• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Pediatric Infection and Vaccine
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• v.25 no.1
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• pp.26-34
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• 2018

Purpose: This study aimed to investigate the changes in the occurrence of rotavirus gastroenteritis (RGE) after the introduction of rotavirus vaccine and estimate rotavirus vaccine effectiveness in hospitalized children. Methods: We compared the retrospective data of 671 patients with acute gastroenteritis (AGE) admitted to the Department of Pediatrics, Hanyang University Seoul Hospital from January 1, 2014, to December 31, 2015, with retrospective data of 1,243 patients admitted to the same institution with AGE from January 1, 2004, to December 31, 2005. The vaccine effectiveness was estimated using a case-positive control test-negative study. Results: The proportion of RGE in AGE was significantly lower in 2014 to 2015 (9.0%, 48/531) than in 2004 to 2005 (22.7%, 282/1,243) (P<0.001). In particular, there was a significant decrease in the 6- to 11-, 12- to 23-, and 24- to 35-month-old groups (P<0.001), whose rotavirus vaccination rates were higher than the remaining age groups. The monthly distribution of patients with RGE in 2004 to 2005 was higher from November to May, peaked in January, followed by December and February. In 2014 to 2015, the monthly distribution of patients with RGE slightly peaked in January. In 2014 to 2015 study, the complete rotavirus vaccination rate was 66.0% (332/503) and incomplete vaccination rate was 6.2% (31/503). Presumed rotavirus vaccine effectiveness was 83.3% (95% confidence interval [CI], 60.5% to 92.9%) in the complete vaccination group and 27.4% (95% CI, -163.7% to 80.0%) in the incomplete group. Conclusions: The proportion of RGE in AGE has markedly decreased since the introduction of rotavirus vaccines, and improving vaccination coverage will further reduce the burden of RGE in Korea.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.95-102
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• 2013

Binary trans-activation (pOp/LhG4) system is one of the regulatory systems of transgene expression. The target gene expression is achieved by crossing the reporter plants with an activator in this system. In this study, we used the features of this system in Chinese cabbage as a way to protect genetic resources and new varieties. To establish pOp/LhG4 system in Chinese cabbage, we designed an activator (35SLhG41300), and reporter constructs (pOpGUSBart) and co-transformed using Agrobacterium. The transgenic plants were selected by antibiotics and the functional activity of pOp/LhG4 system was confirmed by GUS expression. To induce the tissue-specific function, we constructed pOp/LhG4 system (795LhGBart) using female tissue specific promoter (ProAt1g26795) of Arabidopsis. Co-transformed transgenic plants clearly showed tissue specific expression in Arabidopsis. The results suggest the possibility of the system's application of $F_1$ generation can be restricted by expressing the target gene to protect a new variety and genetic resource in Chinese cabbages.

• Journal of Animal Environmental Science
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• v.13 no.3
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• pp.161-170
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• 2007

Ozone from a pilot-scale ozone generator was treated on fermented pig liquid manure stored in a storage tank in order to reduce odor substances during the process of fermented liquid manure production. The group of ozone treatment showed one less than the organic matter compared that of the control. The preferable condition for characteristic changes was when the ratio of BOD to COD was less than 1.5. Ozone treatment showed better oxidizing power than control as it removed more suspended solids and had less methyl isobutyl ketone(P<0.05). Odor reduction measured by olfactory method was higher in ozone treatment than in control.

• Korean Journal of Food Science and Technology
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• v.7 no.3
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• pp.120-127
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• 1975

Despite the extensive study of protein effect to man and domestic animals, protein effect of sunflower seed oil cake to the growth and value of erythrocyte in rats is still remained to be investigated. This study is to investigate the effect of protein of sunflower seed oil cake to the rat growth and value of erythrocyte as well as the liver weight, heart, kidneys and spleen of rats. Sixty to seventy-days-old, male Sprague-Dawley rats weighing 50-60g, were used in this experiment and 36 rats allotted to 4 groups: standard, 30% cake, 20% cake and low protein. The standard group was fed for 7 weeks on the ration supplemented with 17% casein, 30% and 20% cake groups with sunflower seed oil cake by 30% and 20%, respectively, and low protein group without any supplementation. To observe value of erythrocyte, blood was taken from heart. Value of erythrocyte, hemoglobin and hematocrit value were determined by usual method and oxygen consumption by the method of Watts and Gourley. The result of this experiment suggests that it is recommendable to supplement deficient amino acids in animal.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.6 no.4
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• pp.296-301
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• 2005

This study was conducted to get the basic data on an effect of food waste compost for rock cut-slopese vegetation. Two foreign cool-season grasses and native plants were used for this experiment. Cool-season grasses were Festuca ruba and Lolium perene, Native plants were Amorpha fruticosa, and Indigofera pseudo-tinctoria. Amorpha fruticosa, Indigofera psendo-tinctoria, and Lolium perenne decreased in germination rate and plant height at NaCl concentrations of $0.4\%$ or over suddenly. Festuca rubra occurred to the sudden growth hindrance at NaCl concentrations of $0.2\%$ or over. Amorpha fruticosa and Indigofera pseudo-tinctoria appeared for germination of $45\%$ at all experiment plots. Lolium perenne increased in plant height as trial rate of food waste compost was abundant. Ground cover rate was the highest in Mixture III by $89.3\%$ and was fluctuated from $47.0\%$ after 30 days to $64.1\%$ after 45 days in Mixture 1. Descending order of ground cover rates in 4 treatments was Mixture III, Mixture IV, Mixture II, and Mixture I. As the growth

• Journal of Korean Society of Forest Science
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• v.78 no.2
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• pp.189-197
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• 1989

This study was carried out to produce the grafts of Juglans sinensis by juvenile grafting method which epicotyl of newly germinated seeds were used as stocks and juvenile fresh shoots were used as scion. The results obtained were as follows ; 1. When plastic tube installed covering up seed with soil up to 6cm height for diameter increment of epicotyl, the epicotyl can be grown up to thickness of 10mm. 2. When the soft fruit branches and the soft water sprout with the terminal bud 8cm to 12cm long were used as scions, the survival rates showed 90 Percent. 3. The optimum date for making juvenile grafts was around the 20th of May, and the survival rates of grafted seedlings showed 86 percent in average. 4. The grafted seedlings showed first sprouting the 15th of June, that is 25 days after making graft, and the sprouting rate was 72 percent. 5. The height-growth of grafted seedlings finished at the end of July, and diameter growth lasted into the end of October. 6. There was positive correlation between the height of grafted seedlings and the diameter at root collar. 7. In general, it takes two years to make plantable graft seedlings from hardwood scion and stock, but the juvenile graft seedlings can be easily obtained in a year and so it seems to be economic.

• Korean Journal of Breeding Science
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• v.43 no.2
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• pp.145-153
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• 2011

To develop new varieties of rapeseed (Brassica napus L.), the seeds of three varieties 'Naehan', 'Tammi', and 'Halla' were treated with proton ion beams and gamma rays (0~2,000 Gy), and then the characteristics of the mutants induced were examined up to $M_5$ generation to select the lines with fixed useful traits. In $M_5$ generation, we had selected several lines that were highly fixed for some useful traits such as plant height, maturity and flower size; one line with both earlier maturity and shorter stem than wild type, one line with only earlier maturity, two lines with shorter stem, one line with large flower, and one line with chlorophyll mutation. Among them, NP600-1-1-198-2 (induced from variety 'Naehan' was treated with proton ion beams 600 Gy) was superior for its distinction from the original variety, uniformity and stability. The unique characteristics of NP600-1-1-198-2 were dark green leaves, green stem, yellow flower, and black seed coat. Its flowering date was April 14, eight days earlier than its original variety, while seed maturity date was June 16 (five days earlier) and plant height 105 cm (shorter by 10 cm). NP600-1-1-198-2 has 52 silique per panicle, 6.2 cm silique length, 23 seeds per silique, 4.2 g per 1000 seeds and 45.9% oil content. The seed oil contained 67.8% of oleic acid, 16.7% of linoleic acid and 7.3% of linolenic acid but no erucic acid.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.303-307
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• 2008

We bred a new green-kerneled glutinous rice variety that can be cultivated in the whole area of Korea, because only one native green-kerneled glutinous rice cultivar, "Saengdongchalbyeo", has been cultivated in the southern coastal area due to its late heading. The seeds of "Saengdongchalbyeo" were irradiated with 200 Gy of gamma ray in 1995. A promising mutant variety, "Nogwonchalbyeo" ("Wonnong 17") was selected through line selection and regional yield trials. In particular, the new variety revealed at the earlier mid of August compared to that of "Saengdongchalbyeo", the early of September, and it was considerably tolerant to a field lodging due to its shortened culm length. Also, "Nogwonchalbyeo" had a higher ripened grain ratio and 1,000 grain weight compared to the original variety. The brown grain yield of the new variety was about 5.40 MT/ha, which was 11.3% higher than that of the original variety, in the regional yield trials at 3 different fields during 2000~2001. The brown and milled grains of the new rice variety contained 20 to 65% higher amount of total amino acids, respectively than that of the original and two checks. For chlorophyll -a, -b and total chlorophyll, the new variety showed nearly two-fold higher than the checks, and for the carotenoid, it had 5.3 - 7.6 times higher amount. These results showed that the new variety can be cultivated as a special green-kerneled glutinous rice with high functional compounds.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.328-331
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• 2009

The white flowered 'Eorayeon 2ho' is a cultivar of Lilium ${\times}$ formolonga developed in Department of Horticultural Sciences, Kangwon National University, Chuncheon, Korea. $F_1$ hybrid LA-77 was selected from the cross between a selected selting line($S_3$) of L. longiflorum 'Lorina' and a selfing line($S_3$) of L. ${\times}$ formolonga 'Augusta' in 2000. The $F_1$ hybrid LA-77 was backcrossed by a selected line of L. ${\times}$ far molonga 'Raizan No. 1' from 2001 to 2002. 'MF-No.2' was preliminarily selected for its upward flowering habit and high plant height. The evaluation of characteristics was investigated from 2003 to 2004 in Chuncheon and Yungweol, Kangwon province. 'Eorayeon 2ho' blooms in early July at outside, with high resistance to Botrytis and showing upward flowering habit. The plant height is 132.6 cm and the percentage of seed germination is 95.3%. Mean tepal length and width are 16.0 em and 4.3 cm, respectively. The length and width of leaf is 12.3 and 3.2 cm, respectively. This seed propagating cultivar shows high plant height suitable for a cut flower.