• Title/Summary/Keyword: 주사형전자현미경

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Morphology, Biology and Chemical Control of Tomato Russet mite, Aculops lycopersici Massee (Acari: Eriophyidae) in Korea (토마토녹응애의 형태, 생태 및 방제)

  • 김동근;박덕기;김승한;박인숙;최성국
    • Korean journal of applied entomology
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    • v.41 no.4
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    • pp.255-261
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    • 2002
  • Tomato russet mite, Aculops lycopersici Massee (Acari. Eriophyidae) was recorded in Korea and their SEM observations, distribution, host range, development on different temperature, and chemical control method were studied. Tomato russet mite is a microscopic animal (134.9 $\mu\textrm{m}$) with 2 pairs of legs which had 4 rays of feathered craw. Infected leaf turn reddish brown, stem loss hair, become shiny or surface cross-cracked, and shoot wilted or shrunk. Surface of fruit around fruit stalk appeared rusted or cross-cracked. It was found in glass greenhouse at Buyeo and Yuseong in Chungnam Province, Pyeongchang in Gangwon Province, and Chilgok and Guryongpo in Gyeongbuk Province. Convolvulus, black nightshade, chinese lanternplant, eggp1ant, morning glory, petunia, potato, pepper, tobacco and tomato were confirmed as a host plant in inoculation test. It could develop at temperature around $15-30^{\circ}C$, it took 6-11 days from eggs to adult, higher temperature shorten its life cycle. Female lived longer than male and it lived as long as 12 days at 25-28"C. Among acaricides tested, fenazaquin and cypermethrin were most effective.tive.

Antimicrobial Characteristics of Scutellariae Radix Extract (황금추출물의 항균특성)

  • 조성환;김영록
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.964-968
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    • 2001
  • It was carried out for research and development of natural antimicrobial on Scutellariae Radix extract against food-borne infection bacteria .Scutellariae Radix extract showed remarkable antimicrobial activites against Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Vivro parahaemolyticus when examined by disk method, it was very stable on the wide rane of temperature and pH,.The growth rates of Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Vivro parahaemolyticus were decreased at the concentration of more than 500 ppm Scutellariae Radix extract, Indicating that the minimum inhibitory concentration (MIC)of the Bacillus cereus, Listeria monocytogenes, Escherichia coli, and Vivro parahaemolyticus to Scutellariae Radix extract were around 500 ppm . The morphological changes were observed by transmission electron microscope and scanning electron microscope and the microbial cells membran was destroyed by Scutellariae Radix extract. It was identified that the membrane integrity of the sensitive cells was disrupted by exposure to Scutellariae Radix extract as the $\beta$-galactosidase test on experimental substrate ONPG(o-nitrophenyl-$\beta$-D-galacto-pyranoside)

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Micromorphological Features of Oak Wood Cultivated With Shiitake Mushroom, Lentinus edodes(Berk) Sing (표고 재배 졸참나무 골목의 미관형태적(微觀形態的) 변화(變化) 양상(樣相))

  • Kim, Yoon-Soo;Park, Byung-Dae;Lee, Jae-Ki
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.3
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    • pp.38-47
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    • 1988
  • 표고버섯(Lentinus edodes) 균에 의한 졸참나무(Quercus serrata)골목의 미시형태적 변화를 주사형전자현미경 (SEM)을 통해 관찰하였다. 표고 재배 5년후 골목의 중량 감소율은 50%에 달했다. 구성세포중 목섬유는 표고균사에 의해 쉽게 분해되었으나 도관과 유세포는 저항성을 나타냈다. SEM 관찰 결과 표고균사는 세포내강(內腔)에서부터 세포벽을 서서히 분해 박벽화(薄壁化)시켰으며, 또한 Cell corner가 중간층보다 먼저 공격을 받아 분해 되었으나 중간층은 쉽게 분해되지 않았다. 흥미롭게도 연부후(軟腐朽)의 전형적 형태인 2차막에서의 공동(空洞)역시 백색부후균인 표고균사에 의해 형성 되었다. 이상의 관찰결과 표고균은 백색부후와 연부후균이 갖는 미시형태적 특징을 동시에 보여 주었다. 아울러 중간층 보다 Cell corner가 먼저 선택적으로 분해되며, 도관세포벽의 분해저항성을 세포벽 구성 리그닌의 화학적 특성과 연관시켜 논의했다.

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A Contribution to the Pollen Morphology of Salix viminalis L. (Salix viminalis의 화분형태(花粉形態)에 관(關)한 연구(硏究))

  • Kim, Kae Hwan
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.64-67
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    • 1984
  • The genus Salix is nowadays becoming increasingly important for both biomass energy production and the expanding paper industry. The enlarging plantations will however create some potential as well as problem. The great potential of genetical improvement as well as the prevention of pollen allergies requires knowledge about e.g, the pollen morphology of the most used Salix species. This study investigated the pollen morphology of Salix viminalis L. both on light microscope (LM) and scanning electron microscope (SEM).

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Fabrication of the photon scanning tunneling microscope with constant intensity mode (일정광량 방식의 광자주사현미경 제작)

  • Kim, Ji-Taek;Choi, Wan-Hae;Jo, Jae-Heung;Chang, Soo;Kim, Dal-Hyun;Koo, Ja-Yong;Chung, Seung-Tae
    • Korean Journal of Optics and Photonics
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    • v.10 no.3
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    • pp.195-200
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    • 1999
  • We made sharp optical fiber tips with less than 100 nm diameter by using the heating and pulling method with a good repetition and fabricated the photon scanning tunneling microscope (PSTM) using constant intensity mode. The 3-dimensional PZT (Piezoelctric transducer) scanner made of a long PZT tube is consisted of three divided parts, that is, a pair of $\pm$ x and a pair of $\pm$y scanning parts and a z scanning part for the fine approach and scanning. The scanning dimension is 1.43 $\mu\textrm{m}$$\times$1.76 $\mu\textrm{m}$. The height of a optical tip to maintain a constant height within $1/{\lambda}_0$ (${\lambda}_0$ is the incident wavelength) from surface of a specimen to a optical tip is controlled automatically by using the electric feedback circuit. The 3-dimensional shape of standing evanescent waves generated on the surface of a dove prism was measured successfully by using the constant intensity mode PSTM.

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Surface ultrastnlcture of metacercaria and adult of Gymnophalloides seoi (Digenea: Gymnnophallidae) (참굴큰입흡충의 피낭유충 및 성충의 표피 미세구조)

  • 최민호;박원진
    • Parasites, Hosts and Diseases
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    • v.33 no.4
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    • pp.289-296
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    • 1995
  • The surface ultrastructure of metacercariae and adults of Gymnophallokles seoi, the only known gymnophallid infecting humans, was observed by scanning electron microscopy. Metacercariae were ovoid or pyriform in shape and slightly concave ventrally. The oral sucker had two sizes of type I papillae, small and large, encircling its lip. Type I papillae were arranged in a row on both side oi the body. The ventral pit had several type I papillae on its inner surface. The ventral sucker was covered with cobble-stone like cytoplasmic processes and had 6 type I papillae on its lip. The surface of the body was covered with the tegumental spines except for the ventromedian area between the two suckers. The spines at anterior body were digitated into 3-5 points, and their size decreased at posterior one third or the body. Adult worms were rhomboid or ellipsoid in shape and covered with tegumental spines except for the ventromedian area. The shape and distribution of the tegumental spines and sensory papillae were similar to those of metacercariae. However, sensory papillae arranged in a row on the ventral surface of metacercariae were not observed in adults. The ventral pit became larger and more prominent as the fluke grew. It is suggested that the ventral pit function as an additional adhesive organ to the host tissue.

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Comparative seed morphology of the tribe Alsineae (Caryophyllaceae) in Korea and its taxonomic implications (한국산 Alsineae (석죽과) 종자의 형태비교 및 분류학적 유용성)

  • Song, Jun-Ho;Kim, Ju-Mi;Ok, Min-Kyoung;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.369-379
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    • 2015
  • The seed morphology of 25 taxa (8 genera) of Alsineae (Alsinoideae, Caryophyllaceae) in Korea was examined using stereo (SM) and scanning electron microscopy (SEM) in order to evaluate their diagnostic values and taxonomic implications. Circular, comma-shaped, ellipsoid, ovate, pyriform, reniform, and sub-circular seed shapes were identified. The seeds of the examined taxa range from 0.28 to 4.10 mm in length and from 0.33 to 3.10 mm in width; the smallest one was found in Sagina japonica, while the largest was in Honkenya peploides var. major. A hilar appendage was found in Cerastium, Honkenya, Moehringia, and strophioles were only observed in the genus Moehringia. Based on the examination of epidermal cells of the seed surface, four micromorphological types can be distinguished, viz., the colliculate-papillate (Col-Pap), colliculate-columellate (Col-Cll), colliculate-simple (Col-Sim), and smooth (Sm) types. Descriptions of the testa shape, margin, and the degree of elevation of the anticlinal cell walls, and the outer periclinal cell walls are summarized for the tribe. Taxonomic implications of the seed micromorphological characters were also discussed, in comparison with an earlier taxonomic system.

The surface morphology control of the polymeric material using a linear ion source (선형 이온 소스 적용을 통한 폴리머 소재의 표면구조 제어)

  • Sin, Min-Ho;Kim, Byeong-Jun;Park, Yeong-Bae;Kim, Do-Geun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.141.2-141.2
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    • 2016
  • 디스플레이, 센서 등 전자소자는 소형화 단계를 지나 인체 부착형 소자로의 발전을 요구하고 있다. 인체 부착을 위해서는 민감한 피부에 장시간 부착시 무해성과 탈부착의 자유로움이 요구되기에 기존의 화학물질을 활용한 접착 방식에서 개코도마뱀 또는 딱정벌레 발바닥에서 영감을 얻은 자연모사형 건식 접착 방식에 대한 연구가 활발하게 진행되고 있다. 폴리머를 이용하여 자연모사형 마이크로/나노구조 형성은 기계적으로 가공된 금형 몰딩과 후처리를 통한 매우 복잡한 공정이 요구된다. 본 연구에서는 이러한 복잡한 공정을 단순화하기 위해서 폴리머 소재에 플라즈마를 활용한 나노구조를 형성하는 방법을 소개하고자 하며, 건식 접착용 폴리머 소재(PMMA, PDMS)에 따른 표면구조 변화와 표면에너지 변화에 대한 연구를 수행하였다. 플라즈마 표면처리를 위해서 본 연구실에서 자체 개발한 선형이온소스를 활용하였으며 입사에너지, 노출시간, 사용가스에 따른 표면형상 변화를 주사전자현미경을 활용하여 관찰하였다. 또한 처리조건에 따른 표면에너지 변화를 확인하기 위해 물접촉각 변화를 측정하였다. PMMA는 입사에너지, 노출시간이 증가함에 따라 쉽게 나노기둥구조가 형성되었으나, 과도한 입사에너지 또는 노출시간에서는 표면구조가 에칭되면서 무너지는 것이 관찰되었다. 또한 PDMS는 동일한 조건에서 주름구조 형태를 보였으며 노출시간이 증가할수록 주름구조의 간격이 늘어남을 확인하였다. 본 연구 결과를 통해 나노 구조를 쉽게 제어할 수 있는 PMMA가 피부 부착형 접착 패치에 응용이 가능할 것으로 기대된다.

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Ultrastructure of the Fertilized Egg Envelope from Hyphessobrycon serpae, Characidae, Teleost (경골어류 카라신과 Hyphessobrycon serpae의 수정란 난막 미세구조)

  • Kim, Dong-Heui;Deung, Young-Kun;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.35 no.2
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    • pp.89-96
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    • 2005
  • The ultrastructures of the fertilized egg envelope from Hyphessobrycon serpae belonging to Characidae was studied using scanning and transmission electron microscopes to get systematic fundamental data for classification of species and to confirm whether micropyle is a common trait of Characidae or not. The fertilized egg was of colorless, transparent, spherical, adhesive and demersal type. There were not oil droplets in vitelline membrane and attached structures in the outside of fertilize egg envelope. The egg envelope had a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 13 to 15 protruded lines of the egg envelope in a radiated form. The outer surface of fertilized egg envelope was covered by reticular adhesive fibrous structures and irregularly arranged by pore canals. The fertilized egg envelope consisted of three distinct layers an outer adhesive fibrous layer with high electron density, a middle layer with pore canals, and an inner layer consisting of 6 to 7 lamellae alternating layers with interlamellae of lower electron density. These ultrastructural characters of fertilized egg envelope form Hyphessobrycon serpae can be utilized in taxonomy of teleost, and as fundamental data for study on early development of fertilized egg. It seems that the morphology of micropyle is a common trait of Characidae

THE EFFECT OF LASER IRRADIATION ON THE SURFACE CHARACTERISTICS OF TOOTH ENAMEL (레이저 처리가 치아 법랑질의 표면구조에 미치는 영향)

  • Lee, Ju-Hyun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.2
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    • pp.201-206
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    • 2006
  • A recent laboratory study has demonstrated improved caries lesion resistance with enamel that was exposed to $CO_2$ laser irradiation for very short time period. When topical fluoride treatment was performed before or after laser irradiation, reductions in dental caries. The purpose of this scanning electron microscopic(SEM) study and atomic force microscope study was to characterize surface alterations in tooth enamel after in vitro laser irradiation alone and combined topical fluoride treatment either before or after laser irradiation. The treatment effects of laser irradiation led to the formation of an irregular, mild porosities and fine fissures, also created granular materials. But when laser irradiation was followed by APF, the enamel surface had homogenous architecture. The result led to the caries resistance effects by these granular structures have been considered to represent redeposited mineral phases due to the mobilization of calcium, phosphate, and fluoride from lased enamel.

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