• 제목/요약/키워드: 지표미생물

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Microbial Analysis of Processing and Evaluation of Shelf life of Fried Bean Curd (유부의 가공공정중 미생물 분석과 저장 수명 평가)

  • 노우섭
    • Journal of Food Hygiene and Safety
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    • v.13 no.1
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    • pp.62-67
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    • 1998
  • This study was undertaken to find out distribution and contamination sources of microbes on the processing steps and to estimate quality index and shelf life of fried bean curd. It was necessary that the sanitation for water, processing environment and instruments at digestion, formation, cutting and processes after frying must be controlled and microbial growth at digestion and formation must be inhibited, to process efficiently and to improve shelf life of fried bean curd. It was evaluated that quality indexes as to sensory evaluation, especially texture, mold generation and total viable cell counts will be useful to estimate shelf life of fried bean curd and that shelf life of fried bean curd was 6 days.

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Evaluation of MTBE-Contaminated Soil by Soil Enzyme Assay (Soil Enzyme Assay에 의한 MTBE오염 토양 평가)

  • 이은정;안윤주
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.211-214
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    • 2004
  • MTBE로 오염된 토양에서 생태독성학적 접근 방법으로 3가지 토양 효소의 활성도를 측정해 보았다. MTBE의 잠재적 위험성으로 인한 논란은 계속되고 있으나 토양 오염에 대한 지표로써 토양 효소 활성도의 사용타당성 여부에 대한 실험은 이전에 행해지지 않았다. 따라서 중금속 오염 토양에 대해 좋은 지표로 사용되고 있는 토양 미생물 효소의 활성도를 MTBE에 적용하여 실험해 보았다. 사용한 토양 효소는 Acid Phosphotase, $\beta$-Glucosidase 그리고 Arysulfatase였다. 그러나 실험 결과 MTBE로 오염된 토양의 경우 중금속으로 오염된 토양에 비해 토양 미생물 활성도의 감소가 매우 적었다. 따라서 MTBE의 오염 토양의 경우 본 연구에서 측정된 효소의 활성도는 좋은 지표로 적합하지 않다는 것을 확인했다.

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Thermophilic Bacillus Species as a Microbial Indicator of the History of Compost Application (부숙퇴비 시용내력 지표미생물로서의 고온성 Bacillus)

  • Suh, Jang-Sun;Yeon, Byeong-Yeol
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.285-290
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    • 1998
  • Thermophilic Bacillus species was studied as a microbial indicator to pursue the compost a application history. Thermophilic Bacillus species could be easily determined by the plate culture method within 12 hours in $65^{\circ}C$ incubator. The density of thermophilic Bacillus species in soils was gradually increased with the application rate of rice straw compost, and correlated to the soil organic matter content in $R^2=0.835^{**}$(n=32) coefficient on the 43-year-long term rice paddy fields.

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Sustainability Indices (=Green Star) for Microbial Fuel Cell (미생물 연료전지 영속발전 지표개발)

  • Song, Ha-Geun;KOO, Ja-Kong
    • Journal of the Korea Organic Resources Recycling Association
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    • v.23 no.2
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    • pp.47-52
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    • 2015
  • A microbial fuel cell (MFC) is a device that can be obtained electricity from a variety of organic through the catalytic reaction of the microorganism. The MFC can be applied to various fields, and research is required to promote the performance of the microbial fuel cell for commercialization. The lower performance of an MFC is due to oxygen reduction at the cathode and the longer time of microbial degradation at anode. The MFC amount of power is sufficient but, in consideration of many factors, as a renewable energy, now commonly power density as compared to Nafion117 it is an ion exchange membrane used is PP (Poly Propylene) from 80 to about 11 fold higher, while reducing the cost to process wastewater is changed to a microporous non-woven fabric of a low cost, it may be energy-friendly environment to generate electricity. All waste, in that it can act as a bait for microorganisms, sustainability of the microbial fuel cell is limitless. The latest research on the optimization and performance of the operating parameters are surveyed and through the SSaM-GG(Smart, Shared, and Mutual- Green Growth) or GG-SSaM(Green Growth - Smart, Shared, and Mutual) as the concept of sustainable development in MFC, the middle indices are developed in this study.

Seasonal distribution of microorganisms in the effluent water in various sewage treatment plants (하수처리장 방류수의 계절별 미생물분포에 관한 연구)

  • Kim, Young-Jun;Chung, Myung-Hee;Chung, Doo-Young;Lee, Hye-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.1
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    • pp.53-61
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    • 2008
  • Seasonal distribution of various microorganisms was investigated in the effluent water in various sewage treatment plants from January to November in 2007. The target microorganisms were composed of total heterotrophic bacteria, Escherichia coli, coliform and enteric bacteria, Staphylococcus aureus, Yeast and Mold. The number of total bacteria and the indicator microorganisms, such as E. coli, coliform, and Enterobacteriacea, were increased during the summer season. S. aureus, however, was not influenced by the weather, showing higher number even in November and January in most sewage treatment plants investigated. Meanwhile, disinfection methods employed in various plants had no direct relationship with the growth of microorganisms, and small-scaled plants were found to be more effective than large-scaled ones in the sterilization of microorganisms.

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Comparative Microbiome Analysis of and Microbial Biomarker Discovery in Two Different Fermented Soy Products, Doenjang and Ganjang, Using Next-generation Sequencing (차세대 염기서열 분석법을 이용한 된장과 간장의 미생물 분포 및 바이오마커 분석)

  • Ha, Gwangsu;Jeong, Ho Jin;Noh, Yunjeong;Kim, JinWon;Jeong, Su-Ji;Jeong, Do-Youn;Yan, Hee-Jong
    • Journal of Life Science
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    • v.32 no.10
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    • pp.803-811
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    • 2022
  • Despite the importance of traditional Korean fermented foods, little is known about the microbial communities and diversity of fermented soy products. To gain insight into the unexplored microbial communities of both Doenjang (DJ) and Ganjang (GJ) that may contribute to the fermentation in Korean traditional foods, we carried out next-generation sequencing (NGS) based on the V3-V4 region of 16S rDNA gene analysis. The alpha diversity analysis results revealed that both the Shannon and Simpson diversity indices were significantly different between the two groups, whereas the richness indices, including ACE, CHAO, and Jackknife, were not significant. Firmicutes were the most dominant phylum in both groups, but several taxa were found to be more abundant in DJ than in GJ. The proportions of Bacillus, Kroppenstedtia, Clostridium, and Pseudomonas and most halophiles and halotolerant bacteria, such as Tetragenococcus, Chromohalobacter, Lentibacillus, and Psychrobacter, were lower in DJ than in GJ. Linear discriminant effect size (LEfSe) analysis was carried out to discover discriminative functional biomarkers. Biomarker discovery results showed that Bacillus and Tetragenococcus were identified as the most important features for the classification of subjects to DJ and GJ. Paired-permutational multivariate analysis of variance (PERMANOVA) further revealed that the bacterial community structure between the two groups was statistically different (p=0.001).

Microbial Diversity, Survival and Recovery as Bioindicators in Soils from Different Parent Materials in Korea (생물학적 토양 지표로서의 모재별 미생물의 다양성과 복원율)

  • Suh, Jang-Sun;Kwon, Jang-Sik;Kim, Sang-Hyo
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.4
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    • pp.243-252
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    • 2002
  • To develop indicators for soil health evaluation, biological characteristics of native soils from the different parent materials were studied. Survival rate of fluorescent Pseudomonas in soils was the lowest as 0.1% while those of thermophilic Bacillus and alkaliphilic bacteria were over the 90% by the soil drying stress. There was positive relationship between soil microbial biomass and organic carbon exudated from the microbial biomass by the treatment. The average air-drying effect of soils was 39.7% with ranges of 9.7~95.0%. The propagules of mesophilic Bacillus and Gram negative bacteria were increased by the re-wetting of dried soils. Soil pH affected positively to the recovering rate of microbial number. Average recovering rate of microbes was 65.3%, and there was positive relationship between microbial biomass recovery and fluorescent Pseudomonas population.

Discharge Characteristics of the Indicator Microorganisms of Combined Sewer Overflows (합류식 하수관거 월류수의 지표미생물 배출 특성)

  • Kim, Geonha
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.4
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    • pp.627-635
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    • 2006
  • Combined sewer overflow (CSOs) is a primary diffuse source degrading water quality of urban streams. In this study, CSOs caused by 5 different rainfall events at an urban watershed located in Daejeon city were monitored for the indicator microorganism concentrations. Event mean concentration (EMC) of the indicator microorganisms were: total coliform = $2.46{\times}10^6CFU/100mL$; fecal coliform = $1.01{\times}10^6CFU/100mL$; E.coli = $5.20{\times}10^5CFU/100mL$; and Fecal Streptococci = $6.08{\times}10^5CFU/100mL$. In addition, coliform concentrations were well correlated with suspended solid concentrations and the first flush effects were identified. Settling tests were carried out to estimate removal rate of indicator organisms by sedimentation from CSOs. As microorganisms are discharged in association with suspended solid, ten minutes of settlement can lower 44% of indicator microorganism leading.

Evaluation of Bacteriological Safety in Deep Confined Aquifer (심층피압지하수에서 세균군집의 분석에 의한 분변성 오염 평가)

  • 조장천;김상종
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.47-52
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    • 1999
  • The study on the bacterial distribution was performed to evaluate the bacteriological safety in 4 deep confined aquifers drilled for drinking. All the investigated aquifers were oligotrophic, howevec bacterial densities and activities and the number of indicator bacteria in C-2 boreholes were much higher than those in other boreholes. Fecal pollution was observed in 3 boreholcs except C-1 borehole. The ratio of gram positive bacteria of the C-2 borehole was much lower than those 01 another boreholes. The numbers of total colifonns, fecal coliforms, and fecal Slreptococcus in the C-2 boreholes were 61, 33, 55 CFU (250 ml)$^{-1}$

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Microbiological and Organoleptic Qualities of Boiled-Dried Anchovies during Post-Irradiation Period (감마선 조사 건멸치의 미생물학적 및 관능적 품질안정성)

  • 권중호;변명우;김정숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.2
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    • pp.283-287
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    • 1996
  • 건멸치의 품질안정성 연구를 위하여 접합필름(nylon $15\mu\textrm{m}/polyethylene$ $100\mu\textrm{m})으로$ 포장된 시료에 감마선을 조사한 다음 상온과 $저온(5~10^{\circ}C)에$ 1년간 저장하면서 미생물학적 및 관능적 품질을 평가하였다. 건멸치에 오염된 미생물의 방사선감수성은 위생지표 미생물>호염성세균>효모 및 곰팡이의 순으로 나타났으며, 5kGy 범위의 감마선 조사는 저온 및 상온에서도 미생물학적 품질을 1년 이상 유지할수 있었다. 관능적품질에 있어서 시료의 겉모양(갈변)과 맛 기호성은 10kGy의 고선량 조사시 대조군보다 유의적으로 저하되었다(p<0.05). 접합필름 포장시료를 5kGy 조사하여 상온과 저온에 1년간 저장하였을 때 관능적 품질은 저장온도별로 유의적인 차이가 나타났으며(p<0.01), 상온에서는 6개월 이상, 저온에서는 1년 이상 상품성 유지가 가능하였다.

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