• Journal of The Korean Society of Grassland and Forage Science
• /
• v.30 no.3
• /
• pp.205-216
• /
• 2010

Few spring sowing have been conducted on winter cereal crops for whole-crop silage use. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were compared the spring sowing with the optimum season's sowing on growth, yield and feed quality in five winter cereal crops. The treatments consisted of 5 winter cereal crops, Youngyang (Barley, Spring habit I), Keumkang (Wheat, Spring habit II), Gogu(Rye, Spring habit estimated III), Shinyoung (Triticale, Spring habit estimated III), Samhan(Oat, Spring habit estimated II), and 3 planting dates, 18 October (optimum season's sowing), 23 February and 10 March in spring. Heading days as affected by spring sowing compared to optimum season sowing were delayed by 16~20 days in barley, wheat, rye and triticale, and 9 days in oat. The clipping dates at the optimal harvesting stage of each crop for round-baled silage in spring sowing was 8 June (yellow ripe stage) in barley, 25 May (10 days after heading) in rye, and 17 June in wheat (yellow ripe stage), triticale (milky stage) and oat (milky stage). The accumulative temperature from emergence to heading was significantly decreased as affected by spring sowing compared to optimum season's sowing, but that of sowing to emergence and that of heading to maturing was similar. The rate of spikes per tillering surveyed at each clipping date was 62.0-73.1 percent in barley, wheat, triticale and oat, and 56.0 percent in rye compared to that of optimum season sowing. The dry matter yield in spring sowing compared to 18 October was obtained about 71.7 percent in barley, 60.6 percent in wheat, 46.2 percent in rye, 70.2 percent in triticale and 110.9 percent in oat. It were increased in acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude protein content, but decreased in digestible dry matter content(DDM) and relative feed value (RFV). The yield of DDM by spring sowing was decreased in barley, wheat, rye and triticale, but increased in oat. The yield of dry matter and DDM were higher in oat and triticale than that of barley, wheat and oat. So, regardless to clipping dates and cropping system, the appropriated crop for spring sowing was oat, and subsequently triticale and barley. It was not adopted for spring sowing in rye because of low rate of no. of spikes per tillers and yield. It was necessary eliminated winter growing nature by earlier sowing at the late of February after overwinter.

• Research in Plant Disease
• /
• v.14 no.3
• /
• pp.143-152
• /
• 2008

Mixed-planting of two rice cultivars, HP ('Hopyeongbyeo') and NP ('Nampyeongbyeo'), having a dissimilar susceptibility to rice blast was practiced for chemical-free control of rice blast in the field. The HP/NP combination was selected for applying under mechanized agricultural conditions. Because they have similar genetic characteristics such as seed germination and heading time, culm length, rice quality and size of rice grains except susceptibility to blast. Incidence of panicle blast was reduced 50.4 % compare with supposed blast incidence by HP/NP mixed-planting when the seeds of two cultivars were combined 1 to 1 as weight. Supposed blast incidence was estimated from reduction of rice blast caused by addition of a resistant cultivar NP. Race diversity of Magnaporthe oryzae was examined for correlation with control effect of HP/NP mixed-planting on rice blast. The population of dominant race KJ-101 was diminished and replaced with various co-existing races and eleven new races were appeared in mixed-planting plot. Total number of race isolated from mixed-planting plot was not largely different from mono-culture. However, detection frequency of the new race was increased and variation of the population size of each race was decreased in mixed-planting plots. It was shown that a biased community with a dominant race (KJ-101 or KI-181) was altered to a balanced one of coexisting races. From these results, it was supposed that the balanced diversity among co-existing races within a community might be correlated to control effect by HP/NP mixed-planting on rice blast. Further more, it should be studied that genetic characteristics of the individual race including a virulence on cv. HP and NP was examined for verifying a correlation of mixed-planting effect and race diversity.

• Research in Plant Disease
• /
• v.14 no.3
• /
• pp.193-200
• /
• 2008

Blending of eggshell powder into soil as ratio of 1:5, 1:10, 1:15, 1:20, and 1:25 did not affect seed germination rates of several crops including Chinese cabbage. The blending increased pH of distilled water and decreased the viability of resting spores of Plasmodiophora hrassicae. The ratio of non-viable resting spores in eggshell-blending water was over five times higher than in distilled water of the same pH. Chinese cabbage (cv. 'Norangbom') grew more in eggshell-blended soil than in non-treated soil, but other crops grew less. Leaf numbers and above ground growth of Norangbom increased to around 150% and 470%, respectively, in soil blended with $1:20{\sim}1:15$ of eggshell powder. Even though the optimum sizes of eggshell powder were $0.8{\sim}2.0mm$ for growth and smaller than 0.4 mm for inhibition of clubroot disease of Chinese cabbage, there was no statistical difference among the sizes. Soil pH was above 8.0 in all eggshell treatments without any statistical difference among them. Eggshell powder blending to 1:20 showed lower control efficacy, 58.5%, than registered fungicide 'Hokanna (flusulfamide)', 78.5%. However, Chinese cabbage of that blending ratio recorded the highest growth among the treatments. Therefore, blending of eggshell powder into clubroot-contaminated soil may make culture of Chinese cabbage possible by growth-increasing, even though eggshell powder could not inhibit clubroot disease entirely.

• Korean Journal of Weed Science
• /
• v.9 no.2
• /
• pp.97-102
• /
• 1989

1. IAA showed no significant growth of root primordia and then lateral root emerged at 2 days after IAA treatment. BA treatment, however, strongly inhibited the formation of root primordia and a few lateral roots, if any, emerged about 5 days after treatment. 2. Treatment of BA and Indol-B on the water retaining ability sampled 1, 3, 5 days after chemical treatment was apparent on the soybean sprouts sampled 5 days after treatment while the difference among the treatments was negligible when sampled 1 and 3 days after treatment. 3. BA stimulated ABA content in the hypocotyl while inhibited ABA content in the root of soybean sprouts. ABA may relate with water retaining ability. 4. Soaking the soybean seeds to several seed disinfectant chemical solution had no effect on the growth and elongation of soybean sprouts. 5. It can be recommended that container of soybean sprouts should be shut tight during growing period except irrigation because the ethylene accumulation in the container may stimulate hypocotyl swelling and inhibit length of soybean sprouts.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.33 no.2
• /
• pp.112-121
• /
• 1988

Soybean seed coat consisted of three layers, and the aleurone layer was attached to the innermost part of seed coat. It showed the different morphological characteristics with single cell layer compared with many cell layers in barley aleurone layer. The structural difference in aleurone cell among varieties was not detected in this experiment. The hole of middle part of hilum consisted of net formed material in order to pass water and gas. In the experiment, it was not studied whether the varieties with hard seed consist of the same structure or not, but the detailed study on this would be necessary. The activity of acid phosphatase showed a tendency to increase in process of imbibition in distilled water. There was no significant difference in the enzyme activity among the varieties tested, but the enzyme activity of Danyoupkong was slightly higher than that of Hwanggeumkong. In germinability, Danyoupkong is higher than Hwangkeumkong, so it might be attributed to the higher activity. There was no difference in acid phosphatase activity between released from the aleurone cell and accumulated in the aleurone cell from 6 to 12 hours incubation of the medium in the absence of GA$_3$, but the difference was detected after 12 hours incubation. And enzyme activity was the highest in the 18 hours incubation. The increase in the release of acid phosphatase from the aleurone cell would be considered as passive diffusive effect due to the increase in turgo pressure of aleurone cell. The acid phosphatase released from aleurone layer increased greatly after 24 hours incubation of the medium in the presence of GA$_3$ and the accumulation within the aleurone cell decreased linearly after 18 hours incubation. The result indicates that GA$_3$ enhance the rate of enzyme release from aleurone layer, suggests that the aleurone cell wall be digested by the introduction of GA and the digested wall act as the channels for enzyme release.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.55 no.3
• /
• pp.187-194
• /
• 2010

The soybean stay green mutant genotype (SSG) derived from the nuclear gene, d1d2, and cytoplasmic gene, cytG, inhibit the breakdown of chloroplast in the leaves, pod walls, seed coats, and embryos during maturity. Soybean seed with black seed coat and green cotyledon (SBG) are preferred than black seed coat with yellow cotyledon (SBY) especially for cooking with rice and as source of traditional food in Korea. The researchers evaluated the seed's chlorophyll content of SSG and introduced SSG to the SBG variety breeding program. The seed chlorophyll content of SSG with d1d2 was $39.93{\sim}60.80\;{\mu}g/g$ and SSG with cytG $38.08{\sim}39.89\;{\mu}g/g$. The Korean SBG variety which was derived from SSG with cytG, contains $16.35{\sim}37.73\;{\mu}g/g$. The composition of seed chlorophyll differs according to the genetic background of SSG genotype. Inheritance study showed that cotyledon color was segregated 15:1 (yellow:green) at $F_2$ seed indicating two recessive genes control green cotyledon as revealed by previous study. Only less than 3% soybean lines showed black seed coat with green cotyledon among crosses SBY and SSG (d1d2). Results showed that SSG with d1d2 can be used as a good source for SBG with high chlorophyll content in the seed cotyledon, but due to the complex genetic behavior, breeding resource of SBG with d1d2 should be prepared to improve the breeding efficiency for development SBG variety.

• Applied Microscopy
• /
• v.36 no.3
• /
• pp.173-182
• /
• 2006

Recently, the pomegranate seed oil (PSO) has been reported to have various efforts including anti-cancer effect. In this study, we examined the liver-protecting effect of the PSO on the hepatotoxicity induced by $CCl_4$ using the BALB/c mice. The PSO was made from dried seeds of black pomegranate (Punica grantum) by heating and squeezing. The expreimental animals were divided into 3 groups; control group treated with olive oil only, experimental group 1 treated with $CCl_4$ only, and experimental group 2 treated with PSO and $CCl_4$. 24 hours after injection of $CCl_4$ into the peritoneal cavity, we collected the blood samples to measure the level of serological factors; aspartate aminotransferase(AST), alanine aminotransferase (ALT), total protein, albumin, total bilirubin, direct bilirubin and alkaline phosphatase. Simultaneously we observed the histological change of liver under the light and electron microscope. As the result, AST and ALT showed $88.7{\pm}14.9IU/L\;and\;22.0{\pm}3.12IU/L$ in the control group, $1963.7{\pm}1212.9IU/L\;and\;4495.4{\pm}2803.6IU/L$ in the experimental group 1, and $432.2{\pm}260.1IU/L\;and\;692.3{\pm}433.1IU/L$ in the experimental group 2. The experimental group 2 showed significant difference as compared with experimental group 1 (P<0.005). In histological study, the experimental group 2 was recovered than experimental group 1 which had abnormal mitochondria, increase of lysosomes, and severe necrosis at the central vein zones. These results indicated that the PSO had the liver protecting effect. However, The further study on the relationship between ingredients of pomegranate seed and liver protecting effect is in need.

• Horticultural Science & Technology
• /
• v.34 no.6
• /
• pp.959-965
• /
• 2016

In 1994, a new cultivar 'Joyskin' was created from a cross between the cultivars 'Whangkeumbae' and 'Waseaka' at the Pear Research Institute of the National Institute of Horticultural and Herbal Science, Rural Development Administration. In 2006, the 'Joyskin' was selected from among the 317 seedlings resulting from the cross for its skin and taste qualities. Regional adaptation tests were conducted in nine regions and in ten experimental plots from 2006 to 2011. The cultivar was named in 2011. 'Joyskin' showed a vigorous growth habit and semi-spread characteristics similar to 'Whangkeumbae'. The average full bloom date for 'Joyskin' was April 21st, which was also similar to 'Whangkeumbae'. The optimum fruit ripening time was September 6-8th, which was six or eight days earlier than 'Whangkeumbae'. The fruit was round in shape and the skin was a golden yellow color at maturity. The average fruit weight was 320 g and the flesh firmness was $2.5kg/8mm{\varphi}$. The firmness of the fruit skin determined by a blade-type plunger of texture analyzer was 22.9 N, which was significantly different from that of 'Whangkeumbae' 29.9N. Stone cell analysis of 'Joyskin' by phloroglucinol-HCl, showed that 'Joyskin' stone cells were small in size and few in numbers cpmpared to those of cultivars of was 'Manpungbae', 'Niitaka', and 'Whangkeumbae'. The patent application for 'Joyskin' was submitted in April, 2012 (Grant No. 2012-337). In 2016, 'Joyskin' (Grant No. 5895) was registered as a separate record, with uniformity and stability per Korean Seed Industry Law.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.24 no.4
• /
• pp.341-346
• /
• 2004

To determine lethal temperature of birdsfoot trefoil(BFT) and italian ryegrass(IRG) at heat-stressed conditions, seedlings grown in a small pots fur 4 weeks were subjected to different temperature regimes of heat treatment. No apparent damage was observed BFT and IRG were treated at 45, 50 or $60^{\circ}C$ for 1 h. And also heat treatments at 60, 65 and $70^{\circ}C$ for 1 h, both of them were withered and showed damage symptom on their leaves but it was not lethal conditions for the whole plants. By contrast, most of plants were prominently withered within one day after heat treatment at $80^{\circ}C/60min$. When BFT was exposed to $80^{\circ}C/60$ min, they were died within 6 days but there was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 55 min. IRC was also died within 2 days that exposed to $80^{\circ}C/20$ min but there was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 15 min. These results indicate that heat killing temperatures of BFT and IRG plants are $80^{\circ}C/60$ min an $80^{\circ}C/20$ min respectively. Simple viability assay system established in this study will be useful for selection and characterization of heat-tolerant transgenic BFT and IRG plants.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.22 no.2
• /
• pp.123-130
• /
• 2002

A field experiment was carried out to determine the effect of application level of animal manure on the nitrate nitrogen concentration, sugar content and animal intake of forage sorghum $\times$ sudangrass hybrid (Sorghum bicolor (L.) Moench, cv. Pionee. 988) in 1995. The application amount of animal manure were 50, 100 and 150MT in cattle manure, 20, 40 and 80MT in swine manure, and 10, 20 and 40MT/ha in poultry manure. Non-application plot(control) was involved. The nitrate nitrogen concentration was increased with increasing of application level of animal manure(P<0.05). Average nitrate nitrogen concentration was 397, 512, and 609mg/kg at low, medium and high application level of animal manure. The nitrate nitrogen concentration by plant height was 438mg/kg at 50~60m of plant height, 454mg at 100~120cm, and 418mg at 200~220cm. The nitrate nitrogen concentration of stems was 376mg, and significantly higher than that(135mg) of leaves(P<0.05) regardless of animal manure type, and lower parts of stems and leaves were significantly higher than those of upper parts of plants(P<0.05). Average nitrate nitrogen concentration of leaves was 151mg at lower, and 58mg at upper parts of plants, and the concentration of stems was 357mg at lower, 511mg at middle, and 610mg at upper parts of plants. The sugar contents of sorghum $\times$ sudangrass hybrid was decreased with increasing of application level of animal manure(P<0.05). Average sugar content was 4.9, 4.4, and 4.3。 at low, medium and high application level of animal manure. The sugar content by plant height was 3.9。 at 50~60 and 100~120cm of plant height, and 6.1。 at 200~220cm of plant height. Animal intake of sorghum $\times$ sudangrass hybrid was decreased greatly with increasing of application level of animal manure. Average intake was 73.9, 55.7, and 52.3% at low, medium and high application level of animal manure. The intake by animal manure type was 73.7% in cattle, 59.7% in swine and 62.5% in poultry manure.