• Korean Journal of Microbiology
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• v.47 no.2
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• pp.103-109
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• 2011

Pectobacterium carotovorum subsp. carotovorum is the causative agent of soft rot in crops such as potato and cabbages. Loop-mediated isothermal amplification (LAMP) is a simple DNA amplification method, as well as isothermal PCR technique. In this study, a new method for the rapid detection of Pectobacterium carotovorum subsp. carotovorum was developed using LAMP that named PCC-LAMP. Based on lytic murein transglycolase gene of Pectobacterium carotovorum subsp. carotovorum, a set of four primers for LAMP was designed. The optimal PCC-LAMP reaction temperature was established at $61^{\circ}C$. Under standard conditions, PCC-LAMP amplified $1{\times}10^3$ copies of clone PCC-pBX437 per reaction. Further, this method can also assay directly by SYBR Green I without electrophoresis. Amplification was not detected for five other bacterial species. In conclusion, PCC-LAMP may be a useful method for the detection Pectobacterium carotovorum subsp. carotovorum in the field.

• Laboratory Medicine Online
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• v.7 no.1
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• pp.13-19
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• 2017

Background: We evaluated a sensitive and quantitative method utilizing fragment analysis of the fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD), simultaneously measuring mutant allele burden and length, and verified the analytical performance. Methods: The number and allelic burden of FLT3-ITD mutations was determined by fragment analysis. Serial mixtures of mutant and wild-type plasmid DNA were used to calculate the limit of detection of fragment analysis, conventional PCR, and Sanger sequencing. Specificity was evaluated using DNA samples derived from 50 normal donors. Results of fragment analysis were compared to those of conventional PCR, using 481 AML specimens. Results: Defined mixtures were consistently and accurately identified by fragment analysis at a 5% relative concentration of mutant to wild-type, and at 10% and 20% ratios by conventional PCR and direct sequencing, respectively. No false positivity was identified. Among 481 AML specimens, 40.1% (193/481) had FLT3-ITD mutations. The mutant allele burden (1.7-94.1%; median, 28.2%) and repeated length of the mutation (14-153 bp; median, 49 bp) were variable. The concordance rate between fragment analysis and conventional PCR was 97.7% (470/481). Fragment analysis was more sensitive than conventional PCR and detected 11 additional cases: seven had mutations below 10%, three cases represented conventional PCR failure, and one case showed false negativity because of short ITD length (14 bp). Conclusions: The new fragment analysis method proved to be sensitive and reliable for the detection and monitoring of FLT3-ITD in patients with AML. This could be used to simultaneously assess ITD mutant allele burden and length.

• The Korean Society of Law and Medicine
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• v.22 no.3
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• pp.97-124
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• 2021

In medical institutions, there are radiation-related workers such as radiological technologists, physicians, dentists, and dental hygienists who handle diagnostic radiation generators. Also, there are work assistants, such as nurses and assistant nurses, who assist in radiation treatment or transfer patients to the radiation examination room. Radiation exposure management for radiation-related workers is carried out under the 「Medical Service Act」, but there is no legal basis for work assistants, etc. And the management of radiation exposure for diagnosis is regulated by the 「Medical Service Act」, and the management of radiation exposure by therapeutic radiation and nuclear medical examination is governed by the 「Nuclear Safety Act」. Thus, to improve the management of radiation exposure for diagnosis, the regulations on radiation exposure management for diagnosis under the 「Medical Service Act」 were compared and reviewed with those of the 「Nuclear Safety Act」. As a result, the main contents are as follows. First, it is necessary to legislate to include nurses, assistant nurses, and clinical practice students who are likely to be exposed to radiation besides radiationrelated workers as subjects of radiation exposure management for diagnosis. Second, when a radiation-related worker for diagnosis is confirmed to be pregnant, the exposure dose limit should be defined. Third, it is necessary to revise the regulations on the types of personal exposure dosimeters in the 「Rules on the Safety Management of Radiation Generators for Diagnostics」. Fourth, it seems that health examination items for radiation-related workers, radiation workers, and frequent visitors should be the same. Fifth, It is necessary to unify and regulate diagnostic radiation and all medical radiation, including therapeutic radiation and nuclear medicine, in one legal system.

• Food Science of Animal Resources
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• v.29 no.4
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• pp.533-538
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• 2009

To suggest an improved diagnostic method for Salmonella spp., analyses were conducted with immunoliposomes and compared with the results from a commercial test kit. One sample out of 36 samples of eggshell was Salmonella-positive via immunoliposomes. In the case of the use of the commercial test kit, six samples out of 36 samples were Salmonellapositive. These Salmonella-positive samples were subjected to biochemical identification tests that confirmed that they were Salmonella-negative. As for the egg content samples, they were Salmonella-negative in both analyses with immunoliposomes and the commercial test kit. The Salmonella analysis with immunoliposomes reduced detection time, by 24 h compared to the commercial test kit. Bacteria, including Acinetobacter baumanni, Chryseomonas luteola, Enterobacter cloacae, Escherichia coli, Escherichia hermannii, Klebsiella pneumonia, Pantoea spp., and Pasteurella pneumotropica, were isolated from the eggshells. Other than Acinetobacter baumanni and Pasteurella pneumotropica most of the isolates were known to frequently appear during egg production processing.

• The Korean Journal of Applied Statistics
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• v.15 no.1
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• pp.139-151
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• 2002

This study is concerned with model selection and diagnostics for nonlinear regression model through Bayes factor. In this paper, we use informative prior and simulate observations from the posterior distribution via Markov chain Monte Carlo. We propose the Laplace approximation method and apply the Laplace-Metropolis estimator to solve the computational difficulty of Bayes factor.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.15 no.7
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• pp.581-601
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• 1990

A variety of method to diagnose the fault of rotational mechine is suggested, the latest data. This paper uses Bispectrum which is sort of high order spectrum, diagnose the ball bearing of the rotational machine element. Apprehending the physical meaning of Bispectrum, computer simulation is performed. The result from computer simulation and the signal of the faulted ball bearing is studied from all it's aspects. It is found that the Bispectrum is more effective than the conventional Power Spectrum.

• Electronics and Telecommunications Trends
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• v.29 no.5
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• pp.105-113
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• 2014

주치의에게는 암환자의 암 전이 여부가 초미의 관심사다. 암환자의 10명 중 9명이 전이암으로 사망하기 때문이다. 암의 전이 초기에 그 전이 여부를 방사선 진단법으로 가능하지 않다. 혈액을 채취하여 암의 전이 유무를 진단 하는 기술이 개발되고 있다. 이 혈중 암세포는 혈구세포 10억개당 1~100개 정도의 극히 미량이 존재하여 암세포 분리기술이 특별히 잘 개발되어야 한다. 최근 마이크로바이오칩 형태의 분리기술이 큰 기술적 진화를 보이고 있어 본고에 소개하고자 한다. 이 기술은 한 가지 큰 의미를 갖는다. 그것은 암환자의 암 전이 모니터링에 필요한 도구가 될 수 있기 때문이다. 전이암세포 검출 키트로 전이암세포를 계수 하여 환자에게 투약한 항암제가 적합한지에 대한 답을 의사는 얻을 수 있다. 전이암세포 진단용 마이크로바이오칩 기술이 기존의 영상진단법만큼 중요한 임상 수단이 될 것으로 전망된다.

• Journal of Biomedical Engineering Research
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• v.16 no.4
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• pp.555-561
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• 1995

In this paper, the O-Ring Measurement System (O-R MS) has been designed to supplement such subectivity and apply to the diagnosis by constitution. A single chip microprocessor of Intel MCS-51 family has been used to control the O-R MS and process data with real time, and the O-R MS could measure the minute muscular strength, expanded width between fingers, and time of the measurement. Therefore the objective constitutional diagnosis is possible by the O-R MS. As a clinical testing for estimating credibility of O-R MS, the acupuncture points representing the constitution has been stimulated using a semiconductor laser stimulator and measure the muscular strength by the O-R MS. The result of clinical testing has been shown that the constitutional diagnosis with the O-R MS is proved highly precision of 96% and it is expected that the O-R MS can be used practically for the objective constitutional diagnosis.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.94.2-94.2
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• 2016

플라즈마 분광진단 기술은 기존 프로브와 달리 플라즈마에 섭동을 일으키지 않고, 전자온도, 밀도와 같은 플라즈마의 물리적 특성 진단과 함께 라디컬의 밀도와 같은 플라즈마의 화학적 특성을 진단할 수 있는 기술로 각광을 받고 있다. 본 발표에서는 레이저진단의 고급 진단을 제외한 플라즈마 변수 측정을 위한 플라즈마 방출 스펙트럼을 이용하는 방출분광진단과 흡수 스펙트럼을 이용한 흡수분광 진단에 대한 소개와 함께, 이를 이용한 플라즈마의 전자여기온도, 전자밀도, 전자회전온도, 기체온도 및 중성종의 절대밀도 온도 측정에 대한 기술과 실례를 소개한다.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.256.1-256.1
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• 2014

21세기 정보화 시대의 도래와 함께 반도체 및 디스플레이 분야는 고부가가치산업으로 급격히 성장하였고, 현재까지도 미래의 지속적인 시장 창출을 위하여 기술개발과 투자로 초미세화, 고효율, 대면적화에 대한 원천기술 확보가 중요시되고 있다. 반도체 및 디스플레이의 대면적화가 진행됨에 따라 플라즈마 공정장비의 대면적화도 활발히 기술개발이 진행되고 있으며, 대면적화에 있어 플라즈마의 공간균일도는 생산수율 및 공정균일화를 위해 기본적으로 평가되어야 하는 중요한 지표가 되었다. 하지만 종래의 진단법들은 대면적 플라즈마 진단에 매우 제한적이기 때문에 본 연구에서는 대면적 플라즈마의 공간균일도 평가를 위해 플라즈마의 방출광 측정을 기초로 하는 진단계를 개발하였다. 플라즈마 방출광을 이용한 진단은 플라즈마에 섭동을 주지 않고 전자온도의 변화 및 공간균일도를 평가할 수 있다. 이 진단법은 두 마주보는 한쪽 면이 평평한 볼록렌즈(plano-convex lens)로 이루어진 수광시스템과 역변환 알고리즘을 통해 선 적분된 방출광으로부터 플라즈마 방출광의 국지적 정보를 측정하는 것이다. 플라즈마와 같이 크기가 큰 광원의 경우 렌즈 광학계에서 필연적으로 수반되는 선적분된(chord-integrated) 방출광을 제거하기 위해 구조에 따른 시스템 함수를 이용한 푸리에 변환 알고리즘을 개발하였고, 이를 통해 렌즈 초점거리의 정확한 방출광 세기만 재구성하였다. 이러한 재구성 방법을 이용하여 렌즈의 거리를 움직이며 대면적 플라즈마의 방출광 분포측정을 수행하였고, 이에 대한 결과를 발표하고자 한다.