• The Korean Journal of Mycology
• /
• v.29 no.2
• /
• pp.110-115
• /
• 2001

Nine species of Bipolaris, two species of Drechslera and two species of Exserohilum were identified from 66 isolates collected from leaf spots and leaf blights on gramineous hosts of field crops, grass pasture plants, turfgrasses and wild weeds in Korea in 1998. They included B. bicolor, B. coicis, B. cynodontis, B. maydis, B. oryzae, B. panici-miliacei, B. setariae, B. sorghicola, B. sorokiniana, D. dictyoides, D. graminea, E. oryzicola and E. turcicum. Of these, B. bicolor, B. sorghicola, D. dictyoides, and E. oryzicola were recorded for the first time in Korea.

• The Korean Journal of Mycology
• /
• v.46 no.4
• /
• pp.405-414
• /
• 2018

Endophytic fungi were isolated from the leaves of diverse plants inhabiting Jeju Island, Korea. The fungal isolates were identified through phylogenetic analyses incorporating nucleotide sequences derived from the internal transcribed spacer region, large subunit region of ribosomal DNA, and beta-tubulin gene. Our results identified six endophytic fungi previously unknown in Korea namely, Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, and Zalerion arboricola were identified. Here, we present their cultural and morphological characteristics and phylogenetic relationship.

• The Korean Journal of Mycology
• /
• v.49 no.1
• /
• pp.81-86
• /
• 2021

We isolated endophytic fungi from Smilax china and Cryptomeria japonica. These fungal strains were identified based on their morphological characteristics and phylogenetic analyses of their internal transcribed spacer, large subunit rDNA, and translation elongation factor 1-α DNA. Among them, we discovered two fungi belonging to the genus Phyllosticta, which have not been previously recorded in Korea. We have described these two fungal strains, Phyllosticta ericarum 19E458 and Phyllosticta philoprina 19E012 in this study.

• Microbiology and Biotechnology Letters
• /
• v.38 no.3
• /
• pp.263-272
• /
• 2010

One hundred sixty three strains showing different colony morphological characteristics on different concentration of marine agar (MA) plates were isolated from ambient seawater near Dokdo island. Bacterial diversity and distributions were studied by phylogenetic analysis of the partial 16S rRNA gene sequences. One hundred sixty three strains were partially sequenced and analyzed phylogenetically. They were composed of 5 phyla, of which gamma-proteobacteria (58%), alpha-proteobacteria (20%), bacteriodetes (16%) were predominant. They were affiliated with 90 species. The 16S rRNA sequence similarity of the isolates was in 93.3 to 100 % range to reported sequence data. Thirty six isolates of among them were assumed to be novel species candidates based on similarity analysis of the 16S rRNA gene sequences. Overall, Proteobacteria and Bacteriodetes of the Dokdo coastal sea water showed a high diversity.

• Applied Biological Chemistry
• /
• v.32 no.2
• /
• pp.143-147
• /
• 1989

The volatile compounds of McIntosh apples were separated and identified at the stage of climacteric maximum. 21 compounds were identified from head space method and classes of that were 13 kinds of esters, 6 of alcohols, an aldehyde and a ketone. From simultaneous steam distillation-extraction method, 37 compounds were identified and classes of that were 20 kinds of esters, 14 of alcohols, 2 of aldehydes and a ketone. Esters were the most abundant flavor component in the both methods and next was alcohols.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2017.10a
• /
• pp.68-70
• /
• 2017

본 논문에서는 결절종을 추출하는 과정에서 비선형 퍼지 스트레칭 기법과 FCM 기반 양자화 기법을 적용하여 결절종을 추출하는 방법을 제안한다. 제안된 결절종 추출 방법은 비선형 형태의 퍼지 스트레칭 기법을 적용하여 명암 대비를 강조한 ROI 영역에 Monotone Cubic Spline기법과 FCM 기반 양자화 기법을 적용하여 Monotone Cubic Spline기법이 적용된 상단 부분을 분리한다. 분리된 상단 영역들에서 결절종이 명암도가 낮고 타원 형태를 가진다는 형태학적 특징을 이용하기 위해서 침식 기법을 적용하여 결절종의 후보 영역을 추출하고 8 방향 윤곽선 추적 알고리즘을 적용하여 잡음 영역을 제거한다. 잡음이 제거된 결절종 후보 영역에서 최종 결절종 영역을 추출하기 위해 라벨링 기법을 적용한다. 제안된 결절종 추출 방법의 성능을 분석하기 위해서 필립스 초음파 장비를 이용하여 20명 환자에서 획득한 20장의 영상을 대상으로 실험한 결과 기존의 방법보다 TPR(Ture Positive Rate)이 높게 나타나는 것을 확인하였다.

• 한국해양학회지
• /
• v.30 no.6
• /
• pp.529-536
• /
• 1995

A picophytoplankton has been isolated from the western channel of the Korea Strait. The cell was isolated by dilution method. It is about 2 ${\mu}m$ in diameter and has smooth surface. Organelles of nucleus, chloroplasts, mitochondrion, Golgi body, pyrenoids, vacuoles and lipid bodies are identified. Pigments are composed of chlorophyll a and chlorophyll b, ${\beta}$-carotene and other xanthophylls. Based on the ultrastructural features and pigment composition, it may belong to chlorococcalean picoplankton.

• Microbiology and Biotechnology Letters
• /
• v.47 no.4
• /
• pp.596-602
• /
• 2019

In September 2017, the rhizospheric soil of Tetragonia tetragonoides (Pall.) Kuntze was further sampled. One hundred and thirty eight species of microorganisms were isolated from the soil. Indole-3-acetic acid (IAA) production, siderophore production, and phosphate degradation were examined in order to confirm bacterial growth from isolated microorganisms. As a result, most strains were able to produce auxins or siderophores and to solubilize phosphate. In addition, 138 isolated strains were treated with tobacco extract and conferred pathogen resistance to host plants upon treatment. As a result, 35 strains that were able to reduce pathophysiology by more the 60% were selected. Among them, 6 strains with high induced systemic resistance (ISR) activity were found. All of these strains belong to the genus Bacillus according to the 16S rDNA sequence analysis. Bacillus aryabhattai KUDC6619 showed outstanding effects with reduced infection in tobacco and pepper plants. Probably, these Bacillus species play a beneficial role by association with T. tetragonoides for its survival in the harsh conditions found on the island of Dokdo.

• Journal of Life Science
• /
• v.21 no.11
• /
• pp.1619-1624
• /
• 2011

Endophytic fungal strains were isolated from the roots of six species plants in the Dokdo islands. Native plant samples, such as Artemisia japonica, Chenopodium album and Solanum nigrum were isolated from Dongdo, and those such as Cyrtomium falcatum, Dianthus longicalyx and Tetragonia tetragonoides were isolated from Seodo. In total, thirty two fungal strains were isolated from these native plants. To identify the fungal strains, polymerase chain reaction (PCR) amplification of internal transcribed spacer (ITS: containing ITS1, 5.8s and ITS2 region) regions was done with universal primers ITS1 and ITS4. Endophytic fungi of four species were isolated from A. japonica, eight species from C. album, three species from S. nigrum, three species from C. falcatum, three species from D. longicalyx and eleven species from T. tetragonoides. Culture filtrates (CF) of isolated endophytic fungi were used to treatwaito-c rice seedlings to test plant growth-promoting activity. As a result of bioassay, Ca-5-2-2 strain isolated from C. album expressed highest plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp., Fusarium sp. and Aspergillus sp. were the most abundantly distributed fungal strains in the six plants used in this study.

• Korean Journal of Food Science and Technology
• /
• v.28 no.2
• /
• pp.260-266
• /
• 1996

This study was carried out to investigate the bacterial microflora of commercial and traditional Kochujang. Computer aided idetification systems were used to identify the bacteria in Kochujang. Seven genera such as Bacillus, Corynebacterium, Enterococcus, pasteurella, Pseudomonas, Staphylococcus and Streptococcus in commercial process and 10 genera such as Bacillus, Corynebacterium, Enterococcus, Flavimonas, Flavobacterium, Gemella, Pasteurella, Pseudomonas, Staphylococcus and Streptococcus in traditional process were identified. Distribution of genus Bacillus was $56{\sim}70%$ during fermentation in the commercial process and $38{\sim}50%$ in the traditional process. B. lichenoformis strains identified in this study were classified into three types by their physiological characteristics.