• Ocean and Polar Research
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• v.36 no.1
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• pp.39-47
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• 2014

The black scraper Thamnaconus modestus was a commercially important fish species in the 1980s, but suddenly its commercial significance decreased in the 1990s mainly due to continuous overfishing. Recently, in order to reverse the depleted stocks of the black scraper and help the species recover, seed production technology has emerged. This has led to the farming of the black scraper in several parts of the southern coast of Korea. Since detailed research on its metabolism in relation to water temperature has been scanty, this was the investigative focus of the present study. The standard metabolism rates of the black scraper (9-10 months old, total length=$22.6{\pm}0.8cm$, wet weight=$140.3{\pm}13.9g$) were measured at seven different water temperature settings (12, 15, 17, 20, 23, 26, $28^{\circ}C$) to understand the relationship between metabolism and water temperature. Relationships between water temperature (WT) and oxygen consumption rate (OCR) were obtained as SOCR (weight-specific oxygen consumption rate) = 0.0117WT - 0.0135 ($r^2=0.9351$) and IOCR (oxygen consumption rate per individual) = 1.8160WT - 5.4007 ($r^2$ = 9428). The $Q_{10}$ (temperature sensitivity), an indicator of the sensitivity of biological function to temperature, was analyzed. In our experiment, when the water temperature increased, the $Q_{10}$ value decreased. The $Q_{10}$ value was 6.27 in waters where the temperature ranged from $12-15^{\circ}C$ and this was much higher than the values obtained in waters where temperatures ranged between (1) $15-23^{\circ}C$ and (2) $23-28^{\circ}C$. Consequently, it was shown that the black scraper is a warm water species and inhabiting waters in the temperature range from $15-28^{\circ}C$ is deemed appropriate.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.231-238
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• 2006

The physical properties of seven perlites different in particle size distribution were investigated to develop perlite bag culture in Korea. Particle sizes of 1.0-2.8mm and larger than 2.8 mm were rather evenly distributed in S-1 (1.2-5 mm), S-2 (0.15-5 mm) and S-5 (parat No.1). Larger particles were less in S-3 (1-3 mm), S-4 (Parat No.2), S-6 (OTAVI) and S-7 (Agroperl B-3). S-4, S-6 and S-7 contained lots of particles less than 1 mm in size. Total porosity was similar among substrates with the value of $59{\sim}62%$. Container capacity was between 35-40% regardless of substrates except in S-2 with 27.7%. Water content, which was about 60% at 0 kPa, was decreased sharply at 4.90 kPa regardless of substrates, which meant the easily available water was plenty in any kind of perlite tested. Substrates, S-1, S-2 and S-3 with different particle size distribution, were investigated to evaluate for perlite bag culture. Six tomatoes (Licopersicon esculentum Mill. cv. Rokkusanmaru) were planted in a perlite bag of 40 liters with the dimension of 120cm in length and 34cm in width. The amount of nutrient solution supplied and its drainage dependent on daily integrated radiation didn't show any regular trend during the growth. Roots in the bag were distributed evenly in S-1 and S-2 than in S-3. Plant grown in S-1 showed the highest total and marketable yield of 8,628 and 7,759 kg/10a, respectively. The number of small size fruits and malformed fruits were more in S-3. Consequently, S-1 with the particle size distribution of 1.2-5 mm is suggested as desirable substrate for perlite bag culture.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.359-369
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• 2011

Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed $10{\times}10^4$ algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.353-358
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• 2011

This study is the result of examining the growth and survival rate of larva and spat when supplied with Enriched live food by adding $B_{12}$, chitosan, PSB, and polysaccharides to microalgae (Chaetoceros gracilis, Isochrysis galbana) to raise the survival rate of larva and spat during artificial clam seed production. Microalge (Chaetoceros gracilis, Isochrysis galbana) was strengthened for nutrition with $B_{12}$ 0.1 ppm, chitosan 0.2 ppm, PSB 3 ppm and polysaccharides 1 ppm and was daily supplied for $3{\times}10^3-15{\times}10^3Cells/mL$ and the growth and survival rates were measured. As the result of experiment, the growth of larva did not show much difference with PSB test section $199{\pm}0.59{\mu}m$, $B_{12}$ test section $198{\pm}0.64{\mu}m$, and chitosan $197{\pm}0.52{\mu}m$, survival rate was highest at PSB test section with 99.3%, followed by $B_{12}$ test section 95.9%, and chitosan 94.5%. Growth of early spat was the highest for PSB test section at $2.74{\pm}0.58mm$, followed by polysaccharides $2.67{\pm}0.55mm$, $B_{12}$ $2.54{\pm}0.48mm$, and chitosan $2.49{\pm}0.51mm$, and the survival rate was the highest for PSB test group at 32.1%, followed by $B_{12}$ test section 31.6%, chitosan 28.5%, and polysacharrides 21.4%. From such results, PSB is found to be very effective with low-quality improvement when breeding early spat of clams, and especially for floor-type early spat breeding, it had the effect of suppressing protozoan and germs so that detailed studies from various perspectives should be conducted with various chemicals in the future.

• The Korean Journal of Malacology
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• v.30 no.3
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• pp.235-242
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• 2014

This study was conduct to investigate the effect of Intermediate culture types on the growth and survival rate of the abalone, Haliotis discus hannai, in net cage and indoor tank. Intermediate cultures were to determine there that was to setting at marine net cage culture (NCC) in net cage, floor culture (FC), net floor culture (NFC), double shelter culture (DSC) and indoor net cage culture (INCC) in indoor tank, in two replicate. In the growth performance of juvenile abalone reared through intermediate culture, that the absolute growth rate ($AGR_{SL}$, $AGR_{SB}$), daily growth rate ($DGR_{SL}$, $DGR_{SB}$), and specific growth rate ($SGR_{SL}$, $SGR_{SB}$) to the shell length $(_{SL})$ and shell breadth $(_{SB})$ of NCC were higher than those of different groups (P < 0.05). As weight gain (WG), daily weight gain (DWG) and specific weight gain (SWG) to body weight through intermediate culture types in indoor tank was not significant. Also that, survival rate among experimental groups of intermediate culture in indoor tank was not significant. Therefore, these results is showed that should to cultivate for net cage so that intermediate culture of juvenile abalone over 2 cm, accordingly research to effective progress of juvenile abalone intermediate culture in indoor tank be should from various reason as well as feed and rearing condition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.1
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• pp.49-54
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• 2017

We collected and reared Theragra chalcogramma walleye pollock brood-stock for use in natural spawning tests and undertook to obtain domestic pollock via fertilized egg capture, development of fertilized eggs, and absorption of yolk sac after hatching. Whole pollock were caught with trammel and set nets and immediately placed in a deep-sea water tank. Adults were the most common pollock age group (43.0%; n = 86) among the 254 pollock captured in March 2014 with 57.9% (n = 147) being captured off Southern Gosung, Korea. The main spawning period of pollock is February (spawning phase of 91% of pollock). From the deep-sea tank, we collected 1640 mL of naturally fertilized eggs (~820,000 eggs) from 12 spawning events occurring between February 4 and 22 2015. The floating/ live eggs were maintained in deep-sea water tanks at $5.5{\pm}0.2^{\circ}C$. Egg size was $1.5{\pm}0.03mm$. Six hours after fertilization the eggs were at the 2 cell stage, and the eggs hatched approximately 340 hours after collection. At hatching, larval length and yolk sac area were $5.2{\pm}0.25mm$ and $9.5{\pm}1.00mm^2$ (100%), respectively. Four days after hatching, the yolk sac area was $2.2{\pm}0.53mm^2$ ($23.1{\pm}5.55%$). This is the first report of collection of naturally fertilized eggs from pollock and their subsequent hatching while held in an indoor deep-sea water tank. The results suggest that such collection could assist in the recovery of pollock resources and the possibility of domestic rearing of cultivated larvae.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.4
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• pp.457-468
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• 1995

In order to develop a more practical culture system from the present running seawater tank system, two experiments of environmental factors, growth, survival rate and rearing density of olive flounder (Paralichthys olivaceus) were carried out for two consecutive years. Two groups of fish in initially averaging 7.5cm of total length, and 3.4g of body weight (EXP. I) and 5.0cm and 1.8g (EXP. II) were reared in the semi-closed recirculating seawater system equipped with the rotating biological contactors with the commercial culture scale. The dissolved inorganic nitrogen concentrations is EXP. I ranged 0,247-0.512 ppm of $NH_4-N$ (0.010-0.043 ppm of$NO_2-N$, and 0.108-0.342 ppm of $NO_3-N$, and those in EXP. II were 0.091-0.715 ppm, 0.002-0.045 ppm, and 0.007-0.277 ppm, respectively. Daily feeding rates of the fish were $0.67-2.41\%$ in EXP. I and $0.69-2.22\%$ in EXP_ II, and teed efficiency were $34.8-59.8\%\;and\;40.5-88.4\%$ in EXP. I and II, respectively. The average total ten說h and body weight were 40.0-42.8cm and 695.0-852.69g after 340 days culture in EXP. I, and 36.7-39.7cm and 552.4-706.4 g after 365 days culture in EXP. II, respectively. Survival rates of the fish at the end of EXP. I and II were $92.0\%\;and\;96.0\%,$ respectively. The ratio to body surface area of non-ocular side in all fish to bottom area of rearing tank, so-called covering rate, was used as an indicator of rearing density. The highest cowering rate and weight density of fish per $m^2$ of rearing tank at the end of experimental period were 2.2 and 34.1kg in EXP. I, and 2.6 and 36.3kg in EXP. II, respectively. For the commercial culture of olive flounder, the semi-closed recirculating seawater system was found to be more effective than the running seawater tank system in aspect to the fish productivity and protection of marine environment.

• The Korean Journal of Malacology
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• v.17 no.1
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• pp.35-44
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• 2001

This research was conducted to develop seedling production techniques in flat oyster, Ostrea dense lamellosa. The cultivation of larvae, artificial spat and spats collection in natural conditions rate were examined. In the mass culture tank (3 tons), average growth rates of the D Shape larvae of initial shell length (153.4 ㎛) and shell height (153.4 ㎛) were 202.6% and 212.9% at 16 days and 227.1%, 241.8% at 20 days, respectively. Instantaneous death and survival rate of the larvae were 0.160 and 54.8% at 16 days and 0.057 and 43.2% at 20 days, respectively. Collection rate of flat oyster from bottom using various collectors with oyster shell, scallop shell, ark shell and PVC plates were 32.9%, 24.1%, 16.8% and 10.0%, respectively. and the greatest collecting rate was 131.9 individuals/shell (32.9%) in laid collectors on the bottom. The collecting rate of the oyster larvae were better in laid collectors on the bottom 83.8 individuals/collection than in the suspended string 54.2 individuals/collection. in all collecting substrates. Early spats settled on collectors were grew to 2.38 $\pm$ 0.97 ㎜ in shell length and 2.16 $\pm$ 0.86 ㎜ In shell height at 26 days. The spats were grew to 28.58 $\pm$ 2.39 ㎜ in shell length and 31.65 $\pm$ 2.03 ㎜ in shell height during the 4 month mid-term cultivation. In the period of cultivation, the mean number of spats attached to collectors were 10.3 individuals at oyster shells, 5.8 scallop shells, 4.0 ark shells and 1.5 PVC plates, respectively.

• Journal of fish pathology
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• v.20 no.1
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• pp.41-47
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• 2007

To investigate the cause of the mass mortality during rockbream, Oplegnathus fasciatus seed production, the water quality and bacterial counts of sea water in breeding tanks was measured 20days post the hatch. During breeding of rockbream fry, the environmental factors of water quality were detected as pH, ammonia COD, phosphate at the supply of the food organisms and the seawater. pH was decreased from the 8.21 of the 1 day per hatch (dph) to 7.56 of the on the 7 dph. Ammonia was conversely increased 0.49 ppm of the 1 dph to 0.85 ppm of 10 dph. As the adding of the chlorella and the rotifer tanks, COD was increased the 3.3 times and 1.2 times than those of pre-adding respectively. The phosphate and the ammonia were also increased 1.7 and 2.3 times, with adding the chlorella respectively, which exceeded the second grade for sea water evaluation level, 0.015 ppm and 0.1 ppm respectively. Water quality was not improved by PSB (Photosynthetic Bacteria) treatment, which increased the value of COD in 1.7 times, phosphate in 2.7 times and ammonia in 1.4 times. The number of the bacteria was also increased along the dph. According to the treatment of chlorella, the number of total bacteria increased in 1.4 times and those of Vibrio sp. 1.6 times. The lethal concentration of ammonia was investigated that over than 10 ppm could killed the fry of rockbream within 28 hrs, but 40% in 2 ppm.

• Journal of Aquaculture
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• v.8 no.3
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• pp.195-207
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• 1995

In order to study the embryonic development and hatching of wild long shanny, Stichaeus grigorjewi, were caught with the gill nets in the East Sea of Korea, and stocked at indoor tanks to induce natural spawning in February 25, 1994 and February 16 to 24, 1995. They were already matured when stocked, and average body length (50.66 cm) and body weight (1,192.74 g) of 57 females and average body length (48.62 cm) and body weight (612.58g) of 43 males were recorded. Before stocking, they were inserted with identification tags(ID tags) in the dorsal muscle, and spawning was traced by the portable reader (Destron/lDl Ltd.) Forty females among 57 spawned successfully in the average of 4 days after stocking. Females spawned almost all eggs contained in the ovaries at one time in the form of an egg mass and averaging 227,200 eggs Per egg mass. The egg mass was oval in shape, translucent milky in color, 20.32cm long axis and 14.57cm short axis in size, and 803.7g in weight. Male parents guarded their egg masses and circulated water with the tail part of the body. Fertilized egg was spherical in shape, and their average diameter was 1.54 mm. Each egg had a containing single oil globule, and it's average diameter was 0.37 mm. The average water temperature was $13.2^{\circ}C$ and incubation times after fertilization were 5 hours 25 minutes up to 2-cell stage, 13 hours up to morula stage, and 66 hours 35 minutes up to embryo formation stage. Hatching rate was approximately 10 percent in 368 hours 50 minutes after fertilization, and approxionateoly 90 percent of eggs were hatched in 425 hours 30 minutes after fertilization.