• Title/Summary/Keyword: 증식과 분화

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The Rhizome Growth and Shoot Induction Influenced by Ethylene in Cymbidium niveo-maginatum (옥화란(Cymbidium niveo-maginatum) Rhizome의 생장 및 유식물체 분화에 미치는 Ethylene의 영향)

  • 민병훈;정해준;이은경;황혜연;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.6
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    • pp.515-518
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    • 1998
  • The effect of ethylene on the proliferation of rhizomes and plant regeneration were investigated from rhizome segment culture of Cymbidium niveo-marginatum. Ethylene levels in the rhizome culture vessels were reached a maximum after 8 days of culture; total amount of ethylene evolution was much on the initiation of shoot induction than of rhizome proliferation. The treatment with ethephon on rhizomes was inhibited in the proliferation of rhizome and the growth of shoot length; however, the treatment was effective on shoot induction from rhizomes. Aminoethoxyvinylglycine(AVG) 1mg/L was effective on the proliferation of rhizomes and shoot induction from them; however, the proliferation of them was inhibited, and the growth of shoot length was significantly promoted at the concentration of 10mg/L AVG. The presence of $\textrm{AgNO}_{3}$ inhibited in the proliferation of rhizomes and shoot induction from them.

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Effects of Kohlrabi (Brassica oleracea var. Gongylodes) on Proliferation and Differentiation of Pig Preadipocytes and 3T3-L1 Cells (콜라비가 돼지 지방전구세포와 3T3-L1 cell의 증식과 분화에 미치는 영향)

  • Song, Mi-Yeon;Lee, Jae-Joon;Cha, Seon-Sook;Chung, Chung-Soo
    • Journal of Animal Science and Technology
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    • v.55 no.1
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    • pp.19-23
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    • 2013
  • The current study was carried out to determine the effects of Kohlrabi (Brassica oleracea var. gongylodes) on proliferation and differentiation of pig preadipocytes and $_3T_3-L_1$ cells. Pig preadipocytes were isolated from the backfat of the new-born pigs. Twenty-four hours after seeding, the cells were washed with DMEM/F-12 (designated day 0). To measure the cell proliferation, the cells were treated with 25 ng/ml and 100 ng/ml ethanol extracts of Kohlrabi (peel and flesh) for two days (day 0 ~ 2). To measure differentiation, the cells were treated with Kohlrabi for two days (day 0 ~ 2) and cell differentiation was measured on day 6. Twenty-five ng/ml and 100 ng/ml of Kohlrabi peel decreased proliferation of pig preadipocytes by 4.59% and 17.7%, respectively, compared with the control and Kohlrabi flesh by 11.4% and 19.2%, respectively. However, Kohlrabi did not inhibit cell differentiation. To measure the effects of Kohlrabi on proliferation and differentiation of $_3T_3-L_1$ cells, the cells were treated with Kohlrabi for two days in culture, like pig preadipocytes. Kohlrabi (both peel and flesh) did not show any effects on cell proliferation and differentiation. In summary, the results of the current study showed that Kohlrabi decreased proliferation of pig preadipocytes, but no inhibitory effects on differentiation of the cells. Kohlrabi had no effects on proliferation and differentiation of $_3T_3-L_1$ cells.

Effect of Fe3$^+$ on Differentiation of Chick Embryonic Myoblasts Cultured in nitro (배양계배 근원세포의 분화에 미치는 Fe3$^+$의 영향)

  • 유병제;지승완
    • The Korean Journal of Zoology
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    • v.34 no.4
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    • pp.610-617
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    • 1991
  • 계배 근원세포의 분화 및 증식에 미치는 F3+의 영향을 조사하였다. 철이 없는 배양액은 근원 세포의 분화와 증식을 억제하는 것으로 나타났으며 , 따라서 근원세포의 분화에 철과 transferrin (Tf)은 필수적이다. 또한, 철 대신에 Co2+가 부착된 Tf가 첨가된 배양액에서도 근세포의 분화가 정상적으로 일어나는 것으로 나타났다. Lysosomotrophic amine(chloroquine, $\mu$M 수준;ammonium chloride, mM 수준)은 근세포의 분화와 증식을 억제시켰으며 , 근세포 분화의 철에 대한 의존성은 분화됨에 따라 둔화되었다. 세포내로의 T니 수송량은 MEM과 8102배양액에서 비슷하였고, 근세포가 분화됨에 따라 감소하였다. Lysosomotrophic azine은 최소한 3시간이 내에서는 세포내로 수송되는 Tf의 양에 영향을 미치지 못하였다.

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Establishment of proliferation and regeneration system of PLBs in Phalaenopsis by treatments of a variety of types of medium, sucrose concentrations and anti-browning agents (다양한 배지종류, sucrose 농도 및 갈변억제물질 처리에 의한 팔레놉시스 PLB 증식 및 재분화 체계확립)

  • Roh, Hee Sun;Kim, Jong Bo
    • Journal of Plant Biotechnology
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    • v.41 no.4
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    • pp.223-228
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    • 2014
  • To establish an efficient proliferation and regeneration of PLBs (protocorm-like bodies) of Phalaenopsis plants, a variety of propagation medium types, various concentraions of sucrose as well as liquid and solid type were tested in this study. Further, activated charcoal, citric acid and ascorbic acid were compared whether these agents are suppose to reduce the browning in culture process using PLBs of Phalanopsis plants. With regard to the proper propagation medium, VW medium showed 1.3 ~ 2 times highr than those of other medium in an index of increasing for fresh weight and 50% higher than those of other medium in the frequency of shoot regeneration. However, regarding liquid and solid types of culture, there were no significant differences in the proliferation of PLBs and regeneration of shoots from PLBs. In the experiment for a variety of sucrose concentrations (0 ~ 50 g/l), 10 g of sucrose showed 30 ~ 50% higher than other concentrations in increasing index and 10 ~ 50% higher in the regeneration of shoots from PLBs. Regarding the reduction of browning in tissue culture via PLBs of Phalaenopsis plants, 1 g of activated charcoal showed only 1.5% browning of PLBs cultured. Whereas, other treatments including citric acid and ascorbic acid showed 6 ~ 16% of browning of PLBs. Therefore, activated charcoal was selected as an efficient anti-browning agents for the culture of PLBs in Phalaenopsis plants. Using above-described results can be contibuted to the establishment of mass propagation system using PLBs of Phalaenopsis plants in the future.

Micropropagation by Leaf and Meristem Cultures of Pelargonium citrosa Van leenen (구문초 (Pelargonium citrosa Van leenen)의 잎과 정분열조직배양에 의한 미세증식)

  • 은종선;고정애;김영선;김명준
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.4
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    • pp.247-252
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    • 1994
  • The effects of explant sources, plant growth regulators on callus induction and plantlet differentiation from leaf blade, petiole, and meristem tissue of Pelalgonium citrosa were investigated under illumination or in dark condition Leaf blade explants cultured on Murashige and Skoog's medium containing 2,4-D and kinetin did not form callus or organ. But those cultured on medium with NAA and BA produced callcus and shoots. Dark condition was more effective than light condition to callus induction and showed that some of shoot were differentiated directly from leaf blade explane. Callus proliferated vigorously on meristem tissue after 7 days of culture, and multiple shoots were obtained Sum callus on medium with 0.5 mg/L NAA and BA. Roots formed readily from about 80% of the shoots cultured on medium with 1.0 mg/L NAA. Regenerated plantlets regenerated had phenotypically normal leaves and roots.

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B lymphocyte에 관한 연구동향

  • 이헌구
    • The Microorganisms and Industry
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    • v.17 no.1
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    • pp.29-31
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    • 1991
  • B 세포이 가장 중요한 기능은 항체를 생산하여 체액성 면역반응을 조절하는 일이다. B 세포가 항체를 생산해 내는 형질세포(plasma cell)로 분화하기 위해서는 다음의 몇 가지 단계를 거쳐야 한다. 즉 resting 상태에서 mitogen이나 항원의 자극에 의한 활성화(activation)가 첫번째 단계이고 두번째로는 증식(proliferation)이며 마지막 단계가 분화(differentiation)이다. B 세포가 일단 mitogen이나 항원에 의해 활성화가 되면 활성화 된 clone의 증식과 분화는 T 세포에서 생산되는 여러 종류의 림포카인(lymphokine)의 영향하에서 이루어진다. 따라서 B 세포 기능에 영향을 미치는 림포카인의 종류를 알아내고 최근 새롭게 발견되는 림포카인의 B 세포에 대한 영향을 연구함이 중요하리라 생각된다. 여기에서는 B 세포 기능에 영향을 미치는 여러 림포카인의 종류의 그 기능에 관해서 간략하게 기술하고자 한다.

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Effects of Sex Steroid Hormones on Proliferation and Differentiation of Preadipocytes from Female and Male Pigs (스테로이드 성호르몬이 암, 수 돼지 지방전구세포의 증식과 분화에 미치는 영향)

  • Kim, Won-Young;Chung, Chung-Soo
    • Journal of Animal Science and Technology
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    • v.52 no.1
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    • pp.17-22
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    • 2010
  • The current study was undertaken to determine the effects of sex steroid hormones (estrogen, testosterone and 19-nortestosterone) on proliferation and differentiation of preadipocytes of female and male pigs. The preadipocytes were isolated from the backfat of new-born female and male pigs by collagenase digestion and cultured in the $CO_2$ incubator. The concentration of $10^{-7}M$ and 10-6M sex steroid hormones were treated to the cultured preadipocytes. Regarding the effects on preadipocytes proliferation, high concentration ($10^{-6}M$) of all the three hormones increased proliferation of female preadipocytes,and only estrogen and testosterone increased proliferation of male preadipocytes. Regarding the effects on preadipocyte differentiation, all the three hormones increased differentiation of pig preadipocytes, regardless of hormone concentrations and sex of preadipocytes. The degree of stimulation of cell differentiation by sex steroid hormones was greater than that of cell proliferation.

Inhibition of Proliferation and Neurogenesis of Mouse Subventricular Zone Neural Stem Cells by a Mitochondrial Inhibitor Rotenone (미토콘드리아 억제제 rotenone에 의한 쥐의 뇌실 하 영역 신경 줄기 세포의 증식과 신경 세포로의 분화 억제)

  • Park, Ki-Youb;Kim, Man Su
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1397-1405
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    • 2018
  • Mitochondria have multiple functions in cells: providing chemical energy, storing cellular $Ca^{2+}$, generating reactive oxygen species, and regulating apoptosis. Through these functions, mitochondria are also involved in the maintenance, proliferation, and differentiation of stem/progenitor cells. In the brain, the subventricular zone (SVZ) is one of the neurogenic regions that contains neural stem cells (NSCs) throughout a lifetime. However, reports on the role of mitochondria in SVZ NSCs are scarce. Here, we show that rotenone, a complex I inhibitor of mitochondria, inhibits the proliferation and differentiation of SVZ NSCs in different ways. In proliferating NSCs, rotenone decreases mitosis as measured through phosphorylated histone H3 detection; moreover, apoptosis is not induced by rotenone at 50 nM. In differentiating NSCs, rotenone blocks neurogenesis and oligodendrogenesis while glial fibrillary acidic protein-positive astrocytes are not affected. Interestingly, in this study there were more cells in the differentiating NSCs treated with rotenone for 4-6 days than in the vehicle control group which was a different effect from the reduced number of cells in the proliferating NSCs. We examined both apoptosis and mitosis and found that rotenone decreased apoptosis as detected by staining cleaved caspase-3 but did not affect mitosis. Our results suggest that functional mitochondria are necessary in both the proliferation and differentiation of SVZ NSCs. Furthermore, mitochondria might be involved in the mitosis and apoptosis that occur during those processes.

In vitro Multiple-propagation of Wolly Grass (Imperata cylindrica 'Rubra') (홍띠(Imperata cylindrica 'Rubra') 기관분화에 의한 기내대량증식)

  • Kang, In-jin;Kantayos, Vipada;Choi, Jong Young;Lee, Ye-Jin;Bae, Chang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.79-79
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    • 2019
  • 홍띠(Imperata cylindrica 'Rubra') 식물자원의 기내대량증식과 재분화식물체의 순화체계를 구축하고자 기내 재분화에 적합한 식물재료부위, 생장조절물질을 조사하고, 재분화 유식물체로부터 적정 순화조건을 구명하였다. 기본배지로 MS (Murashige and Skoog, 1962) 배지를 사용하였고, 배양은 $26{\times}2^{\circ}C$, $25{\mu}mol/m^2/s$, 14h/10h (day/night) 광조건 하의 배양실에서 수행하였다. 캘러스 형성은 뿌리 끝, 줄기절편, 생장점 부위 중생장점 부위에서 가장 양호하였고, 이 생장점 조직에 0.1 mg/L의 2,4-D와 2 mg/L의 BA를 처리하였을 때 양호하였다. 캘러스 증식은 0.1 mg/L의 2,4-D와 0.05 mg/L의 BA 배지, 0.05 mg/L의 2,4-D와 0.5 mg/L의 BA를 첨가한 배지 중 0.1 mg/L의 2,4-D와 0.05 mg/L의 BA 배지에서 양호하였고, 이들 캘러스로부터 신초 재분화는 0.01 mg/L의 NAA와 2 mg/L의 BA 처리에서 양호하였다. 초기 치상으로부터 실제 경과시간은 캘러스 유도에 19주간(2018. 03. 18~07. 27), 캘러스 증식 9주간(2018. 07. 27~09. 28), 신초 유도 11주간(2018. 09.28~12. 14), 순화에 10주간(2018. 12. 14~2019. 02. 23)에 걸쳐 진행하였으나 확립된 배양계를 적용하면 캘러스 유도 4주, 캘러스 증식 3주, 신초유도 및 증식 4주, 순화 7주 정도가 소요될 것으로 계측되었다. 순화는 다경줄기 형성후 MS배지를 멸균한 상토(버미큘라이트) 또는 종이포트로 교체하여 재분화식물체를 배양병에서 7주간 배양하고, 7주후에 배양병 뚜껑을 1/10 정도 1차 개방하여 1주일 후 3/10 정도 개방하여 2주간 경과한 후 컵포트(직경 6 cm)에 이식하여 성공적으로 활착시켰다.

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Callus growth and plant regeneration from hybrid embryo of L. longiflorum X L. elegans (L. longiflorum X L. elegans의 잡종 배로부터 캘러스의 증식 및 식물체 재분화)

  • Yoon, Eui-Soo;Kwon, Hye-Kyoung;Cho, Yi-Yun
    • Journal of Plant Biotechnology
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    • v.33 no.2
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    • pp.99-104
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    • 2006
  • This study was performed to investigate regeneration of plants differentiated from hybrid embryos between L. longifilorum Georgia and L. elegans Kakutanohikari. In addition, proliferation of callus and process of differentiation were investigated by histological observation. The germination of hybrid embryos was observed in 86 individuals from 48 slice cultures. Plant regeneration was effective on a medium supplemented with 1 mg/L HPh, and only callus proliferation was the highest in combination of 0.1 mg/L HPh and 1 mg/L BA. Also, plant regeneration was the most effective on a medium supplemented with 50 mg/L pyridoxine. We concluded that somatic embryos were formed from procambium of callus and proliferation of embryonic or proembryonic cells were stimulated with NAA from procambial cells.