• Title/Summary/Keyword: 주중

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Combination of Colony Formation and Congo Red Reaction for Detecting Intra- and Extra-Cellular Cellulolytic Activities (세포내외 섬유소 분해능 검출을 위한 Colony 형성과 Congo Red반응의 병용)

  • Lee, D.S.;Kim, H.K.;Pack, M.Y.
    • Microbiology and Biotechnology Letters
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    • v.12 no.4
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    • pp.305-309
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    • 1984
  • A selective medium which allows growth of only cellulolytic bacteria was developed. The medium composed of 0.5% carboxymethylcellulose (CMC), 0.005% yeast extract, minerals and agar. Colony formation on this medium indicates overall activities of cellulose utilization. A subsequent test with Congo Red dye could distinguish extracellular cellulolysis from intracellular type.

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A Study of Managing System and Plans on the School Records Classification Scheme in Korea (국내 학교기록물 분류기준표의 운영 실태와 개선 방안)

  • Sun, Yeo-Wool;Kim, Po-Ok
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.20 no.1
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    • pp.73-85
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    • 2009
  • The school records are having lots of problems in the records management by the reason that general teachers who belongs to an elementary and a secondary school, were producing and managing all the records, they don't have any knowledge on the records management system. in this paper, tried to consider the plan of the school records classification scheme and record management system under the research on the actual condition of the management system and problem features. The first plan for managing is to complete the system of business classification scheme, the standardization of records management and develop manuals and guidelines, and the second is the range expansion of managing and reinforcing the appraisal procedures.

Microbiological Studies on Feed Supplements (사료첨가제(飼料添加劑)의 미생물오염(微生物汚染)에 관(關)하여)

  • Park, Su Kyung;Tak, Ryun Bin
    • Current Research on Agriculture and Life Sciences
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    • v.4
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    • pp.132-140
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    • 1986
  • Eighty one products from 36 kinds of vitamin and mineral feed supplement collected during August, 1984 to February, 1985 were examined for microbiological contamination. In addition, 83 strains of coliform isolated from the samples were tested for the resistance to 8 kinds of antimicrobial drugs and distribution of R plasmid. General bacteria were detected in all of samples tested. Bacterial population was varied from less than 10 per gram of the sample to 1,400,000 per gram and 34 (42%) of 81 samples were contaminated with 100 to 1,000 cells per gram. Coliform isolation, which was more frequent in samples with larger number of general bacteria, was possible in 14 (17.3%) out of 81 samples tested and 6 (33.3%) out of 18 companies were coliform positive in their products. Forty one (49.4%) out of 83 coliform isolates were fecal coliform. The frequency of resistant strains was the highest to sulfadimethoxine (Sa) with 92.8% and followed by streptomycin (Sm, 67.5%), tetracycline (Tc, 50.6%), kanamycin (Km, 26.5%), chloramphenicol (Cm, 18.1%) and ampicillin (Am, 15.7%). No strain was resistant to nalidixic acid (Na) and gentamicin (Gm). The resistance frequency of fecal coliform strains were higher compare to non-fecal coliform strains. There were minimum inhibitory concentration (MIC) of $3,200{\mu}g/m{\ell}$ or higher in 7 strains to Am, 3 to Sm and 3 to Km, and 70 strains had MIC of $1,600{\mu}g/m{\ell}$ of higher to Sa while Tc had MICs from $1.6{\mu}g/m{\ell}$ to $400{\mu}g/m{\ell}$. All strains had MICs of $6.3{\mu}g/m{\ell}$ of lower to Na and $3.1{\mu}g/m{\ell}$ of lower to Gm. Seventy nine (95.2%) of 83 strains were resistant to one or more drugs tested. The most frequent resistance patterns were SaSm (14.5%) and followed by SaSmTc(12%), SaSmTcKm(8.4%) SaTc (8.4%) and SaSmKm (7.2%) ; total 19 different patterns were noted. Thirty two (40.5%) of 79 resistant strains were transferred all of a part of their resistance to Escherichia coli ML 1410. The frequency of transferable resistance was high in Am (100%) and Cm (80%) while low in Tc (38.1%), Sa (18.2%), Sm (17.9%) and Km (4.5%).

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Travel Pattern Analysis Using TCS Data and GIS in Korea (TCS 자료 및 GIS를 이용한 한국의 통행패턴 분석)

  • Kim, Jae-Hun;Chung, Jin-Hyuk;Choi, Min-Hwan;Chang, Hoon
    • Journal of Korean Society of Transportation
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    • v.26 no.3
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    • pp.75-84
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    • 2008
  • In 2002, the 5-day workweek policy was effective in Korea. As we have expected, the 5-day workweek policy has changed people's travel behavior during weekdays and weekends. Several studies have been done to understand these changes and impacts on transportation systems. However, these studies have only focused on travel pattern changes without considering spatial factors. Said in another way, although individual travel pattern changes are usually investigated, indices adopted cannot describe travel pattern changes in a proper way due to lack of the spatial distribution measure. This study aims to analyze travel change since the 5-day work week policy in effect using a new index (i.e. Travel Vector Index) developed in this study, which can explain travel pattern changes in terms of magnitude and spatial point of views. The new index uses a GIS technology and TCS (Toll Collection System) databases in Korea. The results in this study show that the index is very useful and reliable to measure the travel patterns changes. They are applied to TCS data set and the results show that the 5-day workweek policy significantly affects on travel behaviors.

Assessment of Pedometer Counts, Physical Activity Level, Energy Expenditure, and Energy Balance of Weekdays and Weekend in Male High School Students (남자 고등학생의 주중과 주말의 보행수, 신체활동수준, 에너지 소비량 및 에너지 평형 평가)

  • Shin, Hyun-Mi;Jeon, Ji-Hye;Kim, Eun-Kyung
    • Journal of the Korean Dietetic Association
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    • v.22 no.2
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    • pp.131-142
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    • 2016
  • The purpose of this study was to assess the physical activity and energy balance of weekdays and weekend in male high school students. Fifty healthy male high school students participated in this study. Anthropometric data were collected. Physical activity level (PAL) and energy intake for weekdays and weekend were calculated from a physical activity diary and food diary using the 24-hour recall method and interview. The resting metabolic rate (RMR) and estimated energy requirement (EER) were calculated from the prediction equations suggested in 2015 KDRIs. Total energy expenditure (TEE) was calculated by multiplying RMR by PAL. Mean age of subjects was $15.9{\pm}0.33years$. The daily pedometer counts were significantly higher in the weekdays (12,837 steps) than in weekend (6,661 steps) (P<0.001). The PAL of the weekdays ($1.63{\pm}0.17$) was significantly higher than that ($1.37{\pm}0.26$) of the weekend (P<0.001). PAL was significantly correlated with pedometer counts on the weekdays (r=0.495) and weekend (r=0.686). The total energy intakes ($2,847.2{\pm}681.5kcal$) and TEE ($3,046.3{\pm}437.3kcal$) of weekdays were significantly higher than those of the weekend. The results of this study would be useful to develop nutrition and exercise programs for male high school students on weekdays and weekend, respectively.

노출평가를 위한 TLV 근거 - 2-BUTOXYETHANOL(8)

  • Kim, Chi-Nyeon
    • 월간산업보건
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    • s.379
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    • pp.9-16
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    • 2019
  • 2-부톡시에탄올(2-butoxyethanol)에 대한 생물학적 노출지수 설정에 필요한 자료는 상당수 존재한다. 다양한 실험실 및 직업적 현장연구는 2-butoxyethanol에 노출된 후 소변에서 butoxyacetic acid의 배설을 조사하여 생물학적 노출지수를 구축할 데이터베이스를 제공하였다. 실험실 연구는 작업시간 동안보다는 짧은 시간을 노출시킨다. 휴식 중에 실시한 실험 자료들을 20ppm에 8시간 노출로 외삽하면 작업시작 종료 시점의 소변 중 butoxyacetic acid의 농도가 각각 192, 272, 190 mg/g creatinine이었다. 작업부하 30 watts와 50 watts의 연구 결과를 외삽하면 각각 304 butoxyacetic acid/g creatinine과 313 butoxyacetic acid/g creatinine이었다. 작업현장 연구는 소변을 가수분해시킨 후 총 butoxyacetic acid의 검출 범위가 광범위할 것으로 예상된다. 이러한 외삽의 결과는 일반적으로 1ppm 미만의 노출에서 나온 것이라는 점에 유의해야 한다. 피부 흡수의 가능성과 2-butoxyethanol의 상대적으로 낮은 휘발성을 감안할 때 현장 연구에서는 피부 경로가 흡입 연구보다 더 중요할 수 있다. 따라서 현장 조사는 휴식을 취한 자원봉사자를 대상으로 실시한 흡입 연구와 비교하면 소변 중 butoxyacetic acid 농도를 과대평가하는 경향이 있다. 활용 가능한 시뮬레이션 연구에서 휴식 시 2-butoxyethanol의 TLV-TWA 20ppm에 8시간 전신(흡입과 피부) 노출되는 경우 가수분해된 총 butoxyacetic acid가 210 mg/g creatinine이 예상되고 작업부하 50 watts에서는 373 mg/g creatinine이 예측된다. 50 watts의 작업량으로 20ppm에서 8시간 노출되는 경우 생물학적 노출지수(BEI)는 약 400 mg/g creatinine일 것이다. 그러나 생물학적 노출지수는 외삽에 대한 불확실성, 심각한 피부 노출 및 작업장 노출평가 자료의 부족을 반영하여 200 mg butoxyacetic acid/g creatinine으로 설정하였다. 인용한 자료들의 한계에도 불구하고 자원 봉사자 연구와 시뮬레이션 연구의 자료들이 일치성이 있었다. 연구에 따르면 butoxyacetic acid는 측정 가능한 양으로 존재하며 작업 교대 중에 검출될 수 있는 충분한 시간의 반감기를 가지고 있다. Sakai 등의 연구 자료와 일부 모델링 자료에서 노출농도가 높은 경우 주중에 약간의 축적 가능성을 보고하였지만 작업장에서 일반적으로 노출되는 농도 범위에서는 누적이 예상되지 않으며 작업 종료 후에 소변시료를 채취하는 것이 바람직하다. 대다수의 연구에서 소변 중 butoxyacetic acid 농도 수준의 개인 간 다양성을 보고하였다. 또한 대부분의 작업현장 연구에서는 2-butoxyethanol의 주변 환경 농도수준과 소변 중 butoxyacetic acid 수준에 유의한 상관관계를 발견하지 못하였다. 상호 연관성이 결여된 원인은 대부분 피부 노출의 영향으로 추정하였다. 개인 보호 장갑을 착용한 근로자들에게서는 유의한 상관관계가 발견되었다.

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Glutathione Sulphydryl Contents and Antioxidant Activities of Lactobacillus spp. and Bacillus coagulans (Lactobacillus spp.와 Bacillus coagulans의 Glutathione Sulphydryl 함유율과 황산화 활성)

  • Byun, Jeong-Yeol;Yoon, Yeong-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.22 no.2
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    • pp.91-97
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    • 2004
  • The antioxidative ability on the basis of reduced glutathione sulphydryl(GSH) level, the inhibition activities of linoleic acid peroxidation of cell free extract of Lactobacillus spp. and Bacillus spp. have been determined; Lactobacillus casei CU4114 contained the highest level of GSH among the probiotic strains with 25.15 ${\mu}$mole/g. Significantly high level of GSH occured in the intracellular cell free extract of Lactobacillus rhamnosus CU4201, Lactobacillus plantarum CU4203. The antioxidant activity and inhibition of linoleic acid peroxidation of cell free extract of Lactobacillus spp. and Bacillus spp. by thiobarbituric acid(TBA) assay have been shown to be significantly differed depending on the strains(P>0.01). Intracellular cell free extracts of L. acidophilus CU4111, L. casei CU4114, and strains of Bacillus coacillus revealed a significantly intensive inhibitory activity in the linoleic acid peroxidation reactions. Spearmans' rank correlation between inhibitory activity on linoleic acid peroxidation and cellular GSH levels of Lactobacillus spp. was analysed and the correlation quotient was 0.65 which means a significant positive correlation.

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Production of Monoclonal Antibody to Newcastle Disease Virus and its Diagnostic Use (뉴켓슬병 바이러스에 대한 단크론성 항체생산 및 진단에 이용)

  • Chung Ok Choi;Chung Gil Lee;Sung Man Cho;Jin Soo Na;Soo Hwan An;Joon Hun Kwon
    • Korean Journal of Poultry Science
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    • v.15 no.3
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    • pp.207-210
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    • 1988
  • A total of 3 hybridoma clones producting menoclonal antibody (MCA) against Newcastle disease virus(NDV) was raised by cell fusion method. The MCAs did not cross react against other avian or mammalian viruses tested. However, these antibodies reacted with all strains of velogenic and lentogenic NDVs tested indicating that they are unable to discriminate the possible antigenic differences among NDVs. All. the MCAs were classified as IgG type and did not show neutralizing and hemagglutination inhibition (HAI) activity except one clone which has low HAI activity. One of these MCA raised in mouse ascites revealed the titer of $10^6$ by indirect immunofluorescent antibody (IFA) test Using the MCA, virulent NDV could easily be detected from tracheal and conjunctival smears made 2 to 3 days after experimental infection.

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An Investigation of Fluid Mixing with Direct Vessel Injection (직접용기주입에 따른 유체혼합에 관한 연구)

  • Cha, Jong-Hee;Jun, Hyung-Gil
    • Nuclear Engineering and Technology
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    • v.26 no.1
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    • pp.63-77
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    • 1994
  • The objective of this work is to investigate fluid mixing phenomena related to pressurized thermal shock(PTS) in a pressurized water reactor(PWR) vessel downcomer during transient cooldown with direct vessel injection(DVI) using test models. The test model designs were based on ABB Combustion Engineering(C-E) System 80+ reactor geometry. A cold leg small break loss-of-coolant accident(LOCA) md a main steam line teak were selected as the potential PTS events for the C-E System 80+. This work consist of two parts. The first part provides the visualization tests of the fluid mixing between DVI fluid and existing coolant in the downcomer region, and the second part is to compare the results of thermal mixing tests with DVI in the other test model. Row visualization tests with DVI have clarified the physical interaction between DVI fluid and primary coolant during transient cooldown. A significant temperature drop was observed in the downcomer during the tests of a small break LOCA Measured transient temperature profiles agree well with the predictions by the REMIX code for a small break LOCA and with the calculations by the COMMIX-1B code for a steam line break event.

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Implementing Academic Information Systems from a Mindfulness Perspective (마음챙김 관점에서 본 대학 정보화 프로젝트)

  • Oh, Sang-Jo;Kim, Yong-Young;Kim, Beom-Soo
    • The Journal of Society for e-Business Studies
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    • v.16 no.3
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    • pp.225-247
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    • 2011
  • Universities have been investing in their information systems to keep their management functions working efficiently and effectively. Thanks to these efforts, faculty members and other university employees can perform their tasks efficiently almost anywhere and anytime. Several universities embarked upon information systems projects to take advantage of emerging information technologies in their administration and management. Only a limited number of universities have achieved their objectives; some others have been even tinkering with the idea of abandoning their newly developed academic information systems. The development of academic information systems often start with a relatively high risk of system failure. Sometimes, these systems encounter unforeseen obstacles or fail in systems development. These universities with problems also can overcome these issues by collecting their wisdom. The mindfulness theory is suitable for dealing with implementing academic information systems. This research analyzes an academic information systems development case from a mindfulness perspective, and also addresses its research implications and theoretical contributions.