocarbons. Contrary to the prior report that M. salarius is negative for nitrate and nitrite reduction, nitrate/nitrite reductase along with nitrate/nitrate transporters and nitronate monooxygenase were evident, suggesting that strain HL2708#2 may be able to denitrify extracellular nitroalkenes to ammonia.

• Horticultural Science & Technology
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• v.18 no.1
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• pp.14-17
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• 2000

This experiment was carried out to investigate the effect of nitrogen form in the Hoagland nutrient solution on the growth of Farfugium japonicum, Aster tataricus and Chrysanthemum boreale. The nitrogen form of Hoagland nutrient solution was modified to $NH_4{^+}(100%),\;NO_3{^-}(100%),\;and\;NH_4{^+}+NO_3{^-}(50%+5%)$ for this study. In the treatment of $NO_3{^-}\;and\;NH_4{^+}+NO_3{^-}(1:1)$, F. japonicum showed the best growth, especially in fresh weight. With the treatment of $NO_3{^-}$, shoot and root fresh weight of A. tataricus and C. boreale were increased. The activity of nitrate reductase and the concentration of chlorophyll and nitrate were increased with $NO_3{^-}$ treatment in all these plants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.11 no.3
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• pp.15-19
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• 1982

The Changes of nitrate and nitrite contents at different storage temperatures (low tempenature : $4{\sim}7^{\circ}C$, high temperature: $28{\sim}30^{\circ}C$) were studied in relation to the viable bacterial population. The contents of nitrate and nitrite in milk were 1. 10 ppm and 0.03 ppm, respectively. When milk was storaged for 7 days at $28{\sim}30^{\circ}C$, nitrate concentration decreased to 0.3 to 0.6 ppm, whereas nitrite increased up to 0.13 to 0.08 ppm. However, during the storage at $4{\sim}7^{\circ}C$, their contents were little changed. The number of viable bacterial population in raw milk appeared $9{\times}10^6/ml$, and rapidly increased throughout 3 days storage at high temperature, but afterward it tended to decrease. This seemd to indicate that nitrate was reduced to nitrite by the nitrate reducing bacteia in milk, and the changes in the viable bacterial population were also related to the changes of pH and titratable acidity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.2
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• pp.103-110
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• 2009

Rape plants, especially forage type, are known as one of high S-demanding plants. Their productivity and quality have often limited under S-deficient condition. To investigate the effect of S-deficiency on N uptake and its assimilation, $NO_3^-$ absorption, nitrate reducatse (NR) and glutamine synthetase (GS) activity in leaf and root tissues as affected by different S-supplied level was determined. $NO_3^-$ uptake was not significant between control and S-deficient treated plants, while significantly depressed in S-deprived plants for the early 8 h. NR activity decreased as S-availability decreased, especially in young and middle leaves, representing more than 35% of decrease in S-deficient and 70% in S-deprived plants when compared with control. In roots, a significant decrease (-29%) in NR was observed only in S-deprived plants. Relatively higher GS activity was found in young leaves for three all treatments. As a whole leaf tissue, S-limited conditions resulted in a reduction of GS activity. In root which showed the lowest activity, a significant decrease (-30%) was observed only in S-deprived plants.