• Proceedings of the Korean Society of Life Science Conference
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• 2001.02a
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• pp.56-56
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• 2001

• Proceedings of the Korean Society of Life Science Conference
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• 2000.09a
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• pp.48-48
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• 2000

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.515-518
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• 2004

Anemarrhena asphodeloides, a medicinal plant, has chromosome number of 2n=2x=22. To characterize the somatic metaphase chromosomes, physical mapping of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) was applied. Two pairs of 45S rDNA loci were detected on the terminal regions of the short arm of chromosomes 1 and 3. A pair of 5S rDNA signal was observed on the short arm of chromosome 3. 5S rDNA site seemed to be the same locus as one of the 45S rDNA site. McFISH was very useful tool for the localization and identification of rDNAs on the metaphase chromosomes in A. asphodeloides.

• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.31-38
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• 2015

The rhizomes of Anemarrhena asphodeloide was extracted with 100% ethanol and concentrated subfractions were separated with medium pressure liquid chromatography-based activity profiling. One compound was isolated from the subfraction 10 through the repeated preparative high performance liquid chromatography (prep-HPLC). According to physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compound was determined as nyasol (1). Nyasol was exhibited potent inhibitory activity for NO ($IC_{50}:12.5{\mu}g/mL$), tyrosinase ($IC_{50}:12.5{\mu}g/mL$), melanin contents ($IC_{50}:12.5{\mu}g/mL$), thymus and activation-regulated chemokine (TARC) production ($IC_{50}:6.25{\mu}g/mL$). As a result, nyasol has an excellent inflammation-dependent anti-whitening and TARC production activity. It could be used to a large range of functional cosmetics.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.109-116
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• 2000

This study was carried out to screen herbs among herbal plants showing antimicrobial activity against some food spoilage and pathogenic microorganisms. One hundred fifteen kinds of herbal plants were extracted by 70% ethanol, and then they have been screened for antimicroorganisms. Six herbal plants such as Salviae radix, Dryopteris rhizoma, Terminaliae fructus, Araliae radix, Psoraleae fructus and Schisandrae fructus showed strong antimicrobial activities against Bacillus subtilis. Antimicrobial activities were showed in Anemarrhena radix and Dryopteris rhizoma on Candida albicans, and in Anemarrhenae radix, Dryopteris rhizoma and Polygalae radix on Schizosaccharomyces sp. It was revealed that eight herbal plants such as Dryopteris rhizoma, Salviae radix, Sappan ligunum, Sinomeniae radix, Schisandrae fructus, Rhui fructus, Sophorae radix and Inulae radix also showed antimicrobial activities on Streptococcus mutans. In addition, Anemarrhena radix, Curcuma tuber, Inulae radix, Polygonum radix, Sanguisorbae radix, Scutellariae radix and Terminaliae fructus and showed antimicrobial activities on Trichophyton mentagrophytes. Four kinds of herbal plants such as Dropteris rhizoma, Salviae radix, Terminaliae fructus and Scutellariae radix which showed broad antimicrobial spectrums were mixed by 1 : 1 ratio with the other herbal paints showing relatively strong microbial activities such as Terminaliae fructus, Sinomeniae radix and Scutellariae radix etc. The extracts of mixed herbal paints showed higher antimicrobial activities than those of single herbal plant.

• Food Science and Preservation
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• v.21 no.3
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• pp.421-426
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• 2014

In this study, the antioxidant and pancreatic lipase inhibitory activities of aqueous methanolic (70% methanol) extract from the roots of Anemarrhena asphodeloides were investigated. The extracts of four solvent fractions (the n-hexane layer, EtOAc layer, n-BuOH layer, and $H_2O$ layer) of the 70% methanol extract were also investigated. Furthermore, the total phenolic content was quantified using a spectrophotometric method. All the tested samples showed dose-dependent radical scavenging and pancreatic lipase inhibitory activities. In particular, the pancreatic lipase inhibitory activity of the ethyl acetate soluble portion (the EtOAc layer) from the rhizomes of the A. asphodeloides was higher than that of the other solvent-soluble portions. The antioxidant property of the extracts was evaluated using radical scavenging assays with DPPH and $ABTS^+$ radicals. 1000 mg/ml of the n-BuOH layer extract showed 91.2% DPPH radical scavenging activity. The EtOAc layer extract and the n-BuOH layer extract showed $IC_{50}=20.5{\pm}1.7mg/ml$ and $IC_{50}=50.5{\pm}0.7mg/ml$ $ABTS^+$ radical scavenging activities, respectively. The anti-obesity efficacy of the A. asphodeloides extract was tested via porcine pancreatic lipase assay. A pancreatic lipase inhibitory activity ($IC_{50}$) of $31.3{\pm}0.1mg/ml$ was obtained from the EtOAc layer extract. These results suggest that A. asphodeloides can be considered a new potential source of natural antioxidant and anti-obesity agents.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.347-355
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• 2006

Possible nematicidal effects of plant extracts of 25 species uninfected by M. hapla were observed at the 5 times dilutions in all treatments and at the 10 times dilutions in Anemarrhena asphodeloides, Acorus calamus, Achyranthes japonica, Agrimonia pilosa, Dianthus chinensis, Geum aleppicum, Houttuynia cordate, Rudbeckia bicolor, Ricinus communis, Scrophularia buergeriana, Sesamum iindicum, Sedum kamtschaticum, and Sanguisorba officinalis. The 13 species plant extracts of 5 times dilutions were evaluated for the suppression effects on reducing densities of M. hapla by treating to C. lanceolata sown and transplanted later in pots. All the plant extracts showed suppressive effects on M. hapla except for.A. pilosa. The suppressive effects of A. asphodeloides, A. japonica, A. calamus, D. chinensis, R. communis, and S. buergeriana were over 80%. When the selected plants had been incorporated into the soil before C. lanceolata was sown, the numbers of root galls, egg sacs and $J_{2}$ appeared lower in the treatment of 12 plant species than in control except for S. indicum. But the suppressive effects were lower than the effects of selected plants being cultivated simultaneously in the field. A. calamus and A. japonica exhibited over 70% suppressive effects, among the tested plants.

• Journal of the Korean Vacuum Society
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• v.3 no.1
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• pp.1-7
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• 1994

전자방출에서 에너지 교환과 역온도를 새롭게 계산하였다. 전자방출에서 대체전자들에 유용한 빈상태들을 만드는 요소로 터널링 효과를 이 계산에서 고려하였다. 그것은 방출부분에서 일어나는 대체 관정을 설명하기 위해서는 빈상태를 만드는데 터널링 기여가 필수적이기 때문이다. 이렇게 계산하여 온 도와 전장의 함수로 얻어진전자당 에너지 교환은 실험결과와 잘 일치하였다. 또한 전장의 함수로 얻어 진 역온도도 실험치와 만족할 만한 일치를 보여주었다. 이 결과는 대체전자들의 평균에너지에 대한 노 팅햄-플레밍과 헨더슨의 논쟁에서 후자의 견해를 지지하는 것이다.

• Communications for Statistical Applications and Methods
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• v.3 no.1
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• pp.121-130
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• 1996

본 논문에서는 사회적 통념상 또는 개인적으로 매우 민감한 사안에 대한 조사시 무응답이나 거짓응답으로 인한 비표본오차를 줄여서 추정의 신뢰성을 높일 수 있도록 고안된 확률화응답기법을 이용하여 이지모집단내의 민감집단의 비율을 추정할 때 신분보호 정도가 같게 되는 조건하에서 추정의 효율을 높일 수 있는 의사 2단계 확률화응답기법을 제안하고 여러 형태의 확률화응답기법들과의 효율성 비교를 통하여 제안된 기법이 효율적일 수 있는 조건을 찾아보았다.

• Proceedings of the Korean Society of Life Science Conference
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• 2003.05a
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• pp.68-68
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• 2003