• Title/Summary/Keyword: 지방산

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Tylosin Production by Mutant Resistant to Oleic Acid (Oleic Acid 내성균주로부터 Tylosin 생산)

  • Choi, Du-Bok;Choi, On-You;Moon, Ok-Ran;Yoon, Mi-Ran;Ji, Sung-Nam;Shin, Dae-Yewn
    • Journal of Environmental Health Sciences
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    • v.31 no.5 s.86
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    • pp.360-364
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    • 2005
  • When rapeseed oil as the carbon source was used for tylosin production from Streptomyces fradiae TP-1239 was very sensitive to oleic acid. Cell growth was restrained by adding 0.8 g/l of oleic acid to the culture broth. Mutant strain TM-224-1 resistant to 1.2 g/l of oleic acid was obtained by screening in solid and liquid media containing oleic acid. The uptake rate of oleic acid by TM-224-1 was approximately 3.8 fold higher than the parent strain. For comparing the TM-224-1 and the parent strain, batch cultures were carried out in a jar fermentor. Cell growth of TM-224-1 strain was higher than the parent strain after two days of culturing. However, after four days of culturing, it was similar to that of the parent strain. The amount of rapeseed oil consumed by TM-224-1 and the parent strain were 60.5 and 78.2 g/l, respectively. The production and yield of tylosin was aproximately 2.0 and 3.2 fold higher than the parent strain, respectively. From these results, it was concluded that this mutant, which was resistant to oleic acid, has improved tylosin production.

Physicochemical Properties of Jatjook as Influenced by Various Levels of Pinenut (잣의 첨가량에 따른 잣죽의 특성)

  • 이승현;장명숙
    • Korean journal of food and cookery science
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    • v.10 no.2
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    • pp.99-103
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    • 1994
  • The purpose of this study was to investigate the sensory and physicochemical properties of jatjook(rice gruel cooked with pinenut) containing various levels of pinenut. For this purpose, the various methods and recipe of Jatjook appeared in the literatures were evaluated to select the most preferable Jatjook. Chemical composition of Jatjook containing various levels of pinenut were 69.75∼83.04% of moisture, 2.25∼4.85% of crude protein, 1.77∼ 13.09% of crude fat, and 0.13∼0.51% of ash. C18-2,(linoleic acid) was the highest in the fatty acid contents of jatjook. Vitamin I contents of jatjook were 0.50∼3.50mg/100g. By the color difference meter, the values of L(lightness) and b(yellowness) were increased by increasing the amount of pinenuts, whereas the value of a(redness) showed negative value by increasing the amount of pinenut. Viscosity of Jatjook was decreased by increasing the amount of pinenut. As a result of the sensory evaluation fo. Jatjook made with various levels of pinenut, Jatjook having mixture ratio of 1 rice, 1 pinenut, and 5 water was the most preferable.

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Effects of Dietary Onion Supplementation of on the Physicochemical Properties of Duck Meat (양파의 급여가 오리고기의 이화학적 특성에 미치는 영향)

  • 송영민;진상근;김일석;조용조;김회윤;하경희;남기윤
    • Food Science of Animal Resources
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    • v.24 no.1
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    • pp.66-72
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    • 2004
  • The study was conducted to investigate the effect of dietary onion supplementation on physicochemical properties of duck meat. The ducks were raised for 38 days(1,500 g of live weight) in rice paddle and slaughtered. Samples including breast and leg were stored at 4$^{\circ}C$ for 12hrs and then used as experimental materials. The ducks were allotted into 3 treatments(C-0%, T1-3%, T2-6%) according to dietary supplementation of onion. The ducks meat were stored at 4${\pm}$1$^{\circ}C$ for 12hrs. In proximate analyses, the moisture, crude protein and crude ash of breast muscle were significantly increased(p<0.05) with increasing onion percentages but crude fat content was significantly decreased(p<0.05). The moisture and crude protein of leg muscles were significantly increased(p<0.05) with increasing onion percentages but crude fat and crude ash were significantly decreased(p<0.05). The pH of breast muscle was significantly decreased(p<0.05) with increasing onion percentages, the pH of leg muscle was be highest in T1(p<0.05). Water holding capacity(WHC) significantly increased(p<0.05) with increasing onion percentage in both muscles. Hunter L* was significantly decreased with increasing onion percentage in breast muscles while Hunter b* significantly increased(p<0.05). In fatty acids composition of duck meat, mono-unsaturated fatty acid(MUFA) significantly decreased(p<0.05) while poly-unsaturated fatty acid(PUSFA) and EFA(essential fatty acid) were significantly high(p<0.05) with increasing onion percentage.

Study on the Quality Improvement of Sardine Surimi (정어리 냉동고기풀의 품질개선에 관한 연구)

  • Oh, Kwang-Soo;Moon, Soo-Kyung;Lee, Eung-Ho;Kim, Bok-Gyu
    • Korean Journal of Food Science and Technology
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    • v.25 no.4
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    • pp.327-333
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    • 1993
  • The processing conditions and quality of sardine surimi were examined: Raw sardine meat was separated, washed in 0.2% $NaHCO_3$ and 0.15% NaCl solution, and then dewatered by centrifuge. The dewatered sardine meat was chopped, mixed with 20% emulsion curd (soybean protein : water : refined sardine oil=1:5:2.6), 4% sorbitol, 4% sucrose, 0.2% polyphosphate and 0.1% sodium erythorbate by stone mortar. The mixed sardine meat was frozen with contact freezer, packed in carton box and then stored at $-25{\pm}2^{\circ}C$. The moisture, crude protein and lipid contents of the sardine surimi product was 73.3%, 15.0% and 6.9%, respectively. Fatty acid composition of product consisted of 28.8% of saturates, 24.3% of monoenes and 47.7% of polyenes and the major fatty acids were 16:0, 20:5, 18:1, 22:6 and 16:1. The results of changes in POV, TBA value, fatty acids, texture and sensory score of products during frozen storage showed that lipid oxidation and freeze denaturation of product could be retarded, and flavor enhanced by addition 20% emulsion curd and 0.1% sodium erythorbate. In an attempt to apply sardine surimi in producing surimi-based product, it was concluded that pollack surimi could be substituted with sardine surimi up to 40% without showing any significant changes in texture and taste of surimi-based product.

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Shelf-life and Microbiological Study of Sansung Takju (향토주인 산성막걸리의 미생물학적 고찰과 저장성에 관한 연구)

  • Yang, Ji-Young;Lee, Ke-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.4
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    • pp.779-785
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    • 1996
  • Sansung takju, the Korean traditional liquor at a local area of Sansung in Pusan, has been widely drunken due to its tradition in this area. The studies on microorganisms of kokja, fermentation process and shelf-life of takju were carried out. The most abundant microorganism identified from a commercial Sansung kokja was Aspergillus, Mucor, Rhizopus and Penicillium were also identified. In case of a home-made Sansung kokja, Mucor was the most abundant one. Aspergillus, Rhizopus and Absidia were also identified in it. Saccharomyces, Micrococcus and Bacillus were identified in both kokja's but acid production bacteria were not found. Ethanol fermentation was carried out at $25^{\circ}C$ and $30^{\circ}C$ using each kokja. The rate of ethanol production was faster at $30^{\circ}C$ than at $25^{\circ}C$, while higher viable yeast count and final ethanol content were obtained at $25^{\circ}C$ than at $30^{\circ}C$. The ethanol contents of the mashes using a commercial Sansung kokja and a home-made Sansung kokja after 14 days at $25^{\circ}C$ were 11.0% and 12.4%, respectively. The shelf-life of takju was affected more by ethanol content in the product than by storage temperature. The product stored at $-15^{\circ}C$ did not change significantly in acidity but tasted watery due to thawing. In case of Sansung takju containing 6%ethanol, level of acidity increased and pellicle was formed on the surface of the product during storage at $30^{\circ}C$. In case of Sansung takju containing 9% or 12% ethanol, no significant changes in acidity and appearance were observed for 14 days at $30^{\circ}C$.

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Effects of a Caragana sinica Water Extract on Lipid and Glucose Metabolism in Ovariectomized Rats (골담초 열수 추출물의 갱년기 이후 지질 및 당질 대사 개선 효능)

  • Lee, Sang Chul;Chung, Soo Im;Kang, Mi Young
    • Korean Journal of Plant Resources
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    • v.29 no.5
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    • pp.532-538
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    • 2016
  • Menopause is characterized by a decrease in estrogen production by the ovaries. Furthermore, the risk of developing chronic diseases including cardiovascular disease, obesity, and diabetes increases in menopausal women. In this study, we evaluated the ability of Caragana sinica (CS) to affect lipid and glucose metabolism in an ovariectomized Sprague-Dawley rat model of induced menopause. The animals were divwided randomly into three groups: sham-operated rats (SHAM), ovariectomized rats (OVX), and ovariectomized rats treated with 1% water extract of CS (OVX-CS). The OVX-CS treated mice showed a significant decrease in body weight, adipose tissue, triglyceride, and total cholesterol. Improved lipid and glucose profiles were also observed, and were attributed to suppression of hepatic lipogenesis and adipokine production and regulation of glucose-regulating enzyme activity. Therefore, these findings indicate a potential use for CS as a functional food for menopause-induced hyperlipidemia and hyperglycemia.

Effect of Dietary n-3 Fatty Acids and Fat Unsaturation on Plasma Lipids and Lipoproteins in Rats (식이의 n-3 지방산과 지방의 불포화도가 혈장지질 조성에 미치는 영향)

  • 박현서
    • Journal of Nutrition and Health
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    • v.25 no.7
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    • pp.555-568
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    • 1992
  • This study was to compare the effects of dietary n-6 and n-3 fatty acids and fat unsaturation on plasma lipids and chemical composition of VLDL and LDL fraction and lipogenic enzymes activity in rat liver under the conditions providing 1) a similar amount of n-6, n-3 fatty acids(LA, ALA, EPA+DHA) in diets and 2) the various degree of fat unsaturation. Male Sprague-Dawley rats weighing 420g were treated for 6-n with six experimental diets providing 25% of energy as fat and which were different only in fatty acid composition. The fats used for a source of each fatty acid were beet tallow for saturated fatty acid corn oil for n-6 linoleic acid(LA) perilla oil for n-3 $\alpha$-linolenic acid(ALA) and fish oil n-3 eicosapentaenoic acid (EPA) and n-3 docosahexaenoic acid(DHA). Plasma cholesterol level was increased by corn oil to compare with beef tallow but was decreased by perilla oil or fish oil. Plasma TG level was significantly decreased by perilla oil or fish oil. Fish oil significantly reduced the level of HDL-Chol and the proportion of Chol in LDL fraction and that of TG in vVLDL fraction. Overall there was a singificant negative correlation between the level of each plasma lipid(Chol TG, VLDL-TG, LDL-C) and the degree of fat unsaturation. However this rerlationship is not always true when compared the hypolipidemic effect of each fatty acid at a similar level of fat unsaturation. There was a trend such taht glucose 6-P dehydrogenase 6-phosphogluconate dehydrogenase and malic enzyme activites were reduced by n-3 fatty acids. Perilla oil significantly increased the incorporation of c20:5 and c22:5 into liver tissue and fish oil suignificantly increased the incorporation of c20:5, c22:6 into liver tissue and the effect of long chain n-3 fatty acid incorporation was greater by fish oil. therefore the hypotriglyceridemic effect of n-3 fatty acid could be resulted from the interference of hepatic lipogenesis by long-chain n-3 fatty acids and the reduced proportion of TG in VLDL fraction and its effect was greater by n-3 EPA+DHA than n-3 ALA even though plasma Chol and TG levels were also influenced by the degree of dietary fat unsaturation.

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The Composition of Candida guilliermondii Grown in Soybean Whey (Soybean Whey에 배양(培養)한 Candida guilliermondii의 균체조성(菌體組成))

  • Lee, Kang-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.11 no.1
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    • pp.21-24
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    • 1982
  • A strain of yeast (Candida guilliermondii KFCC 35120) was cultivated by using the soybean whey with supplement of blackstrap molasses (4%) and ammonium acetate (0.5%). By the cultivation continued for 48 hours, 8.1g of dry cell was produced. The cell was consisted of carbohydrate 39.3%, protein 40.5%, lipid 3.9%, nucleic acid 9% and ashes 6%. The protein was analyzed to contain 18% of glutamic acid and 10% of lysine. Other amino acids showed a content near that of FAO reference protein except Methionine. Total lipid content was varied by nutritional condition especially by the kind of carbon source, however it showed a content 1.7 to 2.5 times higher than that of Saccaromyccs cerevisiae. The lipid contain 48% of oleic acid, 17% linoleic acid and 4% linolenic acid. The content of linoleic and lonolenic acid was higher than that of S.cerevisiae by 9 and 24 times, respectively.

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Effects of Bamboo Charcoal and Bamboo Leaf Supplementation on Performance and Meat Quality in Chickens (대나무 숯과 대나무 잎의 급여가 육계의 생산성과 육질에 미치는 영향)

  • Kim, Sung-Hwan;Lee, In-Chul;Kang, Sung-Su;Moon, Chang-Jong;Kim, Sung-Ho;Shin, Dong-Ho;Kim, Hyoung-Chin;Yoo, Jin-Cheol;Kim, Jong-Choon
    • Journal of Life Science
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    • v.21 no.6
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    • pp.805-810
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    • 2011
  • The present study was conducted to investigate the effects of dietary supplementation with bamboo charcoal (BC) and bamboo leaf (BL) on growth performance, feed intake, feed conversion efficiency, and meat quality in broiler chickens. Broiler chickens were fed for 30 days with a diet containing 0.5% bamboo charcoal or bamboo leaf. The results showed that the mortality rate during the study period was slightly lower in the BC and BL groups than the control group, while terminal weight and weight gain were significantly higher in the BC and BL groups than the control group. Dietary supplementation with BC or BL also improved feed conversion rate compared to chickens in the control group. Shear force was significantly lower in the BC and BL groups than that of the control group. The fat content of chickens fed with BC tended to decrease, while the ratio of unsaturated fat acid of chickens fed with BC or BL tended to increase, although without a statistically significant difference. Sensory evaluation revealed that overall acceptability was slightly higher in the BC and BL groups than the control group. There was no statistically significant change in the hematology and serum biochemistry parameters, compared with the control group in any group tested. The results of this experiment indicated that dietary supplementation with BC and BL may improve growth performance, feed conversion efficiency, and meat quality in broiler chickens.

The Effect of Freezing Rates on the Physico-Chemical Changes of Beef during Frozen Storage at $-20^{\circ}C$ (동결속도에 따른 쇠고기의 냉동저장중 이화학적 변화)

  • Kim, Young-Ho;Yang, Seung-Yong;Lee, Moo-Ha
    • Korean Journal of Food Science and Technology
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    • v.20 no.3
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    • pp.447-452
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    • 1988
  • In order to study effect of freezing rates on the quality changes such as pH, TBA value, free fatty acids and protein extractability, cylindrical chopped beef logs with 10cm of diameter and 10cm of height were frozen at three freezing rates(0.97cm/hr, 2.05cm/hr, 3.71cm/hr)using air blast freezer. Physicochemical changes of frozen meat were investigated during forzen storage at $-20^{\circ}C$ for 16weeks. Results on pH change showed $0.1{\sim}0.2unit$ increase at the 16th week of the frozen storage and the change was smaller with the increasing freezing rates. Free fatty acids content and TBA value also were increased during forzen storage, but they were minimal at 3.71cm/hr freezing rate. Correlation coefficient between TBA value and free fatty acids content were highly significant(r=0.804). After 16weeks of storage, extractibilities of salt soluble protein were decreased by 17.7%, 6.1% and 1.6% at freezing rates of 0.97, 2.05 and 3.71cm/hr, respectively. On the other hand, extractabilities of water soluble protein were decreased by 26.0%, 21.2% and 18.5%, respectively. The effect of freezing rates on the protein extractability appeared to be greater in salt soluble protein than in water soluble protein, but freezing denaturation was more rapid in water soluble protein.

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