• Food Engineering Progress
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• v.15 no.4
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• pp.370-375
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• 2011

In this study the polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens in fresh originally grown vegetables. A universal primer (341GCf/534r) was selected for its ability to amplify the V3 region of 16S-rRNA genes in their target pathogens (Salmonella typhimurium, Pseudomonas fluorescens, Bacillus cereus, Listeria monoytogenes, Staphyloocus aureus, E. coli). The 194 bp fragments in PCR were successfully duplicated as expected. The amplified fragments of the same size from six different pathogens also showed good separation upon DGGE. The detection limit of PCR-DGGE for six pathogens in fresh-cut lettuces were over $10^{5}$ CFU/g when sampled by stomaching. However, when the sampling method was changed from stomaching to shaking, the detection limit of six pathogens in organic vegetables was shown to increase by over $10^{1}$ CFU/g, but only those of B. cereus were over $10^{3}$ CFU/g. Therefore, PCR-DGGE was shown to be a reliable method for the detection of pathogens in fresh-cut vegetables.

• The Journal of the Korea institute of electronic communication sciences
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• v.19 no.2
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• pp.355-360
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• 2024

In this paper, in implementing a 4-coil resonant wireless power transmission system, we studied the discharge phenomenon that occurs at the end of the transmitting resonance coil. Resonant wireless power transmission consists of a power supply coil, a transmitting resonance coil, a power receiving resonance coil, and a load coil. The transmitting resonance coil serves to amplify the magnetic field generated from the power supply coil and transmits it to the front receiving resonance coil. When a high current flows through the power supply coil in order to transmit large power, a high voltage is induced at the end of the transmitting resonance coil. It causes line-to-line discharge. Line-to-line discharge phenomenon damages the transmitter case and renders the transmitter unusable. Therefore, in order to eliminate this line-to-line discharge phenomenon, the voltage induced in the transmitting resonance coil that causes line-to-line discharge was analyzed and a solution was proposed.

• Clinical and Experimental Pediatrics
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• v.45 no.3
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• pp.354-361
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• 2002

Purpose : Selective introduction of genes conferring chemosensitivity into proliferating tumor cells may be used to treat cancer. We first investigated the bystander effect of retrovirus-mediated gene transfer of herpes simplex virus thymidine kinase(HSV-TK) gene to murine neuroblstoma cell line(neuro-2a) in vitro and in vivo. Second, we examined the mechanism and its enhancement of the bystander effect in murine neuroblastoma. Methods : To investigate the bystander effect, we studied tumor growth and survival time after HSV-TK/ganciclovir(GCV) treatment in a syngenic A/J mouse neuroblastoma model by mixing various ratios of HSV-TK-expressing neuro-2a cells with wild type neuro-2a cells followed by GCV treatment. To investigate the mechanism of the bystander effect in murine neuroblastoma, immunohistochemistry using connexin 43, CD4 and CD8-specific monoclonal antibodies was analyzed. We studied whether IL-2-secreting neuro-2a cells(neuro-2a/IL-2) would potentiate the bystander effect. Results : A strong bystander effect was observed in vitro and in vivo. The bystander effect in murine neuroblastoma was dependent on the immune response rather than connexin-mediated gap junction. Neuro-2a/IL-2 treatment enhanced the bystander effect in the HSV-TK/GCV system in murine neuroblastoma model. Conclusion : We conclude that the bystander effect in murine neuroblastoma depends on immune response and is enhanced by neuro-2a/IL-2.

• Journal of the Korean Geotechnical Society
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• v.36 no.2
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• pp.5-15
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• 2020

The liquefaction phenomenon that occurred during the Pohang earthquake (M_L=5.4) brought new awareness to the people about the risk of liquefaction caused by the earthquake. Liquefaction hazard maps with 2 km grid made in 2014 used more than 100,000 borehole data for the whole country, and regions without soil investigation data were produced using interpolation. In the mapping of macro liquefaction hazard for the whole country, the site amplification effect and the ground water level 0 m were considered. Recently, the Ministry of Public Administration and Security (2018) published a new site classification method and amplification coefficient of the common standard for seismic design. Therefore, it is necessary to rewrite the liquefaction hazard map reflecting the revised amplification coefficient. In this study, the results of site classification according to the average shear wave velocity in soils before and after revision were compared in the whole country. Also, liquefaction assessment results were compared in Gangseo-gu, Busan. At this time, two ground accelerations corresponding to the 500 and 1,000 years of return period and two ground water table, 5 m for the average condition and 0 m the extreme condition were applied. In the drawing of liquefaction hazard map, a 500 m grid was applied to secure a resolution higher than the previous 2 km grid. As a result, the ground conditions that were classified as S_C and S_D grounds based on the existing site classification standard were reclassified as S₂, S₃, and S₄ through the revised site classification standard. Also, the result of the Liquefaction assessments with a return period of 500 years and 1,000 years resulted in a relatively overestimation of the LPI applied with the ground amplification factor before revision. And the results of this study have a great influence on the liquefaction assessment, which is the basis of the creation of the regional liquefaction hazard map using the amplification factor.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.11
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• pp.56-63
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• 2012

In this paper, adaptive unsharp masking using bilateral filter, edge-preserving smoothing filter is proposed to reduce the overshoot and jagging artifact in sharpening images. Previous image enhancement methods including unsharp masking(UM) can emphasize high-frequency details strongly, but often cause several artifacts such as overshooting, noise, jagging and so on. Proposed image enhancement method preserves edges well because of using bilateral filter and sensitively controls a weight according to edge's directions. Therefore, it enhances sharpness and effectively reduces overshoot and jagging artifacts. Simulation results comparing output of previous AUM with proposed method show that proposed algorithm makes images properly enhanced, and we know that overshoot and jagging artifacts are many reduced.

• Journal of Digital Contents Society
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• v.12 no.3
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• pp.371-382
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• 2011

Reading and writing in the past make noble significance in 'narration', in other words, in 'the creation of narration', through the process of 'recording'; but in the modern times, it engenders controversies over narration and linearity. In other words, reading/writing in digital era, is devalued as the simple arrangement of test or the connection of vast information without narration nor linearity. However, the reading/writing through text and hypertext reading is not the phenomenon which should be criticized because of the lack of narration or linearity-not only the lack of narration and linearity -, a process of social and cultural transition; it should be revalued as a result. The change of reading and writing methods will inevitably accompany the layers, status, significance and value of the contents; thus it makes more sense, when the reading and writing methods in digital contents are approached as new pop culture phenomenon. This is the "The prose of everyday life", based on pop culture and "The society of Conversation", based on communication; proposed 'Digitelling' ; this is the reason why we should pay attention to the digital contents, created infinitely by the citizens of the world, as new mass-culture phenomenon.

• Journal of the Korean Electrochemical Society
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• v.16 no.2
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• pp.70-73
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• 2013

Iridium oxide is well known as an electrocatalyst for the water oxidation. Recently, Dr. Bard's group observed the electrocatalytic behavior of individual nanoparticle of Iridium oxide using the electrochemical amplification method by detecting the single nanoparticle collisions at the ultramicroelectrode (UME). However, the electrocatalytic current is decayed as a function of time. In this study, we investigated that the reason of electrocatalytic current decay of water oxidation at Iridium oxide nanoparticles. We identified it is due to the bubble overpotential because the cyclic current decay and recovery were synchronized to the oxygen bubble growth and coming away from an Iridium disk electrode.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.1914-1918
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• 2006

Recently, the importance of ocean becomes more serious. Thus, we need to construct port structures and instruments safely. Especially, we should understand the diffraction phenomenon of wave in order to construct breakwaters. To simulate diffraction of wave, parabolic mild slope equations are solved using FDM. A breakwater with an open part and an half infinite breakwater are selected for simulation. Diffraction of wave are simulated in the condition of wave angles of attack of $0^{\circ},\;30^{\circ}\;and\l;60^{\circ}$. Diffraction Coefficient and 1)Ampplitude are shown in graphics and compared with results of Penny & Price and Memos.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.7 no.1
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• pp.46-56
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• 1995

Three Pressure type wave gauges were installed for about 10 days for the analysis of long wave agitations in Mukho Harbor. Helmholtz and second resonant periods of seiche in Mukho Harbor are shown to be approximately 10.0-14.3 and 3.3 minutes from the spectral analysis of measured wave data. Amplification ratio at Helmholtz period reaches about 6.8 and the wave amplitudes in the harbor were in the range of 5-10 cm during the measurement period. Helmholtz and second resonant periods of seiche in Mukho Harbor agree very well with those computed using Jeong dt al. (1993b)'s model. The model gives rise to the first and third resonant peaks at 7.5 and 1.9 minutes, respectively.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.27.1-27.1
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• 2015

천문학적 유체는 대부분 자기장을 가지고 있으며 난류상태에 있다고 믿어진다. 본 발표에서는 다양한 환경에서 존재하는 자기유체역학적(MHD) 난류를 소개하고자 한다. 첫째, 가장 간단한 경우로 비압축성 유체에서 발생하는 MHD 난류를 살펴보고자 한다. 이 경우, 평균자기장의 세기가 약한 경우와 강한 경우로 나누어 볼 수가 있는데, 평균자기장의 세기가 아주 약한 경우 난류에 의한 자기장의 증폭 현상이 특히 중요하다. 평균자기장의 세기가 강한 경우는 난류의 스펙트럼과 구조가 큰 관심사가 되고 있다. 둘째, 작은 스케일 난류와 초음속 압축성 난류를 간단히 소개하고자 한다. 작은 스케일(이온의 자이로 반경 부근) 난류는 아직 연구가 미진한 분야 중 하나이고 초음속 압축성 난류는 해석적 연구가 어렵기 때문에 연구의 많은 부분을 수치계산에 의존하고 있다. 마지막으로, MHD 난류에 대한 지식이 어떻게 관측에 응용될 수 있는지 간단한 예를 들고자 한다.