• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.04a
• /
• pp.53-53
• /
• 2018

전라북도 동부권 지역은 중산악지형으로 표고 100~500m 산지가 69%, 500~1,000m가 26%로 이루어져 있어, 산채를 재배하기에 유리한 지리적 조건을 갖고 있다. 따라서 소비자의 수요가 높은 산채류의 재배 및 이용에 대한 농업인들의 기술개발이 요구되고 있다. 이에 전라북도 준고랭지역에서 재배하기 적합한 산채작목을 선발하고자 본 시험을 실시하였다. 시험은 표고 500m 전라북도 남원시 운봉읍 허브시험장에서 노지재배, 남원시 주천면 표고 530~540m, 진안군 주천면 표고 340~350m에 위치한 농가포장에서 임간재배를 실시하였다. 대상작물은 개미취(Aster tataricus), 갯기름나물(Peucedanum japonicum), 곤달비(Ligularia stenocephala), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 산마늘(Allium microdictyon), 섬쑥부쟁이(Aster glehnii), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata) 등 9종을 2016년 종묘를 정식하였다. 정식 2년차에 해당지역의 5월~9월 평균기온 및 조도 등 생육환경을 조사하고, 시험작물의 출현율, 생육특성, 수량 등을 조사하였다. 조사기간 동안의 평균기온은 노지가 $20.8^{\circ}C$, 남원임간은 $20.3^{\circ}C$, 진안임간은 $20.5^{\circ}C$이었으며, 평균조도는 노지가 37,362Lux, 남원임간은 7,816Lux, 진안임간은 25,316Lux이었다. 노지에서 출현율은 개미취, 갯기름나물, 눈개승마, 산마늘, 섬쑥부쟁이, 우산나물이, 임간에서는 개미취, 곤달미, 눈개승마, 단풍취, 산마늘, 어수리, 우산나물이 높았다. 생육특성은 노지에서 개미취, 갯기름나물, 눈개승마, 섬쑥부쟁이, 임간에서는 단풍취, 산마늘, 우산나물의 생육이 좋았다. 수량은 노지에서 개미취, 갯기름나물, 곤달비, 산마늘, 섬쑥부쟁이, 어수리가 높았으며, 남원임간에서는 곤달비, 단풍취, 우산나물, 어수리가, 진안임간에서는 개미취, 갯기름나물, 산마늘, 우산나물의 수량이 많았다. 이와 더불어 준고랭지에 적합한 산채류률 선발하기 위해서는 차후 재배 지역 및 방법의 차이에 따른 산채작물의 연차별 수량성과 경제성에 관한 연구가 이루어져야 할 것으로 여겨진다.

• Korean Journal of Ichthyology
• /
• v.20 no.4
• /
• pp.272-278
• /
• 2008

Optimum concentration of alizarine complexone (ALC) for staining of otoliths for Pacific cod was determined by examination of stained quality, growth and survival rates. Small juveniles ($34.1{\pm}1.8mm$ TL, $mean{\pm}SD$) were immersed in four different concentrations (20, 40, 60, 80 mg/L) and large ones ($73.8{\pm}1.2mm$ TL, $mean{\pm}SD$) in five different concentrations (1, 5, 10, 20, 40 mg/L) of ALC for 24 hrs. The fish were then reared and observed for 14 days. The stained quality of otoliths was evaluated by dividing them into four grades by the presence of fluorescent stains on the otoliths under G-excited UV light. The small juveniles stained with the different ALC concentrations had 100% staining success, and their growth rate and body weight did not differ significantly between treatments. Optimal ALC concentration for the smaller juveniles was 20 mg/L in the present experiment. On the other hand, distinct fluorescent rings were confirmed on otoliths of larger juveniles in the 10 mg/L treatment with high survival rate. These results suggest that ALC staining at concentrations of 10 mg/L with larger juveniles will be helpful for release-recapture experiments for stock enhancement of Pacific cod.

• Korean Journal of Ichthyology
• /
• v.20 no.4
• /
• pp.255-262
• /
• 2008

The endemic Korean stumpy bullhead Pseudobagrus brevicorpus is a first-grade endangered wild fish as designated by the Ministry of Environment of Korea. As part of its restoration and proliferation effort, a histological study of this fish was carried out to investigate sex differentiation and gonadal development based on F1 generation individuals obtained by artificial breeding. On days 4~5 after hatching, a pair of genital ridges including clusters of primordial germ cells was observed between the gut and the mesonephric duct. On days 20 after hatching, the ovary began to initially differentiate and contained early oocytes with chromatin-nucleolus and peri-nucleolus stages on days 30~40 after hatching. As yolk material accumulated after day 80 from hatching, the oocytes grew increasingly large and were surrounded by a distinct follicular layer. On days 306 after hatching, the oocytes grew toward a mature ovum. In the males, the testis was distinguished by emergence of spermatogonium cells on 25 days after hatching, and day 40 after hatching it contained a small number of seminal lobes forming cysts. From 173 days after hatching, the testis consisted of numerous enlarged seminal lobes including spermatocytes and spermatids. Over 14 months after hatching, some seminal lumens were filled with spermatozoa.

• Journal of Aquaculture
• /
• v.8 no.1
• /
• pp.59-67
• /
• 1995

Growth rate, first reproductive age, life span and total offspring numbers were measured to study the effects of water temperature and salinity on the propagation of rotifer, Brachionus plicatilis. Three types of rotifer, Large (L), Small (S) and Thailand Small (TS)-types, were cultured in the $4\times4$ factorial culture conditions with four different salinities (5, 15, 25 and $35\%o$) at each of four different water temperatures (15, 20, 25 and $29^{\circ}C$). The results are as follows; Under the 4 different salinity gradients %$(5\%o,\;15\%o,\;25\%o,\;and\;35\%o)\;at\;29^{\circ}C$, the mean growth rates of L, Sand TS-type were 0.60, 0.84 and 0.96, respectively. The first reproductive age of three types rotifer appeared to be early at high water temperature. The total offspring numbers of the three types were higher at $25^{\circ}C\;and\;29^{\circ}C$, with the maximum value of 28.3 on the average at $29^{\circ}C$ for TS-type, and the minimum value at $15^{\circ}C$ for S-type. Life span decreased with high water temperature and increased with low water temperature. L-type and TS-type rotifer showed the longest life span of 13.5 days on the average at $15^{\circ}C$, and S-type showed shortest 6.2 days on the average at $29^{\circ}C$.

• Journal of Aquaculture
• /
• v.10 no.1
• /
• pp.55-61
• /
• 1997

The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~400g, 1,500~2,000g and 2,500~4,000g, were collected from June to August, 1996. They were treated two or three times with HCG (500~1,000 IU/kg). Gonadal development of the sevenband groupers were examined using the paraffin section method and then stained with haematoxylin and eosin. When smaller groups of sevenband grouper having 300~400g, 1,500~2,00g and 2,500~3,200g in weight were treated two or three time with HCG 9500~1,000 IU/g), their gonads contained oogonia, oocytes with the size of 20~30${\mu}{\textrm}{m}$, and stroma tissues. However, in case of two HCG treatments with a dosage of 1,000 IU/kg on sevenband groupers of 3,400~4,000g, both healthy eggs of $800\mu$m in diameter and regressing eggs were ovulated. A sevenband grouper of 4,000g obvulated 4,252 eggs in total. Of these, healthy and regressing eggs turned out to be 20.8% and 79.2%, respectively. In addition, previtellogenic oocytes of around $50\mu$m, oocytes of 20~$30\mu$m, and ovulation trace were also observed in the gonad.

• The Journal of Fisheries Business Administration
• /
• v.34 no.1
• /
• pp.157-183
• /
• 2003

유류오염 사고를 사전에 예방할 수 있는 정책 수단으로는 여러 가지가 있지만 주요한 것으로는 인센티브제의 활용을 들 수 있다. 유류오염 사고를 예방하고 관리하기 위한 인센티브는 유출 사고로 인해 발생한 해양자원의 피해에 대해 가해자에게 배상책임(liability for losses due to spills)을 부과함으로써 제공될 수 있다. 유류오염 사고로 인한 피해액을 실제 화폐단위로 계량화하는 작업은 배상책임 부과제도를 정책수단으로 활용하기 위해 해결해야 할 가장 어려운 과제이다. 따라서, 최근 미국과 캐나다를 중심으로 발전하고 있는 자연자원 피해에 대한 가치 평가법(Natural Resource Damage Assessment : NRDA)은 배상책임 부과제도를 정책적으로 보완할 수 있는 이론적 도구로 간주되고 있다. NRDA는 잠재적인 가해자들에게 그들이 자연환경을 보존해야 하는 사회적 의무를 이행하지 못하고 이를 훼손하게 될 때 이로 인해 발생하는 모든 사회적 비용을 직접 부담해야 한다는 명확한 재무적 인센티브(financial incentive)를 부여함으로써 가해자 보상 원칙 (polluter pays principle)을 실현 할 수 있게 한다. 본 연구는, 유류오염 사고로 인한 환경자원 피해의 경제적 가치를 추정하는 가장 중요한 이론적 모형으로 활용되고 있는 가상상황평가법(CVM)에 대한 기초 개념과 이론적 체계, 그리고 이를 실제 피해액 추정에 성공적으로 적용시키기 위해 해결해야 할 문제점 등을 다루었다. 이를 위해, 본 연구에서는 1988년 캐나다 북서부 연안에서 발생한 Nestucca 유류오염 사고를 사례연구의 대상으로 선정하고, 사고 당시 캐나다 연방정부와 British Columbia 주정부를 대신하여 해양오염에 의한 환경피해의 경제적 가치를 추정한 미국의 컨설팅 회사인 RCG/Hagler, Baily Inc.의 가상상황평가법(CVM) 적용 사례를 분석 검토하였다. Nestucca 사례연구에서는 이들 연구자들이 실제로 활용한 설문지 설계, 설문방법 및 표본설계 등을 분석하였으며, 또한 CVM이 본질적으로 갖고 있는 방법론적 문제점들을 연구자들이 어떻게 해결하려고 했는가를 고찰하였다. 그리고, WTP 추정을 위해 RCG 연구자들이 사용한 사전규제접근법(ex ante regulatory approach)으로 인해 야기될 수 있는 환경자원 피해액 추정 방법의 한계점도 함께 검토하였다. 캐나다 연방정부와 British Columbia 주정부는 Nestucca 유류오염 사고로 인한 자연 자원 피해에 대한 손해배상으로 $4.3 Million의 보상금을 지급 받게 된다. 캐나다 정부는 이 보상금으로 Nestucca Oil Spill Trust Fund를 설립하여 피해를 입은 자연자원의 원상회복(restoration)을 위한 다양한 연구 프로젝트에 자금을 지원하고 있다. Nestucca 유류오염 사고를 계기로 캐나다 정부와 학계는 해양자원의 피해에 대한 경제적 가치평가와 자원의 원상회복에 대한 체계적인 접근 방안을 처음으로 마련 시행하게 되었다는 점에서, Nestucca 유류오염 사고에 대한 사례연구는 캐나다의 해양환경 보존 정책을 연구하는 출발점으로 평가될 수 있을 것이다. 이에 비해, 우리나라에서 대표적인 유류오염사고로 알려져 있는 시프린스호 사고와 관련된 손해배상금은 주로 연안어민들의 어업피해 배상으로 이루어져 있으며, 간접피해에 대한 배상액 48억 5천만원도 대부분 치어방류, 여수대학교 종묘배양장건립 등 피해지역 연안어업 발전을 위한 사업에 투자되었다.

• The Korean Journal of Malacology
• /
• v.17 no.2
• /
• pp.79-84
• /
• 2001

In order to obtain the basic information for seedling production of the scallop, Patinopecten yessoensis, the egg development and larva growth were investigated at different conditions such as water temperature, salinity and phytoplankton. Eggs were demersal isolated eggs, which averaged 77.3${\pm}$2.7$\mu\textrm{m}$ in diameter after spawning. The fertilized eggs developed to D-shaped larva of shell length 117.5${\pm}$3.8$\mu\textrm{m}$ after 60 hours at 15$^{\circ}C$. The range of water temperature and salinity during egg development were 10-20$^{\circ}C$ and 28-34 ppt, respectively. The time of egg development was shorter with higher water temperature. After 10 days of spawning, D-shaped larva reached 160 $\mu\textrm{m}$ in shell length, and after 25 days became full-grown larva 250 $\mu\textrm{m}$ in shell length, in which could be observed eye spots. The relative growth formula between shell length (SL) and shell height (SH) was SH = 1.0425SL-27.731 (r$^2$= 0.9749) during the entire larva period. In regard to water temperature, growth and survival rates of larvae were good at 16$^{\circ}C$. Lower growth and survival rates were observed at 12$^{\circ}C$ and 20$^{\circ}C$ than that at 16$^{\circ}C$. When larvae were fed mixed phytoplankters, such as isochrysis galbana, Pavlova lutheri and Chaetoceros calcitrans, their growth and survival rates were the highest among groups.

• Journal of Aquaculture
• /
• v.10 no.4
• /
• pp.463-472
• /
• 1997

Floating and hatching rates, size and survival activity index(SAI) of spawned eggs of red spotted grouper, Epinephelus akaara were studied to understand egg qualities during the spawning periods in 1992, 1993 and 1995. The fish spawned from mid-July to mid-August when temperature ranged 23~$28^\circC$. Floating, fertilization and hatching rates were higher in the eggs from early to middle than those from late during the spawning period. SAI ranged 1.8~9.6, and it was closely related to survival rate until mouth operning time (SMOT). Egg diameter decreased gradually with the time of spawning, while diameter of oil globule was constant during the spawning periods. Induced spawnings were carried out with one injection of HCG over 5, 000 IU/kg body weight. Floating, fertilization and hatching rates of the induced spawning eggs were higher than those of untreated ones. Thus, induced spawning method with HCG was recommended for collecting fertilized eggs in bulk. Optimal time for the collection of high quality eggs was supposed to be from early to middle during the spawning periods. Considering that egg qualities were vulnerable within the period, hatchability, incidence of malformed larvae, SMOT and SAI were factors to be considered together to get good quality of eggs.

• The Korean Journal of Malacology
• /
• v.28 no.1
• /
• pp.7-12
• /
• 2012

The effect of water temperature on spawning induction, larval development, spat settling and its growth of the cockle shell, Fulvia mutica, were investigated to obtain the basic data for effective seed production. The eggs, which were randomly divided into 6 groups of water temperatures of 14, 17, 20, 23, 26 and $29^{\circ}C$, were transferred into 1 L beaker, respectively. The relationships between the water temperature and the required time (1/h, hour) by each egg developmental stage were calculated. Biological minimum water temperature and the cumulative water temperature until egg development of the veliger stage were calculated to be $0.1^{\circ}C$ and $397.3^{\circ}C$, respectively. The optimal water temperature for developmental bioassay of F. mutica was clarified to be $23^{\circ}C$. The required time for the embryo to become veliger larvae was 20 hours at $23^{\circ}C$.

• Korean Journal of Medicinal Crop Science
• /
• v.1 no.2
• /
• pp.129-136
• /
• 1993

For the clonal proliferation of Gentiana scabra Bunge. which is one of the medicinaland ornamental plant, establishment multiplication of shoot through tissue culture technique and transplantation into soil were carried out. The shoot proliferation increased on the MS medium containing 0.5mg/l NAA and 0.5mg/l BAP. Optimum pH for shoot growth was pH 5.9, consequently MS medium supplemented with 2g/l activated charcoal was most effective for plant growth. There are two types of somaclonal variants, tall type was 63% and dwarf type was 37%.