• Title/Summary/Keyword: 지방체

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Antioxidant Enzyme Activities in Tissues of Papilio xuthus during Pupal Stage (호랑나비의 용기 동안 조직 내 항산화 효소의 활성)

  • Shin, Myung-Ja;Kim, Kyoung-Keun;Lim, Jae- Hwan;Jeong, Hyung-Jin;Seo, Eul-Won
    • Journal of Life Science
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    • v.19 no.2
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    • pp.228-233
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    • 2009
  • The purpose of this study is to evaluate the activities of five different antioxidant enzymes in various tissues of Papilio xuthus during pupal stage. Superoxide dismutase (SOD) activity in haemolymph was the highest just after pupation and then decreased gradually until 7 days after pupation but the activity in other tissue was constant during metamorphosis. This result indicates that primary antioxidant process of reactive oxygen species proceed in haemolymph. Catalase (CAT) activity in studied tissues was also the highest just after pupation and its relative activity was also high during pupal stage, suggesting that CAT is the primary enzyme in catalysis of hydrogen peroxide. Glutathion peroxidase (GPX) activity was constant and its relative activity was very low in all tested tissues. Glutathione S-transferase (GST) activity in haemolymph was high at 3 days and 5 days after pupation, and the activity in fat body was the highest at the 1 day after pupation and then decreased gradually for 7 days after pupation. Glutathion reductase (GR) activity in haemolymph and fat body was high at 1 day after pupation, but relatively low GR activity was detected in the rest tissues. Based on these results, GST activity was higher than that of GPX and GR, and it is also believed that GST was more involved in reduction process through lipid peroxidation than GPX.

Effects of insect growth regulators(IGRs) on vitellogenesis in insect (곤충의 난황형성에 대한 곤충성장조절제의 작용)

  • Lee, Hee-Kwon;Lee, Jong-Jin;Kim, Moo-Key;Lee, Hoi-Seon
    • The Korean Journal of Pesticide Science
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    • v.5 no.4
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    • pp.11-19
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    • 2001
  • This review discusses the effects and roles of insect hormones and insect growth regulators (IGRs) on vitellogenesis in adult insects. Insect vitellogenesis is regulated by hormones such as juvenile hormone (JH), ecdysteroids, and neurosecretory hormones (ovaryecdysteroidogenic hormone : OEH) released by neurosecretory cells, diet, and other elements(male specific protein of sperm fluid). In the fat bodies, the vitellogenins are synthesized by the stimulation of JH released by corpus allatum (CA) and ecdysteroids produced by follicle cells with the ovary in most insects. Furthermore, vitellogenins are released into the hemolymph, transported to the ovarioles by carrier protein, and incorporated into oocytes for the developing ovary. Of IGRs, juvenile hormone and its mimics such as methoprene and pyriproxifen appear to have pharmacological effects such as membrane lysis, destruction of salivary grand and midgut epithlial cells, fat body cells, and ovarian tissue, and also anti-juvenile hormone such as precocenes I and II appear to have specific cytotoxicity such as inhibition of corpus allatum and oocytes development. These results suggest that IGRs may be useful as agents for integrated pest management.

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Thermotropic Liquid Crystalline Behavior of Aliphatic Acid Esters of N,O-Hydroxypropyl Chitosans (N,O-히드록시프로필 키토산 지방산 에스터들의 열방성 액정 거동)

  • Kim, Hyo Gap;Jung, Seung Yong;Ma, Yung Dae
    • Polymer(Korea)
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    • v.37 no.3
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    • pp.276-287
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    • 2013
  • Two kinds of N,O-hydroxypropyl chitosans (HPCTOs) with degree of substitution (DS) and molar substitution (MS) ranging from 2.15 to 2.39 and 2.9 to 4.1, respectively, and five kinds of aliphatic acid esters of HPCTOs (HPCTOAms, m=0,2,4,7,9, the number of methylene units in aliphatic substituent) based on the HPCTOs were synthesized, and the thermotropic liquid crystalline properties of the derivatives were investigated. All the derivatives formed enantiotropic cholesteric phases whose optical pitches (${\lambda}_m$'s) increased with increasing temperature. However, the glass and clearing temperatures, the magnitude of ${\lambda}_m$ of the mesophase at the same temperature, and the temperature dependence of ${\lambda}_m$ of the investigated derivatives highly depended on MS and m. The thermotropic mesophase properties of HPCTOAms were significantly different from those reported for the aliphatic acid esters of hydroxypropyl celluloses. The results indicate that the secondary amino group in the C-2 position plays an important role in the thermal stabilization and temperature dependence of ${\lambda}_m$ of the cholesteric mesophase.

Temporal and Spatial Role of Pupal Stage Specific Cuticle Protein in Artogeia rapae (배추흰나비 용기 특이 큐티클 단백질의 시공간적 역할)

  • Shin, Myung-Ja;Park, Jeong-Nam;Seo, Eul-Won
    • Journal of Life Science
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    • v.17 no.2 s.82
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    • pp.223-229
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    • 2007
  • Present study aims to investigate the topical distribution of pupal stage specific cuticle protein and its temporal and spatial role during the wing formation of Artogeia rapae. ArCP27(27 kd cuticle protein) was identified as pupal stage specific cuticle protein in cuticle tissues and has not shown any qualitative differences by local portions of body. ArCP27 maintained constant concentration just after pupal ecdysis to 5-day old pupal stage but thereafter decreased. In fat body, ArCP27 was found in both thoracic and abdominal fat body from the last larval to pupal stage. In wing cuticle, ArCP27 began to find from 5-day old pupal stage. Immunologically ArCP27 in thoracic and abdominal cuticle has the response against the ArCP27 at 5-day old pupa but since then has no response. But the antibody against ArCP27 has reacted to 5- and 7-day old pupal and adult wing protein. $^3H-leucine$ was not incorporated into ArCP27 in 5- and 7-day old thoracic and abdominal cuticle but was incorporated into ArCP27 in 7-day old wing cuticle and adult wing, suggesting that ArCP27 partly participates the wing cuticle formation by the process of digestion and reabsorption of old cuticle.

Molecular Events of Insulin Action Occur at Lipid Raft/Caveolae in Adipocytes (지방세포의 Lipid Raft/Caveolae에서 인슐린의 분자적 작용기전)

  • Bae, Sun-Sik;Yun, Sung-Ji;Kim, Eun-Kyung;Kim, Chi-Dae;Choi, Jang-Hyun;Suh, Pann-Ghill
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.56-63
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    • 2007
  • Insulin stimulates the fusion of intracellular vesicles containing glucose transporter 4 (GLUT4) with plasma membrane in adipocytes and muscle cells. Here we show that adipocyte differentiation results in enhanced insulin sensitivity of glucose uptake. On the other hand, glucose uptake in response to platelet-derived growth factor (PDGF) stimulation was markedly reduced by adipocyte differentiation. Expression level of insulin receptor and caveolin-1 was dramatically increased during adipocyte differentiation. Adipocyte differentiation caused :ilightly enhanced activation of acutely transforming retrovirus AKT8 in rodent T cell lymphoma (Akt) by insulin stimulation. However, activation of Akt by PDGF stimulation was largely reduced. Activation of ERK was not detected in both fibroblasts and adipocytes after stimulation with insulin. PDGF-dependent activation of ERK was reduced by adipocyte differentiation. Insulin-dependent glucose uptake was abrogated by LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor, in both fibroblasts and adipocytes. Also disassembly of caveolae structure by $methyl-\beta-cyclodextrin$ caused impairment of Akt activation and glucose uptake. Finally, insulin receptor, Akt, SH2-domain-containing inositol 5-phosphatase 2 (SHIP2), and regulatory subunit of PI3K are localized at lipid raft domain and the translocation was facilitated upon insulin stimulation. Given these results, we suggest that lipid raft provide proper site for insulin action for glucose uptake.

Comparison of Arylphorin of Antheraea pernyi with Those of Several Lepidopteran Wild Silkmoths by Western Blot Analysis. (작잠(Antheraea pernyi) arylphorin의 항체를 이용한 수종의 나비목 야생 견사곤충들 간의 면역학적 비교)

  • Park, Nam-Sook;Kim, Mi-Ae;Park, Hyun-Chul;Kim, Keun-Ki;Jin, Byung-Rae;Lee, Sang-Mong
    • Journal of Life Science
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    • v.18 no.3
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    • pp.409-413
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    • 2008
  • The occurences of proteins relating to Antheraea pernyi arylphorin in haemolymph, fat body, integument, midgut and silkgland of the wild silkmoths, Antheraea yamamai, Antheraea pernyi, Samia cynthia pryeri and Actias gnoma in the 5th larval instar were investigated by immunoblot analysis using mouse polyclonal antibody against A. pernyi arylphorin as probe. In A. yamamai, A. pernyi, S. cynthia pryeri and A. gnoma, the major immunoreactive antigenic proteins with a molecular weight of 80 KDa against the antisera of the A. pernyi arylphorin were clearly observed in the haemolymph, but in the integument, fat body, midgut and silkgland of the corresponding wild silkmoths the presence of the immunoreactive proteins were very variable. These results suggest that the A. pernyi arylphorin has almost same immunological identity with those of the wild silkmoths, A. yamamai, S. cynthia pryeri and A. gnoma though the distribution of the corresponding antigenic arylphorins is different according to the tissues of the wild silkmoths.

A Case Study on the Influence of the Local Government to the Intangible Cultural Heritage Community - Focused on Oegosan Onggi Village - (지방자치단체가 무형유산 공동체에 미치는 영향 - 외고산 옹기마을 사례를 중심으로 -)

  • Lee, Chae Won
    • Korean Journal of Heritage: History & Science
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    • v.44 no.4
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    • pp.4-17
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    • 2011
  • Oegosan Onggi Village can be characterized by having industrial significance and cultural significance of Onggi. However Onggijang(Onggi maker) worked in poor conditions because the village was in the limited development district. Throughout the new millenium phenomenon of Well-being, the awareness of cultural values has been increased while local government designated Onggi as Local Intangible Cultural Heritage(ICH). In this condition, Ulsan metro city and Ulju county paid attention to Onggi Village as tourist attractions. First, they worked improving condition of village through Cultural Tourist Attraction Plan. Second, they extended their participation in Onggi Festival so the festival growed. Third, they designated Onggijang in Village as Local ICH. These activities were the effort in order to revitalize the village, however the cultural tourist attraction plan was solely centralized into tourist attractions. Thus, the work condition of Onggijang was not considered sufficiently so the work condition had become more inconvenient than the previous situation. In the case of Onggi festival, the outward development has been accomplished in accordance with the growth of festival scale and visitors, while the identity of festival had been weaken so the Onggijang barely attended the Onggi festival. The designation of ICH was also the unilateral decision, Onggijang had been in the conflict relationship between Onggijang concerning the craftsmanship and maintenance of the ICH. Local Government had power on their Intangible Cultural Heritage(ICH). Therefore we need to seek for role and functions as community on ICH.

국회 토론회 지상중계 - 위기의 학교우유급식 '최저가 입찰제' 이대로 좋은가

  • 한국낙농육우협회
    • 월간낙농육우
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    • v.36 no.11
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    • pp.122-132
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    • 2016
  • 2015년 감사원에서 지방교육청 감사 이후 교육부에 학교우유급식 공급업체 선정시 입찰을 실시할 것을 권고하면서 올해부터 학교우유급식 최저가 입찰제가 본격 확대됐다. 최저가 입찰제 시행 1년에 이른 지금, 유업체간 출혈경쟁으로 납품을 포기하는 사례가 발생하고, 터무니없이 낮은 가격은 급식우유의 품질을 의심하게 만들었으며, 도농간 우유급식 공급가격 격차가 발생, 칼슘 섭취 사각지대에 놓인 학생들이 오히려 많아지게 됐다. 본회 낙농정책연구소에서는 홍문표 국회의원, 김성원 국회의원과 함께 지난 10월 26일 국회의원회관 대회의실에서 토론회 "위기의 학교우유급식 '최저가 입찰제' 이대로 좋은가"를 개최하여 최저가 입찰제에서 야기된 부작용과 해결방안을 찾아보는 자리를 마련했다. 열띤 국회 토론회 현장을 지상중계한다.

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사업탐방-전라북도

  • Lee, Gyeong-Ok
    • 건강소식
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    • v.32 no.7
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    • pp.32-33
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    • 2008
  • 한반도 서남부에 위치한 전라북도는 산과 들, 바다가 어우러진 아름다운 지역이다. 동쪽은 높고, 서쪽은 낮은 계단식 지형을 이루고 있는 쌀의 주산지로 한국농업의 상징인 곳이다. 전라북도의 자치행정을 관장하는 지방행정기관 전라북도 도청은 전주시 완산구 효자동에 자리 잡고 있다. 복지 공동체 구현, 선진농업 육성, 지역경제의 발전, 문화관광 특성화 등의 사업을 추진 중인 전라북도는 지역민들이 쉽게 문화나 여가를 즐길 수 있도록 편의시설을 두루 갖춘 곳이기도 하다. 특히 새만금 사업과 태권도 공원을 추진 중에 있는 등 지역발전에 앞장서고 있다. 그리고 무엇보다 전라북도는 한국건강관리협회 전북지부와 함께 지역주민들에게 더 나은 삶을 제공하고 건강한 신체와 건강한 정신을 추구하고자 건강검진 사업을 활발히 진행할 예정에 있다.

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충청남도 자원봉사활동 활성화 방안

  • Ko, Seung-Hee
    • Proceedings of the Korea Contents Association Conference
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    • 2007.11a
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    • pp.743-746
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    • 2007
  • 자원봉사활동은 지역사회 공동체와 연대의식 강화 등 지역사회를 지탱해 주는 주요한 토대로서 지방정부의 입장에서는 지역주민의 직 간접자원을 활용할 수 있는 계기되고 개인적 입장에서는 지역사회에 기여를 통한 만족감 제고라는 상호이익이 보장되는 중요한 활동이다. 따라서 자원봉사활성화를 위해 과감한 재정 및 제도적 뒷받침을 마련해 주는 동시에 민간중심의 활동이 될 수 있도록 자율성과 창의성을 최대한 보장해 주어야 한다.

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